Restriction map for
pFN21AE0359
(Linear) (Six-base) MAPSORT of: KIEE0359.seq Check: 253 from: 1 to: 1746
KIEE0359.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
Acc65I G'GTAC_C
Cuts at: 0 107 1746
Size: 107 1639
AflIII A'CryG_T
Cuts at: 0 533 1746
Size: 533 1213
AlwNI CAG_nnn'CTG
Cuts at: 0 640 1552 1746
Size: 640 912 194
Fragments arranged by size:
912 640 194
ApoI r'AATT_y
Cuts at: 0 505 1389 1496 1540 1746
Size: 505 884 107 44 206
Fragments arranged by size:
884 505 206 107 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 763 796 1746
Size: 763 33 950
Fragments arranged by size:
950 763 33
BanI G'GyrC_C
Cuts at: 0 59 91 107 123 1746
Size: 59 32 16 16 1623
Fragments arranged by size:
1623 59 32 16 16
BanII G_rGCy'C
Cuts at: 0 256 281 1746
Size: 256 25 1465
Fragments arranged by size:
1465 256 25
BbeI G_GCGC'C
Cuts at: 0 127 1746
Size: 127 1619
BbsI GAAGACnn'nnnn_
Cuts at: 0 1463 1746
Size: 1463 283
BbvCI CC'TCA_GC
Cuts at: 0 187 1746
Size: 187 1559
BclI T'GATC_A
Cuts at: 0 1590 1746
Size: 1590 156
BglI GCCn_nnn'nGGC
Cuts at: 0 121 218 1746
Size: 121 97 1528
Fragments arranged by size:
1528 121 97
BlpI GC'TnA_GC
Cuts at: 0 1735 1746
Size: 1735 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 1105 1746
Size: 1105 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 713 1334 1746
Size: 662 51 621 412
Fragments arranged by size:
662 621 412 51
Bpu10I CC'TnA_GC
Cuts at: 0 187 1746
Size: 187 1559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1746
Size: 936 810
BsaBI GATnn'nnATC
Cuts at: 0 815 1746
Size: 815 931
BsaHI Gr'CG_yC
Cuts at: 0 124 1746
Size: 124 1622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 122 127 261 264 1196 1746
Size: 37 85 5 134 3 932 550
Fragments arranged by size:
932 550 134 85 37 5 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1746
Size: 1091 655
BsiEI CG_ry'CG
Cuts at: 0 208 1746
Size: 208 1538
Bsp1286I G_dGCh'C
Cuts at: 0 256 281 1746
Size: 256 25 1465
Fragments arranged by size:
1465 256 25
BspHI T'CATG_A
Cuts at: 0 1452 1746
Size: 1452 294
BsrFI r'CCGG_y
Cuts at: 0 144 1746
Size: 144 1602
BstXI CCAn_nnnn'nTGG
Cuts at: 0 886 1746
Size: 886 860
BstYI r'GATC_y
Cuts at: 0 1482 1746
Size: 1482 264
Bsu36I CC'TnA_GG
Cuts at: 0 25 1746
Size: 25 1721
BtgI C'CryG_G
Cuts at: 0 160 213 281 1746
Size: 160 53 68 1465
Fragments arranged by size:
1465 160 68 53
BtsI GCAGTG_nn'
Cuts at: 0 461 1746
Size: 461 1285
DraIII CAC_nnn'GTG
Cuts at: 0 1417 1746
Size: 1417 329
EaeI y'GGCC_r
Cuts at: 0 205 269 1746
Size: 205 64 1477
Fragments arranged by size:
1477 205 64
EagI C'GGCC_G
Cuts at: 0 205 1746
Size: 205 1541
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 713 1334 1746
Size: 662 51 621 412
Fragments arranged by size:
662 621 412 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 905 1746
Size: 905 841
EcoO109I rG'GnC_Cy
Cuts at: 0 449 1746
Size: 449 1297
EcoRI G'AATT_C
Cuts at: 0 1540 1746
Size: 1540 206
FspI TGC'GCA
Cuts at: 0 1396 1746
Size: 1396 350
HaeII r_GCGC'y
Cuts at: 0 127 1746
Size: 127 1619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 468 500 520 552 973 1005 1066 1098
Size: 468 32 20 32 421 32 61 32
Cuts at: 1098 1746
Size: 648
Fragments arranged by size:
648 468 421 61 32 32 32 32
20
HindIII A'AGCT_T
Cuts at: 0 839 1746
Size: 839 907
KasI G'GCGC_C
Cuts at: 0 123 1746
Size: 123 1623
KpnI G_GTAC'C
Cuts at: 0 111 1746
Size: 111 1635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 1198 1746
Size: 552 646 548
Fragments arranged by size:
646 552 548
MslI CAynn'nnrTG
Cuts at: 0 491 821 824 884 1746
Size: 491 330 3 60 862
Fragments arranged by size:
862 491 330 60 3
MspA1I CmG'CkG
Cuts at: 0 65 162 215 1746
Size: 65 97 53 1531
Fragments arranged by size:
1531 97 65 53
NarI GG'CG_CC
Cuts at: 0 124 1746
Size: 124 1622
NcoI C'CATG_G
Cuts at: 0 281 1746
Size: 281 1465
NdeI CA'TA_TG
Cuts at: 0 993 1224 1746
Size: 993 231 522
Fragments arranged by size:
993 522 231
NruI TCG'CGA
Cuts at: 0 52 1746
Size: 52 1694
NspI r_CATG'y
Cuts at: 0 537 1401 1746
Size: 537 864 345
Fragments arranged by size:
864 537 345
PciI A'CATG_T
Cuts at: 0 533 1746
Size: 533 1213
PstI C_TGCA'G
Cuts at: 0 1236 1746
Size: 1236 510
SacII CC_GC'GG
Cuts at: 0 163 216 1746
Size: 163 53 1530
Fragments arranged by size:
1530 163 53
SexAI A'CCwGG_T
Cuts at: 0 1413 1746
Size: 1413 333
SfcI C'TryA_G
Cuts at: 0 1232 1746
Size: 1232 514
SfoI GGC'GCC
Cuts at: 0 125 1746
Size: 125 1621
SmlI C'TyrA_G
Cuts at: 0 915 1746
Size: 915 831
StyI C'CwwG_G
Cuts at: 0 281 1682 1746
Size: 281 1401 64
Fragments arranged by size:
1401 281 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1403 1435 1515 1547 1746
Size: 1403 32 80 32 199
Fragments arranged by size:
1403 199 80 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 362 1746
Size: 362 1384
XmnI GAAnn'nnTTC
Cuts at: 0 896 1746
Size: 896 850
Enzymes that do cut:
Acc65I AflIII AlwNI ApoI BaeI BanI BanII BbeI
BbsI BbvCI BclI BglI BlpI BmrI BpmI Bpu10I
BpuEI BsaBI BsaHI BseRI BsgI BsiEI Bsp1286I BspHI
BsrFI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI
NdeI NruI NspI PciI PstI SacII SexAI SfcI
SfoI SmlI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BcgI BciVI BfrBI BglII
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaWI BsaXI
BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EarI EciI EcoICRI
EcoRV FalI FseI FspAI HincII HpaI MfeI MluI
MscI NaeI NgoMIV NheI NotI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI XbaI XcmI XhoI XmaI ZraI