Restriction map for
pFN21AE0658
(Linear) (Six-base) MAPSORT of: KIEE0658.seq Check: 7791 from: 1 to: 1167
KIEE0658.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 501 1167
Size: 345 156 666
Fragments arranged by size:
666 345 156
AccI GT'mk_AC
Cuts at: 0 623 1167
Size: 623 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1167
Size: 1075 92
AfeI AGC'GCT
Cuts at: 0 1027 1167
Size: 1027 140
AlwNI CAG_nnn'CTG
Cuts at: 0 279 291 432 968 1167
Size: 279 12 141 536 199
Fragments arranged by size:
536 279 199 141 12
ApaI G_GGCC'C
Cuts at: 0 323 1167
Size: 323 844
ApaLI G'TGCA_C
Cuts at: 0 514 1167
Size: 514 653
BanI G'GyrC_C
Cuts at: 0 726 1167
Size: 726 441
BanII G_rGCy'C
Cuts at: 0 323 1167
Size: 323 844
BbeI G_GCGC'C
Cuts at: 0 730 1167
Size: 730 437
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 561 1167
Size: 543 18 606
Fragments arranged by size:
606 543 18
BglII A'GATC_T
Cuts at: 0 677 1167
Size: 677 490
BlpI GC'TnA_GC
Cuts at: 0 160 1167
Size: 160 1007
Bme1580I G_kGCm'C
Cuts at: 0 323 518 1167
Size: 323 195 649
Fragments arranged by size:
649 323 195
BmtI G_CTAG'C
Cuts at: 0 619 1167
Size: 619 548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1116 1167
Size: 45 1071 51
Fragments arranged by size:
1071 51 45
Bpu10I CC'TnA_GC
Cuts at: 0 241 1117 1167
Size: 241 876 50
Fragments arranged by size:
876 241 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1167
Size: 682 485
BsaAI yAC'GTr
Cuts at: 0 479 1167
Size: 479 688
BsaHI Gr'CG_yC
Cuts at: 0 548 727 865 1065 1167
Size: 548 179 138 200 102
Fragments arranged by size:
548 200 179 138 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1090 1120 1167
Size: 1090 30 47
Fragments arranged by size:
1090 47 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1155 1167
Size: 1155 12
BseYI C'CCAG_C
Cuts at: 0 560 1151 1167
Size: 560 591 16
Fragments arranged by size:
591 560 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1042 1167
Size: 901 141 125
Fragments arranged by size:
901 141 125
BsiEI CG_ry'CG
Cuts at: 0 459 722 1167
Size: 459 263 445
Fragments arranged by size:
459 445 263
BsiHKAI G_wGCw'C
Cuts at: 0 473 518 647 1167
Size: 473 45 129 520
Fragments arranged by size:
520 473 129 45
BsmI GAATG_Cn'
Cuts at: 0 174 1167
Size: 174 993
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1069 1167
Size: 1069 98
Bsp1286I G_dGCh'C
Cuts at: 0 323 473 518 647 1167
Size: 323 150 45 129 520
Fragments arranged by size:
520 323 150 129 45
BspHI T'CATG_A
Cuts at: 0 230 1167
Size: 230 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 345 392 501 1167
Size: 335 10 47 109 666
Fragments arranged by size:
666 335 109 47 10
BsrBI CCG'CTC
Cuts at: 0 164 1167
Size: 164 1003
BsrFI r'CCGG_y
Cuts at: 0 633 1167
Size: 633 534
BssHII G'CGCG_C
Cuts at: 0 8 115 503 1167
Size: 8 107 388 664
Fragments arranged by size:
664 388 107 8
BssSI C'ACGA_G
Cuts at: 0 1018 1167
Size: 1018 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 690 1167
Size: 690 477
BstYI r'GATC_y
Cuts at: 0 677 809 980 1167
Size: 677 132 171 187
Fragments arranged by size:
677 187 171 132
BtgI C'CryG_G
Cuts at: 0 94 1160 1167
Size: 94 1066 7
Fragments arranged by size:
1066 94 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 231 764 1108 1167
Size: 231 533 344 59
Fragments arranged by size:
533 344 231 59
BtsI GCAGTG_nn'
Cuts at: 0 272 473 1167
Size: 272 201 694
Fragments arranged by size:
694 272 201
EaeI y'GGCC_r
Cuts at: 0 416 497 719 950 1167
Size: 416 81 222 231 217
Fragments arranged by size:
416 231 222 217 81
EagI C'GGCC_G
Cuts at: 0 719 1167
Size: 719 448
EarI CTCTTCn'nnn_
Cuts at: 0 226 1012 1167
Size: 226 786 155
Fragments arranged by size:
786 226 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1032 1167
Size: 1032 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1075 1116 1167
Size: 45 1030 41 51
Fragments arranged by size:
1030 51 45 41
EcoO109I rG'GnC_Cy
Cuts at: 0 108 586 1167
Size: 108 478 581
Fragments arranged by size:
581 478 108
FspI TGC'GCA
Cuts at: 0 193 1167
Size: 193 974
HaeII r_GCGC'y
Cuts at: 0 32 53 160 190 730 1029 1167
Size: 32 21 107 30 540 299 138
Fragments arranged by size:
540 299 138 107 32 30 21
KasI G'GCGC_C
Cuts at: 0 726 1167
Size: 726 441
MscI TGG'CCA
Cuts at: 0 418 499 952 1167
Size: 418 81 453 215
Fragments arranged by size:
453 418 215 81
MslI CAynn'nnrTG
Cuts at: 0 838 940 1167
Size: 838 102 227
Fragments arranged by size:
838 227 102
MspA1I CmG'CkG
Cuts at: 0 96 410 432 706 712 965 1167
Size: 96 314 22 274 6 253 202
Fragments arranged by size:
314 274 253 202 96 22 6
NarI GG'CG_CC
Cuts at: 0 727 1167
Size: 727 440
NheI G'CTAG_C
Cuts at: 0 615 1167
Size: 615 552
NspI r_CATG'y
Cuts at: 0 369 990 1167
Size: 369 621 177
Fragments arranged by size:
621 369 177
PasI CC'CwG_GG
Cuts at: 0 581 1167
Size: 581 586
PmlI CAC'GTG
Cuts at: 0 479 1167
Size: 479 688
PpuMI rG'GwC_Cy
Cuts at: 0 586 1167
Size: 586 581
PspOMI G'GGCC_C
Cuts at: 0 319 1167
Size: 319 848
PstI C_TGCA'G
Cuts at: 0 711 1167
Size: 711 456
PvuII CAG'CTG
Cuts at: 0 965 1167
Size: 965 202
SacII CC_GC'GG
Cuts at: 0 97 1167
Size: 97 1070
SanDI GG'GwC_CC
Cuts at: 0 586 1167
Size: 586 581
ScaI AGT'ACT
Cuts at: 0 349 1167
Size: 349 818
SexAI A'CCwGG_T
Cuts at: 0 254 1167
Size: 254 913
SfcI C'TryA_G
Cuts at: 0 707 1167
Size: 707 460
SfoI GGC'GCC
Cuts at: 0 728 1167
Size: 728 439
SmlI C'TyrA_G
Cuts at: 0 697 1167
Size: 697 470
StuI AGG'CCT
Cuts at: 0 595 1167
Size: 595 572
StyI C'CwwG_G
Cuts at: 0 590 800 914 1167
Size: 590 210 114 253
Fragments arranged by size:
590 253 210 114
TatI w'GTAC_w
Cuts at: 0 347 1167
Size: 347 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 159 191 1167
Size: 159 32 976
Fragments arranged by size:
976 159 32
Tth111I GACn'n_nGTC
Cuts at: 0 547 1167
Size: 547 620
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI ApaLI BanI
BanII BbeI BbsI BglII BlpI Bme1580I BmtI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstYI BtgI BtgZI BtsI
EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII
KasI MscI MslI MspA1I NarI NheI NspI PasI
PmlI PpuMI PspOMI PstI PvuII SacII SanDI ScaI
SexAI SfcI SfoI SmlI StuI StyI TatI TstI
Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BmrI BplI BsaI BsaBI BsaWI BsiWI BspEI
BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XcmI XhoI
XmaI XmnI ZraI