Restriction map for
pFN21AB9966
(Linear) (Six-base) MAPSORT of: KIBB9966.seq Check: 5577 from: 1 to: 2085
KIBB9966.orf
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AccI GT'mk_AC
Cuts at: 0 742 1370 2085
Size: 742 628 715
Fragments arranged by size:
742 715 628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2003 2085
Size: 2003 82
AflII C'TTAA_G
Cuts at: 0 721 2085
Size: 721 1364
AhdI GACnn_n'nnGTC
Cuts at: 0 923 954 2085
Size: 923 31 1131
Fragments arranged by size:
1131 923 31
AleI CACnn'nnGTG
Cuts at: 0 560 2085
Size: 560 1525
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1567 1741 2085
Size: 1567 174 344
Fragments arranged by size:
1567 344 174
ApoI r'AATT_y
Cuts at: 0 486 1955 2085
Size: 486 1469 130
Fragments arranged by size:
1469 486 130
AvaI C'yCGr_G
Cuts at: 0 132 2085
Size: 132 1953
BamHI G'GATC_C
Cuts at: 0 686 2085
Size: 686 1399
BanI G'GyrC_C
Cuts at: 0 1755 1794 2085
Size: 1755 39 291
Fragments arranged by size:
1755 291 39
BanII G_rGCy'C
Cuts at: 0 46 2085
Size: 46 2039
BbsI GAAGACnn'nnnn_
Cuts at: 0 953 2085
Size: 953 1132
BbvCI CC'TCA_GC
Cuts at: 0 730 2085
Size: 730 1355
BfrBI ATG'CAT
Cuts at: 0 1054 2085
Size: 1054 1031
BglII A'GATC_T
Cuts at: 0 227 2085
Size: 227 1858
BlpI GC'TnA_GC
Cuts at: 0 502 1921 2085
Size: 502 1419 164
Fragments arranged by size:
1419 502 164
Bme1580I G_kGCm'C
Cuts at: 0 1799 2085
Size: 1799 286
BmgBI CAC'GTC
Cuts at: 0 1029 2085
Size: 1029 1056
BmrI ACTGGGnnnn_n'
Cuts at: 0 1142 1322 2085
Size: 1142 180 763
Fragments arranged by size:
1142 763 180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 953 2085
Size: 953 1132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 861 2085
Size: 235 626 1224
Fragments arranged by size:
1224 626 235
Bpu10I CC'TnA_GC
Cuts at: 0 34 45 730 1043 2085
Size: 34 11 685 313 1042
Fragments arranged by size:
1042 685 313 34 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 2085
Size: 1774 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 21 2085
Size: 21 2064
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1680 2085
Size: 1680 405
BseYI C'CCAG_C
Cuts at: 0 814 2085
Size: 814 1271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1462 2085
Size: 1462 623
BsiHKAI G_wGCw'C
Cuts at: 0 1610 2085
Size: 1610 475
BsmI GAATG_Cn'
Cuts at: 0 28 144 565 1054 2085
Size: 28 116 421 489 1031
Fragments arranged by size:
1031 489 421 116 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 837 2085
Size: 837 1248
Bsp1286I G_dGCh'C
Cuts at: 0 46 1610 1799 2085
Size: 46 1564 189 286
Fragments arranged by size:
1564 286 189 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 1042 1250 2085
Size: 329 713 208 835
Fragments arranged by size:
835 713 329 208
BsrDI GCAATG_nn'
Cuts at: 0 819 1020 1250 2085
Size: 819 201 230 835
Fragments arranged by size:
835 819 230 201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1422 2085
Size: 1422 663
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1428 1467 1719 2085
Size: 1428 39 252 366
Fragments arranged by size:
1428 366 252 39
BstYI r'GATC_y
Cuts at: 0 62 227 686 931 2085
Size: 62 165 459 245 1154
Fragments arranged by size:
1154 459 245 165 62
Bsu36I CC'TnA_GG
Cuts at: 0 338 2085
Size: 338 1747
BtgI C'CryG_G
Cuts at: 0 642 1556 2085
Size: 642 914 529
Fragments arranged by size:
914 642 529
BtsI GCAGTG_nn'
Cuts at: 0 69 2010 2085
Size: 69 1941 75
Fragments arranged by size:
1941 75 69
EaeI y'GGCC_r
Cuts at: 0 1124 1721 2007 2085
Size: 1124 597 286 78
Fragments arranged by size:
1124 597 286 78
EarI CTCTTCn'nnn_
Cuts at: 0 2022 2085
Size: 2022 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1438 2085
Size: 1438 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 861 2003 2085
Size: 235 626 1142 82
Fragments arranged by size:
1142 626 235 82
EcoO109I rG'GnC_Cy
Cuts at: 0 210 2085
Size: 210 1875
EcoRV GAT'ATC
Cuts at: 0 1074 1266 2085
Size: 1074 192 819
Fragments arranged by size:
1074 819 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 460 506 2085
Size: 460 46 1579
Fragments arranged by size:
1579 460 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 411 443 836 868 909 940
Size: 256 32 123 32 393 32 41 31
Cuts at: 940 941 972 1269 1301 2085
Size: 1 31 297 32 784
Fragments arranged by size:
784 393 297 256 123 41 32 32
32 32 31 31 1
HincII GTy'rAC
Cuts at: 0 743 1026 2085
Size: 743 283 1059
Fragments arranged by size:
1059 743 283
HindIII A'AGCT_T
Cuts at: 0 169 778 2085
Size: 169 609 1307
Fragments arranged by size:
1307 609 169
MfeI C'AATT_G
Cuts at: 0 476 2085
Size: 476 1609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 407 2085
Size: 299 108 1678
Fragments arranged by size:
1678 299 108
MscI TGG'CCA
Cuts at: 0 1126 1723 2009 2085
Size: 1126 597 286 76
Fragments arranged by size:
1126 597 286 76
MslI CAynn'nnrTG
Cuts at: 0 560 824 1144 1292 1471 1537 1723 1807
Size: 560 264 320 148 179 66 186 84
Cuts at: 1807 2085
Size: 278
Fragments arranged by size:
560 320 278 264 186 179 148 84
66
MspA1I CmG'CkG
Cuts at: 0 1428 1498 1584 2085
Size: 1428 70 86 501
Fragments arranged by size:
1428 501 86 70
NcoI C'CATG_G
Cuts at: 0 1556 2085
Size: 1556 529
NsiI A_TGCA'T
Cuts at: 0 1056 2085
Size: 1056 1029
NspI r_CATG'y
Cuts at: 0 1420 2085
Size: 1420 665
PflMI CCAn_nnn'nTGG
Cuts at: 0 1304 2005 2085
Size: 1304 701 80
Fragments arranged by size:
1304 701 80
PpuMI rG'GwC_Cy
Cuts at: 0 210 2085
Size: 210 1875
PvuII CAG'CTG
Cuts at: 0 1428 1498 1584 2085
Size: 1428 70 86 501
Fragments arranged by size:
1428 501 86 70
SalI G'TCGA_C
Cuts at: 0 741 2085
Size: 741 1344
SapI GCTCTTCn'nnn_
Cuts at: 0 2022 2085
Size: 2022 63
SfcI C'TryA_G
Cuts at: 0 963 2085
Size: 963 1122
SmlI C'TyrA_G
Cuts at: 0 721 1789 2085
Size: 721 1068 296
Fragments arranged by size:
1068 721 296
SphI G_CATG'C
Cuts at: 0 1420 2085
Size: 1420 665
StuI AGG'CCT
Cuts at: 0 343 2085
Size: 343 1742
StyI C'CwwG_G
Cuts at: 0 1391 1556 1999 2085
Size: 1391 165 443 86
Fragments arranged by size:
1391 443 165 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1773 2008 2085
Size: 1773 235 77
Fragments arranged by size:
1773 235 77
XbaI T'CTAG_A
Cuts at: 0 666 2085
Size: 666 1419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 649 2002 2085
Size: 649 1353 83
Fragments arranged by size:
1353 649 83
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EciI Eco57MI EcoO109I EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PpuMI PvuII SalI SapI SfcI SmlI
SphI StuI StyI TaqII XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BglI BmtI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XhoI XmaI XmnI ZraI