Restriction map for
pFN21AB9997
(Linear) (Six-base) MAPSORT of: KIBB9997.seq Check: 3433 from: 1 to: 3519
KIBB9997.orf
With 182 enzymes: SgfI *
March 22, 2012 15:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3404 3519
Size: 3404 115
AccI GT'mk_AC
Cuts at: 0 1657 3519
Size: 1657 1862
Acc65I G'GTAC_C
Cuts at: 0 2511 3519
Size: 2511 1008
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 482 2071 2767 2971 3519
Size: 473 9 1589 696 204 548
Fragments arranged by size:
1589 696 548 473 204 9
AfeI AGC'GCT
Cuts at: 0 15 2056 3519
Size: 15 2041 1463
Fragments arranged by size:
2041 1463 15
AflII C'TTAA_G
Cuts at: 0 1341 2071 3519
Size: 1341 730 1448
Fragments arranged by size:
1448 1341 730
AflIII A'CryG_T
Cuts at: 0 1786 2696 2897 3519
Size: 1786 910 201 622
Fragments arranged by size:
1786 910 622 201
AleI CACnn'nnGTG
Cuts at: 0 1480 3519
Size: 1480 2039
AlwNI CAG_nnn'CTG
Cuts at: 0 981 1125 1456 2172 3435 3519
Size: 981 144 331 716 1263 84
Fragments arranged by size:
1263 981 716 331 144 84
ApaI G_GGCC'C
Cuts at: 0 451 3519
Size: 451 3068
ApoI r'AATT_y
Cuts at: 0 1677 1867 2145 2220 2281 3220 3519
Size: 1677 190 278 75 61 939 299
Fragments arranged by size:
1677 939 299 278 190 75 61
AvaI C'yCGr_G
Cuts at: 0 444 2807 3519
Size: 444 2363 712
Fragments arranged by size:
2363 712 444
BanI G'GyrC_C
Cuts at: 0 35 790 2511 2644 3361 3392 3519
Size: 35 755 1721 133 717 31 127
Fragments arranged by size:
1721 755 717 133 127 35 31
BanII G_rGCy'C
Cuts at: 0 451 1265 1517 1970 2330 3519
Size: 451 814 252 453 360 1189
Fragments arranged by size:
1189 814 453 451 360 252
BbeI G_GCGC'C
Cuts at: 0 39 3519
Size: 39 3480
BbsI GAAGACnn'nnnn_
Cuts at: 0 1291 3519
Size: 1291 2228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 303 337 2665 2699 2703 2737 2806 2840
Size: 303 34 2328 34 4 34 69 34
Cuts at: 2840 3519
Size: 679
Fragments arranged by size:
2328 679 303 69 34 34 34 34
4
BglII A'GATC_T
Cuts at: 0 491 3519
Size: 491 3028
BlpI GC'TnA_GC
Cuts at: 0 10 3519
Size: 10 3509
Bme1580I G_kGCm'C
Cuts at: 0 451 3519
Size: 451 3068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 459 1154 2682 3519
Size: 459 695 1528 837
Fragments arranged by size:
1528 837 695 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2758 3519
Size: 2758 761
Bpu10I CC'TnA_GC
Cuts at: 0 2843 3519
Size: 2843 676
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 3305 3519
Size: 166 3139 214
Fragments arranged by size:
3139 214 166
BsaAI yAC'GTr
Cuts at: 0 1739 3519
Size: 1739 1780
BsaBI GATnn'nnATC
Cuts at: 0 1171 3519
Size: 1171 2348
BsaHI Gr'CG_yC
Cuts at: 0 36 3519
Size: 36 3483
BsaWI w'CCGG_w
Cuts at: 0 2551 2943 3519
Size: 2551 392 576
Fragments arranged by size:
2551 576 392
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 411 441 727 757 3519
Size: 411 30 286 30 2762
Fragments arranged by size:
2762 411 286 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 2671 3262 3519
Size: 19 2652 591 257
Fragments arranged by size:
2652 591 257 19
BseYI C'CCAG_C
Cuts at: 0 183 3519
Size: 183 3336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 975 2785 3519
Size: 536 439 1810 734
Fragments arranged by size:
1810 734 536 439
BsiEI CG_ry'CG
Cuts at: 0 918 3519
Size: 918 2601
BsiHKAI G_wGCw'C
Cuts at: 0 1216 1265 1517 1970 3108 3519
Size: 1216 49 252 453 1138 411
Fragments arranged by size:
1216 1138 453 411 252 49
BsmI GAATG_Cn'
Cuts at: 0 1800 3519
Size: 1800 1719
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1777 3519
Size: 1777 1742
Bsp1286I G_dGCh'C
Cuts at: 0 451 1216 1265 1517 1970 2330 3108 3519
Size: 451 765 49 252 453 360 778 411
Fragments arranged by size:
778 765 453 451 411 360 252 49
BspHI T'CATG_A
Cuts at: 0 3332 3519
Size: 3332 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 880 2910 3404 3519
Size: 880 2030 494 115
Fragments arranged by size:
2030 880 494 115
BsrBI CCG'CTC
Cuts at: 0 995 2864 3377 3519
Size: 995 1869 513 142
Fragments arranged by size:
1869 995 513 142
BsrDI GCAATG_nn'
Cuts at: 0 1495 2975 3122 3519
Size: 1495 1480 147 397
Fragments arranged by size:
1495 1480 397 147
BsrFI r'CCGG_y
Cuts at: 0 3425 3519
Size: 3425 94
BssSI C'ACGA_G
Cuts at: 0 1478 3519
Size: 1478 2041
BstAPI GCAn_nnn'nTGC
Cuts at: 0 49 3519
Size: 49 3470
BstEII G'GTnAC_C
Cuts at: 0 710 3519
Size: 710 2809
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1467 2508 3519
Size: 1467 1041 1011
Fragments arranged by size:
1467 1041 1011
BstYI r'GATC_y
Cuts at: 0 491 3139 3519
Size: 491 2648 380
Fragments arranged by size:
2648 491 380
BtgI C'CryG_G
Cuts at: 0 2640 2891 3410 3519
Size: 2640 251 519 109
Fragments arranged by size:
2640 519 251 109
BtsI GCAGTG_nn'
Cuts at: 0 240 2209 3519
Size: 240 1969 1310
Fragments arranged by size:
1969 1310 240
ClaI AT'CG_AT
Cuts at: 0 323 3519
Size: 323 3196
DraI TTT'AAA
Cuts at: 0 1236 2219 2613 3519
Size: 1236 983 394 906
Fragments arranged by size:
1236 983 906 394
DraIII CAC_nnn'GTG
Cuts at: 0 1258 1296 3519
Size: 1258 38 2223
Fragments arranged by size:
2223 1258 38
DrdI GACnn_nn'nnGTC
Cuts at: 0 526 3519
Size: 526 2993
EaeI y'GGCC_r
Cuts at: 0 606 2405 2879 3257 3519
Size: 606 1799 474 378 262
Fragments arranged by size:
1799 606 474 378 262
EarI CTCTTCn'nnn_
Cuts at: 0 402 458 1775 2765 3519
Size: 402 56 1317 990 754
Fragments arranged by size:
1317 990 754 402 56
EcoICRI GAG'CTC
Cuts at: 0 1263 1515 1968 3519
Size: 1263 252 453 1551
Fragments arranged by size:
1551 1263 453 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 482 2071 2758 2767 2971 3519
Size: 473 9 1589 687 9 204 548
Fragments arranged by size:
1589 687 548 473 204 9 9
EcoO109I rG'GnC_Cy
Cuts at: 0 115 142 448 1052 1453 3519
Size: 115 27 306 604 401 2066
Fragments arranged by size:
2066 604 401 306 115 27
EcoRV GAT'ATC
Cuts at: 0 321 3519
Size: 321 3198
HaeII r_GCGC'y
Cuts at: 0 17 39 2058 2320 3519
Size: 17 22 2019 262 1199
Fragments arranged by size:
2019 1199 262 22 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 316 348 515 547 624 656
Size: 144 32 140 32 167 32 77 32
Cuts at: 656 2124 2156 2842 2874 3124 3156 3519
Size: 1468 32 686 32 250 32 363
Fragments arranged by size:
1468 686 363 250 167 144 140 77
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 35 3519
Size: 35 3484
KpnI G_GTAC'C
Cuts at: 0 2515 3519
Size: 2515 1004
MfeI C'AATT_G
Cuts at: 0 692 3519
Size: 692 2827
MluI A'CGCG_T
Cuts at: 0 1786 3519
Size: 1786 1733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1741 3047 3519
Size: 903 838 1306 472
Fragments arranged by size:
1306 903 838 472
MscI TGG'CCA
Cuts at: 0 608 2407 2881 3259 3519
Size: 608 1799 474 378 260
Fragments arranged by size:
1799 608 474 378 260
MslI CAynn'nnrTG
Cuts at: 0 1145 1480 2428 2650 3331 3367 3519
Size: 1145 335 948 222 681 36 152
Fragments arranged by size:
1145 948 681 335 222 152 36
MspA1I CmG'CkG
Cuts at: 0 480 2169 3096 3519
Size: 480 1689 927 423
Fragments arranged by size:
1689 927 480 423
NarI GG'CG_CC
Cuts at: 0 36 3519
Size: 36 3483
NcoI C'CATG_G
Cuts at: 0 2640 3519
Size: 2640 879
NdeI CA'TA_TG
Cuts at: 0 1107 3519
Size: 1107 2412
NruI TCG'CGA
Cuts at: 0 3115 3519
Size: 3115 404
NspI r_CATG'y
Cuts at: 0 351 1903 2700 2901 3450 3519
Size: 351 1552 797 201 549 69
Fragments arranged by size:
1552 797 549 351 201 69
PciI A'CATG_T
Cuts at: 0 2696 2897 3519
Size: 2696 201 622
Fragments arranged by size:
2696 622 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 3519
Size: 315 3204
PfoI T'CCnGG_A
Cuts at: 0 750 1961 2183 2668 3519
Size: 750 1211 222 485 851
Fragments arranged by size:
1211 851 750 485 222
PpuMI rG'GwC_Cy
Cuts at: 0 142 1052 3519
Size: 142 910 2467
Fragments arranged by size:
2467 910 142
PspOMI G'GGCC_C
Cuts at: 0 447 3519
Size: 447 3072
PstI C_TGCA'G
Cuts at: 0 702 775 1370 1697 3519
Size: 702 73 595 327 1822
Fragments arranged by size:
1822 702 595 327 73
PvuII CAG'CTG
Cuts at: 0 480 2169 3096 3519
Size: 480 1689 927 423
Fragments arranged by size:
1689 927 480 423
SacI G_AGCT'C
Cuts at: 0 1265 1517 1970 3519
Size: 1265 252 453 1549
Fragments arranged by size:
1549 1265 453 252
SapI GCTCTTCn'nnn_
Cuts at: 0 458 3519
Size: 458 3061
ScaI AGT'ACT
Cuts at: 0 1025 3519
Size: 1025 2494
SfcI C'TryA_G
Cuts at: 0 698 771 846 1366 1658 1693 1979 3144
Size: 698 73 75 520 292 35 286 1165
Cuts at: 3144 3285 3519
Size: 141 234
Fragments arranged by size:
1165 698 520 292 286 234 141 75
73 35
SfoI GGC'GCC
Cuts at: 0 37 3519
Size: 37 3482
SmaI CCC'GGG
Cuts at: 0 2809 3519
Size: 2809 710
SmlI C'TyrA_G
Cuts at: 0 1341 2071 3519
Size: 1341 730 1448
Fragments arranged by size:
1448 1341 730
SphI G_CATG'C
Cuts at: 0 1903 3450 3519
Size: 1903 1547 69
Fragments arranged by size:
1903 1547 69
StuI AGG'CCT
Cuts at: 0 472 1190 2095 3519
Size: 472 718 905 1424
Fragments arranged by size:
1424 905 718 472
StyI C'CwwG_G
Cuts at: 0 2234 2640 3519
Size: 2234 406 879
Fragments arranged by size:
2234 879 406
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 932 1766 3519
Size: 932 834 1753
Fragments arranged by size:
1753 932 834
TatI w'GTAC_w
Cuts at: 0 1023 1705 2332 3519
Size: 1023 682 627 1187
Fragments arranged by size:
1187 1023 682 627
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 411 443 3519
Size: 411 32 3076
Fragments arranged by size:
3076 411 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2505 2889 3519
Size: 2505 384 630
Fragments arranged by size:
2505 630 384
XmaI C'CCGG_G
Cuts at: 0 2807 3519
Size: 2807 712
XmnI GAAnn'nnTTC
Cuts at: 0 2932 3519
Size: 2932 587
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BcgI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV HaeII Hin4I KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NruI NspI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SapI ScaI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AgeI AhdI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglI BmgBI BmrI BmtI BpuEI BsiWI BspEI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI EagI
EciI EcoNI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI NaeI NgoMIV NheI NotI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI