Restriction map for
pFN21AB9976
(Linear) (Six-base) MAPSORT of: KIBB9976.seq Check: 4078 from: 1 to: 2121
KIBB9976.orf
With 182 enzymes: SgfI *
July 3, 2012 16:40 ..
AccI GT'mk_AC
Cuts at: 0 1077 2121
Size: 1077 1044
AfeI AGC'GCT
Cuts at: 0 1996 2121
Size: 1996 125
AleI CACnn'nnGTG
Cuts at: 0 53 2121
Size: 53 2068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2027 2121
Size: 2027 94
ApaI G_GGCC'C
Cuts at: 0 423 580 604 776 791 2121
Size: 423 157 24 172 15 1330
Fragments arranged by size:
1330 423 172 157 24 15
ApoI r'AATT_y
Cuts at: 0 934 1249 1888 2121
Size: 934 315 639 233
Fragments arranged by size:
934 639 315 233
AvaI C'yCGr_G
Cuts at: 0 5 150 414 484 573 579 663 2121
Size: 5 145 264 70 89 6 84 1458
Fragments arranged by size:
1458 264 145 89 84 70 6 5
BamHI G'GATC_C
Cuts at: 0 1809 2121
Size: 1809 312
BanI G'GyrC_C
Cuts at: 0 489 1769 2121
Size: 489 1280 352
Fragments arranged by size:
1280 489 352
BanII G_rGCy'C
Cuts at: 0 423 580 604 722 776 791 2121
Size: 423 157 24 118 54 15 1330
Fragments arranged by size:
1330 423 157 118 54 24 15
BbeI G_GCGC'C
Cuts at: 0 493 1773 2121
Size: 493 1280 348
Fragments arranged by size:
1280 493 348
BbsI GAAGACnn'nnnn_
Cuts at: 0 1366 2121
Size: 1366 755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 455 489 981 1015 2121
Size: 455 34 492 34 1106
Fragments arranged by size:
1106 492 455 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 77 161 642 786 1799 2121
Size: 77 84 481 144 1013 322
Fragments arranged by size:
1013 481 322 144 84 77
BglII A'GATC_T
Cuts at: 0 812 1225 1611 2121
Size: 812 413 386 510
Fragments arranged by size:
812 510 413 386
Bme1580I G_kGCm'C
Cuts at: 0 423 580 604 776 791 2121
Size: 423 157 24 172 15 1330
Fragments arranged by size:
1330 423 172 157 24 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 831 2121
Size: 831 1290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 884 2045 2121
Size: 366 518 1161 76
Fragments arranged by size:
1161 518 366 76
Bpu10I CC'TnA_GC
Cuts at: 0 167 335 593 2121
Size: 167 168 258 1528
Fragments arranged by size:
1528 258 168 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 2121
Size: 873 1248
BsaBI GATnn'nnATC
Cuts at: 0 1577 2121
Size: 1577 544
BsaHI Gr'CG_yC
Cuts at: 0 490 1770 2121
Size: 490 1280 351
Fragments arranged by size:
1280 490 351
BsaWI w'CCGG_w
Cuts at: 0 17 2121
Size: 17 2104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 1305 1335 2121
Size: 516 30 759 30 786
Fragments arranged by size:
786 759 516 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 524 820 870 1313 1421 1717 1859
Size: 74 450 296 50 443 108 296 142
Cuts at: 1859 2121
Size: 262
Fragments arranged by size:
450 443 296 296 262 142 108 74
50
BseYI C'CCAG_C
Cuts at: 0 1442 2121
Size: 1442 679
BsiEI CG_ry'CG
Cuts at: 0 72 798 2121
Size: 72 726 1323
Fragments arranged by size:
1323 726 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1614 2121
Size: 1614 507
Bsp1286I G_dGCh'C
Cuts at: 0 423 580 604 722 776 791 2121
Size: 423 157 24 118 54 15 1330
Fragments arranged by size:
1330 423 157 118 54 24 15
BspEI T'CCGG_A
Cuts at: 0 17 2121
Size: 17 2104
BspHI T'CATG_A
Cuts at: 0 1581 2121
Size: 1581 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 2121
Size: 921 1200
BsrBI CCG'CTC
Cuts at: 0 161 352 2121
Size: 161 191 1769
Fragments arranged by size:
1769 191 161
BsrDI GCAATG_nn'
Cuts at: 0 1856 2121
Size: 1856 265
BstXI CCAn_nnnn'nTGG
Cuts at: 0 55 2121
Size: 55 2066
BstYI r'GATC_y
Cuts at: 0 812 1225 1611 1809 1863 2121
Size: 812 413 386 198 54 258
Fragments arranged by size:
812 413 386 258 198 54
Bsu36I CC'TnA_GG
Cuts at: 0 536 2121
Size: 536 1585
BtgI C'CryG_G
Cuts at: 0 72 137 563 723 1813 2121
Size: 72 65 426 160 1090 308
Fragments arranged by size:
1090 426 308 160 72 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 900 1491 2121
Size: 900 591 630
Fragments arranged by size:
900 630 591
BtsI GCAGTG_nn'
Cuts at: 0 1299 2121
Size: 1299 822
DraI TTT'AAA
Cuts at: 0 834 2121
Size: 834 1287
EaeI y'GGCC_r
Cuts at: 0 69 684 795 2121
Size: 69 615 111 1326
Fragments arranged by size:
1326 615 111 69
EagI C'GGCC_G
Cuts at: 0 69 795 2121
Size: 69 726 1326
Fragments arranged by size:
1326 726 69
EarI CTCTTCn'nnn_
Cuts at: 0 321 1713 1721 2081 2121
Size: 321 1392 8 360 40
Fragments arranged by size:
1392 360 321 40 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 89 548 1856 2121
Size: 89 459 1308 265
Fragments arranged by size:
1308 459 265 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 884 2045 2121
Size: 366 518 1161 76
Fragments arranged by size:
1161 518 366 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1901 2121
Size: 1901 220
EcoO109I rG'GnC_Cy
Cuts at: 0 142 340 419 420 519 589 600 772
Size: 142 198 79 1 99 70 11 172
Cuts at: 772 773 2017 2035 2050 2121
Size: 1 1244 18 15 71
Fragments arranged by size:
1244 198 172 142 99 79 71 70
18 15 11 1 1
EcoRI G'AATT_C
Cuts at: 0 934 2121
Size: 934 1187
EcoRV GAT'ATC
Cuts at: 0 921 2121
Size: 921 1200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1390 2121
Size: 1390 731
HaeII r_GCGC'y
Cuts at: 0 493 1773 1998 2121
Size: 493 1280 225 123
Fragments arranged by size:
1280 493 225 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1305 1308 1337 1340 1725 1757 2121
Size: 1305 3 29 3 385 32 364
Fragments arranged by size:
1305 385 364 32 29 3 3
HincII GTy'rAC
Cuts at: 0 1744 2121
Size: 1744 377
HindIII A'AGCT_T
Cuts at: 0 1012 2121
Size: 1012 1109
KasI G'GCGC_C
Cuts at: 0 489 1769 2121
Size: 489 1280 352
Fragments arranged by size:
1280 489 352
MfeI C'AATT_G
Cuts at: 0 1690 2121
Size: 1690 431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1139 2121
Size: 475 664 982
Fragments arranged by size:
982 664 475
MslI CAynn'nnrTG
Cuts at: 0 53 885 1831 2121
Size: 53 832 946 290
Fragments arranged by size:
946 832 290 53
MspA1I CmG'CkG
Cuts at: 0 74 515 565 725 2121
Size: 74 441 50 160 1396
Fragments arranged by size:
1396 441 160 74 50
NarI GG'CG_CC
Cuts at: 0 490 1770 2121
Size: 490 1280 351
Fragments arranged by size:
1280 490 351
NcoI C'CATG_G
Cuts at: 0 137 2121
Size: 137 1984
NotI GC'GGCC_GC
Cuts at: 0 69 795 2121
Size: 69 726 1326
Fragments arranged by size:
1326 726 69
NspI r_CATG'y
Cuts at: 0 1108 1830 1986 2121
Size: 1108 722 156 135
Fragments arranged by size:
1108 722 156 135
PfoI T'CCnGG_A
Cuts at: 0 354 2121
Size: 354 1767
PpuMI rG'GwC_Cy
Cuts at: 0 142 340 519 2035 2121
Size: 142 198 179 1516 86
Fragments arranged by size:
1516 198 179 142 86
PspOMI G'GGCC_C
Cuts at: 0 419 576 600 772 787 2121
Size: 419 157 24 172 15 1334
Fragments arranged by size:
1334 419 172 157 24 15
PstI C_TGCA'G
Cuts at: 0 468 2121
Size: 468 1653
SacII CC_GC'GG
Cuts at: 0 75 566 726 2121
Size: 75 491 160 1395
Fragments arranged by size:
1395 491 160 75
SanDI GG'GwC_CC
Cuts at: 0 340 519 2121
Size: 340 179 1602
Fragments arranged by size:
1602 340 179
SfcI C'TryA_G
Cuts at: 0 464 1229 2121
Size: 464 765 892
Fragments arranged by size:
892 765 464
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 77 161 2121
Size: 77 84 1960
Fragments arranged by size:
1960 84 77
SfoI GGC'GCC
Cuts at: 0 491 1771 2121
Size: 491 1280 350
Fragments arranged by size:
1280 491 350
SmaI CCC'GGG
Cuts at: 0 152 486 575 581 665 2121
Size: 152 334 89 6 84 1456
Fragments arranged by size:
1456 334 152 89 84 6
SmlI C'TyrA_G
Cuts at: 0 852 2121
Size: 852 1269
SrfI GCCC'GGGC
Cuts at: 0 152 665 2121
Size: 152 513 1456
Fragments arranged by size:
1456 513 152
SspI AAT'ATT
Cuts at: 0 1468 2121
Size: 1468 653
StuI AGG'CCT
Cuts at: 0 861 2121
Size: 861 1260
StyI C'CwwG_G
Cuts at: 0 137 2121
Size: 137 1984
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 513 1080 1825 2014 2121
Size: 513 567 745 189 107
Fragments arranged by size:
745 567 513 189 107
Tth111I GACn'n_nGTC
Cuts at: 0 2033 2121
Size: 2033 88
XbaI T'CTAG_A
Cuts at: 0 1021 2121
Size: 1021 1100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 1155 2121
Size: 1050 105 966
Fragments arranged by size:
1050 966 105
XmaI C'CCGG_G
Cuts at: 0 150 484 573 579 663 2121
Size: 150 334 89 6 84 1458
Fragments arranged by size:
1458 334 150 89 84 6
XmnI GAAnn'nnTTC
Cuts at: 0 1652 2121
Size: 1652 469
Enzymes that do cut:
AccI AfeI AleI AlfI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I
BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI
MfeI MmeI MslI MspA1I NarI NcoI NotI NspI
PfoI PpuMI PspOMI PstI SacII SanDI SfcI SfiI
SfoI SmaI SmlI SrfI SspI StuI StyI TaqII
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI BsaI BsaAI BsgI BsiHKAI BsiWI BsmI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraIII DrdI EcoICRI FseI FspI FspAI HpaI
KpnI MluI MscI NaeI NdeI NgoMIV NheI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SwaI TatI TstI XhoI ZraI