(Linear) (Six-base) MAPSORT of: KIEE0480.seq  Check: 4351  from: 1  to: 1425



KIEE0480.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AccI GT'mk_AC

Cuts at:      0    1358    1425 
   Size:       1358      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     455     836    1425 
   Size:        333     122     381     589

  Fragments arranged by size: 

                 589     381     333     122
 
AflIII A'CryG_T

Cuts at:      0     263    1425 
   Size:        263    1162
 
AhdI GACnn_n'nnGTC

Cuts at:      0     821    1425 
   Size:        821     604
 
ApaLI G'TGCA_C

Cuts at:      0     520    1425 
   Size:        520     905
 
ApoI r'AATT_y

Cuts at:      0      45     121    1061    1117    1348    1425 
   Size:         45      76     940      56     231      77

  Fragments arranged by size: 

                 940     231      77      76      56      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339     817    1425 
   Size:        339     478     608

  Fragments arranged by size: 

                 608     478     339
 
BfrBI ATG'CAT

Cuts at:      0     757    1425 
   Size:        757     668
 
Bme1580I G_kGCm'C

Cuts at:      0     524    1425 
   Size:        524     901
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1425 
   Size:       1031     394
 
BsiHKAI G_wGCw'C

Cuts at:      0     524    1425 
   Size:        524     901
 
BsmI GAATG_Cn'

Cuts at:      0     578     757    1425 
   Size:        578     179     668

  Fragments arranged by size: 

                 668     578     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     524    1425 
   Size:        524     901
 
BspHI T'CATG_A

Cuts at:      0    1074    1425 
   Size:       1074     351
 
BsrGI T'GTAC_A

Cuts at:      0     266     300    1425 
   Size:        266      34    1125

  Fragments arranged by size: 

                1125     266      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1285    1425 
   Size:       1285     140
 
BstZ17I GTA'TAC

Cuts at:      0    1359    1425 
   Size:       1359      66
 
Bsu36I CC'TnA_GG

Cuts at:      0    1337    1425 
   Size:       1337      88
 
BtsI GCAGTG_nn'

Cuts at:      0    1267    1425 
   Size:       1267     158
 
ClaI AT'CG_AT

Cuts at:      0     450    1425 
   Size:        450     975
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1256    1291    1425 
   Size:       1256      35     134

  Fragments arranged by size: 

                1256     134      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     566    1425 
   Size:        566     859
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     455     836    1031    1425 
   Size:        333     122     381     195     394

  Fragments arranged by size: 

                 394     381     333     195     122
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     235    1425 
   Size:        235    1190
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     807     839     895     927    1210    1242    1425 
   Size:        807      32      56      32     283      32     183

  Fragments arranged by size: 

                 807     283     183      56      32      32      32
 
HincII GTy'rAC

Cuts at:      0     741    1425 
   Size:        741     684
 
MfeI C'AATT_G

Cuts at:      0    1242    1273    1425 
   Size:       1242      31     152

  Fragments arranged by size: 

                1242     152      31
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1425 
   Size:        416    1009
 
MslI CAynn'nnrTG

Cuts at:      0     383    1425 
   Size:        383    1042
 
MspA1I CmG'CkG

Cuts at:      0    1114    1425 
   Size:       1114     311
 
NsiI A_TGCA'T

Cuts at:      0     759    1425 
   Size:        759     666
 
NspI r_CATG'y

Cuts at:      0     267    1283    1425 
   Size:        267    1016     142

  Fragments arranged by size: 

                1016     267     142
 
PciI A'CATG_T

Cuts at:      0     263    1425 
   Size:        263    1162
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     138    1425 
   Size:        138    1287
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     954     986    1425 
   Size:        954      32     439

  Fragments arranged by size: 

                 954     439      32
 
PvuII CAG'CTG

Cuts at:      0    1114    1425 
   Size:       1114     311
 
ScaI AGT'ACT

Cuts at:      0    1225    1425 
   Size:       1225     200
 
SfcI C'TryA_G

Cuts at:      0     308    1229    1289    1425 
   Size:        308     921      60     136

  Fragments arranged by size: 

                 921     308     136      60
 
SspI AAT'ATT

Cuts at:      0     463    1425 
   Size:        463     962
 
TatI w'GTAC_w

Cuts at:      0     266     300     970    1223    1425 
   Size:        266      34     670     253     202

  Fragments arranged by size: 

                 670     266     253     202      34
 
XbaI T'CTAG_A

Cuts at:      0    1036    1425 
   Size:       1036     389
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI    ApaLI     ApoI     BbsI    BfrBI
 Bme1580I     BpmI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrGI   BstAPI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     EarI  Eco57MI     FalI
    Hin4I   HincII     MfeI     MmeI     MslI   MspA1I     NsiI     NspI
     PciI    PflMI     PsrI    PvuII     ScaI     SfcI     SspI     TatI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI