Restriction map for
pFN21AE0806
(Linear) (Six-base) MAPSORT of: KIEE0806.seq Check: 7086 from: 1 to: 1089
KIEE0806.orf
With 182 enzymes: SgfI *
March 22, 2012 15:48 ..
AccI GT'mk_AC
Cuts at: 0 368 1089
Size: 368 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 522 1089
Size: 508 14 567
Fragments arranged by size:
567 508 14
AleI CACnn'nnGTG
Cuts at: 0 358 1089
Size: 358 731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 1089
Size: 543 546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 808 840 1089
Size: 400 32 376 32 249
Fragments arranged by size:
400 376 249 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 214 545 1089
Size: 214 331 544
Fragments arranged by size:
544 331 214
ApaI G_GGCC'C
Cuts at: 0 1078 1089
Size: 1078 11
ApoI r'AATT_y
Cuts at: 0 522 1089
Size: 522 567
BamHI G'GATC_C
Cuts at: 0 752 1089
Size: 752 337
BanI G'GyrC_C
Cuts at: 0 8 1089
Size: 8 1081
BanII G_rGCy'C
Cuts at: 0 992 1063 1078 1089
Size: 992 71 15 11
Fragments arranged by size:
992 71 15 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 966 1000 1089
Size: 966 34 89
Fragments arranged by size:
966 89 34
BclI T'GATC_A
Cuts at: 0 227 923 1089
Size: 227 696 166
Fragments arranged by size:
696 227 166
BglII A'GATC_T
Cuts at: 0 413 1089
Size: 413 676
BlpI GC'TnA_GC
Cuts at: 0 621 1089
Size: 621 468
Bme1580I G_kGCm'C
Cuts at: 0 787 1078 1089
Size: 787 291 11
Fragments arranged by size:
787 291 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 604 657 873 922 1007 1089
Size: 127 477 53 216 49 85 82
Fragments arranged by size:
477 216 127 85 82 53 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1089
Size: 151 938
BsaBI GATnn'nnATC
Cuts at: 0 502 757 1089
Size: 502 255 332
Fragments arranged by size:
502 332 255
BsaWI w'CCGG_w
Cuts at: 0 1045 1089
Size: 1045 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 871 898 901 928 1089
Size: 400 30 441 27 3 27 161
Fragments arranged by size:
441 400 161 30 27 27 3
BseYI C'CCAG_C
Cuts at: 0 736 1089
Size: 736 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 757 1089
Size: 549 208 332
Fragments arranged by size:
549 332 208
BsiHKAI G_wGCw'C
Cuts at: 0 992 1089
Size: 992 97
Bsp1286I G_dGCh'C
Cuts at: 0 787 992 1063 1078 1089
Size: 787 205 71 15 11
Fragments arranged by size:
787 205 71 15 11
BsrBI CCG'CTC
Cuts at: 0 809 1089
Size: 809 280
BsrDI GCAATG_nn'
Cuts at: 0 138 1089
Size: 138 951
BsrFI r'CCGG_y
Cuts at: 0 93 299 662 1089
Size: 93 206 363 427
Fragments arranged by size:
427 363 206 93
BssSI C'ACGA_G
Cuts at: 0 978 1089
Size: 978 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 1089
Size: 15 1074
BstYI r'GATC_y
Cuts at: 0 413 752 1089
Size: 413 339 337
Fragments arranged by size:
413 339 337
BtgI C'CryG_G
Cuts at: 0 872 1054 1089
Size: 872 182 35
Fragments arranged by size:
872 182 35
BtsI GCAGTG_nn'
Cuts at: 0 215 1089
Size: 215 874
DrdI GACnn_nn'nnGTC
Cuts at: 0 76 1089
Size: 76 1013
EaeI y'GGCC_r
Cuts at: 0 707 1037 1089
Size: 707 330 52
Fragments arranged by size:
707 330 52
EarI CTCTTCn'nnn_
Cuts at: 0 316 1089
Size: 316 773
EcoICRI GAG'CTC
Cuts at: 0 990 1089
Size: 990 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 508 522 604 657 873 922 1007
Size: 127 381 14 82 53 216 49 85
Cuts at: 1007 1089
Size: 82
Fragments arranged by size:
381 216 127 85 82 82 53 49
14
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1089
Size: 999 90
HaeII r_GCGC'y
Cuts at: 0 1022 1089
Size: 1022 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 1089
Size: 405 32 652
Fragments arranged by size:
652 405 32
MscI TGG'CCA
Cuts at: 0 709 1039 1089
Size: 709 330 50
Fragments arranged by size:
709 330 50
MslI CAynn'nnrTG
Cuts at: 0 358 700 703 850 1089
Size: 358 342 3 147 239
Fragments arranged by size:
358 342 239 147 3
MspA1I CmG'CkG
Cuts at: 0 932 1089
Size: 932 157
NaeI GCC'GGC
Cuts at: 0 301 1089
Size: 301 788
NcoI C'CATG_G
Cuts at: 0 1054 1089
Size: 1054 35
NgoMIV G'CCGG_C
Cuts at: 0 299 1089
Size: 299 790
NspI r_CATG'y
Cuts at: 0 670 864 1089
Size: 670 194 225
Fragments arranged by size:
670 225 194
PasI CC'CwG_GG
Cuts at: 0 600 1089
Size: 600 489
PfoI T'CCnGG_A
Cuts at: 0 172 581 1089
Size: 172 409 508
Fragments arranged by size:
508 409 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1089
Size: 400 32 657
Fragments arranged by size:
657 400 32
PpuMI rG'GwC_Cy
Cuts at: 0 999 1089
Size: 999 90
PspOMI G'GGCC_C
Cuts at: 0 1074 1089
Size: 1074 15
PstI C_TGCA'G
Cuts at: 0 20 1089
Size: 20 1069
SacI G_AGCT'C
Cuts at: 0 992 1089
Size: 992 97
SanDI GG'GwC_CC
Cuts at: 0 999 1089
Size: 999 90
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1089
Size: 316 773
SfcI C'TryA_G
Cuts at: 0 16 1089
Size: 16 1073
SgrAI Cr'CCGG_yG
Cuts at: 0 93 1089
Size: 93 996
SmlI C'TyrA_G
Cuts at: 0 166 1089
Size: 166 923
SphI G_CATG'C
Cuts at: 0 670 864 1089
Size: 670 194 225
Fragments arranged by size:
670 225 194
StyI C'CwwG_G
Cuts at: 0 1054 1089
Size: 1054 35
TatI w'GTAC_w
Cuts at: 0 878 1089
Size: 878 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1089
Size: 154 32 903
Fragments arranged by size:
903 154 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 579 1089
Size: 579 510
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApaI ApoI
BamHI BanI BanII BcgI BclI BglII BlpI Bme1580I
BpmI BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BsrBI BsrDI BsrFI BssSI BstAPI BstYI BtgI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PfoI PpiI PpuMI PspOMI PstI SacI SanDI
SapI SfcI SgrAI SmlI SphI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbsI BbvCI BciVI BfrBI BglI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI