Restriction map for
pFN21AB9719
(Linear) (Six-base) MAPSORT of: KIBB9719.seq Check: 3632 from: 1 to: 1206
KIBB9719.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 359 1206
Size: 306 53 847
Fragments arranged by size:
847 306 53
AccI GT'mk_AC
Cuts at: 0 1190 1206
Size: 1190 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 550 679 1206
Size: 207 343 129 527
Fragments arranged by size:
527 343 207 129
AfeI AGC'GCT
Cuts at: 0 217 268 537 682 712 1206
Size: 217 51 269 145 30 494
Fragments arranged by size:
494 269 217 145 51 30
AleI CACnn'nnGTG
Cuts at: 0 316 1206
Size: 316 890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 377 1206
Size: 377 829
AlwNI CAG_nnn'CTG
Cuts at: 0 194 1206
Size: 194 1012
ApaI G_GGCC'C
Cuts at: 0 25 817 1079 1206
Size: 25 792 262 127
Fragments arranged by size:
792 262 127 25
AscI GG'CGCG_CC
Cuts at: 0 562 1206
Size: 562 644
AvaI C'yCGr_G
Cuts at: 0 29 873 1059 1206
Size: 29 844 186 147
Fragments arranged by size:
844 186 147 29
BanII G_rGCy'C
Cuts at: 0 25 817 882 1068 1079 1206
Size: 25 792 65 186 11 127
Fragments arranged by size:
792 186 127 65 25 11
BbvCI CC'TCA_GC
Cuts at: 0 773 1206
Size: 773 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1141 1175 1206
Size: 1141 34 31
Fragments arranged by size:
1141 34 31
BglI GCCn_nnn'nGGC
Cuts at: 0 299 917 998 1206
Size: 299 618 81 208
Fragments arranged by size:
618 299 208 81
Bme1580I G_kGCm'C
Cuts at: 0 25 817 1058 1079 1206
Size: 25 792 241 21 127
Fragments arranged by size:
792 241 127 25 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1106 1206
Size: 1106 100
BmtI G_CTAG'C
Cuts at: 0 61 1206
Size: 61 1145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 600 1206
Size: 600 606
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 919 1002 1206
Size: 541 378 83 204
Fragments arranged by size:
541 378 204 83
Bpu10I CC'TnA_GC
Cuts at: 0 773 1206
Size: 773 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 166 1206
Size: 130 36 1040
Fragments arranged by size:
1040 130 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 783 1206
Size: 783 423
BsaAI yAC'GTr
Cuts at: 0 570 1206
Size: 570 636
BsaHI Gr'CG_yC
Cuts at: 0 668 1206
Size: 668 538
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 1206
Size: 1003 203
BseYI C'CCAG_C
Cuts at: 0 18 297 683 1004 1206
Size: 18 279 386 321 202
Fragments arranged by size:
386 321 279 202 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 337 637 1206
Size: 207 130 300 569
Fragments arranged by size:
569 300 207 130
BsiEI CG_ry'CG
Cuts at: 0 493 675 1206
Size: 493 182 531
Fragments arranged by size:
531 493 182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 517 586 1206
Size: 517 69 620
Fragments arranged by size:
620 517 69
Bsp1286I G_dGCh'C
Cuts at: 0 25 817 882 1058 1068 1079 1206
Size: 25 792 65 176 10 11 127
Fragments arranged by size:
792 176 127 65 25 11 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 92 306 359 609 1206
Size: 59 33 214 53 250 597
Fragments arranged by size:
597 250 214 59 53 33
BsrFI r'CCGG_y
Cuts at: 0 425 558 670 674 866 1206
Size: 425 133 112 4 192 340
Fragments arranged by size:
425 340 192 133 112 4
BssHII G'CGCG_C
Cuts at: 0 7 562 1206
Size: 7 555 644
Fragments arranged by size:
644 555 7
BssSI C'ACGA_G
Cuts at: 0 430 1206
Size: 430 776
BstAPI GCAn_nnn'nTGC
Cuts at: 0 194 321 1206
Size: 194 127 885
Fragments arranged by size:
885 194 127
BstEII G'GTnAC_C
Cuts at: 0 1144 1206
Size: 1144 62
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 519 1206
Size: 318 201 687
Fragments arranged by size:
687 318 201
BstYI r'GATC_y
Cuts at: 0 95 1206
Size: 95 1111
BtgI C'CryG_G
Cuts at: 0 392 479 717 1127 1206
Size: 392 87 238 410 79
Fragments arranged by size:
410 392 238 87 79
BtsI GCAGTG_nn'
Cuts at: 0 61 354 1206
Size: 61 293 852
Fragments arranged by size:
852 293 61
DrdI GACnn_nn'nnGTC
Cuts at: 0 775 1206
Size: 775 431
EaeI y'GGCC_r
Cuts at: 0 209 389 482 490 672 818 1206
Size: 209 180 93 8 182 146 388
Fragments arranged by size:
388 209 182 180 146 93 8
EagI C'GGCC_G
Cuts at: 0 490 672 1206
Size: 490 182 534
Fragments arranged by size:
534 490 182
EarI CTCTTCn'nnn_
Cuts at: 0 526 646 1206
Size: 526 120 560
Fragments arranged by size:
560 526 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 457 820 1206
Size: 94 363 363 386
Fragments arranged by size:
386 363 363 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 541 550 679 919 1002 1206
Size: 207 334 9 129 240 83 204
Fragments arranged by size:
334 240 207 204 129 83 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 1206
Size: 415 791
EcoO109I rG'GnC_Cy
Cuts at: 0 1076 1206
Size: 1076 130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 273 1206
Size: 273 933
FseI GG_CCGG'CC
Cuts at: 0 870 1206
Size: 870 336
HaeII r_GCGC'y
Cuts at: 0 219 270 539 684 714 1206
Size: 219 51 269 145 30 492
Fragments arranged by size:
492 269 219 145 51 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1206
Size: 713 493
MscI TGG'CCA
Cuts at: 0 391 1206
Size: 391 815
MslI CAynn'nnrTG
Cuts at: 0 316 610 1090 1206
Size: 316 294 480 116
Fragments arranged by size:
480 316 294 116
MspA1I CmG'CkG
Cuts at: 0 102 297 387 519 719 1139 1206
Size: 102 195 90 132 200 420 67
Fragments arranged by size:
420 200 195 132 102 90 67
NaeI GCC'GGC
Cuts at: 0 427 560 672 676 868 1206
Size: 427 133 112 4 192 338
Fragments arranged by size:
427 338 192 133 112 4
NgoMIV G'CCGG_C
Cuts at: 0 425 558 670 674 866 1206
Size: 425 133 112 4 192 340
Fragments arranged by size:
425 340 192 133 112 4
NheI G'CTAG_C
Cuts at: 0 57 1206
Size: 57 1149
NotI GC'GGCC_GC
Cuts at: 0 490 1206
Size: 490 716
NspI r_CATG'y
Cuts at: 0 609 1206
Size: 609 597
PasI CC'CwG_GG
Cuts at: 0 882 1206
Size: 882 324
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1206
Size: 861 345
PfoI T'CCnGG_A
Cuts at: 0 128 1206
Size: 128 1078
PmlI CAC'GTG
Cuts at: 0 570 1206
Size: 570 636
PspOMI G'GGCC_C
Cuts at: 0 21 813 1075 1206
Size: 21 792 262 131
Fragments arranged by size:
792 262 131 21
PvuII CAG'CTG
Cuts at: 0 297 387 519 1206
Size: 297 90 132 687
Fragments arranged by size:
687 297 132 90
SacII CC_GC'GG
Cuts at: 0 720 1206
Size: 720 486
SapI GCTCTTCn'nnn_
Cuts at: 0 526 1206
Size: 526 680
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 917 1206
Size: 917 289
SmaI CCC'GGG
Cuts at: 0 31 1206
Size: 31 1175
SmlI C'TyrA_G
Cuts at: 0 145 181 1206
Size: 145 36 1025
Fragments arranged by size:
1025 145 36
StuI AGG'CCT
Cuts at: 0 859 911 1206
Size: 859 52 295
Fragments arranged by size:
859 295 52
StyI C'CwwG_G
Cuts at: 0 644 800 860 1206
Size: 644 156 60 346
Fragments arranged by size:
644 346 156 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1098 1206
Size: 868 230 108
Fragments arranged by size:
868 230 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 1092 1206
Size: 858 234 114
Fragments arranged by size:
858 234 114
XmaI C'CCGG_G
Cuts at: 0 29 1206
Size: 29 1177
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AlwNI ApaI
AscI AvaI BanII BbvCI BcgI BglI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI FseI HaeII MmeI MscI MslI MspA1I NaeI
NgoMIV NheI NotI NspI PasI PflMI PfoI PmlI
PspOMI PvuII SacII SapI SfiI SmaI SmlI StuI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI
BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EcoICRI EcoRI EcoRV FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NruI NsiI PacI PciI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI