Restriction map for
pFN21AE0300
(Linear) (Six-base) MAPSORT of: KIEE0300.seq Check: 652 from: 1 to: 4509
KIEE0300.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 123 3558 3995 4193 4509
Size: 123 3435 437 198 316
Fragments arranged by size:
3435 437 316 198 123
AccI GT'mk_AC
Cuts at: 0 1259 4091 4509
Size: 1259 2832 418
Fragments arranged by size:
2832 1259 418
Acc65I G'GTAC_C
Cuts at: 0 2442 2484 4509
Size: 2442 42 2025
Fragments arranged by size:
2442 2025 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 577 685 1220 1306 2218 2285 2296
Size: 559 18 108 535 86 912 67 11
Cuts at: 2296 4509
Size: 2213
Fragments arranged by size:
2213 912 559 535 108 86 67 18
11
AfeI AGC'GCT
Cuts at: 0 1709 3023 4509
Size: 1709 1314 1486
Fragments arranged by size:
1709 1486 1314
AflII C'TTAA_G
Cuts at: 0 2245 4509
Size: 2245 2264
AflIII A'CryG_T
Cuts at: 0 1866 2527 4509
Size: 1866 661 1982
Fragments arranged by size:
1982 1866 661
AhdI GACnn_n'nnGTC
Cuts at: 0 2946 4509
Size: 2946 1563
AleI CACnn'nnGTG
Cuts at: 0 3256 4509
Size: 3256 1253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 2434 3452 4509
Size: 113 2321 1018 1057
Fragments arranged by size:
2321 1057 1018 113
AlwNI CAG_nnn'CTG
Cuts at: 0 1413 1549 2584 2968 3602 4509
Size: 1413 136 1035 384 634 907
Fragments arranged by size:
1413 1035 907 634 384 136
ApaI G_GGCC'C
Cuts at: 0 1556 3760 4133 4509
Size: 1556 2204 373 376
Fragments arranged by size:
2204 1556 376 373
ApaLI G'TGCA_C
Cuts at: 0 1207 4509
Size: 1207 3302
ApoI r'AATT_y
Cuts at: 0 232 2603 4509
Size: 232 2371 1906
Fragments arranged by size:
2371 1906 232
AseI AT'TA_AT
Cuts at: 0 172 4509
Size: 172 4337
AvaI C'yCGr_G
Cuts at: 0 1882 3118 3142 3337 3360 3409 3424 3805
Size: 1882 1236 24 195 23 49 15 381
Cuts at: 3805 4036 4509
Size: 231 473
Fragments arranged by size:
1882 1236 473 381 231 195 49 24
23 15
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2369 2402 4509
Size: 2369 33 2107
Fragments arranged by size:
2369 2107 33
BanI G'GyrC_C
Cuts at: 0 108 2442 2484 3148 3983 4395 4509
Size: 108 2334 42 664 835 412 114
Fragments arranged by size:
2334 835 664 412 114 108 42
BanII G_rGCy'C
Cuts at: 0 1556 1891 3338 3760 4133 4509
Size: 1556 335 1447 422 373 376
Fragments arranged by size:
1556 1447 422 376 373 335
BbeI G_GCGC'C
Cuts at: 0 112 4399 4509
Size: 112 4287 110
Fragments arranged by size:
4287 112 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 306 2417 2801 3590 4499 4509
Size: 287 19 2111 384 789 909 10
Fragments arranged by size:
2111 909 789 384 287 19 10
BbvCI CC'TCA_GC
Cuts at: 0 3916 3964 4509
Size: 3916 48 545
Fragments arranged by size:
3916 545 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1330 1364 3688 3722 4509
Size: 1330 34 2324 34 787
Fragments arranged by size:
2324 1330 787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 4509
Size: 207 4302
BlpI GC'TnA_GC
Cuts at: 0 1442 4309 4509
Size: 1442 2867 200
Fragments arranged by size:
2867 1442 200
Bme1580I G_kGCm'C
Cuts at: 0 478 1211 1556 3068 3151 3760 3956 4133
Size: 478 733 345 1512 83 609 196 177
Cuts at: 4133 4509
Size: 376
Fragments arranged by size:
1512 733 609 478 376 345 196 177
83
BmrI ACTGGGnnnn_n'
Cuts at: 0 1931 2770 4509
Size: 1931 839 1739
Fragments arranged by size:
1931 1739 839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1144 1622 2956 3019 3495 3624 4509
Size: 914 230 478 1334 63 476 129 885
Fragments arranged by size:
1334 914 885 478 476 230 129 63
Bpu10I CC'TnA_GC
Cuts at: 0 1414 1774 3916 3964 4162 4198 4509
Size: 1414 360 2142 48 198 36 311
Fragments arranged by size:
2142 1414 360 311 198 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1810 4509
Size: 374 1436 2699
Fragments arranged by size:
2699 1436 374
BsaI GGTCTCn'nnnn_
Cuts at: 0 1004 1182 2668 3931 4102 4509
Size: 1004 178 1486 1263 171 407
Fragments arranged by size:
1486 1263 1004 407 178 171
BsaAI yAC'GTr
Cuts at: 0 1869 2528 4509
Size: 1869 659 1981
Fragments arranged by size:
1981 1869 659
BsaHI Gr'CG_yC
Cuts at: 0 109 4396 4509
Size: 109 4287 113
Fragments arranged by size:
4287 113 109
BsaWI w'CCGG_w
Cuts at: 0 1515 4509
Size: 1515 2994
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1302 1807 2686 2895 3367 3871 4154 4509
Size: 1302 505 879 209 472 504 283 355
Fragments arranged by size:
1302 879 505 504 472 355 283 209
BseYI C'CCAG_C
Cuts at: 0 70 303 345 1118 1362 1760 2519 2961
Size: 70 233 42 773 244 398 759 442
Cuts at: 2961 3563 4125 4170 4176 4509
Size: 602 562 45 6 333
Fragments arranged by size:
773 759 602 562 442 398 333 244
233 70 45 42 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 177 760 769 2458 3651 4509
Size: 119 58 583 9 1689 1193 858
Fragments arranged by size:
1689 1193 858 583 119 58 9
BsiEI CG_ry'CG
Cuts at: 0 3660 4509
Size: 3660 849
BsiHKAI G_wGCw'C
Cuts at: 0 695 1211 2413 3947 4372 4509
Size: 695 516 1202 1534 425 137
Fragments arranged by size:
1534 1202 695 516 425 137
BsmI GAATG_Cn'
Cuts at: 0 1327 2256 3621 4509
Size: 1327 929 1365 888
Fragments arranged by size:
1365 1327 929 888
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3262 4509
Size: 3262 1247
Bsp1286I G_dGCh'C
Cuts at: 0 478 695 1211 1556 1891 2413 3068 3151
Size: 478 217 516 345 335 522 655 83
Cuts at: 3151 3338 3760 3947 3956 4133 4372 4509
Size: 187 422 187 9 177 239 137
Fragments arranged by size:
655 522 516 478 422 345 335 239
217 187 187 177 137 83 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 857 1235 2004 2430 3558 3899 3914
Size: 123 734 378 769 426 1128 341 15
Cuts at: 3914 3995 4193 4313 4509
Size: 81 198 120 196
Fragments arranged by size:
1128 769 734 426 378 341 198 196
123 120 81 15
BsrBI CCG'CTC
Cuts at: 0 3139 4166 4509
Size: 3139 1027 343
Fragments arranged by size:
3139 1027 343
BsrDI GCAATG_nn'
Cuts at: 0 2416 4430 4509
Size: 2416 2014 79
Fragments arranged by size:
2416 2014 79
BsrFI r'CCGG_y
Cuts at: 0 12 61 1358 2813 3078 3232 4359 4509
Size: 12 49 1297 1455 265 154 1127 150
Fragments arranged by size:
1455 1297 1127 265 154 150 49 12
BssSI C'ACGA_G
Cuts at: 0 1500 4509
Size: 1500 3009
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 783 1215 3951 4509
Size: 774 9 432 2736 558
Fragments arranged by size:
2736 774 558 432 9
BstBI TT'CG_AA
Cuts at: 0 2459 4509
Size: 2459 2050
BstEII G'GTnAC_C
Cuts at: 0 658 4509
Size: 658 3851
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3519 4125 4509
Size: 3519 606 384
Fragments arranged by size:
3519 606 384
BstYI r'GATC_y
Cuts at: 0 1545 3040 4275 4509
Size: 1545 1495 1235 234
Fragments arranged by size:
1545 1495 1235 234
Bsu36I CC'TnA_GG
Cuts at: 0 1591 4182 4509
Size: 1591 2591 327
Fragments arranged by size:
2591 1591 327
BtgI C'CryG_G
Cuts at: 0 749 1193 3173 3578 3680 4509
Size: 749 444 1980 405 102 829
Fragments arranged by size:
1980 829 749 444 405 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 933 2794 3194 3251 3650 4509
Size: 933 1861 400 57 399 859
Fragments arranged by size:
1861 933 859 400 399 57
BtsI GCAGTG_nn'
Cuts at: 0 72 1279 4030 4509
Size: 72 1207 2751 479
Fragments arranged by size:
2751 1207 479 72
DraI TTT'AAA
Cuts at: 0 1992 2849 4509
Size: 1992 857 1660
Fragments arranged by size:
1992 1660 857
DraIII CAC_nnn'GTG
Cuts at: 0 3951 4509
Size: 3951 558
DrdI GACnn_nn'nnGTC
Cuts at: 0 1255 4509
Size: 1255 3254
EaeI y'GGCC_r
Cuts at: 0 758 1575 3186 4013 4384 4509
Size: 758 817 1611 827 371 125
Fragments arranged by size:
1611 827 817 758 371 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 3366 4340 4496 4509
Size: 39 3327 974 156 13
Fragments arranged by size:
3327 974 156 39 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 577 685 914 1144 1220 1306 1622
Size: 559 18 108 229 230 76 86 316
Cuts at: 1622 2218 2285 2296 2956 3019 3495 3624 4509
Size: 596 67 11 660 63 476 129 885
Fragments arranged by size:
885 660 596 559 476 316 230 229
129 108 86 76 67 63 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2935 3832 4509
Size: 2935 897 677
Fragments arranged by size:
2935 897 677
EcoO109I rG'GnC_Cy
Cuts at: 0 210 1410 1596 2645 3357 3756 3888 4129
Size: 210 1200 186 1049 712 399 132 241
Cuts at: 4129 4130 4509
Size: 1 379
Fragments arranged by size:
1200 1049 712 399 379 241 210 186
132 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 795 4509
Size: 471 324 3714
Fragments arranged by size:
3714 471 324
FspI TGC'GCA
Cuts at: 0 679 4509
Size: 679 3830
HaeII r_GCGC'y
Cuts at: 0 112 1711 1774 3025 4399 4509
Size: 112 1599 63 1251 1374 110
Fragments arranged by size:
1599 1374 1251 112 110 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 2629 2661 2835 2867 2932 2964
Size: 274 32 2323 32 174 32 65 32
Cuts at: 2964 3117 3149 3313 3331 3345 3363 4252 4284
Size: 153 32 164 18 14 18 889 32
Cuts at: 4284 4509
Size: 225
Fragments arranged by size:
2323 889 274 225 174 164 153 65
32 32 32 32 32 32 18 18
14
HincII GTy'rAC
Cuts at: 0 1260 4509
Size: 1260 3249
KasI G'GCGC_C
Cuts at: 0 108 4395 4509
Size: 108 4287 114
Fragments arranged by size:
4287 114 108
KpnI G_GTAC'C
Cuts at: 0 2446 2488 4509
Size: 2446 42 2021
Fragments arranged by size:
2446 2021 42
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 848 1085 1340 1620 3908 4509
Size: 582 266 237 255 280 2288 601
Fragments arranged by size:
2288 601 582 280 266 255 237
MslI CAynn'nnrTG
Cuts at: 0 2225 3256 4509
Size: 2225 1031 1253
Fragments arranged by size:
2225 1253 1031
MspA1I CmG'CkG
Cuts at: 0 751 1122 1195 1245 1574 3194 3280 3632
Size: 751 371 73 50 329 1620 86 352
Cuts at: 3632 4012 4509
Size: 380 497
Fragments arranged by size:
1620 751 497 380 371 352 329 86
73 50
NaeI GCC'GGC
Cuts at: 0 14 63 2815 3080 3234 4509
Size: 14 49 2752 265 154 1275
Fragments arranged by size:
2752 1275 265 154 49 14
NarI GG'CG_CC
Cuts at: 0 109 4396 4509
Size: 109 4287 113
Fragments arranged by size:
4287 113 109
NgoMIV G'CCGG_C
Cuts at: 0 12 61 2813 3078 3232 4509
Size: 12 49 2752 265 154 1277
Fragments arranged by size:
2752 1277 265 154 49 12
NspI r_CATG'y
Cuts at: 0 699 2340 4509
Size: 699 1641 2169
Fragments arranged by size:
2169 1641 699
PasI CC'CwG_GG
Cuts at: 0 3288 4509
Size: 3288 1221
PflMI CCAn_nnn'nTGG
Cuts at: 0 2634 4509
Size: 2634 1875
PfoI T'CCnGG_A
Cuts at: 0 2546 2753 2933 4509
Size: 2546 207 180 1576
Fragments arranged by size:
2546 1576 207 180
PmlI CAC'GTG
Cuts at: 0 1869 2528 4509
Size: 1869 659 1981
Fragments arranged by size:
1981 1869 659
PpuMI rG'GwC_Cy
Cuts at: 0 210 1596 2645 3357 3888 4509
Size: 210 1386 1049 712 531 621
Fragments arranged by size:
1386 1049 712 621 531 210
PshAI GACnn'nnGTC
Cuts at: 0 2573 4509
Size: 2573 1936
PspOMI G'GGCC_C
Cuts at: 0 1552 3756 4129 4509
Size: 1552 2204 373 380
Fragments arranged by size:
2204 1552 380 373
PstI C_TGCA'G
Cuts at: 0 123 728 972 1238 4509
Size: 123 605 244 266 3271
Fragments arranged by size:
3271 605 266 244 123
PvuI CG_AT'CG
Cuts at: 0 3660 4509
Size: 3660 849
PvuII CAG'CTG
Cuts at: 0 1122 3280 4509
Size: 1122 2158 1229
Fragments arranged by size:
2158 1229 1122
SacII CC_GC'GG
Cuts at: 0 752 1196 4509
Size: 752 444 3313
Fragments arranged by size:
3313 752 444
SalI G'TCGA_C
Cuts at: 0 1258 4509
Size: 1258 3251
SanDI GG'GwC_CC
Cuts at: 0 1596 4509
Size: 1596 2913
ScaI AGT'ACT
Cuts at: 0 4342 4509
Size: 4342 167
SfcI C'TryA_G
Cuts at: 0 119 371 724 968 1234 4509
Size: 119 252 353 244 266 3275
Fragments arranged by size:
3275 353 266 252 244 119
SfoI GGC'GCC
Cuts at: 0 110 4397 4509
Size: 110 4287 112
Fragments arranged by size:
4287 112 110
SgrAI Cr'CCGG_yG
Cuts at: 0 12 4509
Size: 12 4497
SmaI CCC'GGG
Cuts at: 0 3362 4509
Size: 3362 1147
SmlI C'TyrA_G
Cuts at: 0 389 1789 2245 4509
Size: 389 1400 456 2264
Fragments arranged by size:
2264 1400 456 389
SpeI A'CTAG_T
Cuts at: 0 3060 4509
Size: 3060 1449
SspI AAT'ATT
Cuts at: 0 170 336 588 1952 4509
Size: 170 166 252 1364 2557
Fragments arranged by size:
2557 1364 252 170 166
StyI C'CwwG_G
Cuts at: 0 3380 4509
Size: 3380 1129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 994 1411 3955 4474 4509
Size: 646 348 417 2544 519 35
Fragments arranged by size:
2544 646 519 417 348 35
TatI w'GTAC_w
Cuts at: 0 1801 1828 4340 4509
Size: 1801 27 2512 169
Fragments arranged by size:
2512 1801 169 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1359 2656 3723 4509
Size: 1359 1297 1067 786
Fragments arranged by size:
1359 1297 1067 786
XmaI C'CCGG_G
Cuts at: 0 3360 4509
Size: 3360 1149
XmnI GAAnn'nnTTC
Cuts at: 0 620 4509
Size: 620 3889
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII KasI KpnI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuI PvuII
SacII SalI SanDI ScaI SfcI SfoI SgrAI SmaI
SmlI SpeI SspI StyI TaqII TatI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AgeI AloI AscI AsiSI AvrII BamHI
BclI BfrBI BglI BglII BmgBI BmtI BplI BsaBI
BsaXI BsiWI BspEI BspHI BsrGI BssHII BstZ17I ClaI
CspCI EagI EarI EcoICRI EcoRI EcoRV FseI FspAI
HindIII HpaI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PspXI PsrI RsrII SacI SapI SbfI SexAI SfiI
SgfI SnaBI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI ZraI