Restriction map for
pFN21AE0643
(Linear) (Six-base) MAPSORT of: KIEE0643.seq Check: 9273 from: 1 to: 1302
KIEE0643.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 692 1302
Size: 692 610
AccI GT'mk_AC
Cuts at: 0 692 1052 1302
Size: 692 360 250
Fragments arranged by size:
692 360 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1302
Size: 901 401
AfeI AGC'GCT
Cuts at: 0 1035 1302
Size: 1035 267
AflIII A'CryG_T
Cuts at: 0 284 695 1302
Size: 284 411 607
Fragments arranged by size:
607 411 284
AleI CACnn'nnGTG
Cuts at: 0 184 1302
Size: 184 1118
AlwNI CAG_nnn'CTG
Cuts at: 0 381 879 1302
Size: 381 498 423
Fragments arranged by size:
498 423 381
ApaI G_GGCC'C
Cuts at: 0 218 229 971 1160 1302
Size: 218 11 742 189 142
Fragments arranged by size:
742 218 189 142 11
AscI GG'CGCG_CC
Cuts at: 0 608 1302
Size: 608 694
AvaI C'yCGr_G
Cuts at: 0 433 1160 1302
Size: 433 727 142
Fragments arranged by size:
727 433 142
BanI G'GyrC_C
Cuts at: 0 36 400 680 782 1302
Size: 36 364 280 102 520
Fragments arranged by size:
520 364 280 102 36
BanII G_rGCy'C
Cuts at: 0 102 218 229 338 829 840 971 1114
Size: 102 116 11 109 491 11 131 143
Cuts at: 1114 1160 1302
Size: 46 142
Fragments arranged by size:
491 143 142 131 116 109 102 46
11 11
BbeI G_GCGC'C
Cuts at: 0 40 404 1302
Size: 40 364 898
Fragments arranged by size:
898 364 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 1302
Size: 448 854
BglI GCCn_nnn'nGGC
Cuts at: 0 203 678 802 946 1302
Size: 203 475 124 144 356
Fragments arranged by size:
475 356 203 144 124
Bme1580I G_kGCm'C
Cuts at: 0 56 218 229 298 489 971 1160 1302
Size: 56 162 11 69 191 482 189 142
Fragments arranged by size:
482 191 189 162 142 69 56 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 990 1302
Size: 990 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1302
Size: 79 1223
BsaHI Gr'CG_yC
Cuts at: 0 37 401 746 1302
Size: 37 364 345 556
Fragments arranged by size:
556 364 345 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 1302
Size: 751 30 521
Fragments arranged by size:
751 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1165 1302
Size: 1165 137
BseYI C'CCAG_C
Cuts at: 0 63 68 118 327 847 1107 1302
Size: 63 5 50 209 520 260 195
Fragments arranged by size:
520 260 209 195 63 50 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 892 1302
Size: 280 612 410
Fragments arranged by size:
612 410 280
BsiEI CG_ry'CG
Cuts at: 0 468 472 620 790 953 1302
Size: 468 4 148 170 163 349
Fragments arranged by size:
468 349 170 163 148 4
BsiHKAI G_wGCw'C
Cuts at: 0 425 1302
Size: 425 877
Bsp1286I G_dGCh'C
Cuts at: 0 56 102 218 229 298 338 425 489
Size: 56 46 116 11 69 40 87 64
Cuts at: 489 829 840 971 1114 1160 1302
Size: 340 11 131 143 46 142
Fragments arranged by size:
340 143 142 131 116 87 69 64
56 46 46 40 11 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 692 1302
Size: 678 14 610
Fragments arranged by size:
678 610 14
BsrFI r'CCGG_y
Cuts at: 0 33 440 467 785 1079 1302
Size: 33 407 27 318 294 223
Fragments arranged by size:
407 318 294 223 33 27
BssHII G'CGCG_C
Cuts at: 0 444 608 851 1124 1302
Size: 444 164 243 273 178
Fragments arranged by size:
444 273 243 178 164
BssSI C'ACGA_G
Cuts at: 0 415 1302
Size: 415 887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 1302
Size: 381 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 1302
Size: 63 1239
BstYI r'GATC_y
Cuts at: 0 6 656 1302
Size: 6 650 646
Fragments arranged by size:
650 646 6
BtgI C'CryG_G
Cuts at: 0 347 651 800 971 1302
Size: 347 304 149 171 331
Fragments arranged by size:
347 331 304 171 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 1036 1302
Size: 411 625 266
Fragments arranged by size:
625 411 266
BtsI GCAGTG_nn'
Cuts at: 0 514 1302
Size: 514 788
DraIII CAC_nnn'GTG
Cuts at: 0 417 1302
Size: 417 885
EaeI y'GGCC_r
Cuts at: 0 344 465 469 617 631 756 787 950
Size: 344 121 4 148 14 125 31 163
Cuts at: 950 1025 1302
Size: 75 277
Fragments arranged by size:
344 277 163 148 125 121 75 31
14 4
EagI C'GGCC_G
Cuts at: 0 465 469 617 787 950 1302
Size: 465 4 148 170 163 352
Fragments arranged by size:
465 352 170 163 148 4
EarI CTCTTCn'nnn_
Cuts at: 0 1063 1302
Size: 1063 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 289 1302
Size: 121 168 1013
Fragments arranged by size:
1013 168 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1302
Size: 901 401
EcoO109I rG'GnC_Cy
Cuts at: 0 215 226 354 795 993 1156 1157 1302
Size: 215 11 128 441 198 163 1 145
Fragments arranged by size:
441 215 198 163 145 128 11 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 1302
Size: 264 1038
FseI GG_CCGG'CC
Cuts at: 0 471 1302
Size: 471 831
HaeII r_GCGC'y
Cuts at: 0 40 304 404 441 529 814 904 1009
Size: 40 264 100 37 88 285 90 105
Cuts at: 1009 1037 1177 1302
Size: 28 140 125
Fragments arranged by size:
285 264 140 125 105 100 90 88
40 37 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 816 848 982 1014 1302
Size: 547 32 237 32 134 32 288
Fragments arranged by size:
547 288 237 134 32 32 32
KasI G'GCGC_C
Cuts at: 0 36 400 1302
Size: 36 364 902
Fragments arranged by size:
902 364 36
MluI A'CGCG_T
Cuts at: 0 284 695 1302
Size: 284 411 607
Fragments arranged by size:
607 411 284
MscI TGG'CCA
Cuts at: 0 1027 1302
Size: 1027 275
MslI CAynn'nnrTG
Cuts at: 0 49 157 184 1302
Size: 49 108 27 1118
Fragments arranged by size:
1118 108 49 27
MspA1I CmG'CkG
Cuts at: 0 264 312 378 474 570 802 973 1095
Size: 264 48 66 96 96 232 171 122
Cuts at: 1095 1230 1302
Size: 135 72
Fragments arranged by size:
264 232 171 135 122 96 96 72
66 48
NaeI GCC'GGC
Cuts at: 0 35 442 469 1081 1302
Size: 35 407 27 612 221
Fragments arranged by size:
612 407 221 35 27
NarI GG'CG_CC
Cuts at: 0 37 401 1302
Size: 37 364 901
Fragments arranged by size:
901 364 37
NgoMIV G'CCGG_C
Cuts at: 0 33 440 467 1079 1302
Size: 33 407 27 612 223
Fragments arranged by size:
612 407 223 33 27
NotI GC'GGCC_GC
Cuts at: 0 950 1302
Size: 950 352
PasI CC'CwG_GG
Cuts at: 0 218 498 1302
Size: 218 280 804
Fragments arranged by size:
804 280 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 417 1302
Size: 417 885
PpuMI rG'GwC_Cy
Cuts at: 0 354 1302
Size: 354 948
PspOMI G'GGCC_C
Cuts at: 0 214 225 967 1156 1302
Size: 214 11 742 189 146
Fragments arranged by size:
742 214 189 146 11
PspXI vC'TCGA_Gb
Cuts at: 0 433 1302
Size: 433 869
PstI C_TGCA'G
Cuts at: 0 377 386 602 689 810 1302
Size: 377 9 216 87 121 492
Fragments arranged by size:
492 377 216 121 87 9
PvuII CAG'CTG
Cuts at: 0 264 312 378 1095 1302
Size: 264 48 66 717 207
Fragments arranged by size:
717 264 207 66 48
SacII CC_GC'GG
Cuts at: 0 803 974 1302
Size: 803 171 328
Fragments arranged by size:
803 328 171
SbfI CC_TGCA'GG
Cuts at: 0 689 1302
Size: 689 613
SfcI C'TryA_G
Cuts at: 0 373 382 598 685 806 1302
Size: 373 9 216 87 121 496
Fragments arranged by size:
496 373 216 121 87 9
SfoI GGC'GCC
Cuts at: 0 38 402 1302
Size: 38 364 900
Fragments arranged by size:
900 364 38
SgrAI Cr'CCGG_yG
Cuts at: 0 33 440 1302
Size: 33 407 862
Fragments arranged by size:
862 407 33
SmaI CCC'GGG
Cuts at: 0 1162 1302
Size: 1162 140
SmlI C'TyrA_G
Cuts at: 0 433 1302
Size: 433 869
StyI C'CwwG_G
Cuts at: 0 86 866 1302
Size: 86 780 436
Fragments arranged by size:
780 436 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 751 783 1302
Size: 346 32 373 32 519
Fragments arranged by size:
519 373 346 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1302
Size: 354 948
XhoI C'TCGA_G
Cuts at: 0 433 1302
Size: 433 869
XmaI C'CCGG_G
Cuts at: 0 1160 1302
Size: 1160 142
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
AscI AvaI BanI BanII BbeI BbsI BglI Bme1580I
BmrI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I KasI
MluI MscI MslI MspA1I NaeI NarI NgoMIV NotI
PasI PflMI PpuMI PspOMI PspXI PstI PvuII SacII
SbfI SfcI SfoI SgrAI SmaI SmlI StyI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoICRI
EcoNI EcoRI EcoRV FspI FspAI HincII HindIII HpaI
KpnI MfeI MmeI NcoI NdeI NheI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI SapI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XmnI
ZraI