Restriction map for
pFN21AE0718
(Linear) (Six-base) MAPSORT of: KIEE0718.seq Check: 1626 from: 1 to: 1242
KIEE0718.orf
With 182 enzymes: SgfI *
March 22, 2012 15:47 ..
Acc65I G'GTAC_C
Cuts at: 0 673 1242
Size: 673 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 956 1242
Size: 938 18 286
Fragments arranged by size:
938 286 18
AleI CACnn'nnGTG
Cuts at: 0 536 1242
Size: 536 706
ApoI r'AATT_y
Cuts at: 0 211 256 698 834 856 1195 1220 1231
Size: 211 45 442 136 22 339 25 11
Cuts at: 1231 1242
Size: 11
Fragments arranged by size:
442 339 211 136 45 25 22 11
11
AseI AT'TA_AT
Cuts at: 0 365 1055 1242
Size: 365 690 187
Fragments arranged by size:
690 365 187
AvaI C'yCGr_G
Cuts at: 0 57 186 1242
Size: 57 129 1056
Fragments arranged by size:
1056 129 57
BanI G'GyrC_C
Cuts at: 0 43 673 1242
Size: 43 630 569
Fragments arranged by size:
630 569 43
BanII G_rGCy'C
Cuts at: 0 161 659 1242
Size: 161 498 583
Fragments arranged by size:
583 498 161
BbeI G_GCGC'C
Cuts at: 0 47 1242
Size: 47 1195
BciVI GTATCCnnnnn_n'
Cuts at: 0 1056 1242
Size: 1056 186
BglI GCCn_nnn'nGGC
Cuts at: 0 68 93 1242
Size: 68 25 1149
Fragments arranged by size:
1149 68 25
Bme1580I G_kGCm'C
Cuts at: 0 187 1242
Size: 187 1055
BsaHI Gr'CG_yC
Cuts at: 0 44 1242
Size: 44 1198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 1242
Size: 24 1218
BseYI C'CCAG_C
Cuts at: 0 198 424 1242
Size: 198 226 818
Fragments arranged by size:
818 226 198
BsiEI CG_ry'CG
Cuts at: 0 97 1242
Size: 97 1145
BsiHKAI G_wGCw'C
Cuts at: 0 659 1242
Size: 659 583
Bsp1286I G_dGCh'C
Cuts at: 0 161 187 659 1242
Size: 161 26 472 583
Fragments arranged by size:
583 472 161 26
BspHI T'CATG_A
Cuts at: 0 272 318 1242
Size: 272 46 924
Fragments arranged by size:
924 272 46
BsrBI CCG'CTC
Cuts at: 0 83 1242
Size: 83 1159
BsrDI GCAATG_nn'
Cuts at: 0 801 1242
Size: 801 441
BsrFI r'CCGG_y
Cuts at: 0 92 1242
Size: 92 1150
BssSI C'ACGA_G
Cuts at: 0 536 1242
Size: 536 706
Bsu36I CC'TnA_GG
Cuts at: 0 51 1242
Size: 51 1191
BtgI C'CryG_G
Cuts at: 0 117 143 1242
Size: 117 26 1099
Fragments arranged by size:
1099 117 26
BtsI GCAGTG_nn'
Cuts at: 0 224 929 1242
Size: 224 705 313
Fragments arranged by size:
705 313 224
DraI TTT'AAA
Cuts at: 0 474 1194 1242
Size: 474 720 48
Fragments arranged by size:
720 474 48
EaeI y'GGCC_r
Cuts at: 0 94 1242
Size: 94 1148
EagI C'GGCC_G
Cuts at: 0 94 1242
Size: 94 1148
EarI CTCTTCn'nnn_
Cuts at: 0 647 1097 1242
Size: 647 450 145
Fragments arranged by size:
647 450 145
EcoICRI GAG'CTC
Cuts at: 0 657 1242
Size: 657 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 956 1242
Size: 938 18 286
Fragments arranged by size:
938 286 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 788 1242
Size: 788 454
FspI TGC'GCA
Cuts at: 0 151 1242
Size: 151 1091
FspAI rTGC'GCAy
Cuts at: 0 151 1242
Size: 151 1091
HaeII r_GCGC'y
Cuts at: 0 47 1242
Size: 47 1195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 853 885 1220 1242
Size: 376 32 445 32 335 22
Fragments arranged by size:
445 376 335 32 32 22
HincII GTy'rAC
Cuts at: 0 128 581 755 962 1242
Size: 128 453 174 207 280
Fragments arranged by size:
453 280 207 174 128
HpaI GTT'AAC
Cuts at: 0 755 1242
Size: 755 487
KasI G'GCGC_C
Cuts at: 0 43 1242
Size: 43 1199
KpnI G_GTAC'C
Cuts at: 0 677 1242
Size: 677 565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1242
Size: 283 959
MslI CAynn'nnrTG
Cuts at: 0 536 748 1242
Size: 536 212 494
Fragments arranged by size:
536 494 212
MspA1I CmG'CkG
Cuts at: 0 26 119 1242
Size: 26 93 1123
Fragments arranged by size:
1123 93 26
NaeI GCC'GGC
Cuts at: 0 94 1242
Size: 94 1148
NarI GG'CG_CC
Cuts at: 0 44 1242
Size: 44 1198
NdeI CA'TA_TG
Cuts at: 0 759 1242
Size: 759 483
NgoMIV G'CCGG_C
Cuts at: 0 92 1242
Size: 92 1150
PfoI T'CCnGG_A
Cuts at: 0 164 1224 1242
Size: 164 1060 18
Fragments arranged by size:
1060 164 18
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1196 1228 1242
Size: 1196 32 14
Fragments arranged by size:
1196 32 14
SacI G_AGCT'C
Cuts at: 0 659 1242
Size: 659 583
SacII CC_GC'GG
Cuts at: 0 120 1242
Size: 120 1122
SapI GCTCTTCn'nnn_
Cuts at: 0 647 1242
Size: 647 595
SfcI C'TryA_G
Cuts at: 0 174 1242
Size: 174 1068
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 68 93 1242
Size: 68 25 1149
Fragments arranged by size:
1149 68 25
SfoI GGC'GCC
Cuts at: 0 45 1242
Size: 45 1197
SmaI CCC'GGG
Cuts at: 0 188 1242
Size: 188 1054
SrfI GCCC'GGGC
Cuts at: 0 188 1242
Size: 188 1054
SspI AAT'ATT
Cuts at: 0 573 638 1242
Size: 573 65 604
Fragments arranged by size:
604 573 65
StyI C'CwwG_G
Cuts at: 0 355 1242
Size: 355 887
SwaI ATTT'AAAT
Cuts at: 0 1194 1242
Size: 1194 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 589 1242
Size: 589 653
TatI w'GTAC_w
Cuts at: 0 330 1242
Size: 330 912
XmaI C'CCGG_G
Cuts at: 0 186 1242
Size: 186 1056
XmnI GAAnn'nnTTC
Cuts at: 0 314 518 1242
Size: 314 204 724
Fragments arranged by size:
724 314 204
Enzymes that do cut:
Acc65I AcuI AleI ApoI AseI AvaI BanI BanII
BbeI BciVI BglI Bme1580I BsaHI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI Bsu36I
BtgI BtsI DraI EaeI EagI EarI EcoICRI Eco57MI
EcoNI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV
PfoI PsrI SacI SacII SapI SfcI SfiI SfoI
SmaI SrfI SspI StyI SwaI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI
BsmI BsmBI BspEI BspMI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EciI EcoO109I EcoRI EcoRV FalI FseI HindIII
MfeI MluI MscI NcoI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SmlI SnaBI SpeI SphI StuI TstI Tth111I XbaI
XcmI XhoI ZraI