Restriction map for
pFN21AE0555
(Linear) (Six-base) MAPSORT of: KIEE0555.seq Check: 5722 from: 1 to: 1131
KIEE0555.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AccI GT'mk_AC
Cuts at: 0 803 1131
Size: 803 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 515 859 1131
Size: 445 70 344 272
Fragments arranged by size:
445 344 272 70
AfeI AGC'GCT
Cuts at: 0 91 115 1131
Size: 91 24 1016
Fragments arranged by size:
1016 91 24
AflII C'TTAA_G
Cuts at: 0 414 1131
Size: 414 717
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 327 359 1131
Size: 327 32 772
Fragments arranged by size:
772 327 32
AlwNI CAG_nnn'CTG
Cuts at: 0 493 1131
Size: 493 638
ApoI r'AATT_y
Cuts at: 0 851 1007 1131
Size: 851 156 124
Fragments arranged by size:
851 156 124
AvaI C'yCGr_G
Cuts at: 0 127 1131
Size: 127 1004
BanII G_rGCy'C
Cuts at: 0 459 1131
Size: 459 672
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 792 1131
Size: 260 532 339
Fragments arranged by size:
532 339 260
BfrBI ATG'CAT
Cuts at: 0 964 1131
Size: 964 167
BglII A'GATC_T
Cuts at: 0 1065 1131
Size: 1065 66
BlpI GC'TnA_GC
Cuts at: 0 441 1131
Size: 441 690
BmtI G_CTAG'C
Cuts at: 0 28 1131
Size: 28 1103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1131
Size: 82 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 887 1131
Size: 471 416 244
Fragments arranged by size:
471 416 244
BsaAI yAC'GTr
Cuts at: 0 75 1131
Size: 75 1056
BsaBI GATnn'nnATC
Cuts at: 0 1070 1131
Size: 1070 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 468 498 1131
Size: 468 30 633
Fragments arranged by size:
633 468 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1131
Size: 506 625
BsmBI CGTCTCn'nnnn_
Cuts at: 0 401 1131
Size: 401 730
Bsp1286I G_dGCh'C
Cuts at: 0 459 1131
Size: 459 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 228 1131
Size: 128 100 903
Fragments arranged by size:
903 128 100
BsrBI CCG'CTC
Cuts at: 0 35 48 1131
Size: 35 13 1083
Fragments arranged by size:
1083 35 13
BsrDI GCAATG_nn'
Cuts at: 0 362 374 677 1131
Size: 362 12 303 454
Fragments arranged by size:
454 362 303 12
BsrGI T'GTAC_A
Cuts at: 0 1116 1131
Size: 1116 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 157 1131
Size: 157 974
BstYI r'GATC_y
Cuts at: 0 974 1065 1131
Size: 974 91 66
Fragments arranged by size:
974 91 66
BtsI GCAGTG_nn'
Cuts at: 0 507 1131
Size: 507 624
EaeI y'GGCC_r
Cuts at: 0 5 357 934 1131
Size: 5 352 577 197
Fragments arranged by size:
577 352 197 5
EarI CTCTTCn'nnn_
Cuts at: 0 297 1131
Size: 297 834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 445 515 859 1131
Size: 82 363 70 344 272
Fragments arranged by size:
363 344 272 82 70
EcoO109I rG'GnC_Cy
Cuts at: 0 240 1131
Size: 240 891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 928 1131
Size: 928 203
HaeII r_GCGC'y
Cuts at: 0 93 117 1131
Size: 93 24 1014
Fragments arranged by size:
1014 93 24
HincII GTy'rAC
Cuts at: 0 143 1131
Size: 143 988
HindIII A'AGCT_T
Cuts at: 0 434 841 1131
Size: 434 407 290
Fragments arranged by size:
434 407 290
MfeI C'AATT_G
Cuts at: 0 146 1131
Size: 146 985
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 1131
Size: 161 970
MscI TGG'CCA
Cuts at: 0 359 936 1131
Size: 359 577 195
Fragments arranged by size:
577 359 195
MslI CAynn'nnrTG
Cuts at: 0 347 367 574 950 1131
Size: 347 20 207 376 181
Fragments arranged by size:
376 347 207 181 20
MspA1I CmG'CkG
Cuts at: 0 465 1131
Size: 465 666
NheI G'CTAG_C
Cuts at: 0 24 1131
Size: 24 1107
NsiI A_TGCA'T
Cuts at: 0 966 1131
Size: 966 165
NspI r_CATG'y
Cuts at: 0 516 964 1131
Size: 516 448 167
Fragments arranged by size:
516 448 167
PfoI T'CCnGG_A
Cuts at: 0 330 1131
Size: 330 801
PmlI CAC'GTG
Cuts at: 0 75 1131
Size: 75 1056
PpuMI rG'GwC_Cy
Cuts at: 0 240 1131
Size: 240 891
PstI C_TGCA'G
Cuts at: 0 239 1131
Size: 239 892
PvuII CAG'CTG
Cuts at: 0 465 1131
Size: 465 666
SapI GCTCTTCn'nnn_
Cuts at: 0 297 1131
Size: 297 834
SbfI CC_TGCA'GG
Cuts at: 0 239 1131
Size: 239 892
SfcI C'TryA_G
Cuts at: 0 235 800 1131
Size: 235 565 331
Fragments arranged by size:
565 331 235
SmlI C'TyrA_G
Cuts at: 0 127 414 450 902 1131
Size: 127 287 36 452 229
Fragments arranged by size:
452 287 229 127 36
SphI G_CATG'C
Cuts at: 0 964 1131
Size: 964 167
StyI C'CwwG_G
Cuts at: 0 1088 1131
Size: 1088 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1131
Size: 813 318
TatI w'GTAC_w
Cuts at: 0 524 1116 1131
Size: 524 592 15
Fragments arranged by size:
592 524 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 468 500 1131
Size: 468 32 631
Fragments arranged by size:
631 468 32
XbaI T'CTAG_A
Cuts at: 0 1062 1131
Size: 1062 69
XhoI C'TCGA_G
Cuts at: 0 127 1131
Size: 127 1004
XmnI GAAnn'nnTTC
Cuts at: 0 1058 1131
Size: 1058 73
Enzymes that do cut:
AccI AcuI AfeI AflII AloI AlwNI ApoI AvaI
BanII BbsI BfrBI BglII BlpI BmtI BpmI BpuEI
BsaAI BsaBI BsaXI BsgI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrGI BstAPI BstYI BtsI EaeI EarI Eco57MI
EcoO109I FalI HaeII HincII HindIII MfeI MmeI MscI
MslI MspA1I NheI NsiI NspI PfoI PmlI PpuMI
PstI PvuII SapI SbfI SfcI SmlI SphI StyI
TaqII TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI
Bme1580I BmgBI BmrI BplI Bpu10I BsaI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HpaI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XcmI XmaI ZraI