Restriction map for
pFN21AE0957
(Linear) (Six-base) MAPSORT of: KIEE0957.seq Check: 188 from: 1 to: 1479
KIEE0957.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 162 1479
Size: 162 1317
AccI GT'mk_AC
Cuts at: 0 414 546 1479
Size: 414 132 933
Fragments arranged by size:
933 414 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 574 1253 1479
Size: 97 477 679 226
Fragments arranged by size:
679 477 226 97
AfeI AGC'GCT
Cuts at: 0 23 1479
Size: 23 1456
ApoI r'AATT_y
Cuts at: 0 710 1479
Size: 710 769
AvaI C'yCGr_G
Cuts at: 0 5 1479
Size: 5 1474
BanI G'GyrC_C
Cuts at: 0 1263 1479
Size: 1263 216
BanII G_rGCy'C
Cuts at: 0 65 829 1479
Size: 65 764 650
Fragments arranged by size:
764 650 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 1444 1472 1479
Size: 210 1234 28 7
Fragments arranged by size:
1234 210 28 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1339 1479
Size: 1339 140
BclI T'GATC_A
Cuts at: 0 176 875 1479
Size: 176 699 604
Fragments arranged by size:
699 604 176
BglII A'GATC_T
Cuts at: 0 504 1479
Size: 504 975
BlpI GC'TnA_GC
Cuts at: 0 18 1479
Size: 18 1461
BmrI ACTGGGnnnn_n'
Cuts at: 0 1203 1479
Size: 1203 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1479
Size: 660 819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 1479
Size: 197 1282
BsaI GGTCTCn'nnnn_
Cuts at: 0 435 1479
Size: 435 1044
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1214 1244 1479
Size: 1214 30 235
Fragments arranged by size:
1214 235 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 663 1249 1479
Size: 663 586 230
Fragments arranged by size:
663 586 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1479
Size: 274 1205
BsiEI CG_ry'CG
Cuts at: 0 56 1479
Size: 56 1423
BsiHKAI G_wGCw'C
Cuts at: 0 65 424 1479
Size: 65 359 1055
Fragments arranged by size:
1055 359 65
BsmI GAATG_Cn'
Cuts at: 0 213 259 1479
Size: 213 46 1220
Fragments arranged by size:
1220 213 46
Bsp1286I G_dGCh'C
Cuts at: 0 65 424 829 1479
Size: 65 359 405 650
Fragments arranged by size:
650 405 359 65
BspHI T'CATG_A
Cuts at: 0 816 1479
Size: 816 663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 162 957 1479
Size: 124 38 795 522
Fragments arranged by size:
795 522 124 38
BsrBI CCG'CTC
Cuts at: 0 1408 1479
Size: 1408 71
BsrDI GCAATG_nn'
Cuts at: 0 755 1479
Size: 755 724
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 1340 1479
Size: 339 1001 139
Fragments arranged by size:
1001 339 139
BstYI r'GATC_y
Cuts at: 0 504 532 1272 1479
Size: 504 28 740 207
Fragments arranged by size:
740 504 207 28
BtgI C'CryG_G
Cuts at: 0 332 863 1479
Size: 332 531 616
Fragments arranged by size:
616 531 332
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 400 1479
Size: 400 1079
BtsI GCAGTG_nn'
Cuts at: 0 286 1479
Size: 286 1193
EaeI y'GGCC_r
Cuts at: 0 53 341 1479
Size: 53 288 1138
Fragments arranged by size:
1138 288 53
EagI C'GGCC_G
Cuts at: 0 53 1479
Size: 53 1426
EarI CTCTTCn'nnn_
Cuts at: 0 1430 1479
Size: 1430 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 16 1479
Size: 16 1463
EcoICRI GAG'CTC
Cuts at: 0 63 1479
Size: 63 1416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 574 660 1253 1479
Size: 97 477 86 593 226
Fragments arranged by size:
593 477 226 97 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1002 1479
Size: 1002 477
HaeII r_GCGC'y
Cuts at: 0 13 25 889 1479
Size: 13 12 864 590
Fragments arranged by size:
864 590 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 958 990 1154 1186 1313 1345 1431 1463
Size: 958 32 164 32 127 32 86 32
Cuts at: 1463 1479
Size: 16
Fragments arranged by size:
958 164 127 86 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 415 1479
Size: 415 1064
MfeI C'AATT_G
Cuts at: 0 569 893 1243 1479
Size: 569 324 350 236
Fragments arranged by size:
569 350 324 236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 322 1200 1479
Size: 257 65 878 279
Fragments arranged by size:
878 279 257 65
MscI TGG'CCA
Cuts at: 0 343 1479
Size: 343 1136
MspA1I CmG'CkG
Cuts at: 0 52 1479
Size: 52 1427
NcoI C'CATG_G
Cuts at: 0 332 863 1479
Size: 332 531 616
Fragments arranged by size:
616 531 332
NdeI CA'TA_TG
Cuts at: 0 920 1479
Size: 920 559
NotI GC'GGCC_GC
Cuts at: 0 53 1479
Size: 53 1426
NspI r_CATG'y
Cuts at: 0 137 407 1479
Size: 137 270 1072
Fragments arranged by size:
1072 270 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 325 1479
Size: 325 1154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 806 1479
Size: 774 32 673
Fragments arranged by size:
774 673 32
SacI G_AGCT'C
Cuts at: 0 65 1479
Size: 65 1414
SalI G'TCGA_C
Cuts at: 0 413 1479
Size: 413 1066
SapI GCTCTTCn'nnn_
Cuts at: 0 1430 1479
Size: 1430 49
SfcI C'TryA_G
Cuts at: 0 426 1479
Size: 426 1053
SmaI CCC'GGG
Cuts at: 0 7 1479
Size: 7 1472
SmlI C'TyrA_G
Cuts at: 0 212 1479
Size: 212 1267
SphI G_CATG'C
Cuts at: 0 407 1479
Size: 407 1072
SspI AAT'ATT
Cuts at: 0 668 1479
Size: 668 811
StyI C'CwwG_G
Cuts at: 0 332 747 863 1479
Size: 332 415 116 616
Fragments arranged by size:
616 415 332 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1101 1479
Size: 1101 378
XmaI C'CCGG_G
Cuts at: 0 5 1479
Size: 5 1474
XmnI GAAnn'nnTTC
Cuts at: 0 667 1479
Size: 667 812
Enzymes that do cut:
AarI AccI AcuI AfeI ApoI AvaI BanI BanII
BbsI BciVI BclI BglII BlpI BmrI BpmI BpuEI
BsaI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BstXI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI FalI
HaeII Hin4I HincII MfeI MmeI MscI MspA1I NcoI
NdeI NotI NspI PflMI PpiI SacI SalI SapI
SfcI SmaI SmlI SphI SspI StyI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI
Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseYI BsiWI BsmBI BspEI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MluI MslI
NaeI NarI NgoMIV NheI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI ZraI