(Linear) (Six-base) MAPSORT of: KIEE0892.seq  Check: 4880  from: 1  to: 972



KIEE0892.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     727     972 
   Size:        727     245
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     972 
   Size:         83     889
 
AflIII A'CryG_T

Cuts at:      0     474     972 
   Size:        474     498
 
AleI CACnn'nnGTG

Cuts at:      0     739     972 
   Size:        739     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     419     694     972 
   Size:        419     275     278

  Fragments arranged by size: 

                 419     278     275
 
ApoI r'AATT_y

Cuts at:      0      31     659     972 
   Size:         31     628     313

  Fragments arranged by size: 

                 628     313      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      71     972 
   Size:         71     901
 
BbvCI CC'TCA_GC

Cuts at:      0     687     972 
   Size:        687     285
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     687     972 
   Size:        187     500     285

  Fragments arranged by size: 

                 500     285     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     972 
   Size:        661     311
 
BsaBI GATnn'nnATC

Cuts at:      0     335     972 
   Size:        335     637
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     464     494     972 
   Size:        464      30     478

  Fragments arranged by size: 

                 478     464      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      42     558     972 
   Size:         42     516     414

  Fragments arranged by size: 

                 516     414      42
 
BsmI GAATG_Cn'

Cuts at:      0     436     972 
   Size:        436     536
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     727     972 
   Size:        727     245
 
BsrGI T'GTAC_A

Cuts at:      0     505     972 
   Size:        505     467
 
BssSI C'ACGA_G

Cuts at:      0     244     972 
   Size:        244     728
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     651     972 
   Size:        651     321
 
EaeI y'GGCC_r

Cuts at:      0     353     593     972 
   Size:        353     240     379

  Fragments arranged by size: 

                 379     353     240
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     972 
   Size:         83     889
 
EcoRI G'AATT_C

Cuts at:      0     659     972 
   Size:        659     313
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     108     140     909     941     972 
   Size:        108      32     769      32      31

  Fragments arranged by size: 

                 769     108      32      32      31
 
HincII GTy'rAC

Cuts at:      0     543     839     972 
   Size:        543     296     133

  Fragments arranged by size: 

                 543     296     133
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     972 
   Size:         35     937
 
MscI TGG'CCA

Cuts at:      0     355     595     972 
   Size:        355     240     377

  Fragments arranged by size: 

                 377     355     240
 
MslI CAynn'nnrTG

Cuts at:      0     115     403     739     972 
   Size:        115     288     336     233

  Fragments arranged by size: 

                 336     288     233     115
 
MspA1I CmG'CkG

Cuts at:      0     365     694     972 
   Size:        365     329     278

  Fragments arranged by size: 

                 365     329     278
 
NspI r_CATG'y

Cuts at:      0     478     972 
   Size:        478     494
 
PciI A'CATG_T

Cuts at:      0     474     972 
   Size:        474     498
 
PstI C_TGCA'G

Cuts at:      0     724     972 
   Size:        724     248
 
PvuII CAG'CTG

Cuts at:      0     694     972 
   Size:        694     278
 
SexAI A'CCwGG_T

Cuts at:      0     104     972 
   Size:        104     868
 
SfcI C'TryA_G

Cuts at:      0     720     864     972 
   Size:        720     144     108

  Fragments arranged by size: 

                 720     144     108
 
SmlI C'TyrA_G

Cuts at:      0     676     972 
   Size:        676     296
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      47     824     972 
   Size:         47     777     148

  Fragments arranged by size: 

                 777     148      47
 
TatI w'GTAC_w

Cuts at:      0     505     972 
   Size:        505     467
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     972 
   Size:         35     937
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApoI     BbsI    BbvCI
   Bpu10I    BpuEI    BsaBI    BsaXI    BseRI     BsmI    BspMI    BsrGI
    BssSI    BtgZI     EaeI  Eco57MI    EcoRI    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NspI     PciI     PstI    PvuII    SexAI
     SfcI     SmlI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI