Restriction map for
pFN21AE0912
(Linear) (Six-base) MAPSORT of: KIEE0912.seq Check: 6916 from: 1 to: 738
KIEE0912.orf
With 182 enzymes: SgfI *
March 22, 2012 15:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 501 738
Size: 501 237
Acc65I G'GTAC_C
Cuts at: 0 437 738
Size: 437 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 738
Size: 680 58
AlwNI CAG_nnn'CTG
Cuts at: 0 267 588 738
Size: 267 321 150
Fragments arranged by size:
321 267 150
ApoI r'AATT_y
Cuts at: 0 21 738
Size: 21 717
BamHI G'GATC_C
Cuts at: 0 447 738
Size: 447 291
BanI G'GyrC_C
Cuts at: 0 437 738
Size: 437 301
BanII G_rGCy'C
Cuts at: 0 209 482 738
Size: 209 273 256
Fragments arranged by size:
273 256 209
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 397 738
Size: 318 79 341
Fragments arranged by size:
341 318 79
BglI GCCn_nnn'nGGC
Cuts at: 0 124 133 178 738
Size: 124 9 45 560
Fragments arranged by size:
560 124 45 9
Bme1580I G_kGCm'C
Cuts at: 0 612 738
Size: 612 126
BmgBI CAC'GTC
Cuts at: 0 713 738
Size: 713 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 738
Size: 559 179
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 738
Size: 49 689
BsaHI Gr'CG_yC
Cuts at: 0 650 738
Size: 650 88
BsaWI w'CCGG_w
Cuts at: 0 493 738
Size: 493 245
BseYI C'CCAG_C
Cuts at: 0 32 463 645 738
Size: 32 431 182 93
Fragments arranged by size:
431 182 93 32
BsiEI CG_ry'CG
Cuts at: 0 668 738
Size: 668 70
BsiHKAI G_wGCw'C
Cuts at: 0 209 482 738
Size: 209 273 256
Fragments arranged by size:
273 256 209
Bsp1286I G_dGCh'C
Cuts at: 0 209 482 612 738
Size: 209 273 130 126
Fragments arranged by size:
273 209 130 126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 431 449 501 573 738
Size: 431 18 52 72 165
Fragments arranged by size:
431 165 72 52 18
BsrBI CCG'CTC
Cuts at: 0 71 553 738
Size: 71 482 185
Fragments arranged by size:
482 185 71
BssSI C'ACGA_G
Cuts at: 0 167 738
Size: 167 571
BstYI r'GATC_y
Cuts at: 0 447 738
Size: 447 291
BtgI C'CryG_G
Cuts at: 0 113 738
Size: 113 625
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 738
Size: 89 649
BtsI GCAGTG_nn'
Cuts at: 0 396 738
Size: 396 342
EaeI y'GGCC_r
Cuts at: 0 41 665 689 738
Size: 41 624 24 49
Fragments arranged by size:
624 49 41 24
EagI C'GGCC_G
Cuts at: 0 665 738
Size: 665 73
EarI CTCTTCn'nnn_
Cuts at: 0 59 738
Size: 59 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 634 738
Size: 634 104
EcoICRI GAG'CTC
Cuts at: 0 207 480 738
Size: 207 273 258
Fragments arranged by size:
273 258 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 680 738
Size: 559 121 58
Fragments arranged by size:
559 121 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 738
Size: 592 146
EcoO109I rG'GnC_Cy
Cuts at: 0 135 322 585 738
Size: 135 187 263 153
Fragments arranged by size:
263 187 153 135
HaeII r_GCGC'y
Cuts at: 0 87 268 738
Size: 87 181 470
Fragments arranged by size:
470 181 87
KpnI G_GTAC'C
Cuts at: 0 441 738
Size: 441 297
MspA1I CmG'CkG
Cuts at: 0 9 360 738
Size: 9 351 378
Fragments arranged by size:
378 351 9
PasI CC'CwG_GG
Cuts at: 0 318 738
Size: 318 420
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 738
Size: 39 699
PfoI T'CCnGG_A
Cuts at: 0 218 738
Size: 218 520
PpuMI rG'GwC_Cy
Cuts at: 0 322 585 738
Size: 322 263 153
Fragments arranged by size:
322 263 153
PstI C_TGCA'G
Cuts at: 0 263 446 561 738
Size: 263 183 115 177
Fragments arranged by size:
263 183 177 115
PvuII CAG'CTG
Cuts at: 0 9 738
Size: 9 729
SacI G_AGCT'C
Cuts at: 0 209 482 738
Size: 209 273 256
Fragments arranged by size:
273 256 209
SbfI CC_TGCA'GG
Cuts at: 0 446 738
Size: 446 292
SfcI C'TryA_G
Cuts at: 0 259 442 557 738
Size: 259 183 115 181
Fragments arranged by size:
259 183 181 115
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 124 133 738
Size: 124 9 605
Fragments arranged by size:
605 124 9
StyI C'CwwG_G
Cuts at: 0 239 738
Size: 239 499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 563 738
Size: 563 175
XmnI GAAnn'nnTTC
Cuts at: 0 352 738
Size: 352 386
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApoI BamHI BanI BanII
BbsI BglI Bme1580I BmgBI BpmI BsaI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstYI
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KpnI MspA1I PasI PflMI
PfoI PpuMI PstI PvuII SacI SbfI SfcI SfiI
StyI TaqII XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI