(Linear) (Six-base) MAPSORT of: KIEE0499.seq  Check: 5095  from: 1  to: 2037



KIEE0499.orf

 With 182 enzymes: SgfI * 

                        February 17, 2010 19:07  ..

Acc65I G'GTAC_C

Cuts at:      0     719    2037 
   Size:        719    1318
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1886    2037 
   Size:       1886     151
 
AleI CACnn'nnGTG

Cuts at:      0    1450    2037 
   Size:       1450     587
 
ApaLI G'TGCA_C

Cuts at:      0     269    2037 
   Size:        269    1768
 
ApoI r'AATT_y

Cuts at:      0     361     929    1022    1273    2037 
   Size:        361     568      93     251     764

  Fragments arranged by size: 

                 764     568     361     251      93
 
AseI AT'TA_AT

Cuts at:      0    1361    2037 
   Size:       1361     676
 
AvaI C'yCGr_G

Cuts at:      0     947    2037 
   Size:        947    1090
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1470    1503    2037 
   Size:       1470      33     534

  Fragments arranged by size: 

                1470     534      33
 
BanI G'GyrC_C

Cuts at:      0     719    2037 
   Size:        719    1318
 
BanII G_rGCy'C

Cuts at:      0     474    2037 
   Size:        474    1563
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290    2037 
   Size:        290    1747
 
BbvCI CC'TCA_GC

Cuts at:      0     171    2037 
   Size:        171    1866
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1876    1910    2037 
   Size:       1876      34     127

  Fragments arranged by size: 

                1876     127      34
 
BclI T'GATC_A

Cuts at:      0     518    2037 
   Size:        518    1519
 
BfrBI ATG'CAT

Cuts at:      0     937    2037 
   Size:        937    1100
 
BglII A'GATC_T

Cuts at:      0     642    2037 
   Size:        642    1395
 
Bme1580I G_kGCm'C

Cuts at:      0     273    2034    2037 
   Size:        273    1761       3

  Fragments arranged by size: 

                1761     273       3
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     134     272    2037 
   Size:        134     138    1765

  Fragments arranged by size: 

                1765     138     134
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1694    2037 
   Size:       1694     343
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     146     323    1496    2037 
   Size:        106      40     177    1173     541

  Fragments arranged by size: 

                1173     541     177     106      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     171    2037 
   Size:        171    1866
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1366    1939    2037 
   Size:       1366     573      98

  Fragments arranged by size: 

                1366     573      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2016    2037 
   Size:       2016      21
 
BsaAI yAC'GTr

Cuts at:      0     268     379    2037 
   Size:        268     111    1658

  Fragments arranged by size: 

                1658     268     111
 
BsaWI w'CCGG_w

Cuts at:      0    1186    2037 
   Size:       1186     851
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1494    1524    2037 
   Size:       1494      30     513

  Fragments arranged by size: 

                1494     513      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1322    2002    2037 
   Size:       1322     680      35

  Fragments arranged by size: 

                1322     680      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290    1994    2037 
   Size:        290    1704      43

  Fragments arranged by size: 

                1704     290      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     273    2037 
   Size:        273    1764
 
Bsp1286I G_dGCh'C

Cuts at:      0     273     474    2034    2037 
   Size:        273     201    1560       3

  Fragments arranged by size: 

                1560     273     201       3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1086    1498    2037 
   Size:       1086     412     539

  Fragments arranged by size: 

                1086     539     412
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1175    2037 
   Size:       1175     862
 
BstYI r'GATC_y

Cuts at:      0     567     642    1584    2037 
   Size:        567      75     942     453

  Fragments arranged by size: 

                 942     567     453      75
 
Bsu36I CC'TnA_GG

Cuts at:      0    1401    2037 
   Size:       1401     636
 
BtsI GCAGTG_nn'

Cuts at:      0    1235    1814    2037 
   Size:       1235     579     223

  Fragments arranged by size: 

                1235     579     223
 
DraI TTT'AAA

Cuts at:      0      14    2037 
   Size:         14    2023
 
EarI CTCTTCn'nnn_

Cuts at:      0    2014    2037 
   Size:       2014      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     146     323    1496    1886    2037 
   Size:        106      40     177    1173     390     151

  Fragments arranged by size: 

                1173     390     177     151     106      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1848    2037 
   Size:       1848     189
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1481    1869    1933    2037 
   Size:       1481     388      64     104

  Fragments arranged by size: 

                1481     388     104      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1569    1601    1674    1706    2037 
   Size:       1569      32      73      32     331

  Fragments arranged by size: 

                1569     331      73      32      32
 
KpnI G_GTAC'C

Cuts at:      0     723    2037 
   Size:        723    1314
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1612    2037 
   Size:       1612     425
 
MslI CAynn'nnrTG

Cuts at:      0    1450    2037 
   Size:       1450     587
 
NdeI CA'TA_TG

Cuts at:      0    1893    2037 
   Size:       1893     144
 
NsiI A_TGCA'T

Cuts at:      0     939    2037 
   Size:        939    1098
 
NspI r_CATG'y

Cuts at:      0     845    2037 
   Size:        845    1192
 
PfoI T'CCnGG_A

Cuts at:      0    1512    1814    2037 
   Size:       1512     302     223

  Fragments arranged by size: 

                1512     302     223
 
PmlI CAC'GTG

Cuts at:      0     268    2037 
   Size:        268    1769
 
SfcI C'TryA_G

Cuts at:      0     209    1580    2037 
   Size:        209    1371     457

  Fragments arranged by size: 

                1371     457     209
 
SmlI C'TyrA_G

Cuts at:      0     947    1381    1918    2037 
   Size:        947     434     537     119

  Fragments arranged by size: 

                 947     537     434     119
 
SpeI A'CTAG_T

Cuts at:      0     807    2037 
   Size:        807    1230
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     877    2037 
   Size:        877    1160
 
TatI w'GTAC_w

Cuts at:      0    1146    2037 
   Size:       1146     891
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1494    1526    2037 
   Size:       1494      32     511

  Fragments arranged by size: 

                1494     511      32
 
XbaI T'CTAG_A

Cuts at:      0    1077    2037 
   Size:       1077     960
 
XhoI C'TCGA_G

Cuts at:      0     947    2037 
   Size:        947    1090
 
XmnI GAAnn'nnTTC

Cuts at:      0    1823    1913    1966    2037 
   Size:       1823      90      53      71

  Fragments arranged by size: 

                1823      90      71      53
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI    ApaLI     ApoI     AseI     AvaI     BaeI
     BanI    BanII     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstYI   Bsu36I     BtsI     DraI     EarI  Eco57MI EcoO109I     FalI
    Hin4I     KpnI     MmeI     MslI     NdeI     NsiI     NspI     PfoI
     PmlI     SfcI     SmlI     SpeI    TaqII     TatI     TstI     XbaI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI    AsiSI    AvrII
    BamHI     BbeI    BciVI     BglI     BlpI    BmgBI     BmtI    BsaBI
    BsaHI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     StyI     SwaI  Tth111I     XcmI
     XmaI     ZraI