Restriction map for
pFN21AE0400
(Linear) (Six-base) MAPSORT of: KIEE0400.seq Check: 7223 from: 1 to: 1098
KIEE0400.orf
With 182 enzymes: SgfI *
March 22, 2012 15:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 299 895 1098
Size: 299 596 203
Fragments arranged by size:
596 299 203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1098
Size: 779 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 307 1098
Size: 109 198 791
Fragments arranged by size:
791 198 109
AlwNI CAG_nnn'CTG
Cuts at: 0 81 318 567 588 1098
Size: 81 237 249 21 510
Fragments arranged by size:
510 249 237 81 21
ApaI G_GGCC'C
Cuts at: 0 177 628 1098
Size: 177 451 470
Fragments arranged by size:
470 451 177
AvaI C'yCGr_G
Cuts at: 0 52 428 855 1098
Size: 52 376 427 243
Fragments arranged by size:
427 376 243 52
BanI G'GyrC_C
Cuts at: 0 117 640 675 778 883 1098
Size: 117 523 35 103 105 215
Fragments arranged by size:
523 215 117 105 103 35
BanII G_rGCy'C
Cuts at: 0 177 628 698 741 854 1098
Size: 177 451 70 43 113 244
Fragments arranged by size:
451 244 177 113 70 43
BbeI G_GCGC'C
Cuts at: 0 644 679 1098
Size: 644 35 419
Fragments arranged by size:
644 419 35
BglI GCCn_nnn'nGGC
Cuts at: 0 12 684 1086 1098
Size: 12 672 402 12
Fragments arranged by size:
672 402 12 12
BlpI GC'TnA_GC
Cuts at: 0 109 568 1098
Size: 109 459 530
Fragments arranged by size:
530 459 109
Bme1580I G_kGCm'C
Cuts at: 0 27 122 177 628 781 886 1098
Size: 27 95 55 451 153 105 212
Fragments arranged by size:
451 212 153 105 95 55 27
BmgBI CAC'GTC
Cuts at: 0 537 1098
Size: 537 561
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 849 1098
Size: 171 678 249
Fragments arranged by size:
678 249 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1098
Size: 981 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 724 868 1075 1098
Size: 477 247 144 207 23
Fragments arranged by size:
477 247 207 144 23
Bpu10I CC'TnA_GC
Cuts at: 0 740 1098
Size: 740 358
BsaAI yAC'GTr
Cuts at: 0 168 1098
Size: 168 930
BsaHI Gr'CG_yC
Cuts at: 0 271 634 641 676 1098
Size: 271 363 7 35 422
Fragments arranged by size:
422 363 271 35 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 463 481 493 1098
Size: 451 12 18 12 605
Fragments arranged by size:
605 451 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1098
Size: 85 1013
BseYI C'CCAG_C
Cuts at: 0 20 698 1098
Size: 20 678 400
Fragments arranged by size:
678 400 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 373 628 694 1063 1071 1098
Size: 361 12 255 66 369 8 27
Fragments arranged by size:
369 361 255 66 27 12 8
BsiHKAI G_wGCw'C
Cuts at: 0 741 1098
Size: 741 357
Bsp1286I G_dGCh'C
Cuts at: 0 27 122 177 628 698 741 781 854
Size: 27 95 55 451 70 43 40 73
Cuts at: 854 886 1098
Size: 32 212
Fragments arranged by size:
451 212 95 73 70 55 43 40
32 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 299 821 895 1098
Size: 251 48 522 74 203
Fragments arranged by size:
522 251 203 74 48
BsrBI CCG'CTC
Cuts at: 0 215 518 1098
Size: 215 303 580
Fragments arranged by size:
580 303 215
BsrFI r'CCGG_y
Cuts at: 0 256 369 572 1098
Size: 256 113 203 526
Fragments arranged by size:
526 256 203 113
BsrGI T'GTAC_A
Cuts at: 0 803 1098
Size: 803 295
BssSI C'ACGA_G
Cuts at: 0 511 1075 1098
Size: 511 564 23
Fragments arranged by size:
564 511 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 348 588 785 1098
Size: 318 30 240 197 313
Fragments arranged by size:
318 313 240 197 30
BstEII G'GTnAC_C
Cuts at: 0 432 1098
Size: 432 666
Bsu36I CC'TnA_GG
Cuts at: 0 724 1098
Size: 724 374
BtgI C'CryG_G
Cuts at: 0 204 219 1098
Size: 204 15 879
Fragments arranged by size:
879 204 15
EaeI y'GGCC_r
Cuts at: 0 254 922 1098
Size: 254 668 176
Fragments arranged by size:
668 254 176
EarI CTCTTCn'nnn_
Cuts at: 0 364 1098
Size: 364 734
EcoICRI GAG'CTC
Cuts at: 0 739 1098
Size: 739 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 724 779 868 1075 1098
Size: 477 247 55 89 207 23
Fragments arranged by size:
477 247 207 89 55 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 1098
Size: 67 1031
EcoO109I rG'GnC_Cy
Cuts at: 0 173 906 1010 1098
Size: 173 733 104 88
Fragments arranged by size:
733 173 104 88
HaeII r_GCGC'y
Cuts at: 0 422 644 679 1075 1098
Size: 422 222 35 396 23
Fragments arranged by size:
422 396 222 35 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 603 635 932 964 1098
Size: 451 32 120 32 297 32 134
Fragments arranged by size:
451 297 134 120 32 32 32
KasI G'GCGC_C
Cuts at: 0 640 675 1098
Size: 640 35 423
Fragments arranged by size:
640 423 35
MfeI C'AATT_G
Cuts at: 0 918 1098
Size: 918 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 494 1098
Size: 254 240 604
Fragments arranged by size:
604 254 240
MscI TGG'CCA
Cuts at: 0 924 1098
Size: 924 174
MslI CAynn'nnrTG
Cuts at: 0 1039 1098
Size: 1039 59
MspA1I CmG'CkG
Cuts at: 0 102 221 315 567 588 630 702 785
Size: 102 119 94 252 21 42 72 83
Cuts at: 785 1055 1098
Size: 270 43
Fragments arranged by size:
270 252 119 102 94 83 72 43
42 21
NaeI GCC'GGC
Cuts at: 0 258 574 1098
Size: 258 316 524
Fragments arranged by size:
524 316 258
NarI GG'CG_CC
Cuts at: 0 641 676 1098
Size: 641 35 422
Fragments arranged by size:
641 422 35
NgoMIV G'CCGG_C
Cuts at: 0 256 572 1098
Size: 256 316 526
Fragments arranged by size:
526 316 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 839 1098
Size: 651 188 259
Fragments arranged by size:
651 259 188
PfoI T'CCnGG_A
Cuts at: 0 362 1098
Size: 362 736
PmlI CAC'GTG
Cuts at: 0 168 1098
Size: 168 930
PspOMI G'GGCC_C
Cuts at: 0 173 624 1098
Size: 173 451 474
Fragments arranged by size:
474 451 173
PstI C_TGCA'G
Cuts at: 0 107 818 1098
Size: 107 711 280
Fragments arranged by size:
711 280 107
PvuII CAG'CTG
Cuts at: 0 102 567 702 1055 1098
Size: 102 465 135 353 43
Fragments arranged by size:
465 353 135 102 43
SacI G_AGCT'C
Cuts at: 0 741 1098
Size: 741 357
SacII CC_GC'GG
Cuts at: 0 222 1098
Size: 222 876
SapI GCTCTTCn'nnn_
Cuts at: 0 364 1098
Size: 364 734
SbfI CC_TGCA'GG
Cuts at: 0 818 1098
Size: 818 280
SfcI C'TryA_G
Cuts at: 0 103 595 814 1098
Size: 103 492 219 284
Fragments arranged by size:
492 284 219 103
SfoI GGC'GCC
Cuts at: 0 642 677 1098
Size: 642 35 421
Fragments arranged by size:
642 421 35
SmaI CCC'GGG
Cuts at: 0 54 430 857 1098
Size: 54 376 427 241
Fragments arranged by size:
427 376 241 54
StuI AGG'CCT
Cuts at: 0 529 1098
Size: 529 569
StyI C'CwwG_G
Cuts at: 0 377 1081 1098
Size: 377 704 17
Fragments arranged by size:
704 377 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 82 556 787 831 1098
Size: 21 61 474 231 44 267
Fragments arranged by size:
474 267 231 61 44 21
TatI w'GTAC_w
Cuts at: 0 803 1098
Size: 803 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 333 1014 1098
Size: 333 681 84
Fragments arranged by size:
681 333 84
XmaI C'CCGG_G
Cuts at: 0 52 428 855 1098
Size: 52 376 427 243
Fragments arranged by size:
427 376 243 52
XmnI GAAnn'nnTTC
Cuts at: 0 529 1098
Size: 529 569
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII
Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV PflMI PfoI PmlI PspOMI PstI
PvuII SacI SacII SapI SbfI SfcI SfoI SmaI
StuI StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BpuEI BsaI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BstBI
BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MluI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI ZraI