Restriction map for
pFN21AB9901
(Linear) (Six-base) MAPSORT of: KIBB9901.seq Check: 8749 from: 1 to: 768
KIBB9901.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AatII G_ACGT'C
Cuts at: 0 263 768
Size: 263 505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 439 768
Size: 109 330 329
Fragments arranged by size:
330 329 109
AleI CACnn'nnGTG
Cuts at: 0 746 768
Size: 746 22
AlwNI CAG_nnn'CTG
Cuts at: 0 28 84 306 408 483 492 768
Size: 28 56 222 102 75 9 276
Fragments arranged by size:
276 222 102 75 56 28 9
ApaI G_GGCC'C
Cuts at: 0 604 768
Size: 604 164
BanII G_rGCy'C
Cuts at: 0 572 604 768
Size: 572 32 164
Fragments arranged by size:
572 164 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 768
Size: 290 478
BbvCI CC'TCA_GC
Cuts at: 0 181 544 768
Size: 181 363 224
Fragments arranged by size:
363 224 181
Bme1580I G_kGCm'C
Cuts at: 0 604 768
Size: 604 164
BmgBI CAC'GTC
Cuts at: 0 194 768
Size: 194 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 264 488 768
Size: 114 150 224 280
Fragments arranged by size:
280 224 150 114
Bpu10I CC'TnA_GC
Cuts at: 0 181 544 566 768
Size: 181 363 22 202
Fragments arranged by size:
363 202 181 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 315 768
Size: 315 453
BsaHI Gr'CG_yC
Cuts at: 0 260 768
Size: 260 508
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 486 516 554 584 768
Size: 295 30 161 30 38 30 184
Fragments arranged by size:
295 184 161 38 30 30 30
BseYI C'CCAG_C
Cuts at: 0 21 199 768
Size: 21 178 569
Fragments arranged by size:
569 178 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 768
Size: 504 264
BsiHKAI G_wGCw'C
Cuts at: 0 572 768
Size: 572 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 200 768
Size: 200 568
Bsp1286I G_dGCh'C
Cuts at: 0 572 604 768
Size: 572 32 164
Fragments arranged by size:
572 164 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 768
Size: 84 684
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 149 768
Size: 149 619
BtsI GCAGTG_nn'
Cuts at: 0 329 420 768
Size: 329 91 348
Fragments arranged by size:
348 329 91
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 768
Size: 259 509
EaeI y'GGCC_r
Cuts at: 0 293 768
Size: 293 475
EarI CTCTTCn'nnn_
Cuts at: 0 97 768
Size: 97 671
EcoICRI GAG'CTC
Cuts at: 0 570 768
Size: 570 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 114 264 439 488 768
Size: 109 5 150 175 49 280
Fragments arranged by size:
280 175 150 109 49 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 768
Size: 496 272
EcoO109I rG'GnC_Cy
Cuts at: 0 303 459 489 600 601 683 728 768
Size: 303 156 30 111 1 82 45 40
Fragments arranged by size:
303 156 111 82 45 40 30 1
HaeII r_GCGC'y
Cuts at: 0 383 768
Size: 383 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 402 434 486 518 768
Size: 295 32 75 32 52 32 250
Fragments arranged by size:
295 250 75 52 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 221 746 768
Size: 221 525 22
Fragments arranged by size:
525 221 22
NspI r_CATG'y
Cuts at: 0 220 768
Size: 220 548
PflMI CCAn_nnn'nTGG
Cuts at: 0 28 306 768
Size: 28 278 462
Fragments arranged by size:
462 278 28
PfoI T'CCnGG_A
Cuts at: 0 359 768
Size: 359 409
PpuMI rG'GwC_Cy
Cuts at: 0 303 459 683 768
Size: 303 156 224 85
Fragments arranged by size:
303 224 156 85
PspOMI G'GGCC_C
Cuts at: 0 600 768
Size: 600 168
PstI C_TGCA'G
Cuts at: 0 488 653 768
Size: 488 165 115
Fragments arranged by size:
488 165 115
SacI G_AGCT'C
Cuts at: 0 572 768
Size: 572 196
SanDI GG'GwC_CC
Cuts at: 0 683 768
Size: 683 85
SexAI A'CCwGG_T
Cuts at: 0 287 305 768
Size: 287 18 463
Fragments arranged by size:
463 287 18
SfcI C'TryA_G
Cuts at: 0 383 484 649 768
Size: 383 101 165 119
Fragments arranged by size:
383 165 119 101
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 511 768
Size: 225 286 257
Fragments arranged by size:
286 257 225
TatI w'GTAC_w
Cuts at: 0 581 768
Size: 581 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 451 483 554 586 768
Size: 451 32 71 32 182
Fragments arranged by size:
451 182 71 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 307 768
Size: 307 461
ZraI GAC'GTC
Cuts at: 0 261 768
Size: 261 507
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI BanII BbsI BbvCI
Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BsaXI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BstAPI BtgZI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
MslI NspI PflMI PfoI PpuMI PspOMI PstI SacI
SanDI SexAI SfcI TaqII TatI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI XbaI
XcmI XhoI XmaI XmnI