Restriction map for
pFN21AB9896
(Linear) (Six-base) MAPSORT of: KIBB9896.seq Check: 7275 from: 1 to: 3531
KIBB9896.orf
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2000 2807 3531
Size: 2000 807 724
Fragments arranged by size:
2000 807 724
AccI GT'mk_AC
Cuts at: 0 1652 1787 2105 2696 3531
Size: 1652 135 318 591 835
Fragments arranged by size:
1652 835 591 318 135
Acc65I G'GTAC_C
Cuts at: 0 2603 2939 3531
Size: 2603 336 592
Fragments arranged by size:
2603 592 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1300 3531
Size: 172 1128 2231
Fragments arranged by size:
2231 1128 172
AfeI AGC'GCT
Cuts at: 0 1090 3531
Size: 1090 2441
AleI CACnn'nnGTG
Cuts at: 0 571 913 1321 3531
Size: 571 342 408 2210
Fragments arranged by size:
2210 571 408 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 931 2072 2177 3531
Size: 931 1141 105 1354
Fragments arranged by size:
1354 1141 931 105
AlwNI CAG_nnn'CTG
Cuts at: 0 42 271 507 642 729 760 1719 1902
Size: 42 229 236 135 87 31 959 183
Cuts at: 1902 2616 2899 3531
Size: 714 283 632
Fragments arranged by size:
959 714 632 283 236 229 183 135
87 42 31
ApaI G_GGCC'C
Cuts at: 0 519 1617 1713 2874 3531
Size: 519 1098 96 1161 657
Fragments arranged by size:
1161 1098 657 519 96
ApaLI G'TGCA_C
Cuts at: 0 1560 3531
Size: 1560 1971
ApoI r'AATT_y
Cuts at: 0 2584 3531
Size: 2584 947
AvaI C'yCGr_G
Cuts at: 0 622 1616 1871 2087 2599 2804 2867 3403
Size: 622 994 255 216 512 205 63 536
Cuts at: 3403 3445 3531
Size: 42 86
Fragments arranged by size:
994 622 536 512 255 216 205 86
63 42
BanI G'GyrC_C
Cuts at: 0 907 1681 2153 2603 2939 3085 3399 3531
Size: 907 774 472 450 336 146 314 132
Fragments arranged by size:
907 774 472 450 336 314 146 132
BanII G_rGCy'C
Cuts at: 0 331 519 1127 1617 1713 2646 2874 3531
Size: 331 188 608 490 96 933 228 657
Fragments arranged by size:
933 657 608 490 331 228 188 96
BbeI G_GCGC'C
Cuts at: 0 3403 3531
Size: 3403 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 862 1348 1563 2275 3531
Size: 185 677 486 215 712 1256
Fragments arranged by size:
1256 712 677 486 215 185
BbvCI CC'TCA_GC
Cuts at: 0 3428 3531
Size: 3428 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1170 1204 3531
Size: 1170 34 2327
Fragments arranged by size:
2327 1170 34
BglI GCCn_nnn'nGGC
Cuts at: 0 139 751 1918 2329 2338 3293 3336 3531
Size: 139 612 1167 411 9 955 43 195
Fragments arranged by size:
1167 955 612 411 195 139 43 9
BglII A'GATC_T
Cuts at: 0 2240 3531
Size: 2240 1291
BlpI GC'TnA_GC
Cuts at: 0 2771 3531
Size: 2771 760
Bme1580I G_kGCm'C
Cuts at: 0 265 519 1147 1564 1617 1684 1713 1923
Size: 265 254 628 417 53 67 29 210
Cuts at: 1923 2156 2187 2325 2785 2874 3088 3531
Size: 233 31 138 460 89 214 443
Fragments arranged by size:
628 460 443 417 265 254 233 214
210 138 89 67 53 31 29
BmgBI CAC'GTC
Cuts at: 0 1323 3531
Size: 1323 2208
BmrI ACTGGGnnnn_n'
Cuts at: 0 1468 1707 1723 1933 2464 3531
Size: 1468 239 16 210 531 1067
Fragments arranged by size:
1468 1067 531 239 210 16
BmtI G_CTAG'C
Cuts at: 0 2078 3531
Size: 2078 1453
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 350 3531
Size: 350 3181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1857 3340 3531
Size: 1857 1483 191
Fragments arranged by size:
1857 1483 191
Bpu10I CC'TnA_GC
Cuts at: 0 349 2314 3142 3428 3531
Size: 349 1965 828 286 103
Fragments arranged by size:
1965 828 349 286 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1213 3136 3531
Size: 1111 102 1923 395
Fragments arranged by size:
1923 1111 395 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 892 906 2820 3531
Size: 892 14 1914 711
Fragments arranged by size:
1914 892 711 14
BsaHI Gr'CG_yC
Cuts at: 0 131 3400 3531
Size: 131 3269 131
Fragments arranged by size:
3269 131 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 857 887 1084 1114 2434 2464 2644 2674
Size: 857 30 197 30 1320 30 180 30
Cuts at: 2674 3239 3269 3293 3323 3531
Size: 565 30 24 30 208
Fragments arranged by size:
1320 857 565 208 197 180 30 30
30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 321 1089 1960 2776 3247 3531
Size: 321 768 871 816 471 284
Fragments arranged by size:
871 816 768 471 321 284
BseYI C'CCAG_C
Cuts at: 0 42 244 389 537 543 586 632 719
Size: 42 202 145 148 6 43 46 87
Cuts at: 719 1608 1627 1677 1706 1895 2543 2554 2877
Size: 889 19 50 29 189 648 11 323
Cuts at: 2877 3072 3531
Size: 195 459
Fragments arranged by size:
889 648 459 323 202 195 189 148
145 87 50 46 43 42 29 19
11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 937 1504 2101 2110 2225 2833 3016
Size: 380 557 567 597 9 115 608 183
Cuts at: 3016 3457 3531
Size: 441 74
Fragments arranged by size:
608 597 567 557 441 380 183 115
74 9
BsiEI CG_ry'CG
Cuts at: 0 1513 3264 3462 3531
Size: 1513 1751 198 69
Fragments arranged by size:
1751 1513 198 69
BsiHKAI G_wGCw'C
Cuts at: 0 331 1127 1328 1564 1763 2061 2429 2529
Size: 331 796 201 236 199 298 368 100
Cuts at: 2529 2798 3347 3531
Size: 269 549 184
Fragments arranged by size:
796 549 368 331 298 269 236 201
199 184 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 690 1520 1770 2008 2084 3531
Size: 690 830 250 238 76 1447
Fragments arranged by size:
1447 830 690 250 238 76
Bsp1286I G_dGCh'C
Cuts at: 0 265 331 519 1127 1147 1328 1564 1617
Size: 265 66 188 608 20 181 236 53
Cuts at: 1617 1684 1713 1763 1923 2061 2156 2187 2325
Size: 67 29 50 160 138 95 31 138
Cuts at: 2325 2429 2529 2646 2785 2798 2874 3088 3347
Size: 104 100 117 139 13 76 214 259
Cuts at: 3347 3531
Size: 184
Fragments arranged by size:
608 265 259 236 214 188 184 181
160 139 138 138 117 104 100 95
76 67 66 53 50 31 29 20
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 874 2000 2615 2807 2951 3023 3386
Size: 783 91 1126 615 192 144 72 363
Cuts at: 3386 3531
Size: 145
Fragments arranged by size:
1126 783 615 363 192 145 144 91
72
BsrBI CCG'CTC
Cuts at: 0 353 1097 1544 2775 3531
Size: 353 744 447 1231 756
Fragments arranged by size:
1231 756 744 447 353
BsrFI r'CCGG_y
Cuts at: 0 1007 1928 2689 3531
Size: 1007 921 761 842
Fragments arranged by size:
1007 921 842 761
BsrGI T'GTAC_A
Cuts at: 0 2450 3531
Size: 2450 1081
BssHII G'CGCG_C
Cuts at: 0 3436 3531
Size: 3436 95
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 2124 2203 2212 3531
Size: 642 1482 79 9 1319
Fragments arranged by size:
1482 1319 642 79 9
BstBI TT'CG_AA
Cuts at: 0 176 3531
Size: 176 3355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 1051 1663 1736 2172 3531
Size: 126 925 612 73 436 1359
Fragments arranged by size:
1359 925 612 436 126 73
BstYI r'GATC_y
Cuts at: 0 785 1131 1284 2240 3531
Size: 785 346 153 956 1291
Fragments arranged by size:
1291 956 785 346 153
Bsu36I CC'TnA_GG
Cuts at: 0 3151 3531
Size: 3151 380
BtgI C'CryG_G
Cuts at: 0 161 749 1050 2132 2281 2387 2437 3209
Size: 161 588 301 1082 149 106 50 772
Cuts at: 3209 3325 3531
Size: 116 206
Fragments arranged by size:
1082 772 588 301 206 161 149 116
106 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1828 3531
Size: 1828 1703
BtsI GCAGTG_nn'
Cuts at: 0 1253 1311 1437 1452 1458 2159 2325 2377
Size: 1253 58 126 15 6 701 166 52
Cuts at: 2377 3531
Size: 1154
Fragments arranged by size:
1253 1154 701 166 126 58 52 15
6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2270 2305 3531
Size: 2270 35 1226
Fragments arranged by size:
2270 1226 35
DraIII CAC_nnn'GTG
Cuts at: 0 2347 2388 3531
Size: 2347 41 1143
Fragments arranged by size:
2347 1143 41
DrdI GACnn_nn'nnGTC
Cuts at: 0 1474 3531
Size: 1474 2057
EaeI y'GGCC_r
Cuts at: 0 1510 2234 2459 2678 2705 3531
Size: 1510 724 225 219 27 826
Fragments arranged by size:
1510 826 724 225 219 27
EagI C'GGCC_G
Cuts at: 0 1510 3531
Size: 1510 2021
EarI CTCTTCn'nnn_
Cuts at: 0 455 3531
Size: 455 3076
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 965 3099 3328 3531
Size: 965 2134 229 203
Fragments arranged by size:
2134 965 229 203
EcoICRI GAG'CTC
Cuts at: 0 329 1125 3531
Size: 329 796 2406
Fragments arranged by size:
2406 796 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1300 1857 3340 3531
Size: 172 1128 557 1483 191
Fragments arranged by size:
1483 1128 557 191 172
EcoNI CCTnn'n_nnAGG
Cuts at: 0 575 596 2047 2485 3202 3531
Size: 575 21 1451 438 717 329
Fragments arranged by size:
1451 717 575 438 329 21
EcoO109I rG'GnC_Cy
Cuts at: 0 515 516 675 795 925 1029 1613 1819
Size: 515 1 159 120 130 104 584 206
Cuts at: 1819 2040 2871 3098 3138 3286 3531
Size: 221 831 227 40 148 245
Fragments arranged by size:
831 584 515 245 227 221 206 159
148 130 120 104 40 1
EcoRI G'AATT_C
Cuts at: 0 2584 3531
Size: 2584 947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 202 3531
Size: 202 3329
FseI GG_CCGG'CC
Cuts at: 0 1932 3531
Size: 1932 1599
FspI TGC'GCA
Cuts at: 0 1180 1252 3031 3531
Size: 1180 72 1779 500
Fragments arranged by size:
1779 1180 500 72
FspAI rTGC'GCAy
Cuts at: 0 3031 3531
Size: 3031 500
HaeII r_GCGC'y
Cuts at: 0 43 73 370 1092 2081 2087 2179 3403
Size: 43 30 297 722 989 6 92 1224
Cuts at: 3403 3531
Size: 128
Fragments arranged by size:
1224 989 722 297 128 92 43 30
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 1276 1308 1372 1404 2203 2235
Size: 274 32 970 32 64 32 799 32
Cuts at: 2235 3531
Size: 1296
Fragments arranged by size:
1296 970 799 274 64 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 3451 3531
Size: 3451 80
KasI G'GCGC_C
Cuts at: 0 3399 3531
Size: 3399 132
KpnI G_GTAC'C
Cuts at: 0 2607 2943 3531
Size: 2607 336 588
Fragments arranged by size:
2607 588 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3156 3531
Size: 3156 375
MscI TGG'CCA
Cuts at: 0 2680 2707 3531
Size: 2680 27 824
Fragments arranged by size:
2680 824 27
MslI CAynn'nnrTG
Cuts at: 0 571 913 1321 3531
Size: 571 342 408 2210
Fragments arranged by size:
2210 571 408 342
MspA1I CmG'CkG
Cuts at: 0 642 742 1018 1439 1509 1902 2018 2124
Size: 642 100 276 421 70 393 116 106
Cuts at: 2124 2569 2613 2624 2731 2787 3000 3327 3531
Size: 445 44 11 107 56 213 327 204
Fragments arranged by size:
642 445 421 393 327 276 213 204
116 107 106 100 70 56 44 11
NaeI GCC'GGC
Cuts at: 0 1930 3531
Size: 1930 1601
NarI GG'CG_CC
Cuts at: 0 3400 3531
Size: 3400 131
NcoI C'CATG_G
Cuts at: 0 161 2132 2437 3531
Size: 161 1971 305 1094
Fragments arranged by size:
1971 1094 305 161
NgoMIV G'CCGG_C
Cuts at: 0 1928 3531
Size: 1928 1603
NheI G'CTAG_C
Cuts at: 0 2074 3531
Size: 2074 1457
NspI r_CATG'y
Cuts at: 0 186 1536 3423 3531
Size: 186 1350 1887 108
Fragments arranged by size:
1887 1350 186 108
PasI CC'CwG_GG
Cuts at: 0 442 1663 2560 2837 3081 3531
Size: 442 1221 897 277 244 450
Fragments arranged by size:
1221 897 450 442 277 244
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 2372 2388 2714 3041 3531
Size: 711 1661 16 326 327 490
Fragments arranged by size:
1661 711 490 327 326 16
PfoI T'CCnGG_A
Cuts at: 0 594 2504 3531
Size: 594 1910 1027
Fragments arranged by size:
1910 1027 594
PpuMI rG'GwC_Cy
Cuts at: 0 675 795 3138 3531
Size: 675 120 2343 393
Fragments arranged by size:
2343 675 393 120
PshAI GACnn'nnGTC
Cuts at: 0 3266 3517 3531
Size: 3266 251 14
Fragments arranged by size:
3266 251 14
PspOMI G'GGCC_C
Cuts at: 0 515 1613 1709 2870 3531
Size: 515 1098 96 1161 661
Fragments arranged by size:
1161 1098 661 515 96
PstI C_TGCA'G
Cuts at: 0 488 812 2000 2048 2612 2816 2948 3531
Size: 488 324 1188 48 564 204 132 583
Fragments arranged by size:
1188 583 564 488 324 204 132 48
PvuI CG_AT'CG
Cuts at: 0 3462 3531
Size: 3462 69
PvuII CAG'CTG
Cuts at: 0 742 1018 1902 2124 2569 2613 2624 2731
Size: 742 276 884 222 445 44 11 107
Cuts at: 2731 3000 3531
Size: 269 531
Fragments arranged by size:
884 742 531 445 276 269 222 107
44 11
SacI G_AGCT'C
Cuts at: 0 331 1127 3531
Size: 331 796 2404
Fragments arranged by size:
2404 796 331
SacII CC_GC'GG
Cuts at: 0 3328 3531
Size: 3328 203
SanDI GG'GwC_CC
Cuts at: 0 675 3531
Size: 675 2856
SbfI CC_TGCA'GG
Cuts at: 0 2948 3531
Size: 2948 583
SfcI C'TryA_G
Cuts at: 0 484 608 808 1996 2044 2608 2652 2812
Size: 484 124 200 1188 48 564 44 160
Cuts at: 2812 2944 3531
Size: 132 587
Fragments arranged by size:
1188 587 564 484 200 160 132 124
48 44
SfoI GGC'GCC
Cuts at: 0 3401 3531
Size: 3401 130
SgrAI Cr'CCGG_yG
Cuts at: 0 2689 3531
Size: 2689 842
SmaI CCC'GGG
Cuts at: 0 624 1618 1873 2806 2869 3405 3531
Size: 624 994 255 933 63 536 126
Fragments arranged by size:
994 933 624 536 255 126 63
SmlI C'TyrA_G
Cuts at: 0 1126 1228 3115 3445 3531
Size: 1126 102 1887 330 86
Fragments arranged by size:
1887 1126 330 102 86
SphI G_CATG'C
Cuts at: 0 1536 3423 3531
Size: 1536 1887 108
Fragments arranged by size:
1887 1536 108
SrfI GCCC'GGGC
Cuts at: 0 2869 3405 3531
Size: 2869 536 126
Fragments arranged by size:
2869 536 126
StuI AGG'CCT
Cuts at: 0 2332 3355 3531
Size: 2332 1023 176
Fragments arranged by size:
2332 1023 176
StyI C'CwwG_G
Cuts at: 0 136 161 1416 1595 1769 2132 2437 2888
Size: 136 25 1255 179 174 363 305 451
Cuts at: 2888 3531
Size: 643
Fragments arranged by size:
1255 643 451 363 305 179 174 136
25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 414 646 712 1998 3531
Size: 94 320 232 66 1286 1533
Fragments arranged by size:
1533 1286 320 232 94 66
TatI w'GTAC_w
Cuts at: 0 1829 2450 3531
Size: 1829 621 1081
Fragments arranged by size:
1829 1081 621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 518 550 857 889 2294 2326
Size: 464 32 22 32 307 32 1405 32
Cuts at: 2326 3155 3187 3239 3271 3293 3325 3531
Size: 829 32 52 32 22 32 206
Fragments arranged by size:
1405 829 464 307 206 52 32 32
32 32 32 32 32 22 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1947 3531
Size: 1947 1584
XhoI C'TCGA_G
Cuts at: 0 3445 3531
Size: 3445 86
XmaI C'CCGG_G
Cuts at: 0 622 1616 1871 2804 2867 3403 3531
Size: 622 994 255 933 63 536 128
Fragments arranged by size:
994 933 622 536 255 128 63
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuI PvuII SacI SacII SanDI SbfI
SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BssSI BstEII BstZ17I ClaI DraI EcoRV HincII HpaI
MfeI MluI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI RsrII SalI
SapI ScaI SexAI SfiI SgfI SnaBI SpeI SspI
SwaI Tth111I XbaI XmnI ZraI