Restriction map for
pFN21AB9724
(Linear) (Six-base) MAPSORT of: KIBB9724.seq Check: 8166 from: 1 to: 1254
KIBB9724.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 702 1254
Size: 702 552
AfeI AGC'GCT
Cuts at: 0 817 1099 1254
Size: 817 282 155
Fragments arranged by size:
817 282 155
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 645 1254
Size: 613 32 609
Fragments arranged by size:
613 609 32
ApaLI G'TGCA_C
Cuts at: 0 267 1254
Size: 267 987
ApoI r'AATT_y
Cuts at: 0 1237 1254
Size: 1237 17
AvaI C'yCGr_G
Cuts at: 0 435 453 471 484 507 528 646 799
Size: 435 18 18 13 23 21 118 153
Cuts at: 799 891 994 1090 1105 1254
Size: 92 103 96 15 149
Fragments arranged by size:
435 153 149 118 103 96 92 23
21 18 18 15 13
BanI G'GyrC_C
Cuts at: 0 285 421 1009 1254
Size: 285 136 588 245
Fragments arranged by size:
588 285 245 136
BanII G_rGCy'C
Cuts at: 0 542 800 868 904 1043 1254
Size: 542 258 68 36 139 211
Fragments arranged by size:
542 258 211 139 68 36
BbeI G_GCGC'C
Cuts at: 0 425 1013 1254
Size: 425 588 241
Fragments arranged by size:
588 425 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 154 707 741 1254
Size: 120 34 553 34 513
Fragments arranged by size:
553 513 120 34 34
BfrBI ATG'CAT
Cuts at: 0 39 1254
Size: 39 1215
BglI GCCn_nnn'nGGC
Cuts at: 0 440 458 476 512 533 633 1254
Size: 440 18 18 36 21 100 621
Fragments arranged by size:
621 440 100 36 21 18 18
Bme1580I G_kGCm'C
Cuts at: 0 271 1254
Size: 271 983
BmgBI CAC'GTC
Cuts at: 0 272 861 1254
Size: 272 589 393
Fragments arranged by size:
589 393 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1116 1254
Size: 1116 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1254
Size: 63 1191
Bpu10I CC'TnA_GC
Cuts at: 0 53 1037 1254
Size: 53 984 217
Fragments arranged by size:
984 217 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 955 1254
Size: 868 87 299
Fragments arranged by size:
868 299 87
BsaAI yAC'GTr
Cuts at: 0 242 1254
Size: 242 1012
BsaHI Gr'CG_yC
Cuts at: 0 236 422 763 1010 1254
Size: 236 186 341 247 244
Fragments arranged by size:
341 247 244 236 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 340 370 1254
Size: 67 30 243 30 884
Fragments arranged by size:
884 243 67 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 348 1012 1254
Size: 348 664 242
Fragments arranged by size:
664 348 242
BseYI C'CCAG_C
Cuts at: 0 840 1013 1254
Size: 840 173 241
Fragments arranged by size:
840 241 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1144 1254
Size: 58 1086 110
Fragments arranged by size:
1086 110 58
BsiEI CG_ry'CG
Cuts at: 0 188 410 614 707 740 1254
Size: 188 222 204 93 33 514
Fragments arranged by size:
514 222 204 188 93 33
BsiHKAI G_wGCw'C
Cuts at: 0 59 271 800 919 1043 1254
Size: 59 212 529 119 124 211
Fragments arranged by size:
529 212 211 124 119 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 148 1254
Size: 148 1106
Bsp1286I G_dGCh'C
Cuts at: 0 59 271 542 800 868 904 919 1043
Size: 59 212 271 258 68 36 15 124
Cuts at: 1043 1254
Size: 211
Fragments arranged by size:
271 258 212 211 124 68 59 36
15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 702 917 1254
Size: 702 215 337
Fragments arranged by size:
702 337 215
BsrFI r'CCGG_y
Cuts at: 0 382 409 1254
Size: 382 27 845
Fragments arranged by size:
845 382 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 923 1254
Size: 923 331
BstYI r'GATC_y
Cuts at: 0 887 1254
Size: 887 367
BtgI C'CryG_G
Cuts at: 0 188 281 602 672 740 1254
Size: 188 93 321 70 68 514
Fragments arranged by size:
514 321 188 93 70 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 180 1065 1143 1254
Size: 180 885 78 111
Fragments arranged by size:
885 180 111 78
EaeI y'GGCC_r
Cuts at: 0 185 380 407 611 704 737 875 1254
Size: 185 195 27 204 93 33 138 379
Fragments arranged by size:
379 204 195 185 138 93 33 27
EagI C'GGCC_G
Cuts at: 0 185 407 611 704 737 1254
Size: 185 222 204 93 33 517
Fragments arranged by size:
517 222 204 185 93 33
EarI CTCTTCn'nnn_
Cuts at: 0 142 1048 1254
Size: 142 906 206
Fragments arranged by size:
906 206 142
EcoICRI GAG'CTC
Cuts at: 0 798 1041 1254
Size: 798 243 213
Fragments arranged by size:
798 243 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1254
Size: 63 1191
HaeII r_GCGC'y
Cuts at: 0 181 328 425 819 874 1013 1101 1254
Size: 181 147 97 394 55 139 88 153
Fragments arranged by size:
394 181 153 147 139 97 88 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 850 882 1254
Size: 340 32 478 32 372
Fragments arranged by size:
478 372 340 32 32
KasI G'GCGC_C
Cuts at: 0 421 1009 1254
Size: 421 588 245
Fragments arranged by size:
588 421 245
MscI TGG'CCA
Cuts at: 0 877 1254
Size: 877 377
MslI CAynn'nnrTG
Cuts at: 0 199 622 1254
Size: 199 423 632
Fragments arranged by size:
632 423 199
MspA1I CmG'CkG
Cuts at: 0 123 604 685 691 721 754 828 1254
Size: 123 481 81 6 30 33 74 426
Fragments arranged by size:
481 426 123 81 74 33 30 6
NaeI GCC'GGC
Cuts at: 0 384 411 1254
Size: 384 27 843
Fragments arranged by size:
843 384 27
NarI GG'CG_CC
Cuts at: 0 422 1010 1254
Size: 422 588 244
Fragments arranged by size:
588 422 244
NdeI CA'TA_TG
Cuts at: 0 572 1254
Size: 572 682
NgoMIV G'CCGG_C
Cuts at: 0 382 409 1254
Size: 382 27 845
Fragments arranged by size:
845 382 27
NotI GC'GGCC_GC
Cuts at: 0 611 1254
Size: 611 643
NsiI A_TGCA'T
Cuts at: 0 41 1254
Size: 41 1213
NspI r_CATG'y
Cuts at: 0 627 1254
Size: 627 627
PasI CC'CwG_GG
Cuts at: 0 207 1254
Size: 207 1047
PspXI vC'TCGA_Gb
Cuts at: 0 799 994 1105 1254
Size: 799 195 111 149
Fragments arranged by size:
799 195 149 111
PstI C_TGCA'G
Cuts at: 0 128 928 986 1254
Size: 128 800 58 268
Fragments arranged by size:
800 268 128 58
SacI G_AGCT'C
Cuts at: 0 800 1043 1254
Size: 800 243 211
Fragments arranged by size:
800 243 211
SacII CC_GC'GG
Cuts at: 0 605 1254
Size: 605 649
SapI GCTCTTCn'nnn_
Cuts at: 0 1048 1254
Size: 1048 206
SbfI CC_TGCA'GG
Cuts at: 0 986 1254
Size: 986 268
SfcI C'TryA_G
Cuts at: 0 124 924 982 1254
Size: 124 800 58 272
Fragments arranged by size:
800 272 124 58
SfoI GGC'GCC
Cuts at: 0 423 1011 1254
Size: 423 588 243
Fragments arranged by size:
588 423 243
SmaI CCC'GGG
Cuts at: 0 437 455 473 486 509 530 648 893
Size: 437 18 18 13 23 21 118 245
Cuts at: 893 1254
Size: 361
Fragments arranged by size:
437 361 245 118 23 21 18 18
13
SmlI C'TyrA_G
Cuts at: 0 799 883 970 994 1105 1254
Size: 799 84 87 24 111 149
Fragments arranged by size:
799 149 111 87 84 24
SrfI GCCC'GGGC
Cuts at: 0 455 473 530 1254
Size: 455 18 57 724
Fragments arranged by size:
724 455 57 18
StuI AGG'CCT
Cuts at: 0 1111 1254
Size: 1111 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 1254
Size: 67 32 1155
Fragments arranged by size:
1155 67 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 1254
Size: 288 966
XhoI C'TCGA_G
Cuts at: 0 799 994 1105 1254
Size: 799 195 111 149
Fragments arranged by size:
799 195 149 111
XmaI C'CCGG_G
Cuts at: 0 435 453 471 484 507 528 646 891
Size: 435 18 18 13 23 21 118 245
Cuts at: 891 1254
Size: 363
Fragments arranged by size:
435 363 245 118 23 21 18 18
13
Enzymes that do cut:
AarI AfeI AloI ApaLI ApoI AvaI BanI BanII
BbeI BcgI BfrBI BglI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstYI
BtgI BtgZI EaeI EagI EarI EcoICRI Eco57MI HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NotI NsiI NspI PasI PspXI PstI SacI
SacII SapI SbfI SfcI SfoI SmaI SmlI SrfI
StuI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglII
BlpI BmrI BmtI BsaI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NheI NruI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI StyI
SwaI TaqII TatI Tth111I XbaI XmnI ZraI