Restriction map for
pFN21AE0497
(Linear) (Six-base) MAPSORT of: KIEE0497.seq Check: 7955 from: 1 to: 1200
KIEE0497.orf
With 182 enzymes: SgfI *
February 2, 2010 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 992 1200
Size: 992 208
AatII G_ACGT'C
Cuts at: 0 953 1200
Size: 953 247
AccI GT'mk_AC
Cuts at: 0 311 1200
Size: 311 889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 370 832 946 1200
Size: 232 138 462 114 254
Fragments arranged by size:
462 254 232 138 114
AflIII A'CryG_T
Cuts at: 0 566 1200
Size: 566 634
AleI CACnn'nnGTG
Cuts at: 0 680 706 1200
Size: 680 26 494
Fragments arranged by size:
680 494 26
AlwNI CAG_nnn'CTG
Cuts at: 0 477 744 1166 1200
Size: 477 267 422 34
Fragments arranged by size:
477 422 267 34
ApaI G_GGCC'C
Cuts at: 0 364 1200
Size: 364 836
ApaLI G'TGCA_C
Cuts at: 0 709 1118 1200
Size: 709 409 82
Fragments arranged by size:
709 409 82
AvrII C'CTAG_G
Cuts at: 0 1143 1200
Size: 1143 57
BamHI G'GATC_C
Cuts at: 0 1011 1200
Size: 1011 189
BanII G_rGCy'C
Cuts at: 0 364 515 557 1200
Size: 364 151 42 643
Fragments arranged by size:
643 364 151 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1020 1054 1200
Size: 1020 34 146
Fragments arranged by size:
1020 146 34
BlpI GC'TnA_GC
Cuts at: 0 1126 1200
Size: 1126 74
Bme1580I G_kGCm'C
Cuts at: 0 364 713 1122 1200
Size: 364 349 409 78
Fragments arranged by size:
409 364 349 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 759 924 1200
Size: 759 165 276
Fragments arranged by size:
759 276 165
Bpu10I CC'TnA_GC
Cuts at: 0 418 1200
Size: 418 782
BsaAI yAC'GTr
Cuts at: 0 612 1200
Size: 612 588
BsaHI Gr'CG_yC
Cuts at: 0 950 1200
Size: 950 250
BsaWI w'CCGG_w
Cuts at: 0 50 287 1200
Size: 50 237 913
Fragments arranged by size:
913 237 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 1200
Size: 217 30 953
Fragments arranged by size:
953 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 151 241 262 537 952 1108 1200
Size: 79 72 90 21 275 415 156 92
Fragments arranged by size:
415 275 156 92 90 79 72 21
BseYI C'CCAG_C
Cuts at: 0 689 975 1200
Size: 689 286 225
Fragments arranged by size:
689 286 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1101 1152 1200
Size: 502 599 51 48
Fragments arranged by size:
599 502 51 48
BsiEI CG_ry'CG
Cuts at: 0 764 871 1200
Size: 764 107 329
Fragments arranged by size:
764 329 107
BsiHKAI G_wGCw'C
Cuts at: 0 557 713 1122 1200
Size: 557 156 409 78
Fragments arranged by size:
557 409 156 78
Bsp1286I G_dGCh'C
Cuts at: 0 364 515 557 713 1122 1200
Size: 364 151 42 156 409 78
Fragments arranged by size:
409 364 156 151 78 42
BspEI T'CCGG_A
Cuts at: 0 50 287 1200
Size: 50 237 913
Fragments arranged by size:
913 237 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 679 899 992 1200
Size: 679 220 93 208
Fragments arranged by size:
679 220 208 93
BsrBI CCG'CTC
Cuts at: 0 23 45 1200
Size: 23 22 1155
Fragments arranged by size:
1155 23 22
BsrFI r'CCGG_y
Cuts at: 0 143 194 863 1200
Size: 143 51 669 337
Fragments arranged by size:
669 337 143 51
BsrGI T'GTAC_A
Cuts at: 0 569 1200
Size: 569 631
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1166 1200
Size: 1166 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 303 682 839 1200
Size: 303 379 157 361
Fragments arranged by size:
379 361 303 157
BstYI r'GATC_y
Cuts at: 0 986 1011 1200
Size: 986 25 189
Fragments arranged by size:
986 189 25
Bsu36I CC'TnA_GG
Cuts at: 0 106 520 1200
Size: 106 414 680
Fragments arranged by size:
680 414 106
BtgI C'CryG_G
Cuts at: 0 558 1200
Size: 558 642
BtsI GCAGTG_nn'
Cuts at: 0 379 638 901 1200
Size: 379 259 263 299
Fragments arranged by size:
379 299 263 259
EaeI y'GGCC_r
Cuts at: 0 152 275 542 868 968 1200
Size: 152 123 267 326 100 232
Fragments arranged by size:
326 267 232 152 123 100
EagI C'GGCC_G
Cuts at: 0 868 1200
Size: 868 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 76 136 1200
Size: 55 21 60 1064
Fragments arranged by size:
1064 60 55 21
EcoICRI GAG'CTC
Cuts at: 0 555 1200
Size: 555 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 370 832 946 1200
Size: 232 138 462 114 254
Fragments arranged by size:
462 254 232 138 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 88 109 421 999 1200
Size: 88 21 312 578 201
Fragments arranged by size:
578 312 201 88 21
EcoO109I rG'GnC_Cy
Cuts at: 0 207 360 361 686 1098 1200
Size: 207 153 1 325 412 102
Fragments arranged by size:
412 325 207 153 102 1
HaeII r_GCGC'y
Cuts at: 0 84 134 284 1200
Size: 84 50 150 916
Fragments arranged by size:
916 150 84 50
HindIII A'AGCT_T
Cuts at: 0 874 1200
Size: 874 326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1200
Size: 1048 152
MscI TGG'CCA
Cuts at: 0 277 1200
Size: 277 923
MslI CAynn'nnrTG
Cuts at: 0 445 680 706 1200
Size: 445 235 26 494
Fragments arranged by size:
494 445 235 26
MspA1I CmG'CkG
Cuts at: 0 693 1200
Size: 693 507
NaeI GCC'GGC
Cuts at: 0 145 196 865 1200
Size: 145 51 669 335
Fragments arranged by size:
669 335 145 51
NgoMIV G'CCGG_C
Cuts at: 0 143 194 863 1200
Size: 143 51 669 337
Fragments arranged by size:
669 337 143 51
NspI r_CATG'y
Cuts at: 0 490 570 1200
Size: 490 80 630
Fragments arranged by size:
630 490 80
PciI A'CATG_T
Cuts at: 0 566 1200
Size: 566 634
PfoI T'CCnGG_A
Cuts at: 0 266 1200
Size: 266 934
PpuMI rG'GwC_Cy
Cuts at: 0 686 1200
Size: 686 514
PspOMI G'GGCC_C
Cuts at: 0 360 1200
Size: 360 840
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 283 315 1200
Size: 283 32 885
Fragments arranged by size:
885 283 32
PstI C_TGCA'G
Cuts at: 0 185 896 1200
Size: 185 711 304
Fragments arranged by size:
711 304 185
PvuII CAG'CTG
Cuts at: 0 693 1200
Size: 693 507
SacI G_AGCT'C
Cuts at: 0 557 1200
Size: 557 643
SanDI GG'GwC_CC
Cuts at: 0 686 1200
Size: 686 514
SexAI A'CCwGG_T
Cuts at: 0 167 1200
Size: 167 1033
SfcI C'TryA_G
Cuts at: 0 181 892 996 1200
Size: 181 711 104 204
Fragments arranged by size:
711 204 181 104
StyI C'CwwG_G
Cuts at: 0 1143 1200
Size: 1143 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 457 778 1200
Size: 457 321 422
Fragments arranged by size:
457 422 321
TatI w'GTAC_w
Cuts at: 0 569 1200
Size: 569 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 1200
Size: 217 32 951
Fragments arranged by size:
951 217 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 523 1023 1200
Size: 523 500 177
Fragments arranged by size:
523 500 177
ZraI GAC'GTC
Cuts at: 0 951 1200
Size: 951 249
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlwNI ApaI
ApaLI AvrII BamHI BanII BcgI BlpI Bme1580I BmrI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII HindIII MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PciI PfoI
PpuMI PspOMI PsrI PstI PvuII SacI SanDI SexAI
SfcI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvaI BaeI BanI BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI BsmI
BsmBI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI