Restriction map for
pFN21AE0591
(Linear) (Six-base) MAPSORT of: KIEE0591.seq Check: 6406 from: 1 to: 1236
KIEE0591.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 515 1236
Size: 515 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 478 724 797 1162 1183 1236
Size: 378 100 246 73 365 21 53
Fragments arranged by size:
378 365 246 100 73 53 21
AfeI AGC'GCT
Cuts at: 0 1189 1236
Size: 1189 47
AflIII A'CryG_T
Cuts at: 0 62 691 693 1236
Size: 62 629 2 543
Fragments arranged by size:
629 543 62 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 1236
Size: 649 587
AlwNI CAG_nnn'CTG
Cuts at: 0 1222 1236
Size: 1222 14
ApaI G_GGCC'C
Cuts at: 0 149 286 1236
Size: 149 137 950
Fragments arranged by size:
950 149 137
ApoI r'AATT_y
Cuts at: 0 661 1236
Size: 661 575
AvaI C'yCGr_G
Cuts at: 0 149 279 291 1236
Size: 149 130 12 945
Fragments arranged by size:
945 149 130 12
BanI G'GyrC_C
Cuts at: 0 196 223 1236
Size: 196 27 1013
Fragments arranged by size:
1013 196 27
BanII G_rGCy'C
Cuts at: 0 149 286 1214 1236
Size: 149 137 928 22
Fragments arranged by size:
928 149 137 22
BbeI G_GCGC'C
Cuts at: 0 227 1236
Size: 227 1009
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 785 1236
Size: 620 165 451
Fragments arranged by size:
620 451 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 389 1236
Size: 355 34 847
Fragments arranged by size:
847 355 34
BglI GCCn_nnn'nGGC
Cuts at: 0 526 877 1236
Size: 526 351 359
Fragments arranged by size:
526 359 351
BglII A'GATC_T
Cuts at: 0 95 1236
Size: 95 1141
BlpI GC'TnA_GC
Cuts at: 0 1228 1236
Size: 1228 8
Bme1580I G_kGCm'C
Cuts at: 0 70 149 286 385 701 1236
Size: 70 79 137 99 316 535
Fragments arranged by size:
535 316 137 99 79 70
BmgBI CAC'GTC
Cuts at: 0 63 1236
Size: 63 1173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 769 811 1072 1236
Size: 258 511 42 261 164
Fragments arranged by size:
511 261 258 164 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1236
Size: 805 431
BsaI GGTCTCn'nnnn_
Cuts at: 0 577 1236
Size: 577 659
BsaAI yAC'GTr
Cuts at: 0 694 915 1236
Size: 694 221 321
Fragments arranged by size:
694 321 221
BsaHI Gr'CG_yC
Cuts at: 0 140 170 224 344 890 1236
Size: 140 30 54 120 546 346
Fragments arranged by size:
546 346 140 120 54 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 808 1018 1236
Size: 808 210 218
Fragments arranged by size:
808 218 210
BseYI C'CCAG_C
Cuts at: 0 286 298 1236
Size: 286 12 938
Fragments arranged by size:
938 286 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 354 417 487 1236
Size: 143 211 63 70 749
Fragments arranged by size:
749 211 143 70 63
BsiEI CG_ry'CG
Cuts at: 0 204 313 1236
Size: 204 109 923
Fragments arranged by size:
923 204 109
BsiHKAI G_wGCw'C
Cuts at: 0 1214 1229 1236
Size: 1214 15 7
Fragments arranged by size:
1214 15 7
Bsp1286I G_dGCh'C
Cuts at: 0 70 149 286 385 701 1214 1229 1236
Size: 70 79 137 99 316 513 15 7
Fragments arranged by size:
513 316 137 99 79 70 15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 1236
Size: 515 721
BsrBI CCG'CTC
Cuts at: 0 798 1236
Size: 798 438
BsrFI r'CCGG_y
Cuts at: 0 193 199 762 1236
Size: 193 6 563 474
Fragments arranged by size:
563 474 193 6
BssHII G'CGCG_C
Cuts at: 0 178 1236
Size: 178 1058
BstAPI GCAn_nnn'nTGC
Cuts at: 0 510 846 1236
Size: 510 336 390
Fragments arranged by size:
510 390 336
BstEII G'GTnAC_C
Cuts at: 0 1056 1236
Size: 1056 180
BstYI r'GATC_y
Cuts at: 0 95 638 1236
Size: 95 543 598
Fragments arranged by size:
598 543 95
Bsu36I CC'TnA_GG
Cuts at: 0 928 1236
Size: 928 308
BtgI C'CryG_G
Cuts at: 0 121 884 1236
Size: 121 763 352
Fragments arranged by size:
763 352 121
BtsI GCAGTG_nn'
Cuts at: 0 371 429 1236
Size: 371 58 807
Fragments arranged by size:
807 371 58
DrdI GACnn_nn'nnGTC
Cuts at: 0 493 1236
Size: 493 743
EaeI y'GGCC_r
Cuts at: 0 53 191 201 310 878 1037 1236
Size: 53 138 10 109 568 159 199
Fragments arranged by size:
568 199 159 138 109 53 10
EagI C'GGCC_G
Cuts at: 0 201 310 1236
Size: 201 109 926
Fragments arranged by size:
926 201 109
EarI CTCTTCn'nnn_
Cuts at: 0 262 1236
Size: 262 974
EcoICRI GAG'CTC
Cuts at: 0 1212 1236
Size: 1212 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 378 478 724 769 797 811 1072
Size: 258 120 100 246 45 28 14 261
Cuts at: 1072 1162 1183 1236
Size: 90 21 53
Fragments arranged by size:
261 258 246 120 100 90 53 45
28 21 14
EcoO109I rG'GnC_Cy
Cuts at: 0 126 146 283 295 453 1032 1236
Size: 126 20 137 12 158 579 204
Fragments arranged by size:
579 204 158 137 126 20 12
HaeII r_GCGC'y
Cuts at: 0 227 1140 1191 1236
Size: 227 913 51 45
Fragments arranged by size:
913 227 51 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 934 966 1236
Size: 934 32 270
Fragments arranged by size:
934 270 32
KasI G'GCGC_C
Cuts at: 0 223 1236
Size: 223 1013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 242 341 1112 1236
Size: 125 117 99 771 124
Fragments arranged by size:
771 125 124 117 99
MscI TGG'CCA
Cuts at: 0 55 1039 1236
Size: 55 984 197
Fragments arranged by size:
984 197 55
MslI CAynn'nnrTG
Cuts at: 0 61 1236
Size: 61 1175
MspA1I CmG'CkG
Cuts at: 0 123 157 759 967 1236
Size: 123 34 602 208 269
Fragments arranged by size:
602 269 208 123 34
NaeI GCC'GGC
Cuts at: 0 201 1236
Size: 201 1035
NarI GG'CG_CC
Cuts at: 0 224 1236
Size: 224 1012
NcoI C'CATG_G
Cuts at: 0 884 1236
Size: 884 352
NdeI CA'TA_TG
Cuts at: 0 50 1236
Size: 50 1186
NgoMIV G'CCGG_C
Cuts at: 0 199 1236
Size: 199 1037
NotI GC'GGCC_GC
Cuts at: 0 310 1236
Size: 310 926
PasI CC'CwG_GG
Cuts at: 0 378 1216 1236
Size: 378 838 20
Fragments arranged by size:
838 378 20
PfoI T'CCnGG_A
Cuts at: 0 41 1236
Size: 41 1195
PmlI CAC'GTG
Cuts at: 0 694 1236
Size: 694 542
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 1236
Size: 534 32 670
Fragments arranged by size:
670 534 32
PpuMI rG'GwC_Cy
Cuts at: 0 295 1032 1236
Size: 295 737 204
Fragments arranged by size:
737 295 204
PspOMI G'GGCC_C
Cuts at: 0 145 282 1236
Size: 145 137 954
Fragments arranged by size:
954 145 137
PvuII CAG'CTG
Cuts at: 0 759 1236
Size: 759 477
SacI G_AGCT'C
Cuts at: 0 1214 1236
Size: 1214 22
SacII CC_GC'GG
Cuts at: 0 124 1236
Size: 124 1112
SanDI GG'GwC_CC
Cuts at: 0 295 1236
Size: 295 941
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1236
Size: 262 974
SfoI GGC'GCC
Cuts at: 0 225 1236
Size: 225 1011
SmaI CCC'GGG
Cuts at: 0 281 293 1236
Size: 281 12 943
Fragments arranged by size:
943 281 12
SmlI C'TyrA_G
Cuts at: 0 820 1236
Size: 820 416
SrfI GCCC'GGGC
Cuts at: 0 281 1236
Size: 281 955
StuI AGG'CCT
Cuts at: 0 845 1236
Size: 845 391
StyI C'CwwG_G
Cuts at: 0 443 449 884 1127 1236
Size: 443 6 435 243 109
Fragments arranged by size:
443 435 243 109 6
TatI w'GTAC_w
Cuts at: 0 824 1236
Size: 824 412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1047 1236
Size: 1047 189
XmaI C'CCGG_G
Cuts at: 0 279 291 1236
Size: 279 12 945
Fragments arranged by size:
945 279 12
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BcgI BglI BglII
BlpI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtsI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NotI PasI PfoI PmlI PpiI
PpuMI PspOMI PvuII SacI SacII SanDI SapI SfoI
SmaI SmlI SrfI StuI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BmrI BmtI BplI Bpu10I
BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NheI NruI NsiI NspI PacI PciI PflMI
PmeI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TaqII TstI Tth111I
XbaI XhoI XmnI ZraI