Restriction map for
pFN21AE0955
(Linear) (Six-base) MAPSORT of: KIEE0955.seq Check: 2496 from: 1 to: 1449
KIEE0955.orf
With 182 enzymes: SgfI *
March 22, 2012 15:49 ..
AccI GT'mk_AC
Cuts at: 0 1414 1449
Size: 1414 35
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1449
Size: 1257 192
AfeI AGC'GCT
Cuts at: 0 337 1449
Size: 337 1112
AflIII A'CryG_T
Cuts at: 0 671 1449
Size: 671 778
AhdI GACnn_n'nnGTC
Cuts at: 0 1410 1449
Size: 1410 39
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 1048 1080 1277 1309 1449
Size: 448 32 568 32 197 32 140
Fragments arranged by size:
568 448 197 140 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 747 1449
Size: 747 702
ApaI G_GGCC'C
Cuts at: 0 861 1449
Size: 861 588
AvaI C'yCGr_G
Cuts at: 0 39 186 265 595 1019 1449
Size: 39 147 79 330 424 430
Fragments arranged by size:
430 424 330 147 79 39
BanI G'GyrC_C
Cuts at: 0 133 1449
Size: 133 1316
BanII G_rGCy'C
Cuts at: 0 40 74 193 824 861 869 938 1449
Size: 40 34 119 631 37 8 69 511
Fragments arranged by size:
631 511 119 69 40 37 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 35 110 131 1449
Size: 35 75 21 1318
Fragments arranged by size:
1318 75 35 21
Bme1580I G_kGCm'C
Cuts at: 0 596 861 1351 1449
Size: 596 265 490 98
Fragments arranged by size:
596 490 265 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 525 1171 1449
Size: 436 89 646 278
Fragments arranged by size:
646 436 278 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1152 1353 1449
Size: 760 392 201 96
Fragments arranged by size:
760 392 201 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 1449
Size: 412 1037
BsaHI Gr'CG_yC
Cuts at: 0 176 1449
Size: 176 1273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 691 721 1048 1078 1374 1404 1449
Size: 691 30 327 30 296 30 45
Fragments arranged by size:
691 327 296 45 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 104 236 928 1316 1434 1449
Size: 104 132 692 388 118 15
Fragments arranged by size:
692 388 132 118 104 15
BseYI C'CCAG_C
Cuts at: 0 276 1005 1232 1341 1449
Size: 276 729 227 109 108
Fragments arranged by size:
729 276 227 109 108
BsiEI CG_ry'CG
Cuts at: 0 1418 1449
Size: 1418 31
BsiHKAI G_wGCw'C
Cuts at: 0 193 869 938 1449
Size: 193 676 69 511
Fragments arranged by size:
676 511 193 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1307 1449
Size: 1307 142
Bsp1286I G_dGCh'C
Cuts at: 0 40 74 193 596 824 861 869 938
Size: 40 34 119 403 228 37 8 69
Cuts at: 938 1351 1449
Size: 413 98
Fragments arranged by size:
413 403 228 119 98 69 40 37
34 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1449
Size: 755 694
BsrBI CCG'CTC
Cuts at: 0 5 1449
Size: 5 1444
BsrDI GCAATG_nn'
Cuts at: 0 384 1449
Size: 384 1065
BsrFI r'CCGG_y
Cuts at: 0 136 1449
Size: 136 1313
BsrGI T'GTAC_A
Cuts at: 0 668 1449
Size: 668 781
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 1449
Size: 489 960
BstEII G'GTnAC_C
Cuts at: 0 297 1449
Size: 297 1152
BtgI C'CryG_G
Cuts at: 0 164 1449
Size: 164 1285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1113 1233 1449
Size: 1113 120 216
Fragments arranged by size:
1113 216 120
BtsI GCAGTG_nn'
Cuts at: 0 504 764 801 1449
Size: 504 260 37 648
Fragments arranged by size:
648 504 260 37
ClaI AT'CG_AT
Cuts at: 0 902 1449
Size: 902 547
DrdI GACnn_nn'nnGTC
Cuts at: 0 769 1449
Size: 769 680
EarI CTCTTCn'nnn_
Cuts at: 0 688 1270 1449
Size: 688 582 179
Fragments arranged by size:
688 582 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 101 158 233 1449
Size: 95 6 57 75 1216
Fragments arranged by size:
1216 95 75 57 6
EcoICRI GAG'CTC
Cuts at: 0 191 867 936 1449
Size: 191 676 69 513
Fragments arranged by size:
676 513 191 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 525 1171 1257 1449
Size: 436 89 646 86 192
Fragments arranged by size:
646 436 192 89 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 20 1449
Size: 20 1429
EcoO109I rG'GnC_Cy
Cuts at: 0 182 235 858 1337 1449
Size: 182 53 623 479 112
Fragments arranged by size:
623 479 182 112 53
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 580 1449
Size: 580 869
FspI TGC'GCA
Cuts at: 0 1196 1449
Size: 1196 253
FspAI rTGC'GCAy
Cuts at: 0 1196 1449
Size: 1196 253
HaeII r_GCGC'y
Cuts at: 0 339 1449
Size: 339 1110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 877 895 909 927 1065 1097 1396 1428
Size: 877 18 14 18 138 32 299 32
Cuts at: 1428 1449
Size: 21
Fragments arranged by size:
877 299 138 32 32 21 18 18
14
HincII GTy'rAC
Cuts at: 0 641 1415 1449
Size: 641 774 34
Fragments arranged by size:
774 641 34
HindIII A'AGCT_T
Cuts at: 0 1376 1449
Size: 1376 73
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1320 1417 1449
Size: 1320 97 32
Fragments arranged by size:
1320 97 32
MslI CAynn'nnrTG
Cuts at: 0 367 1000 1449
Size: 367 633 449
Fragments arranged by size:
633 449 367
MspA1I CmG'CkG
Cuts at: 0 215 230 290 916 984 1236 1345 1449
Size: 215 15 60 626 68 252 109 104
Fragments arranged by size:
626 252 215 109 104 68 60 15
NcoI C'CATG_G
Cuts at: 0 164 1449
Size: 164 1285
NspI r_CATG'y
Cuts at: 0 675 1449
Size: 675 774
PasI CC'CwG_GG
Cuts at: 0 31 711 961 1449
Size: 31 680 250 488
Fragments arranged by size:
680 488 250 31
PciI A'CATG_T
Cuts at: 0 671 1449
Size: 671 778
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 987 1449
Size: 348 639 462
Fragments arranged by size:
639 462 348
PfoI T'CCnGG_A
Cuts at: 0 452 1449
Size: 452 997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1449
Size: 1048 32 369
Fragments arranged by size:
1048 369 32
PpuMI rG'GwC_Cy
Cuts at: 0 1337 1449
Size: 1337 112
PshAI GACnn'nnGTC
Cuts at: 0 304 1449
Size: 304 1145
PspOMI G'GGCC_C
Cuts at: 0 857 1449
Size: 857 592
PspXI vC'TCGA_Gb
Cuts at: 0 186 1449
Size: 186 1263
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1449
Size: 1033 32 384
Fragments arranged by size:
1033 384 32
PstI C_TGCA'G
Cuts at: 0 485 1449
Size: 485 964
PvuII CAG'CTG
Cuts at: 0 916 984 1236 1449
Size: 916 68 252 213
Fragments arranged by size:
916 252 213 68
SacI G_AGCT'C
Cuts at: 0 193 869 938 1449
Size: 193 676 69 511
Fragments arranged by size:
676 511 193 69
SalI G'TCGA_C
Cuts at: 0 1413 1449
Size: 1413 36
SapI GCTCTTCn'nnn_
Cuts at: 0 688 1449
Size: 688 761
SfcI C'TryA_G
Cuts at: 0 481 1449
Size: 481 968
SgrAI Cr'CCGG_yG
Cuts at: 0 136 1449
Size: 136 1313
SmaI CCC'GGG
Cuts at: 0 267 1021 1449
Size: 267 754 428
Fragments arranged by size:
754 428 267
SmlI C'TyrA_G
Cuts at: 0 186 775 1167 1332 1449
Size: 186 589 392 165 117
Fragments arranged by size:
589 392 186 165 117
SpeI A'CTAG_T
Cuts at: 0 429 1449
Size: 429 1020
SspI AAT'ATT
Cuts at: 0 513 1449
Size: 513 936
StuI AGG'CCT
Cuts at: 0 1189 1449
Size: 1189 260
StyI C'CwwG_G
Cuts at: 0 164 324 629 1449
Size: 164 160 305 820
Fragments arranged by size:
820 305 164 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 1432 1449
Size: 157 1275 17
Fragments arranged by size:
1275 157 17
TatI w'GTAC_w
Cuts at: 0 668 924 1154 1449
Size: 668 256 230 295
Fragments arranged by size:
668 295 256 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 1449
Size: 1321 32 96
Fragments arranged by size:
1321 96 32
XhoI C'TCGA_G
Cuts at: 0 186 1449
Size: 186 1263
XmaI C'CCGG_G
Cuts at: 0 265 1019 1449
Size: 265 754 430
Fragments arranged by size:
754 430 265
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AloI AlwNI ApaI
AvaI BanI BanII BglI Bme1580I BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BtgI
BtgZI BtsI ClaI DrdI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NcoI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuII SacI SalI SapI SfcI SgrAI SmaI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaWI BsgI BsiWI BsmI BspEI BspHI BssHII BssSI
BstBI BstXI BstYI BstZ17I Bsu36I CspCI DraI DraIII
EaeI EagI EcoRI EcoRV FseI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PsiI PvuI
RsrII SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SphI SrfI SwaI Tth111I XbaI XcmI
XmnI ZraI