Restriction map for
pFN21AE0500
(Linear) (Six-base) MAPSORT of: KIEE0500.seq Check: 5348 from: 1 to: 2175
KIEE0500.orf
With 182 enzymes: SgfI *
February 17, 2010 19:07 ..
AatII G_ACGT'C
Cuts at: 0 1142 2175
Size: 1142 1033
AccI GT'mk_AC
Cuts at: 0 971 2175
Size: 971 1204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 1171 1588 2175
Size: 4 1167 417 587
Fragments arranged by size:
1167 587 417 4
AflIII A'CryG_T
Cuts at: 0 1412 2175
Size: 1412 763
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1400 2175
Size: 1400 775
AlwNI CAG_nnn'CTG
Cuts at: 0 472 1361 1641 2175
Size: 472 889 280 534
Fragments arranged by size:
889 534 472 280
ApaI G_GGCC'C
Cuts at: 0 1587 1781 2175
Size: 1587 194 394
Fragments arranged by size:
1587 394 194
ApoI r'AATT_y
Cuts at: 0 296 691 712 2175
Size: 296 395 21 1463
Fragments arranged by size:
1463 395 296 21
AvaI C'yCGr_G
Cuts at: 0 1133 2175
Size: 1133 1042
BanI G'GyrC_C
Cuts at: 0 145 2175
Size: 145 2030
BanII G_rGCy'C
Cuts at: 0 660 1587 1768 1781 1955 1994 2175
Size: 660 927 181 13 174 39 181
Fragments arranged by size:
927 660 181 181 174 39 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 1134 1874 2175
Size: 310 824 740 301
Fragments arranged by size:
824 740 310 301
BbvCI CC'TCA_GC
Cuts at: 0 1642 2175
Size: 1642 533
BfrBI ATG'CAT
Cuts at: 0 1105 1452 2175
Size: 1105 347 723
Fragments arranged by size:
1105 723 347
BglII A'GATC_T
Cuts at: 0 1208 1473 2175
Size: 1208 265 702
Fragments arranged by size:
1208 702 265
BlpI GC'TnA_GC
Cuts at: 0 1762 2175
Size: 1762 413
Bme1580I G_kGCm'C
Cuts at: 0 1587 1781 2175
Size: 1587 194 394
Fragments arranged by size:
1587 394 194
BmrI ACTGGGnnnn_n'
Cuts at: 0 696 1775 2175
Size: 696 1079 400
Fragments arranged by size:
1079 696 400
BmtI G_CTAG'C
Cuts at: 0 2144 2175
Size: 2144 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1285 1951 2047 2175
Size: 931 354 666 96 128
Fragments arranged by size:
931 666 354 128 96
Bpu10I CC'TnA_GC
Cuts at: 0 371 659 1642 2175
Size: 371 288 983 533
Fragments arranged by size:
983 533 371 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1939 2008 2175
Size: 1939 69 167
Fragments arranged by size:
1939 167 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 1073 2023 2175
Size: 46 1027 950 152
Fragments arranged by size:
1027 950 152 46
BsaHI Gr'CG_yC
Cuts at: 0 1139 2175
Size: 1139 1036
BsaWI w'CCGG_w
Cuts at: 0 1169 2175
Size: 1169 1006
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1864 1894 2175
Size: 1864 30 281
Fragments arranged by size:
1864 281 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 853 1885 1948 2175
Size: 514 339 1032 63 227
Fragments arranged by size:
1032 514 339 227 63
BseYI C'CCAG_C
Cuts at: 0 2063 2175
Size: 2063 112
BsiHKAI G_wGCw'C
Cuts at: 0 660 770 1955 1994 2175
Size: 660 110 1185 39 181
Fragments arranged by size:
1185 660 181 110 39
BsmI GAATG_Cn'
Cuts at: 0 922 1107 1454 2175
Size: 922 185 347 721
Fragments arranged by size:
922 721 347 185
Bsp1286I G_dGCh'C
Cuts at: 0 660 770 1587 1768 1781 1955 1994 2175
Size: 660 110 817 181 13 174 39 181
Fragments arranged by size:
817 660 181 181 174 110 39 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1241 2175
Size: 1241 934
BsrBI CCG'CTC
Cuts at: 0 329 1406 2175
Size: 329 1077 769
Fragments arranged by size:
1077 769 329
BsrFI r'CCGG_y
Cuts at: 0 1517 2175
Size: 1517 658
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1284 1361 2175
Size: 1284 77 814
Fragments arranged by size:
1284 814 77
BstBI TT'CG_AA
Cuts at: 0 321 958 2175
Size: 321 637 1217
Fragments arranged by size:
1217 637 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2127 2175
Size: 2127 48
BstYI r'GATC_y
Cuts at: 0 53 280 1115 1208 1473 2175
Size: 53 227 835 93 265 702
Fragments arranged by size:
835 702 265 227 93 53
BtgI C'CryG_G
Cuts at: 0 5 1124 2175
Size: 5 1119 1051
Fragments arranged by size:
1119 1051 5
BtsI GCAGTG_nn'
Cuts at: 0 473 2175
Size: 473 1702
DraI TTT'AAA
Cuts at: 0 1727 2175
Size: 1727 448
EaeI y'GGCC_r
Cuts at: 0 2 818 1041 2117 2175
Size: 2 816 223 1076 58
Fragments arranged by size:
1076 816 223 58 2
EarI CTCTTCn'nnn_
Cuts at: 0 503 543 611 646 758 1808 1862 1943
Size: 503 40 68 35 112 1050 54 81
Cuts at: 1943 2126 2175
Size: 183 49
Fragments arranged by size:
1050 503 183 112 81 68 54 49
40 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1091 2175
Size: 1091 1084
EcoICRI GAG'CTC
Cuts at: 0 658 1953 1992 2175
Size: 658 1295 39 183
Fragments arranged by size:
1295 658 183 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 931 1171 1285 1588 1951 2047 2175
Size: 4 927 240 114 303 363 96 128
Fragments arranged by size:
927 363 303 240 128 114 96 4
EcoO109I rG'GnC_Cy
Cuts at: 0 1583 1584 2175
Size: 1583 1 591
Fragments arranged by size:
1583 591 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 1575 2175
Size: 334 1241 600
Fragments arranged by size:
1241 600 334
HaeII r_GCGC'y
Cuts at: 0 1361 1843 2175
Size: 1361 482 332
Fragments arranged by size:
1361 482 332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1864 1896 2084 2116 2175
Size: 1864 32 188 32 59
Fragments arranged by size:
1864 188 59 32 32
HincII GTy'rAC
Cuts at: 0 288 1317 2175
Size: 288 1029 858
Fragments arranged by size:
1029 858 288
HpaI GTT'AAC
Cuts at: 0 1317 2175
Size: 1317 858
MluI A'CGCG_T
Cuts at: 0 1412 2175
Size: 1412 763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 2175
Size: 81 2094
MscI TGG'CCA
Cuts at: 0 4 820 1043 2119 2175
Size: 4 816 223 1076 56
Fragments arranged by size:
1076 816 223 56 4
MslI CAynn'nnrTG
Cuts at: 0 337 388 1339 2175
Size: 337 51 951 836
Fragments arranged by size:
951 836 337 51
MspA1I CmG'CkG
Cuts at: 0 406 2127 2175
Size: 406 1721 48
Fragments arranged by size:
1721 406 48
NcoI C'CATG_G
Cuts at: 0 5 1124 2175
Size: 5 1119 1051
Fragments arranged by size:
1119 1051 5
NdeI CA'TA_TG
Cuts at: 0 1038 2175
Size: 1038 1137
NheI G'CTAG_C
Cuts at: 0 2140 2175
Size: 2140 35
NsiI A_TGCA'T
Cuts at: 0 1107 1454 2175
Size: 1107 347 721
Fragments arranged by size:
1107 721 347
NspI r_CATG'y
Cuts at: 0 342 2175
Size: 342 1833
PasI CC'CwG_GG
Cuts at: 0 1587 2175
Size: 1587 588
PspOMI G'GGCC_C
Cuts at: 0 1583 1777 2175
Size: 1583 194 398
Fragments arranged by size:
1583 398 194
PstI C_TGCA'G
Cuts at: 0 405 468 1304 1366 2175
Size: 405 63 836 62 809
Fragments arranged by size:
836 809 405 63 62
PvuII CAG'CTG
Cuts at: 0 406 2127 2175
Size: 406 1721 48
Fragments arranged by size:
1721 406 48
SacI G_AGCT'C
Cuts at: 0 660 1955 1994 2175
Size: 660 1295 39 181
Fragments arranged by size:
1295 660 181 39
SapI GCTCTTCn'nnn_
Cuts at: 0 503 543 758 1943 2126 2175
Size: 503 40 215 1185 183 49
Fragments arranged by size:
1185 503 215 183 49 40
SfcI C'TryA_G
Cuts at: 0 401 464 972 1300 1362 2175
Size: 401 63 508 328 62 813
Fragments arranged by size:
813 508 401 328 63 62
SmaI CCC'GGG
Cuts at: 0 1135 2175
Size: 1135 1040
SmlI C'TyrA_G
Cuts at: 0 1954 1987 2175
Size: 1954 33 188
Fragments arranged by size:
1954 188 33
StuI AGG'CCT
Cuts at: 0 453 2107 2175
Size: 453 1654 68
Fragments arranged by size:
1654 453 68
StyI C'CwwG_G
Cuts at: 0 5 1124 1862 2175
Size: 5 1119 738 313
Fragments arranged by size:
1119 738 313 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2020 2175
Size: 2020 155
Tth111I GACn'n_nGTC
Cuts at: 0 1750 2175
Size: 1750 425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 64 888 1051 2175
Size: 64 824 163 1124
Fragments arranged by size:
1124 824 163 64
XmaI C'CCGG_G
Cuts at: 0 1133 2175
Size: 1133 1042
XmnI GAAnn'nnTTC
Cuts at: 0 317 2175
Size: 317 1858
ZraI GAC'GTC
Cuts at: 0 1140 2175
Size: 1140 1035
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BfrBI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstBI BstXI BstYI BtgI BtsI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HpaI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NsiI NspI PasI PspOMI
PstI PvuII SacI SapI SfcI SmaI SmlI StuI
StyI TaqII Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BglI BmgBI BplI BsaAI
BsaBI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MfeI NaeI NarI
NgoMIV NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XhoI