Restriction map for
pFN21AB9939
(Linear) (Six-base) MAPSORT of: KIBB9939.seq Check: 9707 from: 1 to: 1581
KIBB9939.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
Acc65I G'GTAC_C
Cuts at: 0 1176 1581
Size: 1176 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1581
Size: 82 1499
AlwNI CAG_nnn'CTG
Cuts at: 0 1277 1581
Size: 1277 304
ApoI r'AATT_y
Cuts at: 0 313 351 484 580 1162 1211 1581
Size: 313 38 133 96 582 49 370
Fragments arranged by size:
582 370 313 133 96 49 38
AseI AT'TA_AT
Cuts at: 0 146 773 1581
Size: 146 627 808
Fragments arranged by size:
808 627 146
BamHI G'GATC_C
Cuts at: 0 1104 1581
Size: 1104 477
BanI G'GyrC_C
Cuts at: 0 3 1176 1581
Size: 3 1173 405
Fragments arranged by size:
1173 405 3
BanII G_rGCy'C
Cuts at: 0 740 1581
Size: 740 841
BbeI G_GCGC'C
Cuts at: 0 7 1581
Size: 7 1574
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 1233 1256 1581
Size: 814 419 23 325
Fragments arranged by size:
814 419 325 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 1424 1581
Size: 1424 157
BglI GCCn_nnn'nGGC
Cuts at: 0 12 1581
Size: 12 1569
Bme1580I G_kGCm'C
Cuts at: 0 1424 1581
Size: 1424 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 1372 1581
Size: 1372 209
BsaHI Gr'CG_yC
Cuts at: 0 4 1581
Size: 4 1577
BsaWI w'CCGG_w
Cuts at: 0 447 1581
Size: 447 1134
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 434 853 1581
Size: 434 419 728
Fragments arranged by size:
728 434 419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 965 1290 1581
Size: 329 636 325 291
Fragments arranged by size:
636 329 325 291
BsiHKAI G_wGCw'C
Cuts at: 0 251 1581
Size: 251 1330
BsmI GAATG_Cn'
Cuts at: 0 223 1483 1581
Size: 223 1260 98
Fragments arranged by size:
1260 223 98
Bsp1286I G_dGCh'C
Cuts at: 0 251 740 1424 1581
Size: 251 489 684 157
Fragments arranged by size:
684 489 251 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 958 1581
Size: 958 623
BsrBI CCG'CTC
Cuts at: 0 12 1581
Size: 12 1569
BsrDI GCAATG_nn'
Cuts at: 0 1436 1581
Size: 1436 145
BsrGI T'GTAC_A
Cuts at: 0 293 1222 1581
Size: 293 929 359
Fragments arranged by size:
929 359 293
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1277 1581
Size: 1277 304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1385 1581
Size: 1385 196
BstYI r'GATC_y
Cuts at: 0 594 1104 1581
Size: 594 510 477
Fragments arranged by size:
594 510 477
BtgI C'CryG_G
Cuts at: 0 63 1581
Size: 63 1518
EarI CTCTTCn'nnn_
Cuts at: 0 101 830 1581
Size: 101 729 751
Fragments arranged by size:
751 729 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 1581
Size: 24 1557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1581
Size: 82 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 419 787 1581
Size: 419 368 794
Fragments arranged by size:
794 419 368
EcoRI G'AATT_C
Cuts at: 0 1162 1211 1581
Size: 1162 49 370
Fragments arranged by size:
1162 370 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 1350 1581
Size: 183 1167 231
Fragments arranged by size:
1167 231 183
HaeII r_GCGC'y
Cuts at: 0 7 31 1581
Size: 7 24 1550
Fragments arranged by size:
1550 24 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 444 476 1398 1430 1581
Size: 444 32 922 32 151
Fragments arranged by size:
922 444 151 32 32
HindIII A'AGCT_T
Cuts at: 0 1007 1581
Size: 1007 574
KasI G'GCGC_C
Cuts at: 0 3 1581
Size: 3 1578
KpnI G_GTAC'C
Cuts at: 0 1180 1581
Size: 1180 401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 863 970 1581
Size: 636 227 107 611
Fragments arranged by size:
636 611 227 107
MslI CAynn'nnrTG
Cuts at: 0 428 467 710 1581
Size: 428 39 243 871
Fragments arranged by size:
871 428 243 39
NarI GG'CG_CC
Cuts at: 0 4 1581
Size: 4 1577
NdeI CA'TA_TG
Cuts at: 0 987 1392 1581
Size: 987 405 189
Fragments arranged by size:
987 405 189
NspI r_CATG'y
Cuts at: 0 665 1581
Size: 665 916
PflMI CCAn_nnn'nTGG
Cuts at: 0 1513 1581
Size: 1513 68
PfoI T'CCnGG_A
Cuts at: 0 212 1107 1581
Size: 212 895 474
Fragments arranged by size:
895 474 212
PpuMI rG'GwC_Cy
Cuts at: 0 419 787 1581
Size: 419 368 794
Fragments arranged by size:
794 419 368
SapI GCTCTTCn'nnn_
Cuts at: 0 101 1581
Size: 101 1480
ScaI AGT'ACT
Cuts at: 0 747 1219 1581
Size: 747 472 362
Fragments arranged by size:
747 472 362
SexAI A'CCwGG_T
Cuts at: 0 887 1092 1581
Size: 887 205 489
Fragments arranged by size:
887 489 205
SfcI C'TryA_G
Cuts at: 0 242 1581
Size: 242 1339
SfoI GGC'GCC
Cuts at: 0 5 1581
Size: 5 1576
SphI G_CATG'C
Cuts at: 0 665 1581
Size: 665 916
SspI AAT'ATT
Cuts at: 0 771 1581
Size: 771 810
StyI C'CwwG_G
Cuts at: 0 1129 1341 1581
Size: 1129 212 240
Fragments arranged by size:
1129 240 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 744 1581
Size: 744 837
TatI w'GTAC_w
Cuts at: 0 293 520 745 1217 1222 1581
Size: 293 227 225 472 5 359
Fragments arranged by size:
472 359 293 227 225 5
XmnI GAAnn'nnTTC
Cuts at: 0 95 908 1581
Size: 95 813 673
Fragments arranged by size:
813 673 95
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AseI BamHI BanI BanII
BbeI BbsI BciVI BglI Bme1580I BmrI BsaHI BsaWI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrGI BstAPI BstXI BstYI BtgI EarI EciI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI KpnI
MmeI MslI NarI NdeI NspI PflMI PfoI PpuMI
SapI ScaI SexAI SfcI SfoI SphI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbvCI BcgI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HincII
HpaI MfeI MluI MscI MspA1I NaeI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI