(Linear) (Six-base) MAPSORT of: KIEE0532.seq  Check: 7212  from: 1  to: 462



KIEE0532.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AflIII A'CryG_T

Cuts at:      0     381     462 
   Size:        381      81
 
AleI CACnn'nnGTG

Cuts at:      0     124     462 
   Size:        124     338
 
ApaLI G'TGCA_C

Cuts at:      0     217     462 
   Size:        217     245
 
BanI G'GyrC_C

Cuts at:      0     204     462 
   Size:        204     258
 
BanII G_rGCy'C

Cuts at:      0     182     462 
   Size:        182     280
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     198     232     462 
   Size:        198      34     230

  Fragments arranged by size: 

                 230     198      34
 
Bme1580I G_kGCm'C

Cuts at:      0     221     462 
   Size:        221     241
 
BsaHI Gr'CG_yC

Cuts at:      0      92     462 
   Size:         92     370
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     221     462 
   Size:        182      39     241

  Fragments arranged by size: 

                 241     182      39
 
BsmI GAATG_Cn'

Cuts at:      0     291     462 
   Size:        291     171
 
Bsp1286I G_dGCh'C

Cuts at:      0     182     221     462 
   Size:        182      39     241

  Fragments arranged by size: 

                 241     182      39
 
BsrBI CCG'CTC

Cuts at:      0      89     462 
   Size:         89     373
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      34     462 
   Size:         34     428
 
BtgI C'CryG_G

Cuts at:      0      19     462 
   Size:         19     443
 
EaeI y'GGCC_r

Cuts at:      0     450     462 
   Size:        450      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      85     462 
   Size:         85     377
 
EcoICRI GAG'CTC

Cuts at:      0     180     462 
   Size:        180     282
 
HaeII r_GCGC'y

Cuts at:      0     415     462 
   Size:        415      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     418     450     462 
   Size:        418      32      12

  Fragments arranged by size: 

                 418      32      12
 
MscI TGG'CCA

Cuts at:      0     452     462 
   Size:        452      10
 
MslI CAynn'nnrTG

Cuts at:      0     124     241     462 
   Size:        124     117     221

  Fragments arranged by size: 

                 221     124     117
 
MspA1I CmG'CkG

Cuts at:      0      21     231     375     462 
   Size:         21     210     144      87

  Fragments arranged by size: 

                 210     144      87      21
 
NspI r_CATG'y

Cuts at:      0     385     462 
   Size:        385      77
 
PasI CC'CwG_GG

Cuts at:      0     305     462 
   Size:        305     157
 
PciI A'CATG_T

Cuts at:      0     381     462 
   Size:        381      81
 
PvuII CAG'CTG

Cuts at:      0     231     462 
   Size:        231     231
 
RsrII CG'GwC_CG

Cuts at:      0      23     462 
   Size:         23     439
 
SacI G_AGCT'C

Cuts at:      0     182     462 
   Size:        182     280
 
SacII CC_GC'GG

Cuts at:      0      22     462 
   Size:         22     440
 
StyI C'CwwG_G

Cuts at:      0     415     462 
   Size:        415      47
 

 Enzymes that do cut:

   AflIII     AleI    ApaLI     BanI    BanII     BcgI Bme1580I    BsaHI
  BsiHKAI     BsmI Bsp1286I    BsrBI   BstAPI     BtgI     EaeI     EciI
  EcoICRI    HaeII    Hin4I     MscI     MslI   MspA1I     NspI     PasI
     PciI    PvuII    RsrII     SacI    SacII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI