Restriction map for
pFN21AE0296
(Linear) (Six-base) MAPSORT of: KIEE0296.seq Check: 9252 from: 1 to: 963
KIEE0296.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
Acc65I G'GTAC_C
Cuts at: 0 640 963
Size: 640 323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 841 963
Size: 510 331 122
Fragments arranged by size:
510 331 122
AhdI GACnn_n'nnGTC
Cuts at: 0 729 891 963
Size: 729 162 72
Fragments arranged by size:
729 162 72
AlwNI CAG_nnn'CTG
Cuts at: 0 69 336 963
Size: 69 267 627
Fragments arranged by size:
627 267 69
ApaI G_GGCC'C
Cuts at: 0 275 434 963
Size: 275 159 529
Fragments arranged by size:
529 275 159
AscI GG'CGCG_CC
Cuts at: 0 143 963
Size: 143 820
AvaI C'yCGr_G
Cuts at: 0 148 433 754 963
Size: 148 285 321 209
Fragments arranged by size:
321 285 209 148
AvrII C'CTAG_G
Cuts at: 0 801 963
Size: 801 162
BanI G'GyrC_C
Cuts at: 0 245 277 343 589 640 805 963
Size: 245 32 66 246 51 165 158
Fragments arranged by size:
246 245 165 158 66 51 32
BanII G_rGCy'C
Cuts at: 0 174 275 434 488 963
Size: 174 101 159 54 475
Fragments arranged by size:
475 174 159 101 54
BbeI G_GCGC'C
Cuts at: 0 249 281 347 809 963
Size: 249 32 66 462 154
Fragments arranged by size:
462 249 154 66 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 829 963
Size: 285 544 134
Fragments arranged by size:
544 285 134
BglI GCCn_nnn'nGGC
Cuts at: 0 58 160 341 963
Size: 58 102 181 622
Fragments arranged by size:
622 181 102 58
BlpI GC'TnA_GC
Cuts at: 0 46 963
Size: 46 917
Bme1580I G_kGCm'C
Cuts at: 0 63 275 434 592 963
Size: 63 212 159 158 371
Fragments arranged by size:
371 212 159 158 63
BmgBI CAC'GTC
Cuts at: 0 81 492 963
Size: 81 411 471
Fragments arranged by size:
471 411 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 418 502 963
Size: 307 111 84 461
Fragments arranged by size:
461 307 111 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 963
Size: 637 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 770 963
Size: 121 649 193
Fragments arranged by size:
649 193 121
BsaAI yAC'GTr
Cuts at: 0 366 963
Size: 366 597
BsaHI Gr'CG_yC
Cuts at: 0 29 246 278 344 410 806 963
Size: 29 217 32 66 66 396 157
Fragments arranged by size:
396 217 157 66 66 32 29
BsaWI w'CCGG_w
Cuts at: 0 841 963
Size: 841 122
BseYI C'CCAG_C
Cuts at: 0 369 378 677 706 963
Size: 369 9 299 29 257
Fragments arranged by size:
369 299 257 29 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 454 963
Size: 294 160 509
Fragments arranged by size:
509 294 160
BsiEI CG_ry'CG
Cuts at: 0 627 963
Size: 627 336
Bsp1286I G_dGCh'C
Cuts at: 0 63 174 275 434 488 592 963
Size: 63 111 101 159 54 104 371
Fragments arranged by size:
371 159 111 104 101 63 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 332 569 963
Size: 332 237 394
Fragments arranged by size:
394 332 237
BsrBI CCG'CTC
Cuts at: 0 57 963
Size: 57 906
BsrFI r'CCGG_y
Cuts at: 0 16 340 605 963
Size: 16 324 265 358
Fragments arranged by size:
358 324 265 16
BssHII G'CGCG_C
Cuts at: 0 143 495 963
Size: 143 352 468
Fragments arranged by size:
468 352 143
BssSI C'ACGA_G
Cuts at: 0 583 963
Size: 583 380
BtgI C'CryG_G
Cuts at: 0 174 266 389 404 662 963
Size: 174 92 123 15 258 301
Fragments arranged by size:
301 258 174 123 92 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 618 963
Size: 618 345
BtsI GCAGTG_nn'
Cuts at: 0 439 963
Size: 439 524
EaeI y'GGCC_r
Cuts at: 0 74 338 386 624 671 963
Size: 74 264 48 238 47 292
Fragments arranged by size:
292 264 238 74 48 47
EagI C'GGCC_G
Cuts at: 0 624 963
Size: 624 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 963
Size: 25 938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 418 502 510 841 963
Size: 307 111 84 8 331 122
Fragments arranged by size:
331 307 122 111 84 8
EcoO109I rG'GnC_Cy
Cuts at: 0 153 271 430 454 549 963
Size: 153 118 159 24 95 414
Fragments arranged by size:
414 159 153 118 95 24
HaeII r_GCGC'y
Cuts at: 0 53 70 166 186 249 281 336 347
Size: 53 17 96 20 63 32 55 11
Cuts at: 347 684 809 963
Size: 337 125 154
Fragments arranged by size:
337 154 125 96 63 55 53 32
20 17 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 877 909 963
Size: 715 32 130 32 54
Fragments arranged by size:
715 130 54 32 32
HincII GTy'rAC
Cuts at: 0 927 963
Size: 927 36
KasI G'GCGC_C
Cuts at: 0 245 277 343 805 963
Size: 245 32 66 462 158
Fragments arranged by size:
462 245 158 66 32
KpnI G_GTAC'C
Cuts at: 0 644 963
Size: 644 319
MslI CAynn'nnrTG
Cuts at: 0 595 766 963
Size: 595 171 197
Fragments arranged by size:
595 197 171
MspA1I CmG'CkG
Cuts at: 0 35 176 268 391 480 963
Size: 35 141 92 123 89 483
Fragments arranged by size:
483 141 123 92 89 35
NaeI GCC'GGC
Cuts at: 0 342 963
Size: 342 621
NarI GG'CG_CC
Cuts at: 0 246 278 344 806 963
Size: 246 32 66 462 157
Fragments arranged by size:
462 246 157 66 32
NgoMIV G'CCGG_C
Cuts at: 0 340 963
Size: 340 623
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 963
Size: 336 627
PpuMI rG'GwC_Cy
Cuts at: 0 454 963
Size: 454 509
PspOMI G'GGCC_C
Cuts at: 0 271 430 963
Size: 271 159 533
Fragments arranged by size:
533 271 159
PstI C_TGCA'G
Cuts at: 0 446 566 963
Size: 446 120 397
Fragments arranged by size:
446 397 120
SacII CC_GC'GG
Cuts at: 0 177 269 392 963
Size: 177 92 123 571
Fragments arranged by size:
571 177 123 92
SanDI GG'GwC_CC
Cuts at: 0 454 963
Size: 454 509
SfcI C'TryA_G
Cuts at: 0 442 562 963
Size: 442 120 401
Fragments arranged by size:
442 401 120
SfoI GGC'GCC
Cuts at: 0 247 279 345 807 963
Size: 247 32 66 462 156
Fragments arranged by size:
462 247 156 66 32
SmlI C'TyrA_G
Cuts at: 0 616 963
Size: 616 347
StuI AGG'CCT
Cuts at: 0 361 963
Size: 361 602
StyI C'CwwG_G
Cuts at: 0 356 545 801 963
Size: 356 189 256 162
Fragments arranged by size:
356 256 189 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 963
Size: 423 540
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApaI AscI AvaI AvrII
BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseYI BsgI
BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BtgI
BtgZI BtsI EaeI EagI EciI Eco57MI EcoO109I HaeII
Hin4I HincII KasI KpnI MslI MspA1I NaeI NarI
NgoMIV PflMI PpuMI PspOMI PstI SacII SanDI SfcI
SfoI SmlI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI Bpu10I BsaBI BsaXI BseRI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI MmeI
MscI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI