Restriction map for
pFN21AB9944
(Linear) (Six-base) MAPSORT of: KIBB9944.seq Check: 4067 from: 1 to: 1956
KIBB9944.orf
With 182 enzymes: SgfI *
March 22, 2012 15:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 406 1429 1956
Size: 406 1023 527
Fragments arranged by size:
1023 527 406
AatII G_ACGT'C
Cuts at: 0 508 827 1956
Size: 508 319 1129
Fragments arranged by size:
1129 508 319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1663 1956
Size: 280 1383 293
Fragments arranged by size:
1383 293 280
AfeI AGC'GCT
Cuts at: 0 967 1956
Size: 967 989
AflIII A'CryG_T
Cuts at: 0 929 1385 1682 1956
Size: 929 456 297 274
Fragments arranged by size:
929 456 297 274
AhdI GACnn_n'nnGTC
Cuts at: 0 1212 1956
Size: 1212 744
AleI CACnn'nnGTG
Cuts at: 0 469 1894 1956
Size: 469 1425 62
Fragments arranged by size:
1425 469 62
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 1956
Size: 1742 32 182
Fragments arranged by size:
1742 182 32
AlwNI CAG_nnn'CTG
Cuts at: 0 838 891 1332 1956
Size: 838 53 441 624
Fragments arranged by size:
838 624 441 53
ApaLI G'TGCA_C
Cuts at: 0 1416 1956
Size: 1416 540
ApoI r'AATT_y
Cuts at: 0 1918 1956
Size: 1918 38
AvaI C'yCGr_G
Cuts at: 0 10 440 951 1303 1956
Size: 10 430 511 352 653
Fragments arranged by size:
653 511 430 352 10
BanII G_rGCy'C
Cuts at: 0 728 1323 1580 1956
Size: 728 595 257 376
Fragments arranged by size:
728 595 376 257
BbvCI CC'TCA_GC
Cuts at: 0 657 1484 1956
Size: 657 827 472
Fragments arranged by size:
827 657 472
BfrBI ATG'CAT
Cuts at: 0 875 1956
Size: 875 1081
BglI GCCn_nnn'nGGC
Cuts at: 0 1327 1956
Size: 1327 629
Bme1580I G_kGCm'C
Cuts at: 0 1393 1420 1956
Size: 1393 27 536
Fragments arranged by size:
1393 536 27
BmgBI CAC'GTC
Cuts at: 0 90 1352 1956
Size: 90 1262 604
Fragments arranged by size:
1262 604 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 1536 1881 1956
Size: 305 1231 345 75
Fragments arranged by size:
1231 345 305 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 1956
Size: 588 1368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 558 630 1210 1693 1956
Size: 191 367 72 580 483 263
Fragments arranged by size:
580 483 367 263 191 72
Bpu10I CC'TnA_GC
Cuts at: 0 657 1484 1956
Size: 657 827 472
Fragments arranged by size:
827 657 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1106 1956
Size: 1106 850
BsaI GGTCTCn'nnnn_
Cuts at: 0 171 1956
Size: 171 1785
BsaAI yAC'GTr
Cuts at: 0 1386 1956
Size: 1386 570
BsaHI Gr'CG_yC
Cuts at: 0 505 824 1956
Size: 505 319 1132
Fragments arranged by size:
1132 505 319
BsaWI w'CCGG_w
Cuts at: 0 333 1445 1956
Size: 333 1112 511
Fragments arranged by size:
1112 511 333
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 117 147 955 985 1742 1772 1956
Size: 117 30 808 30 757 30 184
Fragments arranged by size:
808 757 184 117 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 761 874 1956
Size: 88 673 113 1082
Fragments arranged by size:
1082 673 113 88
BseYI C'CCAG_C
Cuts at: 0 151 838 1296 1956
Size: 151 687 458 660
Fragments arranged by size:
687 660 458 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 440 880 1518 1956
Size: 434 6 440 638 438
Fragments arranged by size:
638 440 438 434 6
BsiEI CG_ry'CG
Cuts at: 0 1446 1956
Size: 1446 510
BsiHKAI G_wGCw'C
Cuts at: 0 1420 1580 1956
Size: 1420 160 376
Fragments arranged by size:
1420 376 160
BsmBI CGTCTCn'nnnn_
Cuts at: 0 120 856 1956
Size: 120 736 1100
Fragments arranged by size:
1100 736 120
Bsp1286I G_dGCh'C
Cuts at: 0 728 1323 1393 1420 1580 1956
Size: 728 595 70 27 160 376
Fragments arranged by size:
728 595 376 160 70 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 406 899 994 1193 1429 1956
Size: 406 493 95 199 236 527
Fragments arranged by size:
527 493 406 236 199 95
BsrBI CCG'CTC
Cuts at: 0 6 1005 1956
Size: 6 999 951
Fragments arranged by size:
999 951 6
BsrFI r'CCGG_y
Cuts at: 0 1247 1543 1956
Size: 1247 296 413
Fragments arranged by size:
1247 413 296
BsrGI T'GTAC_A
Cuts at: 0 317 1956
Size: 317 1639
BssSI C'ACGA_G
Cuts at: 0 817 1036 1699 1956
Size: 817 219 663 257
Fragments arranged by size:
817 663 257 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 881 1424 1956
Size: 881 543 532
Fragments arranged by size:
881 543 532
BstYI r'GATC_y
Cuts at: 0 1007 1956
Size: 1007 949
Bsu36I CC'TnA_GG
Cuts at: 0 97 408 1956
Size: 97 311 1548
Fragments arranged by size:
1548 311 97
BtgI C'CryG_G
Cuts at: 0 539 998 1956
Size: 539 459 958
Fragments arranged by size:
958 539 459
BtsI GCAGTG_nn'
Cuts at: 0 1083 1258 1290 1495 1956
Size: 1083 175 32 205 461
Fragments arranged by size:
1083 461 205 175 32
DraIII CAC_nnn'GTG
Cuts at: 0 345 1956
Size: 345 1611
DrdI GACnn_nn'nnGTC
Cuts at: 0 1474 1956
Size: 1474 482
EaeI y'GGCC_r
Cuts at: 0 1844 1956
Size: 1844 112
EarI CTCTTCn'nnn_
Cuts at: 0 520 540 1956
Size: 520 20 1416
Fragments arranged by size:
1416 520 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 138 1956
Size: 138 1818
EcoICRI GAG'CTC
Cuts at: 0 1578 1956
Size: 1578 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 280 558 630 1210 1663 1693 1956
Size: 191 89 278 72 580 453 30 263
Fragments arranged by size:
580 453 278 263 191 89 72 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1397 1956
Size: 1397 559
EcoO109I rG'GnC_Cy
Cuts at: 0 444 756 888 1182 1329 1467 1956
Size: 444 312 132 294 147 138 489
Fragments arranged by size:
489 444 312 294 147 138 132
FspI TGC'GCA
Cuts at: 0 1783 1956
Size: 1783 173
HaeII r_GCGC'y
Cuts at: 0 969 1956
Size: 969 987
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 628 660 955 987 1198 1230
Size: 187 32 409 32 295 32 211 32
Cuts at: 1230 1330 1362 1956
Size: 100 32 594
Fragments arranged by size:
594 409 295 211 187 100 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1206 1688 1956
Size: 1206 482 268
Fragments arranged by size:
1206 482 268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1956
Size: 677 1279
MslI CAynn'nnrTG
Cuts at: 0 469 1894 1931 1956
Size: 469 1425 37 25
Fragments arranged by size:
1425 469 37 25
MspA1I CmG'CkG
Cuts at: 0 835 992 1713 1956
Size: 835 157 721 243
Fragments arranged by size:
835 721 243 157
NaeI GCC'GGC
Cuts at: 0 1249 1956
Size: 1249 707
NgoMIV G'CCGG_C
Cuts at: 0 1247 1956
Size: 1247 709
NsiI A_TGCA'T
Cuts at: 0 877 1956
Size: 877 1079
NspI r_CATG'y
Cuts at: 0 284 875 927 1131 1686 1956
Size: 284 591 52 204 555 270
Fragments arranged by size:
591 555 284 270 204 52
PasI CC'CwG_GG
Cuts at: 0 44 1332 1956
Size: 44 1288 624
Fragments arranged by size:
1288 624 44
PciI A'CATG_T
Cuts at: 0 1682 1956
Size: 1682 274
PfoI T'CCnGG_A
Cuts at: 0 1376 1956
Size: 1376 580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1742 1774 1956
Size: 1742 32 182
Fragments arranged by size:
1742 182 32
PpuMI rG'GwC_Cy
Cuts at: 0 444 756 888 1182 1467 1956
Size: 444 312 132 294 285 489
Fragments arranged by size:
489 444 312 294 285 132
PshAI GACnn'nnGTC
Cuts at: 0 1070 1372 1595 1956
Size: 1070 302 223 361
Fragments arranged by size:
1070 361 302 223
PstI C_TGCA'G
Cuts at: 0 558 1136 1508 1956
Size: 558 578 372 448
Fragments arranged by size:
578 558 448 372
RsrII CG'GwC_CG
Cuts at: 0 1541 1956
Size: 1541 415
SacI G_AGCT'C
Cuts at: 0 1580 1956
Size: 1580 376
SanDI GG'GwC_CC
Cuts at: 0 444 1956
Size: 444 1512
SbfI CC_TGCA'GG
Cuts at: 0 558 1956
Size: 558 1398
SfcI C'TryA_G
Cuts at: 0 180 554 1132 1504 1956
Size: 180 374 578 372 452
Fragments arranged by size:
578 452 374 372 180
SmaI CCC'GGG
Cuts at: 0 442 1956
Size: 442 1514
SmlI C'TyrA_G
Cuts at: 0 1121 1956
Size: 1121 835
SphI G_CATG'C
Cuts at: 0 927 1956
Size: 927 1029
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1576 1705 1956
Size: 1576 129 251
Fragments arranged by size:
1576 251 129
TatI w'GTAC_w
Cuts at: 0 317 1262 1523 1956
Size: 317 945 261 433
Fragments arranged by size:
945 433 317 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1372 1404 1956
Size: 1372 32 552
Fragments arranged by size:
1372 552 32
Tth111I GACn'n_nGTC
Cuts at: 0 681 1956
Size: 681 1275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 159 1956
Size: 159 1797
XmaI C'CCGG_G
Cuts at: 0 440 1956
Size: 440 1516
ZraI GAC'GTC
Cuts at: 0 506 825 1956
Size: 506 319 1131
Fragments arranged by size:
1131 506 319
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaLI ApoI AvaI BanII BbvCI BfrBI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NsiI
NspI PasI PciI PfoI PpiI PpuMI PshAI PstI
RsrII SacI SanDI SbfI SfcI SmaI SmlI SphI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AlfI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BglII BlpI BmtI BsaBI BsiWI
BsmI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI EagI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NheI NotI NruI
PacI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI PvuII SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI XbaI XhoI XmnI