Restriction map for
pFN21AB9759
(Linear) (Six-base) MAPSORT of: KIBB9759.seq Check: 839 from: 1 to: 2142
KIBB9759.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 422 2142
Size: 422 1720
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1948 2142
Size: 1948 194
AfeI AGC'GCT
Cuts at: 0 1006 1337 2142
Size: 1006 331 805
Fragments arranged by size:
1006 805 331
AflIII A'CryG_T
Cuts at: 0 599 1253 1640 2142
Size: 599 654 387 502
Fragments arranged by size:
654 599 502 387
AgeI A'CCGG_T
Cuts at: 0 1150 2142
Size: 1150 992
AlwNI CAG_nnn'CTG
Cuts at: 0 42 831 2142
Size: 42 789 1311
Fragments arranged by size:
1311 789 42
ApaI G_GGCC'C
Cuts at: 0 562 1052 1103 1394 2108 2142
Size: 562 490 51 291 714 34
Fragments arranged by size:
714 562 490 291 51 34
AvaI C'yCGr_G
Cuts at: 0 300 555 882 889 1042 1189 1228 1698
Size: 300 255 327 7 153 147 39 470
Cuts at: 1698 1879 2142
Size: 181 263
Fragments arranged by size:
470 327 300 263 255 181 153 147
39 7
AvrII C'CTAG_G
Cuts at: 0 1386 2142
Size: 1386 756
BanI G'GyrC_C
Cuts at: 0 18 322 702 1065 2142
Size: 18 304 380 363 1077
Fragments arranged by size:
1077 380 363 304 18
BanII G_rGCy'C
Cuts at: 0 214 562 688 697 896 1052 1103 1229
Size: 214 348 126 9 199 156 51 126
Cuts at: 1229 1394 1431 1835 2108 2142
Size: 165 37 404 273 34
Fragments arranged by size:
404 348 273 214 199 165 156 126
126 51 37 34 9
BbeI G_GCGC'C
Cuts at: 0 22 326 706 1069 2142
Size: 22 304 380 363 1073
Fragments arranged by size:
1073 380 363 304 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 1528 2142
Size: 1528 614
BbvCI CC'TCA_GC
Cuts at: 0 2050 2142
Size: 2050 92
BclI T'GATC_A
Cuts at: 0 89 2142
Size: 89 2053
BglI GCCn_nnn'nGGC
Cuts at: 0 304 331 692 711 1047 1357 1366 1375
Size: 304 27 361 19 336 310 9 9
Cuts at: 1375 1616 1804 1856 2142
Size: 241 188 52 286
Fragments arranged by size:
361 336 310 304 286 241 188 52
27 19 9 9
BlpI GC'TnA_GC
Cuts at: 0 308 722 1921 2077 2142
Size: 308 414 1199 156 65
Fragments arranged by size:
1199 414 308 156 65
Bme1580I G_kGCm'C
Cuts at: 0 562 1052 1103 1394 2108 2142
Size: 562 490 51 291 714 34
Fragments arranged by size:
714 562 490 291 51 34
BmgBI CAC'GTC
Cuts at: 0 87 2142
Size: 87 2055
BmrI ACTGGGnnnn_n'
Cuts at: 0 511 2142
Size: 511 1631
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 1008 2142
Size: 570 438 1134
Fragments arranged by size:
1134 570 438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 853 2026 2142
Size: 15 838 1173 116
Fragments arranged by size:
1173 838 116 15
Bpu10I CC'TnA_GC
Cuts at: 0 2050 2142
Size: 2050 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 373 1606 2142
Size: 142 231 1233 536
Fragments arranged by size:
1233 536 231 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 1141 2142
Size: 1141 1001
BsaAI yAC'GTr
Cuts at: 0 1254 1308 1320 2142
Size: 1254 54 12 822
Fragments arranged by size:
1254 822 54 12
BsaHI Gr'CG_yC
Cuts at: 0 19 323 703 1066 1841 1886 2142
Size: 19 304 380 363 775 45 256
Fragments arranged by size:
775 380 363 304 256 45 19
BsaWI w'CCGG_w
Cuts at: 0 1150 2142
Size: 1150 992
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 913 1159 1339 1342 2142
Size: 850 63 246 180 3 800
Fragments arranged by size:
850 800 246 180 63 3
BseYI C'CCAG_C
Cuts at: 0 757 975 997 1168 1282 1372 2043 2142
Size: 757 218 22 171 114 90 671 99
Fragments arranged by size:
757 671 218 171 114 99 90 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1969 2142
Size: 121 1848 173
Fragments arranged by size:
1848 173 121
BsiEI CG_ry'CG
Cuts at: 0 218 255 287 293 463 620 715 903
Size: 218 37 32 6 170 157 95 188
Cuts at: 903 1034 1361 1799 1808 1913 1949 1955 2039
Size: 131 327 438 9 105 36 6 84
Cuts at: 2039 2069 2102 2115 2127 2142
Size: 30 33 13 12 15
Fragments arranged by size:
438 327 218 188 170 157 131 105
95 84 37 36 33 32 30 15
13 12 9 6 6
BsiHKAI G_wGCw'C
Cuts at: 0 1685 2142
Size: 1685 457
BsmI GAATG_Cn'
Cuts at: 0 1259 2142
Size: 1259 883
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1041 2142
Size: 1041 1101
Bsp1286I G_dGCh'C
Cuts at: 0 214 562 688 697 896 1052 1103 1229
Size: 214 348 126 9 199 156 51 126
Cuts at: 1229 1394 1431 1685 1835 2108 2142
Size: 165 37 254 150 273 34
Fragments arranged by size:
348 273 254 214 199 165 156 150
126 126 51 37 34 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 356 422 2142
Size: 191 165 66 1720
Fragments arranged by size:
1720 191 165 66
BsrBI CCG'CTC
Cuts at: 0 711 1056 2142
Size: 711 345 1086
Fragments arranged by size:
1086 711 345
BsrFI r'CCGG_y
Cuts at: 0 250 489 763 1150 1381 1798 2142
Size: 250 239 274 387 231 417 344
Fragments arranged by size:
417 387 344 274 250 239 231
BssHII G'CGCG_C
Cuts at: 0 119 2142
Size: 119 2023
BssSI C'ACGA_G
Cuts at: 0 364 943 1516 2142
Size: 364 579 573 626
Fragments arranged by size:
626 579 573 364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 407 631 1265 1559 1983 2142
Size: 42 365 224 634 294 424 159
Fragments arranged by size:
634 424 365 294 224 159 42
BstYI r'GATC_y
Cuts at: 0 353 2142
Size: 353 1789
BtgI C'CryG_G
Cuts at: 0 287 293 396 542 569 698 1784 1949
Size: 287 6 103 146 27 129 1086 165
Cuts at: 1949 2142
Size: 193
Fragments arranged by size:
1086 287 193 165 146 129 103 27
6
BtsI GCAGTG_nn'
Cuts at: 0 582 2142
Size: 582 1560
DraIII CAC_nnn'GTG
Cuts at: 0 631 1785 2142
Size: 631 1154 357
Fragments arranged by size:
1154 631 357
DrdI GACnn_nn'nnGTC
Cuts at: 0 1033 2142
Size: 1033 1109
EaeI y'GGCC_r
Cuts at: 0 24 140 215 252 284 290 332 460
Size: 24 116 75 37 32 6 42 128
Cuts at: 460 617 712 900 1031 1182 1358 1367 1499
Size: 157 95 188 131 151 176 9 132
Cuts at: 1499 1796 1805 1910 1946 1952 2024 2036 2066
Size: 297 9 105 36 6 72 12 30
Cuts at: 2066 2099 2112 2142
Size: 33 13 30
Fragments arranged by size:
297 188 176 157 151 132 131 128
116 105 95 75 72 42 37 36
33 32 30 30 24 13 12 9
9 6 6
EagI C'GGCC_G
Cuts at: 0 215 252 284 290 460 617 712 900
Size: 215 37 32 6 170 157 95 188
Cuts at: 900 1031 1358 1796 1805 1910 1946 1952 2036
Size: 131 327 438 9 105 36 6 84
Cuts at: 2036 2066 2099 2112 2142
Size: 30 33 13 30
Fragments arranged by size:
438 327 215 188 170 157 131 105
95 84 37 36 33 32 30 30
13 9 6 6
EarI CTCTTCn'nnn_
Cuts at: 0 118 676 1007 2142
Size: 118 558 331 1135
Fragments arranged by size:
1135 558 331 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 518 2142
Size: 214 304 1624
Fragments arranged by size:
1624 304 214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 853 1948 2026 2142
Size: 15 838 1095 78 116
Fragments arranged by size:
1095 838 116 78 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1400 2142
Size: 1400 742
EcoO109I rG'GnC_Cy
Cuts at: 0 471 607 886 1048 1049 1164 1390 1391
Size: 471 136 279 162 1 115 226 1
Cuts at: 1391 1494 2142
Size: 103 648
Fragments arranged by size:
648 471 279 226 162 136 115 103
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1890 2142
Size: 93 1797 252
Fragments arranged by size:
1797 252 93
FspI TGC'GCA
Cuts at: 0 1606 1690 2142
Size: 1606 84 452
Fragments arranged by size:
1606 452 84
FspAI rTGC'GCAy
Cuts at: 0 1690 2142
Size: 1690 452
HaeII r_GCGC'y
Cuts at: 0 22 152 326 373 396 645 706 764
Size: 22 130 174 47 23 249 61 58
Cuts at: 764 1008 1069 1339 1816 2005 2090 2142
Size: 244 61 270 477 189 85 52
Fragments arranged by size:
477 270 249 244 189 174 130 85
61 61 58 52 47 23 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2116 2142
Size: 2116 26
HincII GTy'rAC
Cuts at: 0 576 2142
Size: 576 1566
KasI G'GCGC_C
Cuts at: 0 18 322 702 1065 2142
Size: 18 304 380 363 1077
Fragments arranged by size:
1077 380 363 304 18
MluI A'CGCG_T
Cuts at: 0 599 1640 2142
Size: 599 1041 502
Fragments arranged by size:
1041 599 502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 2142
Size: 878 1264
MscI TGG'CCA
Cuts at: 0 1184 2026 2142
Size: 1184 842 116
Fragments arranged by size:
1184 842 116
MslI CAynn'nnrTG
Cuts at: 0 97 1774 2142
Size: 97 1677 368
Fragments arranged by size:
1677 368 97
MspA1I CmG'CkG
Cuts at: 0 39 289 544 571 647 700 717 816
Size: 39 250 255 27 76 53 17 99
Cuts at: 816 875 1296 1372 1466 1920 1951 2022 2142
Size: 59 421 76 94 454 31 71 120
Fragments arranged by size:
454 421 255 250 120 99 94 76
76 71 59 53 39 31 27 17
NaeI GCC'GGC
Cuts at: 0 765 1383 1800 2142
Size: 765 618 417 342
Fragments arranged by size:
765 618 417 342
NarI GG'CG_CC
Cuts at: 0 19 323 703 1066 2142
Size: 19 304 380 363 1076
Fragments arranged by size:
1076 380 363 304 19
NgoMIV G'CCGG_C
Cuts at: 0 763 1381 1798 2142
Size: 763 618 417 344
Fragments arranged by size:
763 618 417 344
NotI GC'GGCC_GC
Cuts at: 0 284 712 1946 2142
Size: 284 428 1234 196
Fragments arranged by size:
1234 428 284 196
NspI r_CATG'y
Cuts at: 0 1605 1653 1689 2142
Size: 1605 48 36 453
Fragments arranged by size:
1605 453 48 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 831 2142
Size: 204 627 1311
Fragments arranged by size:
1311 627 204
PmlI CAC'GTG
Cuts at: 0 1308 1320 2142
Size: 1308 12 822
Fragments arranged by size:
1308 822 12
PpuMI rG'GwC_Cy
Cuts at: 0 607 886 1164 1494 2142
Size: 607 279 278 330 648
Fragments arranged by size:
648 607 330 279 278
PspOMI G'GGCC_C
Cuts at: 0 558 1048 1099 1390 2104 2142
Size: 558 490 51 291 714 38
Fragments arranged by size:
714 558 490 291 51 38
PspXI vC'TCGA_Gb
Cuts at: 0 1189 2142
Size: 1189 953
PstI C_TGCA'G
Cuts at: 0 50 353 2142
Size: 50 303 1789
Fragments arranged by size:
1789 303 50
PvuII CAG'CTG
Cuts at: 0 39 1296 1920 2142
Size: 39 1257 624 222
Fragments arranged by size:
1257 624 222 39
SacII CC_GC'GG
Cuts at: 0 290 545 572 701 1952 2142
Size: 290 255 27 129 1251 190
Fragments arranged by size:
1251 290 255 190 129 27
SanDI GG'GwC_CC
Cuts at: 0 607 886 2142
Size: 607 279 1256
Fragments arranged by size:
1256 607 279
SapI GCTCTTCn'nnn_
Cuts at: 0 676 1007 2142
Size: 676 331 1135
Fragments arranged by size:
1135 676 331
ScaI AGT'ACT
Cuts at: 0 406 2142
Size: 406 1736
SfcI C'TryA_G
Cuts at: 0 46 349 2142
Size: 46 303 1793
Fragments arranged by size:
1793 303 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1357 1366 1375 1804 2142
Size: 1357 9 9 429 338
Fragments arranged by size:
1357 429 338 9 9
SfoI GGC'GCC
Cuts at: 0 20 324 704 1067 2142
Size: 20 304 380 363 1075
Fragments arranged by size:
1075 380 363 304 20
SmaI CCC'GGG
Cuts at: 0 302 884 1044 2142
Size: 302 582 160 1098
Fragments arranged by size:
1098 582 302 160
SmlI C'TyrA_G
Cuts at: 0 157 388 1189 1621 2142
Size: 157 231 801 432 521
Fragments arranged by size:
801 521 432 231 157
SphI G_CATG'C
Cuts at: 0 1653 2142
Size: 1653 489
StuI AGG'CCT
Cuts at: 0 502 2142
Size: 502 1640
StyI C'CwwG_G
Cuts at: 0 68 1386 1994 2142
Size: 68 1318 608 148
Fragments arranged by size:
1318 608 148 68
TatI w'GTAC_w
Cuts at: 0 404 2142
Size: 404 1738
Tth111I GACn'n_nGTC
Cuts at: 0 1417 2142
Size: 1417 725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2001 2142
Size: 2001 141
XhoI C'TCGA_G
Cuts at: 0 1189 2142
Size: 1189 953
XmaI C'CCGG_G
Cuts at: 0 300 882 1042 2142
Size: 300 582 160 1100
Fragments arranged by size:
1100 582 300 160
XmnI GAAnn'nnTTC
Cuts at: 0 1570 2142
Size: 1570 572
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AlwNI ApaI AvaI
AvrII BanI BanII BbeI BbsI BbvCI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstYI BtgI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI
PmlI PpuMI PspOMI PspXI PstI PvuII SacII SanDI
SapI ScaI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TatI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI AlfI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BcgI BciVI BfrBI BglII BmtI BsaBI BsaXI BsiWI
BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV
FseI HindIII HpaI KpnI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SalI SbfI
SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII TstI XbaI ZraI