(Linear) (Six-base) MAPSORT of: KIEE0397.seq  Check: 2396  from: 1  to: 267



KIEE0397.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:32  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     147     267 
   Size:        147     120
 
AvaI C'yCGr_G

Cuts at:      0     221     267 
   Size:        221      46
 
BanI G'GyrC_C

Cuts at:      0     202     267 
   Size:        202      65
 
BanII G_rGCy'C

Cuts at:      0      41     267 
   Size:         41     226
 
BbeI G_GCGC'C

Cuts at:      0     206     267 
   Size:        206      61
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     267 
   Size:        173      94
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     233     267 
   Size:        233      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     267 
   Size:        121     146
 
BsaHI Gr'CG_yC

Cuts at:      0     203     257     267 
   Size:        203      54      10

  Fragments arranged by size: 

                 203      54      10
 
BseYI C'CCAG_C

Cuts at:      0      70     267 
   Size:         70     197
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     267 
   Size:         41     226
 
BtgI C'CryG_G

Cuts at:      0      41     267 
   Size:         41     226
 
EaeI y'GGCC_r

Cuts at:      0     138     267 
   Size:        138     129
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     267 
   Size:        178      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     267 
   Size:        121     146
 
HaeII r_GCGC'y

Cuts at:      0     206     267 
   Size:        206      61
 
KasI G'GCGC_C

Cuts at:      0     202     267 
   Size:        202      65
 
MscI TGG'CCA

Cuts at:      0     140     267 
   Size:        140     127
 
NarI GG'CG_CC

Cuts at:      0     203     267 
   Size:        203      64
 
NcoI C'CATG_G

Cuts at:      0      41     267 
   Size:         41     226
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147     267 
   Size:        147     120
 
PfoI T'CCnGG_A

Cuts at:      0     176     267 
   Size:        176      91
 
SfoI GGC'GCC

Cuts at:      0     204     267 
   Size:        204      63
 
SmaI CCC'GGG

Cuts at:      0     223     267 
   Size:        223      44
 
StuI AGG'CCT

Cuts at:      0     124     267 
   Size:        124     143
 
StyI C'CwwG_G

Cuts at:      0      41     135     267 
   Size:         41      94     132

  Fragments arranged by size: 

                 132      94      41
 
XmaI C'CCGG_G

Cuts at:      0     221     267 
   Size:        221      46
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BpmI
    BsaHI    BseYI Bsp1286I     BtgI     EaeI     EarI  Eco57MI    HaeII
     KasI     MscI     NarI     NcoI    PflMI     PfoI     SfoI     SmaI
     StuI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI