Restriction map for
pFN21AE0602
(Linear) (Six-base) MAPSORT of: KIEE0602.seq Check: 6197 from: 1 to: 1074
KIEE0602.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
Acc65I G'GTAC_C
Cuts at: 0 294 500 1074
Size: 294 206 574
Fragments arranged by size:
574 294 206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 690 752 1074
Size: 195 495 62 322
Fragments arranged by size:
495 322 195 62
AflIII A'CryG_T
Cuts at: 0 832 1074
Size: 832 242
AlwNI CAG_nnn'CTG
Cuts at: 0 333 730 1074
Size: 333 397 344
Fragments arranged by size:
397 344 333
ApoI r'AATT_y
Cuts at: 0 345 719 757 1074
Size: 345 374 38 317
Fragments arranged by size:
374 345 317 38
AvrII C'CTAG_G
Cuts at: 0 670 1074
Size: 670 404
BamHI G'GATC_C
Cuts at: 0 40 1074
Size: 40 1034
BanI G'GyrC_C
Cuts at: 0 294 500 674 1074
Size: 294 206 174 400
Fragments arranged by size:
400 294 206 174
BanII G_rGCy'C
Cuts at: 0 791 1074
Size: 791 283
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 655 925 1074
Size: 201 454 270 149
Fragments arranged by size:
454 270 201 149
BglI GCCn_nnn'nGGC
Cuts at: 0 606 1074
Size: 606 468
BlpI GC'TnA_GC
Cuts at: 0 581 1074
Size: 581 493
BmgBI CAC'GTC
Cuts at: 0 381 1074
Size: 381 693
Bpu10I CC'TnA_GC
Cuts at: 0 512 1074
Size: 512 562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 160 1074
Size: 151 9 914
Fragments arranged by size:
914 151 9
BsaAI yAC'GTr
Cuts at: 0 486 1074
Size: 486 588
BsaBI GATnn'nnATC
Cuts at: 0 626 1074
Size: 626 448
BsmI GAATG_Cn'
Cuts at: 0 167 1074
Size: 167 907
BsmBI CGTCTCn'nnnn_
Cuts at: 0 371 1074
Size: 371 703
Bsp1286I G_dGCh'C
Cuts at: 0 791 1074
Size: 791 283
BsrBI CCG'CTC
Cuts at: 0 506 1074
Size: 506 568
BssSI C'ACGA_G
Cuts at: 0 307 1074
Size: 307 767
BstAPI GCAn_nnn'nTGC
Cuts at: 0 333 1074
Size: 333 741
BstXI CCAn_nnnn'nTGG
Cuts at: 0 991 1074
Size: 991 83
BstYI r'GATC_y
Cuts at: 0 40 660 1074
Size: 40 620 414
Fragments arranged by size:
620 414 40
BtsI GCAGTG_nn'
Cuts at: 0 331 945 1074
Size: 331 614 129
Fragments arranged by size:
614 331 129
DraIII CAC_nnn'GTG
Cuts at: 0 237 1074
Size: 237 837
EaeI y'GGCC_r
Cuts at: 0 92 607 1074
Size: 92 515 467
Fragments arranged by size:
515 467 92
EarI CTCTTCn'nnn_
Cuts at: 0 307 1074
Size: 307 767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 690 752 1074
Size: 195 495 62 322
Fragments arranged by size:
495 322 195 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 982 1074
Size: 982 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 1074
Size: 574 32 468
Fragments arranged by size:
574 468 32
HincII GTy'rAC
Cuts at: 0 195 1074
Size: 195 879
HindIII A'AGCT_T
Cuts at: 0 424 1074
Size: 424 650
HpaI GTT'AAC
Cuts at: 0 195 1074
Size: 195 879
KpnI G_GTAC'C
Cuts at: 0 298 504 1074
Size: 298 206 570
Fragments arranged by size:
570 298 206
MfeI C'AATT_G
Cuts at: 0 363 563 1074
Size: 363 200 511
Fragments arranged by size:
511 363 200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1074
Size: 254 820
MscI TGG'CCA
Cuts at: 0 94 609 1074
Size: 94 515 465
Fragments arranged by size:
515 465 94
MspA1I CmG'CkG
Cuts at: 0 633 843 1074
Size: 633 210 231
Fragments arranged by size:
633 231 210
NspI r_CATG'y
Cuts at: 0 836 1074
Size: 836 238
PasI CC'CwG_GG
Cuts at: 0 783 1074
Size: 783 291
PciI A'CATG_T
Cuts at: 0 832 1074
Size: 832 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 1074
Size: 273 801
PfoI T'CCnGG_A
Cuts at: 0 200 1074
Size: 200 874
PshAI GACnn'nnGTC
Cuts at: 0 80 1074
Size: 80 994
PvuII CAG'CTG
Cuts at: 0 633 843 1074
Size: 633 210 231
Fragments arranged by size:
633 231 210
SapI GCTCTTCn'nnn_
Cuts at: 0 307 1074
Size: 307 767
ScaI AGT'ACT
Cuts at: 0 703 1030 1074
Size: 703 327 44
Fragments arranged by size:
703 327 44
SexAI A'CCwGG_T
Cuts at: 0 233 493 1074
Size: 233 260 581
Fragments arranged by size:
581 260 233
SmlI C'TyrA_G
Cuts at: 0 166 175 1074
Size: 166 9 899
Fragments arranged by size:
899 166 9
SspI AAT'ATT
Cuts at: 0 247 1074
Size: 247 827
StyI C'CwwG_G
Cuts at: 0 653 670 1074
Size: 653 17 404
Fragments arranged by size:
653 404 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 869 1074
Size: 477 392 205
Fragments arranged by size:
477 392 205
TatI w'GTAC_w
Cuts at: 0 701 1028 1074
Size: 701 327 46
Fragments arranged by size:
701 327 46
Tth111I GACn'n_nGTC
Cuts at: 0 127 1074
Size: 127 947
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI AvrII BamHI BanI
BanII BbsI BglI BlpI BmgBI Bpu10I BpuEI BsaAI
BsaBI BsmI BsmBI Bsp1286I BsrBI BssSI BstAPI BstXI
BstYI BtsI DraIII EaeI EarI Eco57MI EcoNI Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MscI MspA1I
NspI PasI PciI PflMI PfoI PshAI PvuII SapI
ScaI SexAI SmlI SspI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BmtI BplI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII KasI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI