Restriction map for
pFN21AE0462
(Linear) (Six-base) MAPSORT of: KIEE0462.seq Check: 1478 from: 1 to: 681
KIEE0462.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AfeI AGC'GCT
Cuts at: 0 118 547 681
Size: 118 429 134
Fragments arranged by size:
429 134 118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 681
Size: 280 401
AlwNI CAG_nnn'CTG
Cuts at: 0 48 681
Size: 48 633
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 153 186 681
Size: 153 33 495
Fragments arranged by size:
495 153 33
BanI G'GyrC_C
Cuts at: 0 76 681
Size: 76 605
BanII G_rGCy'C
Cuts at: 0 89 681
Size: 89 592
BbeI G_GCGC'C
Cuts at: 0 80 681
Size: 80 601
BbsI GAAGACnn'nnnn_
Cuts at: 0 192 681
Size: 192 489
BclI T'GATC_A
Cuts at: 0 492 681
Size: 492 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 681
Size: 634 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 139 681
Size: 100 39 542
Fragments arranged by size:
542 100 39
BsaAI yAC'GTr
Cuts at: 0 234 681
Size: 234 447
BsaHI Gr'CG_yC
Cuts at: 0 77 681
Size: 77 604
BseYI C'CCAG_C
Cuts at: 0 16 681
Size: 16 665
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 681
Size: 325 356
BsiHKAI G_wGCw'C
Cuts at: 0 571 681
Size: 571 110
Bsp1286I G_dGCh'C
Cuts at: 0 89 571 681
Size: 89 482 110
Fragments arranged by size:
482 110 89
BsrDI GCAATG_nn'
Cuts at: 0 596 681
Size: 596 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 48 681
Size: 48 633
BstEII G'GTnAC_C
Cuts at: 0 322 681
Size: 322 359
BstXI CCAn_nnnn'nTGG
Cuts at: 0 675 681
Size: 675 6
BtgI C'CryG_G
Cuts at: 0 149 681
Size: 149 532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 46 681
Size: 46 635
EarI CTCTTCn'nnn_
Cuts at: 0 373 536 681
Size: 373 163 145
Fragments arranged by size:
373 163 145
EcoO109I rG'GnC_Cy
Cuts at: 0 45 246 582 681
Size: 45 201 336 99
Fragments arranged by size:
336 201 99 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 216 675 681
Size: 216 459 6
Fragments arranged by size:
459 216 6
HaeII r_GCGC'y
Cuts at: 0 80 120 549 681
Size: 80 40 429 132
Fragments arranged by size:
429 132 80 40
KasI G'GCGC_C
Cuts at: 0 76 681
Size: 76 605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 681
Size: 576 105
MslI CAynn'nnrTG
Cuts at: 0 232 681
Size: 232 449
NarI GG'CG_CC
Cuts at: 0 77 681
Size: 77 604
NcoI C'CATG_G
Cuts at: 0 149 681
Size: 149 532
NspI r_CATG'y
Cuts at: 0 231 421 681
Size: 231 190 260
Fragments arranged by size:
260 231 190
PasI CC'CwG_GG
Cuts at: 0 81 681
Size: 81 600
PmlI CAC'GTG
Cuts at: 0 234 681
Size: 234 447
PpuMI rG'GwC_Cy
Cuts at: 0 246 582 681
Size: 246 336 99
Fragments arranged by size:
336 246 99
PshAI GACnn'nnGTC
Cuts at: 0 151 681
Size: 151 530
SapI GCTCTTCn'nnn_
Cuts at: 0 536 681
Size: 536 145
SfcI C'TryA_G
Cuts at: 0 466 681
Size: 466 215
SfoI GGC'GCC
Cuts at: 0 78 681
Size: 78 603
SmlI C'TyrA_G
Cuts at: 0 649 681
Size: 649 32
SphI G_CATG'C
Cuts at: 0 231 421 681
Size: 231 190 260
Fragments arranged by size:
260 231 190
StyI C'CwwG_G
Cuts at: 0 149 681
Size: 149 532
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 681
Size: 124 557
XmnI GAAnn'nnTTC
Cuts at: 0 506 681
Size: 506 175
Enzymes that do cut:
AfeI AlfI AlwNI BaeI BanI BanII BbeI BbsI
BclI BpuEI BsaI BsaAI BsaHI BseYI BsgI BsiHKAI
Bsp1286I BsrDI BstAPI BstEII BstXI BtgI BtgZI EarI
EcoO109I FalI HaeII KasI MmeI MslI NarI NcoI
NspI PasI PmlI PpuMI PshAI SapI SfcI SfoI
SmlI SphI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI