Restriction map for
pFN21AB9752
(Linear) (Six-base) MAPSORT of: KIBB9752.seq Check: 4088 from: 1 to: 1821
KIBB9752.orf
With 182 enzymes: SgfI *
July 3, 2012 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1736 1821
Size: 1736 85
AatII G_ACGT'C
Cuts at: 0 247 1821
Size: 247 1574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 754 970 1416 1821
Size: 85 669 216 446 405
Fragments arranged by size:
669 446 405 216 85
AflIII A'CryG_T
Cuts at: 0 1400 1821
Size: 1400 421
AleI CACnn'nnGTG
Cuts at: 0 1393 1821
Size: 1393 428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1118 1821
Size: 1118 703
AlwNI CAG_nnn'CTG
Cuts at: 0 901 1577 1821
Size: 901 676 244
Fragments arranged by size:
901 676 244
ApaI G_GGCC'C
Cuts at: 0 1211 1821
Size: 1211 610
ApaLI G'TGCA_C
Cuts at: 0 808 1580 1723 1821
Size: 808 772 143 98
Fragments arranged by size:
808 772 143 98
ApoI r'AATT_y
Cuts at: 0 1085 1821
Size: 1085 736
AvaI C'yCGr_G
Cuts at: 0 385 690 1821
Size: 385 305 1131
Fragments arranged by size:
1131 385 305
AvrII C'CTAG_G
Cuts at: 0 1773 1821
Size: 1773 48
BanI G'GyrC_C
Cuts at: 0 79 124 1821
Size: 79 45 1697
Fragments arranged by size:
1697 79 45
BanII G_rGCy'C
Cuts at: 0 22 443 1211 1821
Size: 22 421 768 610
Fragments arranged by size:
768 610 421 22
BbeI G_GCGC'C
Cuts at: 0 83 128 1821
Size: 83 45 1693
Fragments arranged by size:
1693 83 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 897 1821
Size: 897 924
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 233 925 959 1503 1537 1821
Size: 199 34 692 34 544 34 284
Fragments arranged by size:
692 544 284 199 34 34 34
BclI T'GATC_A
Cuts at: 0 476 1821
Size: 476 1345
BfrBI ATG'CAT
Cuts at: 0 1036 1821
Size: 1036 785
BglI GCCn_nnn'nGGC
Cuts at: 0 133 173 1821
Size: 133 40 1648
Fragments arranged by size:
1648 133 40
BglII A'GATC_T
Cuts at: 0 524 1821
Size: 524 1297
BlpI GC'TnA_GC
Cuts at: 0 37 1732 1821
Size: 37 1695 89
Fragments arranged by size:
1695 89 37
Bme1580I G_kGCm'C
Cuts at: 0 812 1211 1263 1584 1727 1821
Size: 812 399 52 321 143 94
Fragments arranged by size:
812 399 321 143 94 52
BmgBI CAC'GTC
Cuts at: 0 813 1821
Size: 813 1008
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 1142 1821
Size: 130 1012 679
Fragments arranged by size:
1012 679 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 457 1821
Size: 457 1364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1471 1494 1821
Size: 295 1176 23 327
Fragments arranged by size:
1176 327 295 23
Bpu10I CC'TnA_GC
Cuts at: 0 1266 1821
Size: 1266 555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1784 1821
Size: 28 1756 37
Fragments arranged by size:
1756 37 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 365 1300 1333 1821
Size: 365 935 33 488
Fragments arranged by size:
935 488 365 33
BsaBI GATnn'nnATC
Cuts at: 0 478 962 1821
Size: 478 484 859
Fragments arranged by size:
859 484 478
BsaHI Gr'CG_yC
Cuts at: 0 80 125 244 334 1821
Size: 80 45 119 90 1487
Fragments arranged by size:
1487 119 90 80 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1167 1176 1821
Size: 1167 9 645
Fragments arranged by size:
1167 645 9
BseYI C'CCAG_C
Cuts at: 0 235 1481 1821
Size: 235 1246 340
Fragments arranged by size:
1246 340 235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1793 1821
Size: 48 1745 28
Fragments arranged by size:
1745 48 28
BsiHKAI G_wGCw'C
Cuts at: 0 443 812 1584 1727 1821
Size: 443 369 772 143 94
Fragments arranged by size:
772 443 369 143 94
BsmI GAATG_Cn'
Cuts at: 0 883 1036 1821
Size: 883 153 785
Fragments arranged by size:
883 785 153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 200 827 1821
Size: 200 627 994
Fragments arranged by size:
994 627 200
Bsp1286I G_dGCh'C
Cuts at: 0 22 443 812 1211 1263 1584 1727 1821
Size: 22 421 369 399 52 321 143 94
Fragments arranged by size:
421 399 369 321 143 94 52 22
BspHI T'CATG_A
Cuts at: 0 456 1821
Size: 456 1365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 622 1736 1821
Size: 622 1114 85
Fragments arranged by size:
1114 622 85
BsrBI CCG'CTC
Cuts at: 0 1265 1821
Size: 1265 556
BsrDI GCAATG_nn'
Cuts at: 0 518 557 1107 1521 1821
Size: 518 39 550 414 300
Fragments arranged by size:
550 518 414 300 39
BsrFI r'CCGG_y
Cuts at: 0 231 252 490 1821
Size: 231 21 238 1331
Fragments arranged by size:
1331 238 231 21
BsrGI T'GTAC_A
Cuts at: 0 665 1821
Size: 665 1156
BssHII G'CGCG_C
Cuts at: 0 367 1821
Size: 367 1454
BstAPI GCAn_nnn'nTGC
Cuts at: 0 637 1704 1821
Size: 637 1067 117
Fragments arranged by size:
1067 637 117
BstBI TT'CG_AA
Cuts at: 0 616 1821
Size: 616 1205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1132 1821
Size: 1132 689
BstYI r'GATC_y
Cuts at: 0 423 524 537 1821
Size: 423 101 13 1284
Fragments arranged by size:
1284 423 101 13
BtgI C'CryG_G
Cuts at: 0 146 168 287 415 563 1406 1821
Size: 146 22 119 128 148 843 415
Fragments arranged by size:
843 415 148 146 128 119 22
BtsI GCAGTG_nn'
Cuts at: 0 917 1743 1821
Size: 917 826 78
Fragments arranged by size:
917 826 78
DraIII CAC_nnn'GTG
Cuts at: 0 661 1678 1821
Size: 661 1017 143
Fragments arranged by size:
1017 661 143
EaeI y'GGCC_r
Cuts at: 0 2 1821
Size: 2 1819
EarI CTCTTCn'nnn_
Cuts at: 0 125 1821
Size: 125 1696
EcoICRI GAG'CTC
Cuts at: 0 441 1821
Size: 441 1380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 295 754 970 1416 1471 1494 1821
Size: 85 210 459 216 446 55 23 327
Fragments arranged by size:
459 446 327 216 210 85 55 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1314 1715 1821
Size: 1314 401 106
Fragments arranged by size:
1314 401 106
EcoO109I rG'GnC_Cy
Cuts at: 0 389 411 1207 1667 1769 1821
Size: 389 22 796 460 102 52
Fragments arranged by size:
796 460 389 102 52 22
EcoRI G'AATT_C
Cuts at: 0 1085 1821
Size: 1085 736
EcoRV GAT'ATC
Cuts at: 0 1077 1821
Size: 1077 744
HaeII r_GCGC'y
Cuts at: 0 73 83 128 218 1821
Size: 73 10 45 90 1603
Fragments arranged by size:
1603 90 73 45 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 509 541 701 733 797 829 1821
Size: 509 32 160 32 64 32 992
Fragments arranged by size:
992 509 160 64 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1779 1821
Size: 1779 42
KasI G'GCGC_C
Cuts at: 0 79 124 1821
Size: 79 45 1697
Fragments arranged by size:
1697 79 45
MslI CAynn'nnrTG
Cuts at: 0 472 562 1393 1405 1821
Size: 472 90 831 12 416
Fragments arranged by size:
831 472 416 90 12
MspA1I CmG'CkG
Cuts at: 0 170 289 417 1224 1436 1821
Size: 170 119 128 807 212 385
Fragments arranged by size:
807 385 212 170 128 119
NaeI GCC'GGC
Cuts at: 0 233 254 492 1821
Size: 233 21 238 1329
Fragments arranged by size:
1329 238 233 21
NarI GG'CG_CC
Cuts at: 0 80 125 1821
Size: 80 45 1696
Fragments arranged by size:
1696 80 45
NcoI C'CATG_G
Cuts at: 0 563 1406 1821
Size: 563 843 415
Fragments arranged by size:
843 563 415
NdeI CA'TA_TG
Cuts at: 0 1032 1814 1821
Size: 1032 782 7
Fragments arranged by size:
1032 782 7
NgoMIV G'CCGG_C
Cuts at: 0 231 252 490 1821
Size: 231 21 238 1331
Fragments arranged by size:
1331 238 231 21
NsiI A_TGCA'T
Cuts at: 0 1038 1821
Size: 1038 783
NspI r_CATG'y
Cuts at: 0 36 504 1404 1821
Size: 36 468 900 417
Fragments arranged by size:
900 468 417 36
PciI A'CATG_T
Cuts at: 0 1400 1821
Size: 1400 421
PpuMI rG'GwC_Cy
Cuts at: 0 389 1667 1769 1821
Size: 389 1278 102 52
Fragments arranged by size:
1278 389 102 52
PspOMI G'GGCC_C
Cuts at: 0 1207 1821
Size: 1207 614
PstI C_TGCA'G
Cuts at: 0 166 431 1821
Size: 166 265 1390
Fragments arranged by size:
1390 265 166
PvuII CAG'CTG
Cuts at: 0 1224 1436 1821
Size: 1224 212 385
Fragments arranged by size:
1224 385 212
SacI G_AGCT'C
Cuts at: 0 443 1821
Size: 443 1378
SacII CC_GC'GG
Cuts at: 0 171 290 418 1821
Size: 171 119 128 1403
Fragments arranged by size:
1403 171 128 119
SanDI GG'GwC_CC
Cuts at: 0 389 1821
Size: 389 1432
SfcI C'TryA_G
Cuts at: 0 162 427 1821
Size: 162 265 1394
Fragments arranged by size:
1394 265 162
SfoI GGC'GCC
Cuts at: 0 81 126 1821
Size: 81 45 1695
Fragments arranged by size:
1695 81 45
SmaI CCC'GGG
Cuts at: 0 387 1821
Size: 387 1434
SmlI C'TyrA_G
Cuts at: 0 43 1799 1821
Size: 43 1756 22
Fragments arranged by size:
1756 43 22
SphI G_CATG'C
Cuts at: 0 36 1821
Size: 36 1785
SspI AAT'ATT
Cuts at: 0 553 1821
Size: 553 1268
StuI AGG'CCT
Cuts at: 0 1271 1821
Size: 1271 550
StyI C'CwwG_G
Cuts at: 0 353 485 563 762 1019 1125 1406 1650
Size: 353 132 78 199 257 106 281 244
Cuts at: 1650 1773 1821
Size: 123 48
Fragments arranged by size:
353 281 257 244 199 132 123 106
78 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1399 1821
Size: 1399 422
TatI w'GTAC_w
Cuts at: 0 665 1821
Size: 665 1156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 1821
Size: 750 1071
XmaI C'CCGG_G
Cuts at: 0 385 1821
Size: 385 1436
ZraI GAC'GTC
Cuts at: 0 245 1821
Size: 245 1576
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HindIII
KasI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SfcI SfoI SmaI SmlI SphI SspI
StuI StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI BaeI BamHI BbvCI BciVI BmtI
BsaAI BsaWI BsaXI BsiEI BsiWI BspEI BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI MscI NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI
TstI Tth111I XbaI XhoI XmnI