Restriction map for
pFN21AE0290
(Linear) (Six-base) MAPSORT of: KIEE0290.seq Check: 7964 from: 1 to: 876
KIEE0290.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 624 876
Size: 624 252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 640 876
Size: 102 538 236
Fragments arranged by size:
538 236 102
AfeI AGC'GCT
Cuts at: 0 439 876
Size: 439 437
AflIII A'CryG_T
Cuts at: 0 740 742 876
Size: 740 2 134
Fragments arranged by size:
740 134 2
AlwNI CAG_nnn'CTG
Cuts at: 0 125 876
Size: 125 751
ApaI G_GGCC'C
Cuts at: 0 100 412 558 703 876
Size: 100 312 146 145 173
Fragments arranged by size:
312 173 146 145 100
AvaI C'yCGr_G
Cuts at: 0 93 353 625 876
Size: 93 260 272 251
Fragments arranged by size:
272 260 251 93
BanI G'GyrC_C
Cuts at: 0 18 757 876
Size: 18 739 119
Fragments arranged by size:
739 119 18
BanII G_rGCy'C
Cuts at: 0 100 146 412 457 558 578 703 876
Size: 100 46 266 45 101 20 125 173
Fragments arranged by size:
266 173 125 101 100 46 45 20
BbeI G_GCGC'C
Cuts at: 0 22 761 876
Size: 22 739 115
Fragments arranged by size:
739 115 22
BglI GCCn_nnn'nGGC
Cuts at: 0 598 860 876
Size: 598 262 16
Fragments arranged by size:
598 262 16
BlpI GC'TnA_GC
Cuts at: 0 451 876
Size: 451 425
Bme1580I G_kGCm'C
Cuts at: 0 71 100 412 558 703 876
Size: 71 29 312 146 145 173
Fragments arranged by size:
312 173 146 145 71 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 451 876
Size: 415 36 425
Fragments arranged by size:
425 415 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 876
Size: 679 197
BsaAI yAC'GTr
Cuts at: 0 84 743 876
Size: 84 659 133
Fragments arranged by size:
659 133 84
BsaHI Gr'CG_yC
Cuts at: 0 19 538 758 876
Size: 19 519 220 118
Fragments arranged by size:
519 220 118 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 876
Size: 577 30 269
Fragments arranged by size:
577 269 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 222 225 228 448 562 876
Size: 222 3 3 220 114 314
Fragments arranged by size:
314 222 220 114 3 3
BseYI C'CCAG_C
Cuts at: 0 70 285 297 486 876
Size: 70 215 12 189 390
Fragments arranged by size:
390 215 189 70 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 876
Size: 568 308
BsiEI CG_ry'CG
Cuts at: 0 42 572 608 876
Size: 42 530 36 268
Fragments arranged by size:
530 268 42 36
Bsp1286I G_dGCh'C
Cuts at: 0 71 100 146 412 457 558 578 703
Size: 71 29 46 266 45 101 20 125
Cuts at: 703 876
Size: 173
Fragments arranged by size:
266 173 125 101 71 46 45 29
20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 624 876
Size: 624 252
BsrBI CCG'CTC
Cuts at: 0 26 552 798 876
Size: 26 526 246 78
Fragments arranged by size:
526 246 78 26
BsrFI r'CCGG_y
Cuts at: 0 291 876
Size: 291 585
BssHII G'CGCG_C
Cuts at: 0 374 387 389 494 876
Size: 374 13 2 105 382
Fragments arranged by size:
382 374 105 13 2
BssSI C'ACGA_G
Cuts at: 0 64 876
Size: 64 812
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 876
Size: 168 708
BtgI C'CryG_G
Cuts at: 0 36 56 198 876
Size: 36 20 142 678
Fragments arranged by size:
678 142 36 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 682 876
Size: 682 194
EaeI y'GGCC_r
Cuts at: 0 39 569 605 876
Size: 39 530 36 271
Fragments arranged by size:
530 271 39 36
EagI C'GGCC_G
Cuts at: 0 39 569 605 876
Size: 39 530 36 271
Fragments arranged by size:
530 271 39 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 415 451 640 876
Size: 102 313 36 189 236
Fragments arranged by size:
313 236 189 102 36
EcoO109I rG'GnC_Cy
Cuts at: 0 350 409 555 699 700 876
Size: 350 59 146 144 1 176
Fragments arranged by size:
350 176 146 144 59 1
HaeII r_GCGC'y
Cuts at: 0 22 64 77 92 286 298 441 676
Size: 22 42 13 15 194 12 143 235
Cuts at: 676 761 845 876
Size: 85 84 31
Fragments arranged by size:
235 194 143 85 84 42 31 22
15 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 876
Size: 334 32 510
Fragments arranged by size:
510 334 32
KasI G'GCGC_C
Cuts at: 0 18 757 876
Size: 18 739 119
Fragments arranged by size:
739 119 18
MspA1I CmG'CkG
Cuts at: 0 38 200 764 876
Size: 38 162 564 112
Fragments arranged by size:
564 162 112 38
NaeI GCC'GGC
Cuts at: 0 293 876
Size: 293 583
NarI GG'CG_CC
Cuts at: 0 19 758 876
Size: 19 739 118
Fragments arranged by size:
739 118 19
NcoI C'CATG_G
Cuts at: 0 56 876
Size: 56 820
NgoMIV G'CCGG_C
Cuts at: 0 291 876
Size: 291 585
NotI GC'GGCC_GC
Cuts at: 0 39 605 876
Size: 39 566 271
Fragments arranged by size:
566 271 39
PasI CC'CwG_GG
Cuts at: 0 14 876
Size: 14 862
PfoI T'CCnGG_A
Cuts at: 0 124 876
Size: 124 752
PmlI CAC'GTG
Cuts at: 0 743 876
Size: 743 133
PpuMI rG'GwC_Cy
Cuts at: 0 350 876
Size: 350 526
PspOMI G'GGCC_C
Cuts at: 0 96 408 554 699 876
Size: 96 312 146 145 177
Fragments arranged by size:
312 177 146 145 96
PstI C_TGCA'G
Cuts at: 0 635 876
Size: 635 241
SacII CC_GC'GG
Cuts at: 0 39 201 876
Size: 39 162 675
Fragments arranged by size:
675 162 39
SanDI GG'GwC_CC
Cuts at: 0 350 876
Size: 350 526
ScaI AGT'ACT
Cuts at: 0 790 876
Size: 790 86
SfcI C'TryA_G
Cuts at: 0 631 876
Size: 631 245
SfoI GGC'GCC
Cuts at: 0 20 759 876
Size: 20 739 117
Fragments arranged by size:
739 117 20
SmaI CCC'GGG
Cuts at: 0 95 355 876
Size: 95 260 521
Fragments arranged by size:
521 260 95
SmlI C'TyrA_G
Cuts at: 0 694 876
Size: 694 182
StyI C'CwwG_G
Cuts at: 0 56 876
Size: 56 820
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 876
Size: 700 176
TatI w'GTAC_w
Cuts at: 0 788 876
Size: 788 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 577 609 876
Size: 577 32 267
Fragments arranged by size:
577 267 32
XmaI C'CCGG_G
Cuts at: 0 93 353 876
Size: 93 260 523
Fragments arranged by size:
523 260 93
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI AvaI BanI
BanII BbeI BglI BlpI Bme1580I BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstXI BtgI BtgZI EaeI
EagI Eco57MI EcoO109I HaeII Hin4I KasI MspA1I NaeI
NarI NcoI NgoMIV NotI PasI PfoI PmlI PpuMI
PspOMI PstI SacII SanDI ScaI SfcI SfoI SmaI
SmlI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NdeI NheI NruI NsiI NspI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI