(Linear) (Six-base) MAPSORT of: KIEE0917.seq  Check: 6404  from: 1  to: 864



KIEE0917.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:33  ..

AccI GT'mk_AC

Cuts at:      0     273     304     864 
   Size:        273      31     560

  Fragments arranged by size: 

                 560     273      31
 
AhdI GACnn_n'nnGTC

Cuts at:      0     459     864 
   Size:        459     405
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116     864 
   Size:        116     748
 
ApoI r'AATT_y

Cuts at:      0     614     864 
   Size:        614     250
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     811     864 
   Size:        811      53
 
BglII A'GATC_T

Cuts at:      0     396     835     864 
   Size:        396     439      29

  Fragments arranged by size: 

                 439     396      29
 
BlpI GC'TnA_GC

Cuts at:      0     373     864 
   Size:        373     491
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     113     221     621     864 
   Size:        113     108     400     243

  Fragments arranged by size: 

                 400     243     113     108
 
BsaAI yAC'GTr

Cuts at:      0     244     864 
   Size:        244     620
 
BsaBI GATnn'nnATC

Cuts at:      0     162     822     840     864 
   Size:        162     660      18      24

  Fragments arranged by size: 

                 660     162      24      18
 
BsaHI Gr'CG_yC

Cuts at:      0     315     864 
   Size:        315     549
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     737     740     749     752     755     864 
   Size:        737       3       9       3       3     109

  Fragments arranged by size: 

                 737     109       9       3       3       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      69     864 
   Size:         69     795
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     864 
   Size:         69     795
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239     864 
   Size:        239     625
 
BsrBI CCG'CTC

Cuts at:      0      33     227     864 
   Size:         33     194     637

  Fragments arranged by size: 

                 637     194      33
 
BsrDI GCAATG_nn'

Cuts at:      0     428     864 
   Size:        428     436
 
BsrGI T'GTAC_A

Cuts at:      0     380     864 
   Size:        380     484
 
BstEII G'GTnAC_C

Cuts at:      0     788     864 
   Size:        788      76
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     543     864 
   Size:        543     321
 
BstYI r'GATC_y

Cuts at:      0     175     396     835     864 
   Size:        175     221     439      29

  Fragments arranged by size: 

                 439     221     175      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     152     864 
   Size:        152     712
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     445     477     864 
   Size:         22      32     391      32     387

  Fragments arranged by size: 

                 391     387      32      32      22
 
MspA1I CmG'CkG

Cuts at:      0      11     864 
   Size:         11     853
 
PpuMI rG'GwC_Cy

Cuts at:      0     152     864 
   Size:        152     712
 
SfcI C'TryA_G

Cuts at:      0     705     804     864 
   Size:        705      99      60

  Fragments arranged by size: 

                 705      99      60
 
StyI C'CwwG_G

Cuts at:      0     116     515     864 
   Size:        116     399     349

  Fragments arranged by size: 

                 399     349     116
 
TatI w'GTAC_w

Cuts at:      0     380     864 
   Size:        380     484
 
XbaI T'CTAG_A

Cuts at:      0     172     864 
   Size:        172     692
 

 Enzymes that do cut:

     AccI     AhdI     AlfI     ApoI    BciVI    BglII     BlpI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrGI   BstEII    BstXI    BstYI EcoO109I    Hin4I   MspA1I
    PpuMI     SfcI     StyI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrFI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI