Restriction map for
pFN21AE0732
(Linear) (Six-base) MAPSORT of: KIEE0732.seq Check: 8659 from: 1 to: 1260
KIEE0732.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AccI GT'mk_AC
Cuts at: 0 524 1260
Size: 524 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1260
Size: 755 505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 353 1260
Size: 353 907
AlwNI CAG_nnn'CTG
Cuts at: 0 553 1260
Size: 553 707
ApaI G_GGCC'C
Cuts at: 0 171 1260
Size: 171 1089
ApoI r'AATT_y
Cuts at: 0 391 1260
Size: 391 869
AscI GG'CGCG_CC
Cuts at: 0 160 1260
Size: 160 1100
AvaI C'yCGr_G
Cuts at: 0 1220 1260
Size: 1220 40
BamHI G'GATC_C
Cuts at: 0 403 1260
Size: 403 857
BanI G'GyrC_C
Cuts at: 0 242 1260
Size: 242 1018
BanII G_rGCy'C
Cuts at: 0 171 724 1260
Size: 171 553 536
Fragments arranged by size:
553 536 171
BbeI G_GCGC'C
Cuts at: 0 246 1260
Size: 246 1014
BciVI GTATCCnnnnn_n'
Cuts at: 0 465 720 792 1260
Size: 465 255 72 468
Fragments arranged by size:
468 465 255 72
BclI T'GATC_A
Cuts at: 0 1062 1101 1260
Size: 1062 39 159
Fragments arranged by size:
1062 159 39
BglI GCCn_nnn'nGGC
Cuts at: 0 105 1260
Size: 105 1155
BglII A'GATC_T
Cuts at: 0 486 1260
Size: 486 774
Bme1580I G_kGCm'C
Cuts at: 0 171 1260
Size: 171 1089
BmgBI CAC'GTC
Cuts at: 0 180 1260
Size: 180 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 563 1148 1260
Size: 563 585 112
Fragments arranged by size:
585 563 112
BmtI G_CTAG'C
Cuts at: 0 115 1260
Size: 115 1145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 1260
Size: 483 777
BsaHI Gr'CG_yC
Cuts at: 0 243 1260
Size: 243 1017
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 1260
Size: 112 1148
BseYI C'CCAG_C
Cuts at: 0 139 170 188 467 1260
Size: 139 31 18 279 793
Fragments arranged by size:
793 279 139 31 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 1260
Size: 513 747
BsiEI CG_ry'CG
Cuts at: 0 94 100 1260
Size: 94 6 1160
Fragments arranged by size:
1160 94 6
BsiHKAI G_wGCw'C
Cuts at: 0 1204 1260
Size: 1204 56
Bsp1286I G_dGCh'C
Cuts at: 0 171 724 1204 1260
Size: 171 553 480 56
Fragments arranged by size:
553 480 171 56
BsrBI CCG'CTC
Cuts at: 0 52 120 1260
Size: 52 68 1140
Fragments arranged by size:
1140 68 52
BssHII G'CGCG_C
Cuts at: 0 160 322 1260
Size: 160 162 938
Fragments arranged by size:
938 162 160
BstXI CCAn_nnnn'nTGG
Cuts at: 0 892 1260
Size: 892 368
BstYI r'GATC_y
Cuts at: 0 249 403 486 543 654 1260
Size: 249 154 83 57 111 606
Fragments arranged by size:
606 249 154 111 83 57
BtgI C'CryG_G
Cuts at: 0 94 103 794 1260
Size: 94 9 691 466
Fragments arranged by size:
691 466 94 9
DraI TTT'AAA
Cuts at: 0 270 677 1260
Size: 270 407 583
Fragments arranged by size:
583 407 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 349 1260
Size: 349 911
EaeI y'GGCC_r
Cuts at: 0 91 97 1014 1191 1260
Size: 91 6 917 177 69
Fragments arranged by size:
917 177 91 69 6
EagI C'GGCC_G
Cuts at: 0 91 97 1260
Size: 91 6 1163
Fragments arranged by size:
1163 91 6
EarI CTCTTCn'nnn_
Cuts at: 0 259 813 845 966 1260
Size: 259 554 32 121 294
Fragments arranged by size:
554 294 259 121 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 135 247 282 1139 1260
Size: 135 112 35 857 121
Fragments arranged by size:
857 135 121 112 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1260
Size: 755 505
EcoO109I rG'GnC_Cy
Cuts at: 0 6 1260
Size: 6 1254
EcoRI G'AATT_C
Cuts at: 0 391 1260
Size: 391 869
HaeII r_GCGC'y
Cuts at: 0 177 246 1260
Size: 177 69 1014
Fragments arranged by size:
1014 177 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 411 427 443 487 519 1260
Size: 395 16 16 16 44 32 741
Fragments arranged by size:
741 395 44 32 16 16 16
HindIII A'AGCT_T
Cuts at: 0 671 1260
Size: 671 589
KasI G'GCGC_C
Cuts at: 0 242 1260
Size: 242 1018
MscI TGG'CCA
Cuts at: 0 1193 1260
Size: 1193 67
MspA1I CmG'CkG
Cuts at: 0 96 105 301 985 1260
Size: 96 9 196 684 275
Fragments arranged by size:
684 275 196 96 9
NarI GG'CG_CC
Cuts at: 0 243 1260
Size: 243 1017
NcoI C'CATG_G
Cuts at: 0 794 1260
Size: 794 466
NheI G'CTAG_C
Cuts at: 0 111 1260
Size: 111 1149
NotI GC'GGCC_GC
Cuts at: 0 91 97 1260
Size: 91 6 1163
Fragments arranged by size:
1163 91 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 1260
Size: 553 707
PpuMI rG'GwC_Cy
Cuts at: 0 6 1260
Size: 6 1254
PspOMI G'GGCC_C
Cuts at: 0 167 1260
Size: 167 1093
PstI C_TGCA'G
Cuts at: 0 984 1260
Size: 984 276
PvuII CAG'CTG
Cuts at: 0 985 1260
Size: 985 275
SacII CC_GC'GG
Cuts at: 0 97 106 1260
Size: 97 9 1154
Fragments arranged by size:
1154 97 9
SapI GCTCTTCn'nnn_
Cuts at: 0 845 1260
Size: 845 415
ScaI AGT'ACT
Cuts at: 0 499 1260
Size: 499 761
SfcI C'TryA_G
Cuts at: 0 980 1110 1185 1260
Size: 980 130 75 75
Fragments arranged by size:
980 130 75 75
SfoI GGC'GCC
Cuts at: 0 244 1260
Size: 244 1016
SmlI C'TyrA_G
Cuts at: 0 462 1220 1260
Size: 462 758 40
Fragments arranged by size:
758 462 40
SspI AAT'ATT
Cuts at: 0 754 783 1260
Size: 754 29 477
Fragments arranged by size:
754 477 29
StyI C'CwwG_G
Cuts at: 0 794 1041 1260
Size: 794 247 219
Fragments arranged by size:
794 247 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 1260
Size: 700 560
TatI w'GTAC_w
Cuts at: 0 374 497 1260
Size: 374 123 763
Fragments arranged by size:
763 374 123
XhoI C'TCGA_G
Cuts at: 0 1220 1260
Size: 1220 40
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApoI AscI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BpuEI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrBI BssHII BstXI BstYI
BtgI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI HaeII Hin4I HindIII KasI MscI MspA1I
NarI NcoI NheI NotI PflMI PpuMI PspOMI PstI
PvuII SacII SapI ScaI SfcI SfoI SmlI SspI
StyI TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BfrBI BlpI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI MslI NaeI
NdeI NgoMIV NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XmaI XmnI ZraI