Restriction map for
pFN21AE0612
(Linear) (Six-base) MAPSORT of: KIEE0612.seq Check: 1501 from: 1 to: 1089
KIEE0612.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AfeI AGC'GCT
Cuts at: 0 871 1089
Size: 871 218
AgeI A'CCGG_T
Cuts at: 0 744 1089
Size: 744 345
AlwNI CAG_nnn'CTG
Cuts at: 0 180 769 1089
Size: 180 589 320
Fragments arranged by size:
589 320 180
ApaI G_GGCC'C
Cuts at: 0 61 353 676 1083 1089
Size: 61 292 323 407 6
Fragments arranged by size:
407 323 292 61 6
ApoI r'AATT_y
Cuts at: 0 835 1089
Size: 835 254
AvaI C'yCGr_G
Cuts at: 0 60 409 467 1089
Size: 60 349 58 622
Fragments arranged by size:
622 349 60 58
BanI G'GyrC_C
Cuts at: 0 741 788 1089
Size: 741 47 301
Fragments arranged by size:
741 301 47
BanII G_rGCy'C
Cuts at: 0 61 353 362 488 625 676 684 992
Size: 61 292 9 126 137 51 8 308
Cuts at: 992 1083 1089
Size: 91 6
Fragments arranged by size:
308 292 137 126 91 61 51 9
8 6
BbeI G_GCGC'C
Cuts at: 0 792 1089
Size: 792 297
BbvCI CC'TCA_GC
Cuts at: 0 731 1089
Size: 731 358
BglI GCCn_nnn'nGGC
Cuts at: 0 156 1089
Size: 156 933
BglII A'GATC_T
Cuts at: 0 917 1089
Size: 917 172
Bme1580I G_kGCm'C
Cuts at: 0 61 67 287 353 676 744 1083 1089
Size: 61 6 220 66 323 68 339 6
Fragments arranged by size:
339 323 220 68 66 61 6 6
BmtI G_CTAG'C
Cuts at: 0 229 1089
Size: 229 860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 746 1089
Size: 595 151 343
Fragments arranged by size:
595 343 151
Bpu10I CC'TnA_GC
Cuts at: 0 624 731 856 1089
Size: 624 107 125 233
Fragments arranged by size:
624 233 125 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1089
Size: 877 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 1089
Size: 584 505
BsaHI Gr'CG_yC
Cuts at: 0 426 789 1089
Size: 426 363 300
Fragments arranged by size:
426 363 300
BsaWI w'CCGG_w
Cuts at: 0 744 1089
Size: 744 345
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 748 1089
Size: 580 168 341
Fragments arranged by size:
580 341 168
BseYI C'CCAG_C
Cuts at: 0 270 316 594 1089
Size: 270 46 278 495
Fragments arranged by size:
495 278 270 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1089
Size: 928 161
BsiEI CG_ry'CG
Cuts at: 0 160 243 423 1089
Size: 160 83 180 666
Fragments arranged by size:
666 180 160 83
BsiHKAI G_wGCw'C
Cuts at: 0 362 1089
Size: 362 727
Bsp1286I G_dGCh'C
Cuts at: 0 61 67 287 353 362 488 625 676
Size: 61 6 220 66 9 126 137 51
Cuts at: 676 684 744 992 1083 1089
Size: 8 60 248 91 6
Fragments arranged by size:
248 220 137 126 91 66 61 60
51 9 8 6 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 900 1089
Size: 900 189
BsrBI CCG'CTC
Cuts at: 0 162 1089
Size: 162 927
BsrDI GCAATG_nn'
Cuts at: 0 840 972 1089
Size: 840 132 117
Fragments arranged by size:
840 132 117
BsrFI r'CCGG_y
Cuts at: 0 130 155 238 462 744 1089
Size: 130 25 83 224 282 345
Fragments arranged by size:
345 282 224 130 83 25
BsrGI T'GTAC_A
Cuts at: 0 125 551 1089
Size: 125 426 538
Fragments arranged by size:
538 426 125
BssSI C'ACGA_G
Cuts at: 0 163 1089
Size: 163 926
BstYI r'GATC_y
Cuts at: 0 917 1089
Size: 917 172
BtsI GCAGTG_nn'
Cuts at: 0 65 389 1089
Size: 65 324 700
Fragments arranged by size:
700 324 65
EaeI y'GGCC_r
Cuts at: 0 114 157 240 420 1089
Size: 114 43 83 180 669
Fragments arranged by size:
669 180 114 83 43
EagI C'GGCC_G
Cuts at: 0 157 240 420 1089
Size: 157 83 180 669
Fragments arranged by size:
669 180 157 83
EarI CTCTTCn'nnn_
Cuts at: 0 611 689 1089
Size: 611 78 400
Fragments arranged by size:
611 400 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 488 1089
Size: 488 601
EcoICRI GAG'CTC
Cuts at: 0 360 1089
Size: 360 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 746 1089
Size: 595 151 343
Fragments arranged by size:
595 343 151
EcoO109I rG'GnC_Cy
Cuts at: 0 57 350 672 691 1079 1089
Size: 57 293 322 19 388 10
Fragments arranged by size:
388 322 293 57 19 10
HaeII r_GCGC'y
Cuts at: 0 26 181 301 792 873 1089
Size: 26 155 120 491 81 216
Fragments arranged by size:
491 216 155 120 81 26
HincII GTy'rAC
Cuts at: 0 1035 1089
Size: 1035 54
KasI G'GCGC_C
Cuts at: 0 788 1089
Size: 788 301
MslI CAynn'nnrTG
Cuts at: 0 145 1024 1089
Size: 145 879 65
Fragments arranged by size:
879 145 65
MspA1I CmG'CkG
Cuts at: 0 6 195 202 373 1089
Size: 6 189 7 171 716
Fragments arranged by size:
716 189 171 7 6
NaeI GCC'GGC
Cuts at: 0 240 464 1089
Size: 240 224 625
Fragments arranged by size:
625 240 224
NarI GG'CG_CC
Cuts at: 0 789 1089
Size: 789 300
NgoMIV G'CCGG_C
Cuts at: 0 238 462 1089
Size: 238 224 627
Fragments arranged by size:
627 238 224
NheI G'CTAG_C
Cuts at: 0 225 1089
Size: 225 864
NspI r_CATG'y
Cuts at: 0 1033 1089
Size: 1033 56
PasI CC'CwG_GG
Cuts at: 0 28 676 1089
Size: 28 648 413
Fragments arranged by size:
648 413 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 436 1068 1089
Size: 436 632 21
Fragments arranged by size:
632 436 21
PfoI T'CCnGG_A
Cuts at: 0 653 1089
Size: 653 436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 963 995 1089
Size: 963 32 94
Fragments arranged by size:
963 94 32
PpuMI rG'GwC_Cy
Cuts at: 0 691 1089
Size: 691 398
PspOMI G'GGCC_C
Cuts at: 0 57 349 672 1079 1089
Size: 57 292 323 407 10
Fragments arranged by size:
407 323 292 57 10
PstI C_TGCA'G
Cuts at: 0 669 1089
Size: 669 420
SacI G_AGCT'C
Cuts at: 0 362 1089
Size: 362 727
SapI GCTCTTCn'nnn_
Cuts at: 0 689 1089
Size: 689 400
SfcI C'TryA_G
Cuts at: 0 665 1089
Size: 665 424
SfoI GGC'GCC
Cuts at: 0 790 1089
Size: 790 299
SgrAI Cr'CCGG_yG
Cuts at: 0 744 1089
Size: 744 345
SmaI CCC'GGG
Cuts at: 0 62 469 1089
Size: 62 407 620
Fragments arranged by size:
620 407 62
SmlI C'TyrA_G
Cuts at: 0 892 1089
Size: 892 197
SrfI GCCC'GGGC
Cuts at: 0 62 1089
Size: 62 1027
TatI w'GTAC_w
Cuts at: 0 125 551 1089
Size: 125 426 538
Fragments arranged by size:
538 426 125
XmaI C'CCGG_G
Cuts at: 0 60 467 1089
Size: 60 407 622
Fragments arranged by size:
622 407 60
Enzymes that do cut:
AfeI AgeI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbvCI BglI BglII Bme1580I BmtI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstYI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII HincII KasI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI SacI SapI SfcI SfoI SgrAI SmaI
SmlI SrfI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BplI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
MscI NcoI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI