Restriction map for
pFN21AE0744
(Linear) (Six-base) MAPSORT of: KIEE0744.seq Check: 6190 from: 1 to: 1272
KIEE0744.orf
With 182 enzymes: SgfI *
March 22, 2012 15:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 1227 1272
Size: 402 825 45
Fragments arranged by size:
825 402 45
AatII G_ACGT'C
Cuts at: 0 146 1272
Size: 146 1126
AccI GT'mk_AC
Cuts at: 0 212 1272
Size: 212 1060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 262 292 1272
Size: 119 143 30 980
Fragments arranged by size:
980 143 119 30
AfeI AGC'GCT
Cuts at: 0 457 880 1272
Size: 457 423 392
Fragments arranged by size:
457 423 392
AflIII A'CryG_T
Cuts at: 0 704 1272
Size: 704 568
AleI CACnn'nnGTG
Cuts at: 0 697 1272
Size: 697 575
AlwNI CAG_nnn'CTG
Cuts at: 0 22 114 471 1272
Size: 22 92 357 801
Fragments arranged by size:
801 357 92 22
ApaI G_GGCC'C
Cuts at: 0 1196 1272
Size: 1196 76
ApaLI G'TGCA_C
Cuts at: 0 511 1272
Size: 511 761
AvaI C'yCGr_G
Cuts at: 0 529 898 997 1033 1168 1272
Size: 529 369 99 36 135 104
Fragments arranged by size:
529 369 135 104 99 36
BanI G'GyrC_C
Cuts at: 0 76 184 628 645 772 842 1038 1116
Size: 76 108 444 17 127 70 196 78
Cuts at: 1116 1134 1140 1158 1272
Size: 18 6 18 114
Fragments arranged by size:
444 196 127 114 108 78 76 70
18 18 17 6
BanII G_rGCy'C
Cuts at: 0 437 860 899 950 1175 1196 1272
Size: 437 423 39 51 225 21 76
Fragments arranged by size:
437 423 225 76 51 39 21
BbeI G_GCGC'C
Cuts at: 0 649 776 846 1042 1272
Size: 649 127 70 196 230
Fragments arranged by size:
649 230 196 127 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 1272
Size: 107 1165
BglI GCCn_nnn'nGGC
Cuts at: 0 602 665 1272
Size: 602 63 607
Fragments arranged by size:
607 602 63
BglII A'GATC_T
Cuts at: 0 1262 1272
Size: 1262 10
Bme1580I G_kGCm'C
Cuts at: 0 89 223 515 1121 1151 1169 1196 1272
Size: 89 134 292 606 30 18 27 76
Fragments arranged by size:
606 292 134 89 76 30 27 18
BmgBI CAC'GTC
Cuts at: 0 345 705 953 1272
Size: 345 360 248 319
Fragments arranged by size:
360 345 319 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 913 1272
Size: 300 613 359
Fragments arranged by size:
613 359 300
Bpu10I CC'TnA_GC
Cuts at: 0 23 1272
Size: 23 1249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1036 1272
Size: 922 114 236
Fragments arranged by size:
922 236 114
BsaHI Gr'CG_yC
Cuts at: 0 143 646 766 773 779 843 1039 1272
Size: 143 503 120 7 6 64 196 233
Fragments arranged by size:
503 233 196 143 120 64 7 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 751 781 1272
Size: 295 30 426 30 491
Fragments arranged by size:
491 426 295 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 303 805 1272
Size: 303 502 467
Fragments arranged by size:
502 467 303
BseYI C'CCAG_C
Cuts at: 0 174 397 471 912 1272
Size: 174 223 74 441 360
Fragments arranged by size:
441 360 223 174 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1272
Size: 484 788
BsiEI CG_ry'CG
Cuts at: 0 552 1203 1215 1272
Size: 552 651 12 57
Fragments arranged by size:
651 552 57 12
BsiHKAI G_wGCw'C
Cuts at: 0 515 725 740 950 1272
Size: 515 210 15 210 322
Fragments arranged by size:
515 322 210 210 15
Bsp1286I G_dGCh'C
Cuts at: 0 89 223 437 515 725 740 860 899
Size: 89 134 214 78 210 15 120 39
Cuts at: 899 950 1121 1151 1169 1175 1196 1272
Size: 51 171 30 18 6 21 76
Fragments arranged by size:
214 210 171 134 120 89 78 76
51 39 30 21 18 15 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 402 1227 1272
Size: 52 350 825 45
Fragments arranged by size:
825 350 52 45
BsrBI CCG'CTC
Cuts at: 0 35 626 1050 1076 1272
Size: 35 591 424 26 196
Fragments arranged by size:
591 424 196 35 26
BsrFI r'CCGG_y
Cuts at: 0 79 742 968 1131 1137 1155 1272
Size: 79 663 226 163 6 18 117
Fragments arranged by size:
663 226 163 117 79 18 6
BssHII G'CGCG_C
Cuts at: 0 983 985 1272
Size: 983 2 287
Fragments arranged by size:
983 287 2
BssSI C'ACGA_G
Cuts at: 0 943 1272
Size: 943 329
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 1242 1272
Size: 510 732 30
Fragments arranged by size:
732 510 30
BstYI r'GATC_y
Cuts at: 0 1262 1272
Size: 1262 10
Bsu36I CC'TnA_GG
Cuts at: 0 440 650 1272
Size: 440 210 622
Fragments arranged by size:
622 440 210
BtgI C'CryG_G
Cuts at: 0 754 906 1250 1272
Size: 754 152 344 22
Fragments arranged by size:
754 344 152 22
BtsI GCAGTG_nn'
Cuts at: 0 844 1024 1272
Size: 844 180 248
Fragments arranged by size:
844 248 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 208 370 1272
Size: 208 162 902
Fragments arranged by size:
902 208 162
EaeI y'GGCC_r
Cuts at: 0 131 575 1200 1212 1247 1253 1272
Size: 131 444 625 12 35 6 19
Fragments arranged by size:
625 444 131 35 19 12 6
EagI C'GGCC_G
Cuts at: 0 1200 1212 1272
Size: 1200 12 60
Fragments arranged by size:
1200 60 12
EcoICRI GAG'CTC
Cuts at: 0 948 1272
Size: 948 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 262 292 300 913 1272
Size: 119 143 30 8 613 359
Fragments arranged by size:
613 359 143 119 30 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 286 1272
Size: 286 986
EcoO109I rG'GnC_Cy
Cuts at: 0 111 402 1192 1193 1272
Size: 111 291 790 1 79
Fragments arranged by size:
790 291 111 79 1
FspI TGC'GCA
Cuts at: 0 868 1272
Size: 868 404
HaeII r_GCGC'y
Cuts at: 0 459 484 649 776 846 882 906 1042
Size: 459 25 165 127 70 36 24 136
Cuts at: 1042 1272
Size: 230
Fragments arranged by size:
459 230 165 136 127 70 36 25
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 937 969 1272
Size: 751 32 154 32 303
Fragments arranged by size:
751 303 154 32 32
HincII GTy'rAC
Cuts at: 0 201 1272
Size: 201 1071
KasI G'GCGC_C
Cuts at: 0 645 772 842 1038 1272
Size: 645 127 70 196 234
Fragments arranged by size:
645 234 196 127 70
MscI TGG'CCA
Cuts at: 0 1249 1272
Size: 1249 23
MslI CAynn'nnrTG
Cuts at: 0 232 697 1272
Size: 232 465 575
Fragments arranged by size:
575 465 232
MspA1I CmG'CkG
Cuts at: 0 471 507 810 908 1082 1272
Size: 471 36 303 98 174 190
Fragments arranged by size:
471 303 190 174 98 36
NaeI GCC'GGC
Cuts at: 0 970 1272
Size: 970 302
NarI GG'CG_CC
Cuts at: 0 646 773 843 1039 1272
Size: 646 127 70 196 233
Fragments arranged by size:
646 233 196 127 70
NgoMIV G'CCGG_C
Cuts at: 0 968 1272
Size: 968 304
NspI r_CATG'y
Cuts at: 0 108 873 1272
Size: 108 765 399
Fragments arranged by size:
765 399 108
PasI CC'CwG_GG
Cuts at: 0 67 1188 1272
Size: 67 1121 84
Fragments arranged by size:
1121 84 67
PfoI T'CCnGG_A
Cuts at: 0 167 1272
Size: 167 1105
PpuMI rG'GwC_Cy
Cuts at: 0 402 1272
Size: 402 870
PspOMI G'GGCC_C
Cuts at: 0 1192 1272
Size: 1192 80
PspXI vC'TCGA_Gb
Cuts at: 0 1033 1272
Size: 1033 239
PvuII CAG'CTG
Cuts at: 0 471 507 1272
Size: 471 36 765
Fragments arranged by size:
765 471 36
RsrII CG'GwC_CG
Cuts at: 0 1205 1272
Size: 1205 67
SacI G_AGCT'C
Cuts at: 0 950 1272
Size: 950 322
SacII CC_GC'GG
Cuts at: 0 909 1272
Size: 909 363
SexAI A'CCwGG_T
Cuts at: 0 203 1272
Size: 203 1069
SfoI GGC'GCC
Cuts at: 0 647 774 844 1040 1272
Size: 647 127 70 196 232
Fragments arranged by size:
647 232 196 127 70
SmaI CCC'GGG
Cuts at: 0 999 1272
Size: 999 273
SmlI C'TyrA_G
Cuts at: 0 937 1033 1051 1272
Size: 937 96 18 221
Fragments arranged by size:
937 221 96 18
SpeI A'CTAG_T
Cuts at: 0 162 1272
Size: 162 1110
StyI C'CwwG_G
Cuts at: 0 917 1272
Size: 917 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1084 1222 1272
Size: 1084 138 50
Fragments arranged by size:
1084 138 50
Tth111I GACn'n_nGTC
Cuts at: 0 346 1272
Size: 346 926
XhoI C'TCGA_G
Cuts at: 0 1033 1272
Size: 1033 239
XmaI C'CCGG_G
Cuts at: 0 997 1272
Size: 997 275
XmnI GAAnn'nnTTC
Cuts at: 0 277 1272
Size: 277 995
ZraI GAC'GTC
Cuts at: 0 144 1272
Size: 144 1128
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtsI
DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PfoI PpuMI PspOMI PspXI
PvuII RsrII SacI SacII SexAI SfoI SmaI SmlI
SpeI StyI TaqII Tth111I XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII EarI EciI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI
PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
TatI TstI XbaI XcmI