(Linear) (Six-base) MAPSORT of: KIEE0453.seq  Check: 3978  from: 1  to: 681



KIEE0453.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

Acc65I G'GTAC_C

Cuts at:      0       9     681 
   Size:          9     672
 
ApoI r'AATT_y

Cuts at:      0     208     453     681 
   Size:        208     245     228

  Fragments arranged by size: 

                 245     228     208
 
AvaI C'yCGr_G

Cuts at:      0      41     681 
   Size:         41     640
 
BanI G'GyrC_C

Cuts at:      0       9     681 
   Size:          9     672
 
BanII G_rGCy'C

Cuts at:      0     155     681 
   Size:        155     526
 
BbvCI CC'TCA_GC

Cuts at:      0     647     681 
   Size:        647      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     482     681 
   Size:        482     199
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394     423     681 
   Size:        394      29     258

  Fragments arranged by size: 

                 394     258      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     681 
   Size:        118     563
 
Bpu10I CC'TnA_GC

Cuts at:      0     647     681 
   Size:        647      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     441     681 
   Size:        441     240
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     243     455     681 
   Size:        243     212     226

  Fragments arranged by size: 

                 243     226     212
 
BseYI C'CCAG_C

Cuts at:      0     294     681 
   Size:        294     387
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     569     681 
   Size:        470      99     112

  Fragments arranged by size: 

                 470     112      99
 
BsiHKAI G_wGCw'C

Cuts at:      0     266     681 
   Size:        266     415
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     266     681 
   Size:        155     111     415

  Fragments arranged by size: 

                 415     155     111
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     650     681 
   Size:        650      31
 
BsrFI r'CCGG_y

Cuts at:      0      31     266     681 
   Size:         31     235     415

  Fragments arranged by size: 

                 415     235      31
 
BsrGI T'GTAC_A

Cuts at:      0     255     681 
   Size:        255     426
 
Bsu36I CC'TnA_GG

Cuts at:      0     503     681 
   Size:        503     178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     681 
   Size:        118     563
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     372     404     681 
   Size:        372      32     277

  Fragments arranged by size: 

                 372     277      32
 
KpnI G_GTAC'C

Cuts at:      0      13     681 
   Size:         13     668
 
MspA1I CmG'CkG

Cuts at:      0     294     681 
   Size:        294     387
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     622     681 
   Size:        622      59
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     230     262     681 
   Size:        230      32     419

  Fragments arranged by size: 

                 419     230      32
 
PstI C_TGCA'G

Cuts at:      0     293     681 
   Size:        293     388
 
PvuII CAG'CTG

Cuts at:      0     294     681 
   Size:        294     387
 
SexAI A'CCwGG_T

Cuts at:      0     318     681 
   Size:        318     363
 
SfcI C'TryA_G

Cuts at:      0     236     289     681 
   Size:        236      53     392

  Fragments arranged by size: 

                 392     236      53
 
SmaI CCC'GGG

Cuts at:      0      43     681 
   Size:         43     638
 
TatI w'GTAC_w

Cuts at:      0      68     255     681 
   Size:         68     187     426

  Fragments arranged by size: 

                 426     187      68
 
Tth111I GACn'n_nGTC

Cuts at:      0     415     681 
   Size:        415     266
 
XmaI C'CCGG_G

Cuts at:      0      41     681 
   Size:         41     640
 
XmnI GAAnn'nnTTC

Cuts at:      0     245     681 
   Size:        245     436
 

 Enzymes that do cut:

   Acc65I     ApoI     AvaI     BanI    BanII    BbvCI    BciVI     BmrI
     BpmI   Bpu10I     BsaI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrFI    BsrGI   Bsu36I  Eco57MI    Hin4I     KpnI   MspA1I
    PflMI     PsrI     PstI    PvuII    SexAI     SfcI     SmaI     TatI
  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI
     ZraI