Restriction map for
pFN21AE0798
(Linear) (Six-base) MAPSORT of: KIEE0798.seq Check: 6044 from: 1 to: 4848
KIEE0798.orf
With 182 enzymes: SgfI *
September 6, 2010 13:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 605 2673 4848
Size: 605 2068 2175
Fragments arranged by size:
2175 2068 605
AatII G_ACGT'C
Cuts at: 0 2093 4344 4493 4848
Size: 2093 2251 149 355
Fragments arranged by size:
2251 2093 355 149
Acc65I G'GTAC_C
Cuts at: 0 188 1610 4848
Size: 188 1422 3238
Fragments arranged by size:
3238 1422 188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1616 4096 4753 4848
Size: 875 741 2480 657 95
Fragments arranged by size:
2480 875 741 657 95
AfeI AGC'GCT
Cuts at: 0 1286 2240 4848
Size: 1286 954 2608
Fragments arranged by size:
2608 1286 954
AflIII A'CryG_T
Cuts at: 0 2996 3149 4848
Size: 2996 153 1699
Fragments arranged by size:
2996 1699 153
AhdI GACnn_n'nnGTC
Cuts at: 0 2232 3708 4848
Size: 2232 1476 1140
Fragments arranged by size:
2232 1476 1140
AleI CACnn'nnGTG
Cuts at: 0 4582 4848
Size: 4582 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1733 2755 4848
Size: 1733 1022 2093
Fragments arranged by size:
2093 1733 1022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3982 4014 4848
Size: 3982 32 834
Fragments arranged by size:
3982 834 32
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1338 1497 3508 3530 3549 4848
Size: 903 435 159 2011 22 19 1299
Fragments arranged by size:
2011 1299 903 435 159 22 19
ApaI G_GGCC'C
Cuts at: 0 58 88 134 323 428 505 773 2414
Size: 58 30 46 189 105 77 268 1641
Cuts at: 2414 2478 4848
Size: 64 2370
Fragments arranged by size:
2370 1641 268 189 105 77 64 58
46 30
ApoI r'AATT_y
Cuts at: 0 1142 3241 3260 4848
Size: 1142 2099 19 1588
Fragments arranged by size:
2099 1588 1142 19
AscI GG'CGCG_CC
Cuts at: 0 3729 4848
Size: 3729 1119
AvaI C'yCGr_G
Cuts at: 0 51 93 631 717 795 808 997 1165
Size: 51 42 538 86 78 13 189 168
Cuts at: 1165 1927 1938 2755 3234 3467 4390 4766 4848
Size: 762 11 817 479 233 923 376 82
Fragments arranged by size:
923 817 762 538 479 376 233 189
168 86 82 78 51 42 13 11
BamHI G'GATC_C
Cuts at: 0 2506 4848
Size: 2506 2342
BanI G'GyrC_C
Cuts at: 0 188 451 732 1026 1610 2706 3091 3786
Size: 188 263 281 294 584 1096 385 695
Cuts at: 3786 4848
Size: 1062
Fragments arranged by size:
1096 1062 695 584 385 294 281 263
188
BanII G_rGCy'C
Cuts at: 0 58 88 112 134 323 428 505 546
Size: 58 30 24 22 189 105 77 41
Cuts at: 546 773 1211 2414 2478 2762 3434 3482 3570
Size: 227 438 1203 64 284 672 48 88
Cuts at: 3570 3929 4406 4537 4848
Size: 359 477 131 311
Fragments arranged by size:
1203 672 477 438 359 311 284 227
189 131 105 88 77 64 58 48
41 30 24 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 428 2359 3785 4350 4556 4566 4848
Size: 410 18 1931 1426 565 206 10 282
Fragments arranged by size:
1931 1426 565 410 282 206 18 10
BbvCI CC'TCA_GC
Cuts at: 0 150 4848
Size: 150 4698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1605 1639 2796 2830 2983 3017 4848
Size: 1605 34 1157 34 153 34 1831
Fragments arranged by size:
1831 1605 1157 153 34 34 34
BclI T'GATC_A
Cuts at: 0 3512 4316 4848
Size: 3512 804 532
Fragments arranged by size:
3512 804 532
BfrBI ATG'CAT
Cuts at: 0 237 4848
Size: 237 4611
BglII A'GATC_T
Cuts at: 0 3131 4848
Size: 3131 1717
BlpI GC'TnA_GC
Cuts at: 0 3460 4348 4848
Size: 3460 888 500
Fragments arranged by size:
3460 888 500
Bme1580I G_kGCm'C
Cuts at: 0 58 88 134 323 428 456 505 773
Size: 58 30 46 189 105 28 49 268
Cuts at: 773 806 2414 2478 2734 3791 4096 4848
Size: 33 1608 64 256 1057 305 752
Fragments arranged by size:
1608 1057 752 305 268 256 189 105
64 58 49 46 33 30 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 984 2460 2952 4848
Size: 984 1476 492 1896
Fragments arranged by size:
1896 1476 984 492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 911 3453 3948 4278 4677 4839 4848
Size: 405 506 2542 495 330 399 162 9
Fragments arranged by size:
2542 506 495 405 399 330 162 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 203 851 1309 1387 1423 1936 3478
Size: 92 111 648 458 78 36 513 1542
Cuts at: 3478 3699 3772 4120 4549 4848
Size: 221 73 348 429 299
Fragments arranged by size:
1542 648 513 458 429 348 299 221
111 92 78 73 36
Bpu10I CC'TnA_GC
Cuts at: 0 150 1307 4824 4848
Size: 150 1157 3517 24
Fragments arranged by size:
3517 1157 150 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 2923 4051 4848
Size: 1195 1728 1128 797
Fragments arranged by size:
1728 1195 1128 797
BsaI GGTCTCn'nnnn_
Cuts at: 0 1816 3793 4848
Size: 1816 1977 1055
Fragments arranged by size:
1977 1816 1055
BsaAI yAC'GTr
Cuts at: 0 1517 1680 1863 2176 2381 4848
Size: 1517 163 183 313 205 2467
Fragments arranged by size:
2467 1517 313 205 183 163
BsaHI Gr'CG_yC
Cuts at: 0 1786 2090 2898 4163 4341 4490 4848
Size: 1786 304 808 1265 178 149 358
Fragments arranged by size:
1786 1265 808 358 304 178 149
BsaWI w'CCGG_w
Cuts at: 0 2543 4848
Size: 2543 2305
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 645 675 1096 1126 3982 4012
Size: 331 30 284 30 421 30 2856 30
Cuts at: 4012 4045 4075 4153 4183 4848
Size: 33 30 78 30 665
Fragments arranged by size:
2856 665 421 331 284 78 33 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 1618 2014 2017 2020 2068 2887 3181
Size: 23 1595 396 3 3 48 819 294
Cuts at: 3181 3646 3775 3787 3844 3847 3892 3931 3994
Size: 465 129 12 57 3 45 39 63
Cuts at: 3994 3997 4000 4003 4102 4117 4129 4161 4639
Size: 3 3 3 99 15 12 32 478
Cuts at: 4639 4648 4651 4666 4816 4828 4848
Size: 9 3 15 150 12 20
Fragments arranged by size:
1595 819 478 465 396 294 150 129
99 63 57 48 45 39 32 23
20 15 15 12 12 12 9 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 81 133 310 328 1010 2511 2537 3472
Size: 81 52 177 18 682 1501 26 935
Cuts at: 3472 4848
Size: 1376
Fragments arranged by size:
1501 1376 935 682 177 81 52 26
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 631 1018 3597 4848
Size: 514 117 387 2579 1251
Fragments arranged by size:
2579 1251 514 387 117
BsiEI CG_ry'CG
Cuts at: 0 625 4005 4246 4743 4753 4781 4848
Size: 625 3380 241 497 10 28 67
Fragments arranged by size:
3380 625 497 241 67 28 10
BsiHKAI G_wGCw'C
Cuts at: 0 546 892 1211 2396 2690 2762 3020 3185
Size: 546 346 319 1185 294 72 258 165
Cuts at: 3185 3605 3758 3929 4406 4848
Size: 420 153 171 477 442
Fragments arranged by size:
1185 546 477 442 420 346 319 294
258 171 165 153 72
BsiWI C'GTAC_G
Cuts at: 0 2456 2576 4848
Size: 2456 120 2272
Fragments arranged by size:
2456 2272 120
BsmI GAATG_Cn'
Cuts at: 0 876 1433 2130 4445 4848
Size: 876 557 697 2315 403
Fragments arranged by size:
2315 876 697 557 403
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2097 2905 4848
Size: 2097 808 1943
Fragments arranged by size:
2097 1943 808
Bsp1286I G_dGCh'C
Cuts at: 0 58 88 112 134 323 428 456 505
Size: 58 30 24 22 189 105 28 49
Cuts at: 505 546 773 806 892 1211 2396 2414 2478
Size: 41 227 33 86 319 1185 18 64
Cuts at: 2478 2690 2734 2762 3020 3185 3434 3482 3570
Size: 212 44 28 258 165 249 48 88
Cuts at: 3570 3605 3758 3791 3929 4096 4406 4537 4848
Size: 35 153 33 138 167 310 131 311
Fragments arranged by size:
1185 319 311 310 258 249 227 212
189 167 165 153 138 131 105 88
86 64 58 49 48 44 41 35
33 33 30 28 28 24 22 18
BspEI T'CCGG_A
Cuts at: 0 2543 4848
Size: 2543 2305
BspHI T'CATG_A
Cuts at: 0 3323 4848
Size: 3323 1525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 605 2673 3557 4848
Size: 605 2068 884 1291
Fragments arranged by size:
2068 1291 884 605
BsrBI CCG'CTC
Cuts at: 0 1536 1560 3726 3966 4823 4848
Size: 1536 24 2166 240 857 25
Fragments arranged by size:
2166 1536 857 240 25 24
BsrFI r'CCGG_y
Cuts at: 0 1005 1403 3691 4165 4727 4742 4776 4848
Size: 1005 398 2288 474 562 15 34 72
Fragments arranged by size:
2288 1005 562 474 398 72 34 15
BsrGI T'GTAC_A
Cuts at: 0 2303 4848
Size: 2303 2545
BssHII G'CGCG_C
Cuts at: 0 3729 4848
Size: 3729 1119
BssSI C'ACGA_G
Cuts at: 0 885 3757 3826 3933 4848
Size: 885 2872 69 107 915
Fragments arranged by size:
2872 915 885 107 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 213 648 1771 2839 3015 4848
Size: 213 435 1123 1068 176 1833
Fragments arranged by size:
1833 1123 1068 435 213 176
BstEII G'GTnAC_C
Cuts at: 0 355 2647 4848
Size: 355 2292 2201
Fragments arranged by size:
2292 2201 355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 336 829 1509 4848
Size: 181 155 493 680 3339
Fragments arranged by size:
3339 680 493 181 155
BstYI r'GATC_y
Cuts at: 0 1094 1471 1877 2073 2506 2615 3131 3638
Size: 1094 377 406 196 433 109 516 507
Cuts at: 3638 4848
Size: 1210
Fragments arranged by size:
1210 1094 516 507 433 406 377 196
109
Bsu36I CC'TnA_GG
Cuts at: 0 194 1219 3370 4848
Size: 194 1025 2151 1478
Fragments arranged by size:
2151 1478 1025 194
BtgI C'CryG_G
Cuts at: 0 224 335 863 1022 1072 2048 2198 2483
Size: 224 111 528 159 50 976 150 285
Cuts at: 2483 4005 4418 4848
Size: 1522 413 430
Fragments arranged by size:
1522 976 528 430 413 285 224 159
150 111 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1116 2099 2352 4848
Size: 1116 983 253 2496
Fragments arranged by size:
2496 1116 983 253
BtsI GCAGTG_nn'
Cuts at: 0 1030 1514 2667 2827 4066 4341 4848
Size: 1030 484 1153 160 1239 275 507
Fragments arranged by size:
1239 1153 1030 507 484 275 160
DraI TTT'AAA
Cuts at: 0 3363 4848
Size: 3363 1485
DraIII CAC_nnn'GTG
Cuts at: 0 2703 2910 3294 4848
Size: 2703 207 384 1554
Fragments arranged by size:
2703 1554 384 207
DrdI GACnn_nn'nnGTC
Cuts at: 0 3802 4558 4848
Size: 3802 756 290
Fragments arranged by size:
3802 756 290
EaeI y'GGCC_r
Cuts at: 0 37 569 622 1175 1511 2330 4002 4243
Size: 37 532 53 553 336 819 1672 241
Cuts at: 4243 4704 4740 4750 4848
Size: 461 36 10 98
Fragments arranged by size:
1672 819 553 532 461 336 241 98
53 37 36 10
EagI C'GGCC_G
Cuts at: 0 622 4002 4243 4740 4750 4848
Size: 622 3380 241 497 10 98
Fragments arranged by size:
3380 622 497 241 98 10
EarI CTCTTCn'nnn_
Cuts at: 0 1985 1991 2006 4106 4637 4848
Size: 1985 6 15 2100 531 211
Fragments arranged by size:
2100 1985 531 211 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 41 102 3409 3595 4848
Size: 41 61 3307 186 1253
Fragments arranged by size:
3307 1253 186 61 41
EcoICRI GAG'CTC
Cuts at: 0 544 1209 2760 3927 4404 4848
Size: 544 665 1551 1167 477 444
Fragments arranged by size:
1551 1167 665 544 477 444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 203 851 875 1309 1387 1423 1616
Size: 92 111 648 24 434 78 36 193
Cuts at: 1616 1936 3478 3699 3772 4096 4120 4549 4753
Size: 320 1542 221 73 324 24 429 204
Cuts at: 4753 4848
Size: 95
Fragments arranged by size:
1542 648 434 429 324 320 221 204
193 111 95 92 78 73 36 24
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 38 785 830 1342 2869 3913 4483 4848
Size: 38 747 45 512 1527 1044 570 365
Fragments arranged by size:
1527 1044 747 570 512 365 45 38
EcoO109I rG'GnC_Cy
Cuts at: 0 43 55 85 97 130 178 319 320
Size: 43 12 30 12 33 48 141 1
Cuts at: 320 424 425 501 685 721 769 790 835
Size: 104 1 76 184 36 48 21 45
Cuts at: 835 859 1077 1169 1215 2166 2411 2475 3546
Size: 24 218 92 46 951 245 64 1071
Cuts at: 3546 4763 4848
Size: 1217 85
Fragments arranged by size:
1217 1071 951 245 218 184 141 104
92 85 76 64 48 48 46 45
43 36 33 30 24 21 12 12
1 1
EcoRI G'AATT_C
Cuts at: 0 1142 3260 4848
Size: 1142 2118 1588
Fragments arranged by size:
2118 1588 1142
FspI TGC'GCA
Cuts at: 0 3853 4848
Size: 3853 995
FspAI rTGC'GCAy
Cuts at: 0 3853 4848
Size: 3853 995
HaeII r_GCGC'y
Cuts at: 0 1288 1332 1404 1647 2242 2770 4848
Size: 1288 44 72 243 595 528 2078
Fragments arranged by size:
2078 1288 595 528 243 72 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 331 363 533 565 2058 2077
Size: 149 32 150 32 170 32 1493 19
Cuts at: 2077 2090 2109 2218 2250 3694 3726 4153 4185
Size: 13 19 109 32 1444 32 427 32
Cuts at: 4185 4331 4363 4848
Size: 146 32 485
Fragments arranged by size:
1493 1444 485 427 170 150 149 146
109 32 32 32 32 32 32 32
19 19 13
HincII GTy'rAC
Cuts at: 0 3621 3807 4848
Size: 3621 186 1041
Fragments arranged by size:
3621 1041 186
HindIII A'AGCT_T
Cuts at: 0 2749 4699 4848
Size: 2749 1950 149
Fragments arranged by size:
2749 1950 149
KpnI G_GTAC'C
Cuts at: 0 192 1614 4848
Size: 192 1422 3234
Fragments arranged by size:
3234 1422 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 2471 2602 2955 4848
Size: 233 2238 131 353 1893
Fragments arranged by size:
2238 1893 353 233 131
MscI TGG'CCA
Cuts at: 0 571 1513 4848
Size: 571 942 3335
Fragments arranged by size:
3335 942 571
MslI CAynn'nnrTG
Cuts at: 0 2425 2635 3115 3853 4582 4848
Size: 2425 210 480 738 729 266
Fragments arranged by size:
2425 738 729 480 266 210
MspA1I CmG'CkG
Cuts at: 0 459 528 1074 1245 1914 2739 3123 4368
Size: 459 69 546 171 669 825 384 1245
Cuts at: 4368 4848
Size: 480
Fragments arranged by size:
1245 825 669 546 480 459 384 171
69
NaeI GCC'GGC
Cuts at: 0 1007 1405 3693 4167 4744 4848
Size: 1007 398 2288 474 577 104
Fragments arranged by size:
2288 1007 577 474 398 104
NcoI C'CATG_G
Cuts at: 0 224 335 863 1022 4848
Size: 224 111 528 159 3826
Fragments arranged by size:
3826 528 224 159 111
NgoMIV G'CCGG_C
Cuts at: 0 1005 1403 3691 4165 4742 4848
Size: 1005 398 2288 474 577 106
Fragments arranged by size:
2288 1005 577 474 398 106
NotI GC'GGCC_GC
Cuts at: 0 4243 4848
Size: 4243 605
NruI TCG'CGA
Cuts at: 0 4377 4848
Size: 4377 471
NsiI A_TGCA'T
Cuts at: 0 239 4848
Size: 239 4609
NspI r_CATG'y
Cuts at: 0 207 252 388 780 1272 1447 1581 3000
Size: 207 45 136 392 492 175 134 1419
Cuts at: 3000 3033 3153 3411 3699 4848
Size: 33 120 258 288 1149
Fragments arranged by size:
1419 1149 492 392 288 258 207 175
136 134 120 45 33
PasI CC'CwG_GG
Cuts at: 0 21 139 174 369 4848
Size: 21 118 35 195 4479
Fragments arranged by size:
4479 195 118 35 21
PciI A'CATG_T
Cuts at: 0 2996 3149 4848
Size: 2996 153 1699
Fragments arranged by size:
2996 1699 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 409 483 848 1257 3216 4848
Size: 409 74 365 409 1959 1632
Fragments arranged by size:
1959 1632 409 409 365 74
PfoI T'CCnGG_A
Cuts at: 0 828 3749 4848
Size: 828 2921 1099
Fragments arranged by size:
2921 1099 828
PmlI CAC'GTG
Cuts at: 0 1863 2176 4848
Size: 1863 313 2672
Fragments arranged by size:
2672 1863 313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3982 4014 4848
Size: 3982 32 834
Fragments arranged by size:
3982 834 32
PpuMI rG'GwC_Cy
Cuts at: 0 43 97 685 721 790 859 1169 3546
Size: 43 54 588 36 69 69 310 2377
Cuts at: 3546 4763 4848
Size: 1217 85
Fragments arranged by size:
2377 1217 588 310 85 69 69 54
43 36
PshAI GACnn'nnGTC
Cuts at: 0 2645 4848
Size: 2645 2203
PspOMI G'GGCC_C
Cuts at: 0 54 84 130 319 424 501 769 2410
Size: 54 30 46 189 105 77 268 1641
Cuts at: 2410 2474 4848
Size: 64 2374
Fragments arranged by size:
2374 1641 268 189 105 77 64 54
46 30
PstI C_TGCA'G
Cuts at: 0 602 1103 1124 2186 2744 2809 3011 3651
Size: 602 501 21 1062 558 65 202 640
Cuts at: 3651 3743 4848
Size: 92 1105
Fragments arranged by size:
1105 1062 640 602 558 501 202 92
65 21
PvuII CAG'CTG
Cuts at: 0 528 1245 1914 3123 4368 4848
Size: 528 717 669 1209 1245 480
Fragments arranged by size:
1245 1209 717 669 528 480
RsrII CG'GwC_CG
Cuts at: 0 4679 4848
Size: 4679 169
SacI G_AGCT'C
Cuts at: 0 546 1211 2762 3929 4406 4848
Size: 546 665 1551 1167 477 442
Fragments arranged by size:
1551 1167 665 546 477 442
SacII CC_GC'GG
Cuts at: 0 1075 4848
Size: 1075 3773
SanDI GG'GwC_CC
Cuts at: 0 97 721 1169 4763 4848
Size: 97 624 448 3594 85
Fragments arranged by size:
3594 624 448 97 85
SapI GCTCTTCn'nnn_
Cuts at: 0 2006 4848
Size: 2006 2842
SbfI CC_TGCA'GG
Cuts at: 0 1103 3743 4848
Size: 1103 2640 1105
Fragments arranged by size:
2640 1105 1103
ScaI AGT'ACT
Cuts at: 0 3445 4396 4848
Size: 3445 951 452
Fragments arranged by size:
3445 951 452
SfcI C'TryA_G
Cuts at: 0 598 1099 1120 2182 2740 2805 3007 3647
Size: 598 501 21 1062 558 65 202 640
Cuts at: 3647 3739 4035 4848
Size: 92 296 813
Fragments arranged by size:
1062 813 640 598 558 501 296 202
92 65 21
SmaI CCC'GGG
Cuts at: 0 53 95 633 719 810 999 1167 1940
Size: 53 42 538 86 91 189 168 773
Cuts at: 1940 3469 4848
Size: 1529 1379
Fragments arranged by size:
1529 1379 773 538 189 168 91 86
53 42
SmlI C'TyrA_G
Cuts at: 0 1210 1927 2938 4066 4848
Size: 1210 717 1011 1128 782
Fragments arranged by size:
1210 1128 1011 782 717
SphI G_CATG'C
Cuts at: 0 388 1272 1447 1581 3411 4848
Size: 388 884 175 134 1830 1437
Fragments arranged by size:
1830 1437 884 388 175 134
StuI AGG'CCT
Cuts at: 0 481 753 1312 1921 4848
Size: 481 272 559 609 2927
Fragments arranged by size:
2927 609 559 481 272
StyI C'CwwG_G
Cuts at: 0 224 335 351 482 863 1022 1448 1502
Size: 224 111 16 131 381 159 426 54
Cuts at: 1502 1730 3038 3210 4848
Size: 228 1308 172 1638
Fragments arranged by size:
1638 1308 426 381 228 224 172 159
131 111 54 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 361 3578 4290 4560 4667 4848
Size: 187 174 3217 712 270 107 181
Fragments arranged by size:
3217 712 270 187 181 174 107
TatI w'GTAC_w
Cuts at: 0 1112 2303 2582 2627 3443 3656 4322 4394
Size: 1112 1191 279 45 816 213 666 72
Cuts at: 4394 4848
Size: 454
Fragments arranged by size:
1191 1112 816 666 454 279 213 72
45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4045 4077 4848
Size: 4045 32 771
Fragments arranged by size:
4045 771 32
Tth111I GACn'n_nGTC
Cuts at: 0 990 4848
Size: 990 3858
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1867 2046 2545 4848
Size: 1867 179 499 2303
Fragments arranged by size:
2303 1867 499 179
XhoI C'TCGA_G
Cuts at: 0 1927 4848
Size: 1927 2921
XmaI C'CCGG_G
Cuts at: 0 51 93 631 717 808 997 1165 1938
Size: 51 42 538 86 91 189 168 773
Cuts at: 1938 3467 4848
Size: 1529 1381
Fragments arranged by size:
1529 1381 773 538 189 168 91 86
51 42
XmnI GAAnn'nnTTC
Cuts at: 0 1160 2882 3421 3997 4848
Size: 1160 1722 539 576 851
Fragments arranged by size:
1722 1160 851 576 539
ZraI GAC'GTC
Cuts at: 0 2091 4342 4491 4848
Size: 2091 2251 149 357
Fragments arranged by size:
2251 2091 357 149
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApoI AscI AvaI BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI ApaLI AseI AsiSI AvrII
BaeI BbeI BciVI BglI BmgBI BmtI BsaBI BsrDI
BstBI BstZ17I ClaI CspCI EcoRV FalI FseI HpaI
KasI MfeI MluI NarI NdeI NheI PacI PmeI
PsiI PspXI PsrI PvuI SalI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI