Restriction map for
pFN21AE0132
(Linear) (Six-base) MAPSORT of: KIEE0132.seq Check: 9248 from: 1 to: 738
KIEE0132.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 738
Size: 571 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 738
Size: 32 706
AlwNI CAG_nnn'CTG
Cuts at: 0 627 738
Size: 627 111
ApoI r'AATT_y
Cuts at: 0 206 738
Size: 206 532
AvaI C'yCGr_G
Cuts at: 0 256 738
Size: 256 482
BbvCI CC'TCA_GC
Cuts at: 0 518 738
Size: 518 220
Bme1580I G_kGCm'C
Cuts at: 0 297 738
Size: 297 441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 700 738
Size: 229 471 38
Fragments arranged by size:
471 229 38
Bpu10I CC'TnA_GC
Cuts at: 0 518 738
Size: 518 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 738
Size: 454 284
BsaHI Gr'CG_yC
Cuts at: 0 351 738
Size: 351 387
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 500 738
Size: 470 30 238
Fragments arranged by size:
470 238 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 697 738
Size: 469 228 41
Fragments arranged by size:
469 228 41
BsiEI CG_ry'CG
Cuts at: 0 672 738
Size: 672 66
BsiHKAI G_wGCw'C
Cuts at: 0 272 689 738
Size: 272 417 49
Fragments arranged by size:
417 272 49
BsmI GAATG_Cn'
Cuts at: 0 68 519 738
Size: 68 451 219
Fragments arranged by size:
451 219 68
Bsp1286I G_dGCh'C
Cuts at: 0 272 297 689 738
Size: 272 25 392 49
Fragments arranged by size:
392 272 49 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 569 738
Size: 569 169
BsrDI GCAATG_nn'
Cuts at: 0 311 738
Size: 311 427
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 738
Size: 387 351
BtgI C'CryG_G
Cuts at: 0 263 719 738
Size: 263 456 19
Fragments arranged by size:
456 263 19
BtsI GCAGTG_nn'
Cuts at: 0 16 738
Size: 16 722
ClaI AT'CG_AT
Cuts at: 0 191 738
Size: 191 547
DraI TTT'AAA
Cuts at: 0 408 738
Size: 408 330
EaeI y'GGCC_r
Cuts at: 0 437 584 629 738
Size: 437 147 45 109
Fragments arranged by size:
437 147 109 45
EarI CTCTTCn'nnn_
Cuts at: 0 530 728 738
Size: 530 198 10
Fragments arranged by size:
530 198 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 433 738
Size: 433 305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 571 700 738
Size: 229 342 129 38
Fragments arranged by size:
342 229 129 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 738
Size: 658 80
EcoO109I rG'GnC_Cy
Cuts at: 0 651 738
Size: 651 87
HincII GTy'rAC
Cuts at: 0 489 738
Size: 489 249
HindIII A'AGCT_T
Cuts at: 0 283 738
Size: 283 455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 509 738
Size: 482 27 229
Fragments arranged by size:
482 229 27
MscI TGG'CCA
Cuts at: 0 586 631 738
Size: 586 45 107
Fragments arranged by size:
586 107 45
MslI CAynn'nnrTG
Cuts at: 0 175 738
Size: 175 563
NcoI C'CATG_G
Cuts at: 0 263 719 738
Size: 263 456 19
Fragments arranged by size:
456 263 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 738
Size: 627 111
PstI C_TGCA'G
Cuts at: 0 11 21 566 701 708 738
Size: 11 10 545 135 7 30
Fragments arranged by size:
545 135 30 11 10 7
SexAI A'CCwGG_T
Cuts at: 0 620 738
Size: 620 118
SfcI C'TryA_G
Cuts at: 0 7 17 562 697 704 738
Size: 7 10 545 135 7 34
Fragments arranged by size:
545 135 34 10 7 7
SmaI CCC'GGG
Cuts at: 0 258 738
Size: 258 480
SmlI C'TyrA_G
Cuts at: 0 469 738
Size: 469 269
StyI C'CwwG_G
Cuts at: 0 263 659 719 738
Size: 263 396 60 19
Fragments arranged by size:
396 263 60 19
TatI w'GTAC_w
Cuts at: 0 446 738
Size: 446 292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 738
Size: 447 291
XmaI C'CCGG_G
Cuts at: 0 256 738
Size: 256 482
XmnI GAAnn'nnTTC
Cuts at: 0 103 286 738
Size: 103 183 452
Fragments arranged by size:
452 183 103
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AvaI BbvCI Bme1580I BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BstXI BtgI BtsI ClaI DraI
EaeI EarI EciI Eco57MI EcoNI EcoO109I HincII HindIII
MmeI MscI MslI NcoI PflMI PstI SexAI SfcI
SmaI SmlI StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BsaI BsaAI BsaBI BsaWI BseYI
BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI ZraI