Restriction map for
pFN21AB9982
(Linear) (Six-base) MAPSORT of: KIBB9982.seq Check: 3153 from: 1 to: 2127
KIBB9982.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 680 2127
Size: 680 1447
AatII G_ACGT'C
Cuts at: 0 29 2127
Size: 29 2098
AccI GT'mk_AC
Cuts at: 0 63 212 1201 2127
Size: 63 149 989 926
Fragments arranged by size:
989 926 149 63
Acc65I G'GTAC_C
Cuts at: 0 1144 2127
Size: 1144 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1191 2084 2127
Size: 1191 893 43
Fragments arranged by size:
1191 893 43
AfeI AGC'GCT
Cuts at: 0 889 2127
Size: 889 1238
AflIII A'CryG_T
Cuts at: 0 914 2127
Size: 914 1213
AhdI GACnn_n'nnGTC
Cuts at: 0 2109 2127
Size: 2109 18
AlwNI CAG_nnn'CTG
Cuts at: 0 621 2127
Size: 621 1506
ApaI G_GGCC'C
Cuts at: 0 1014 2127
Size: 1014 1113
ApaLI G'TGCA_C
Cuts at: 0 414 1099 2127
Size: 414 685 1028
Fragments arranged by size:
1028 685 414
ApoI r'AATT_y
Cuts at: 0 337 445 2127
Size: 337 108 1682
Fragments arranged by size:
1682 337 108
BamHI G'GATC_C
Cuts at: 0 777 922 2116 2127
Size: 777 145 1194 11
Fragments arranged by size:
1194 777 145 11
BanI G'GyrC_C
Cuts at: 0 158 1144 2127
Size: 158 986 983
Fragments arranged by size:
986 983 158
BanII G_rGCy'C
Cuts at: 0 1014 1939 2014 2127
Size: 1014 925 75 113
Fragments arranged by size:
1014 925 113 75
BbeI G_GCGC'C
Cuts at: 0 162 2127
Size: 162 1965
BbsI GAAGACnn'nnnn_
Cuts at: 0 1853 2127
Size: 1853 274
BbvCI CC'TCA_GC
Cuts at: 0 397 1799 2127
Size: 397 1402 328
Fragments arranged by size:
1402 397 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1303 1337 2127
Size: 1303 34 790
Fragments arranged by size:
1303 790 34
BclI T'GATC_A
Cuts at: 0 767 2127
Size: 767 1360
BglI GCCn_nnn'nGGC
Cuts at: 0 83 1573 2127
Size: 83 1490 554
Fragments arranged by size:
1490 554 83
BglII A'GATC_T
Cuts at: 0 1118 1748 2127
Size: 1118 630 379
Fragments arranged by size:
1118 630 379
BlpI GC'TnA_GC
Cuts at: 0 1966 2127
Size: 1966 161
Bme1580I G_kGCm'C
Cuts at: 0 418 1004 1014 1103 1195 2127
Size: 418 586 10 89 92 932
Fragments arranged by size:
932 586 418 92 89 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 405 1008 2127
Size: 333 72 603 1119
Fragments arranged by size:
1119 603 333 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 270 2127
Size: 270 1857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1084 1494 2127
Size: 34 1050 410 633
Fragments arranged by size:
1050 633 410 34
Bpu10I CC'TnA_GC
Cuts at: 0 397 1044 1799 2127
Size: 397 647 755 328
Fragments arranged by size:
755 647 397 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1941 2127
Size: 304 1637 186
Fragments arranged by size:
1637 304 186
BsaI GGTCTCn'nnnn_
Cuts at: 0 1174 2127
Size: 1174 953
BsaHI Gr'CG_yC
Cuts at: 0 26 159 2127
Size: 26 133 1968
Fragments arranged by size:
1968 133 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 2127
Size: 601 30 1496
Fragments arranged by size:
1496 601 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 814 1240 1405 1474 1492 1497 1795
Size: 617 197 426 165 69 18 5 298
Cuts at: 1795 2058 2061 2064 2127
Size: 263 3 3 63
Fragments arranged by size:
617 426 298 263 197 165 69 63
18 5 3 3
BseYI C'CCAG_C
Cuts at: 0 133 2092 2127
Size: 133 1959 35
Fragments arranged by size:
1959 133 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 2127
Size: 652 1475
BsiHKAI G_wGCw'C
Cuts at: 0 418 1103 2014 2127
Size: 418 685 911 113
Fragments arranged by size:
911 685 418 113
BsmI GAATG_Cn'
Cuts at: 0 1401 2127
Size: 1401 726
Bsp1286I G_dGCh'C
Cuts at: 0 418 1004 1014 1103 1195 1939 2014 2127
Size: 418 586 10 89 92 744 75 113
Fragments arranged by size:
744 586 418 113 92 89 75 10
BspHI T'CATG_A
Cuts at: 0 950 2127
Size: 950 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 2127
Size: 680 1447
BsrBI CCG'CTC
Cuts at: 0 1068 2127
Size: 1068 1059
BsrDI GCAATG_nn'
Cuts at: 0 408 2127
Size: 408 1719
BssSI C'ACGA_G
Cuts at: 0 629 1139 2127
Size: 629 510 988
Fragments arranged by size:
988 629 510
BstEII G'GTnAC_C
Cuts at: 0 370 2127
Size: 370 1757
BstXI CCAn_nnnn'nTGG
Cuts at: 0 517 2127
Size: 517 1610
BstYI r'GATC_y
Cuts at: 0 650 698 777 922 1118 1382 1725 1748
Size: 650 48 79 145 196 264 343 23
Cuts at: 1748 2116 2127
Size: 368 11
Fragments arranged by size:
650 368 343 264 196 145 79 48
23 11
BstZ17I GTA'TAC
Cuts at: 0 1202 2127
Size: 1202 925
BtgI C'CryG_G
Cuts at: 0 177 581 2127
Size: 177 404 1546
Fragments arranged by size:
1546 404 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1427 2127
Size: 1427 700
BtsI GCAGTG_nn'
Cuts at: 0 622 2127
Size: 622 1505
DrdI GACnn_nn'nnGTC
Cuts at: 0 379 2127
Size: 379 1748
EaeI y'GGCC_r
Cuts at: 0 204 1574 2127
Size: 204 1370 553
Fragments arranged by size:
1370 553 204
EarI CTCTTCn'nnn_
Cuts at: 0 832 1451 2019 2035 2127
Size: 832 619 568 16 92
Fragments arranged by size:
832 619 568 92 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 580 2127
Size: 580 1547
EcoICRI GAG'CTC
Cuts at: 0 2012 2127
Size: 2012 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1084 1191 1494 2084 2127
Size: 34 1050 107 303 590 43
Fragments arranged by size:
1050 590 303 107 43 34
EcoO109I rG'GnC_Cy
Cuts at: 0 300 1010 2127
Size: 300 710 1117
Fragments arranged by size:
1117 710 300
EcoRI G'AATT_C
Cuts at: 0 445 2127
Size: 445 1682
HaeII r_GCGC'y
Cuts at: 0 162 753 891 2127
Size: 162 591 138 1236
Fragments arranged by size:
1236 591 162 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 578 610 1105 1137 1676 1708 1981 1996
Size: 578 32 495 32 539 32 273 15
Cuts at: 1996 2013 2028 2095 2127
Size: 17 15 67 32
Fragments arranged by size:
578 539 495 273 67 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 0 64 143 854 1131 1254 2127
Size: 64 79 711 277 123 873
Fragments arranged by size:
873 711 277 123 79 64
HpaI GTT'AAC
Cuts at: 0 143 2127
Size: 143 1984
KasI G'GCGC_C
Cuts at: 0 158 2127
Size: 158 1969
KpnI G_GTAC'C
Cuts at: 0 1148 2127
Size: 1148 979
MscI TGG'CCA
Cuts at: 0 206 1576 2127
Size: 206 1370 551
Fragments arranged by size:
1370 551 206
MslI CAynn'nnrTG
Cuts at: 0 484 674 2127
Size: 484 190 1453
Fragments arranged by size:
1453 484 190
MspA1I CmG'CkG
Cuts at: 0 351 1062 2127
Size: 351 711 1065
Fragments arranged by size:
1065 711 351
NarI GG'CG_CC
Cuts at: 0 159 2127
Size: 159 1968
NcoI C'CATG_G
Cuts at: 0 177 2127
Size: 177 1950
NspI r_CATG'y
Cuts at: 0 918 1665 2127
Size: 918 747 462
Fragments arranged by size:
918 747 462
PciI A'CATG_T
Cuts at: 0 914 2127
Size: 914 1213
PfoI T'CCnGG_A
Cuts at: 0 605 1385 2127
Size: 605 780 742
Fragments arranged by size:
780 742 605
PpuMI rG'GwC_Cy
Cuts at: 0 300 2127
Size: 300 1827
PspOMI G'GGCC_C
Cuts at: 0 1010 2127
Size: 1010 1117
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 2127
Size: 1294 32 801
Fragments arranged by size:
1294 801 32
PstI C_TGCA'G
Cuts at: 0 617 2127
Size: 617 1510
PvuII CAG'CTG
Cuts at: 0 351 2127
Size: 351 1776
SacI G_AGCT'C
Cuts at: 0 2014 2127
Size: 2014 113
SalI G'TCGA_C
Cuts at: 0 62 2127
Size: 62 2065
SapI GCTCTTCn'nnn_
Cuts at: 0 2019 2127
Size: 2019 108
ScaI AGT'ACT
Cuts at: 0 716 2085 2127
Size: 716 1369 42
Fragments arranged by size:
1369 716 42
SfcI C'TryA_G
Cuts at: 0 613 2127
Size: 613 1514
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1573 2127
Size: 1573 554
SfoI GGC'GCC
Cuts at: 0 160 2127
Size: 160 1967
SmlI C'TyrA_G
Cuts at: 0 283 1956 2127
Size: 283 1673 171
Fragments arranged by size:
1673 283 171
StuI AGG'CCT
Cuts at: 0 538 2127
Size: 538 1589
StyI C'CwwG_G
Cuts at: 0 177 833 996 1577 2127
Size: 177 656 163 581 550
Fragments arranged by size:
656 581 550 177 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 519 523 2127
Size: 519 4 1604
Fragments arranged by size:
1604 519 4
TatI w'GTAC_w
Cuts at: 0 714 2083 2127
Size: 714 1369 44
Fragments arranged by size:
1369 714 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 601 633 2127
Size: 431 32 138 32 1494
Fragments arranged by size:
1494 431 138 32 32
ZraI GAC'GTC
Cuts at: 0 27 2127
Size: 27 2100
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AlwNI ApaI ApaLI ApoI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII HpaI KasI KpnI
MscI MslI MspA1I NarI NcoI NspI PciI PfoI
PpuMI PspOMI PsrI PstI PvuII SacI SalI SapI
ScaI SfcI SfiI SfoI SmlI StuI StyI TaqII
TatI TstI ZraI
Enzymes that do not cut:
AclI AflII AgeI AleI AlfI AloI AscI AseI
AsiSI AvaI AvrII BaeI BciVI BfrBI BmgBI BmtI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstAPI BstBI Bsu36I ClaI CspCI DraI
DraIII EagI EcoNI EcoRV FalI FseI FspI FspAI
HindIII MfeI MluI MmeI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PvuI RsrII SacII SanDI
SbfI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI