Restriction map for
pFN21AE0714
(Linear) (Six-base) MAPSORT of: KIEE0714.seq Check: 1237 from: 1 to: 1233
KIEE0714.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 1233
Size: 18 1215
AflII C'TTAA_G
Cuts at: 0 861 1233
Size: 861 372
AlwNI CAG_nnn'CTG
Cuts at: 0 151 286 1233
Size: 151 135 947
Fragments arranged by size:
947 151 135
ApaI G_GGCC'C
Cuts at: 0 175 1233
Size: 175 1058
AvaI C'yCGr_G
Cuts at: 0 62 177 1233
Size: 62 115 1056
Fragments arranged by size:
1056 115 62
BamHI G'GATC_C
Cuts at: 0 1093 1233
Size: 1093 140
BanII G_rGCy'C
Cuts at: 0 123 175 252 1012 1233
Size: 123 52 77 760 221
Fragments arranged by size:
760 221 123 77 52
BbvCI CC'TCA_GC
Cuts at: 0 164 1233
Size: 164 1069
BlpI GC'TnA_GC
Cuts at: 0 4 1233
Size: 4 1229
Bme1580I G_kGCm'C
Cuts at: 0 175 1233
Size: 175 1058
BmrI ACTGGGnnnn_n'
Cuts at: 0 418 1035 1233
Size: 418 617 198
Fragments arranged by size:
617 418 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1113 1203 1233
Size: 1113 90 30
Fragments arranged by size:
1113 90 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1233
Size: 61 1172
Bpu10I CC'TnA_GC
Cuts at: 0 164 927 939 1233
Size: 164 763 12 294
Fragments arranged by size:
763 294 164 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 1169 1233
Size: 1169 64
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 131 1192 1233
Size: 131 1061 41
Fragments arranged by size:
1061 131 41
BseYI C'CCAG_C
Cuts at: 0 89 264 315 369 393 477 808 813
Size: 89 175 51 54 24 84 331 5
Cuts at: 813 951 981 987 1233
Size: 138 30 6 246
Fragments arranged by size:
331 246 175 138 89 84 54 51
30 24 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 1233
Size: 264 969
Bsp1286I G_dGCh'C
Cuts at: 0 123 175 252 1012 1233
Size: 123 52 77 760 221
Fragments arranged by size:
760 221 123 77 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 1233
Size: 18 1215
BsrBI CCG'CTC
Cuts at: 0 69 1233
Size: 69 1164
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 151 1233
Size: 24 127 1082
Fragments arranged by size:
1082 127 24
BstYI r'GATC_y
Cuts at: 0 323 377 431 821 1093 1233
Size: 323 54 54 390 272 140
Fragments arranged by size:
390 323 272 140 54 54
Bsu36I CC'TnA_GG
Cuts at: 0 10 1233
Size: 10 1223
BtgI C'CryG_G
Cuts at: 0 447 1233
Size: 447 786
EaeI y'GGCC_r
Cuts at: 0 870 1233
Size: 870 363
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 1233
Size: 211 1022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1233
Size: 61 1172
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 1233
Size: 220 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 171 172 283 853 1210 1233
Size: 171 1 111 570 357 23
Fragments arranged by size:
570 357 171 111 23 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1085 1117 1233
Size: 1085 32 116
Fragments arranged by size:
1085 116 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1233
Size: 1046 187
MspA1I CmG'CkG
Cuts at: 0 24 168 193 268 1233
Size: 24 144 25 75 965
Fragments arranged by size:
965 144 75 25 24
PasI CC'CwG_GG
Cuts at: 0 100 238 1233
Size: 100 138 995
Fragments arranged by size:
995 138 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 286 340 448 1233
Size: 286 54 108 785
Fragments arranged by size:
785 286 108 54
PpuMI rG'GwC_Cy
Cuts at: 0 853 1233
Size: 853 380
PspOMI G'GGCC_C
Cuts at: 0 171 1233
Size: 171 1062
PstI C_TGCA'G
Cuts at: 0 29 147 214 1233
Size: 29 118 67 1019
Fragments arranged by size:
1019 118 67 29
PvuII CAG'CTG
Cuts at: 0 24 268 1233
Size: 24 244 965
Fragments arranged by size:
965 244 24
SfcI C'TryA_G
Cuts at: 0 25 143 210 1233
Size: 25 118 67 1023
Fragments arranged by size:
1023 118 67 25
SmlI C'TyrA_G
Cuts at: 0 861 1233
Size: 861 372
StuI AGG'CCT
Cuts at: 0 226 746 902 1233
Size: 226 520 156 331
Fragments arranged by size:
520 331 226 156
StyI C'CwwG_G
Cuts at: 0 843 1085 1233
Size: 843 242 148
Fragments arranged by size:
843 242 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 230 1054 1233
Size: 230 824 179
Fragments arranged by size:
824 230 179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 97 229 995 1180 1221 1233
Size: 97 132 766 185 41 12
Fragments arranged by size:
766 185 132 97 41 12
Enzymes that do cut:
AarI AflII AlwNI ApaI AvaI BamHI BanII BbvCI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BstAPI BstYI Bsu36I
BtgI EaeI EciI Eco57MI EcoNI EcoO109I Hin4I MmeI
MspA1I PasI PflMI PpuMI PspOMI PstI PvuII SfcI
SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI