Restriction map for
pFN21AB9715
(Linear) (Six-base) MAPSORT of: KIBB9715.seq Check: 1454 from: 1 to: 1176
KIBB9715.orf
With 182 enzymes: SgfI *
March 22, 2012 15:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1176
Size: 901 275
AflIII A'CryG_T
Cuts at: 0 835 1176
Size: 835 341
AgeI A'CCGG_T
Cuts at: 0 667 1176
Size: 667 509
AlwNI CAG_nnn'CTG
Cuts at: 0 378 496 1005 1128 1176
Size: 378 118 509 123 48
Fragments arranged by size:
509 378 123 118 48
ApaI G_GGCC'C
Cuts at: 0 779 1176
Size: 779 397
AvaI C'yCGr_G
Cuts at: 0 482 1176
Size: 482 694
BanI G'GyrC_C
Cuts at: 0 100 183 261 478 486 715 1176
Size: 100 83 78 217 8 229 461
Fragments arranged by size:
461 229 217 100 83 78 8
BanII G_rGCy'C
Cuts at: 0 703 763 779 1176
Size: 703 60 16 397
Fragments arranged by size:
703 397 60 16
BbeI G_GCGC'C
Cuts at: 0 104 187 265 490 1176
Size: 104 83 78 225 686
Fragments arranged by size:
686 225 104 83 78
BbvCI CC'TCA_GC
Cuts at: 0 79 248 1176
Size: 79 169 928
Fragments arranged by size:
928 169 79
BglI GCCn_nnn'nGGC
Cuts at: 0 619 1176
Size: 619 557
BglII A'GATC_T
Cuts at: 0 1043 1176
Size: 1043 133
BlpI GC'TnA_GC
Cuts at: 0 762 1176
Size: 762 414
Bme1580I G_kGCm'C
Cuts at: 0 481 718 779 1176
Size: 481 237 61 397
Fragments arranged by size:
481 397 237 61
Bpu10I CC'TnA_GC
Cuts at: 0 79 85 248 1176
Size: 79 6 163 928
Fragments arranged by size:
928 163 79 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1176
Size: 937 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 1176
Size: 452 724
BsaBI GATnn'nnATC
Cuts at: 0 1042 1176
Size: 1042 134
BsaHI Gr'CG_yC
Cuts at: 0 101 184 262 487 1176
Size: 101 83 78 225 689
Fragments arranged by size:
689 225 101 83 78
BsaWI w'CCGG_w
Cuts at: 0 667 859 1176
Size: 667 192 317
Fragments arranged by size:
667 317 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 125 155 407 437 1123 1153 1176
Size: 125 30 252 30 686 30 23
Fragments arranged by size:
686 252 125 30 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 413 425 428 1176
Size: 413 12 3 748
Fragments arranged by size:
748 413 12 3
BseYI C'CCAG_C
Cuts at: 0 290 548 583 1176
Size: 290 258 35 593
Fragments arranged by size:
593 290 258 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 816 1093 1176
Size: 280 536 277 83
Fragments arranged by size:
536 280 277 83
BsiEI CG_ry'CG
Cuts at: 0 614 644 916 935 1176
Size: 614 30 272 19 241
Fragments arranged by size:
614 272 241 30 19
BsiHKAI G_wGCw'C
Cuts at: 0 734 1176
Size: 734 442
Bsp1286I G_dGCh'C
Cuts at: 0 481 703 718 734 763 779 1176
Size: 481 222 15 16 29 16 397
Fragments arranged by size:
481 397 222 29 16 16 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 442 1176
Size: 442 734
BsrFI r'CCGG_y
Cuts at: 0 144 562 667 1176
Size: 144 418 105 509
Fragments arranged by size:
509 418 144 105
BsrGI T'GTAC_A
Cuts at: 0 1130 1176
Size: 1130 46
BssHII G'CGCG_C
Cuts at: 0 1062 1176
Size: 1062 114
BstYI r'GATC_y
Cuts at: 0 1043 1176
Size: 1043 133
Bsu36I CC'TnA_GG
Cuts at: 0 331 1176
Size: 331 845
BtgI C'CryG_G
Cuts at: 0 179 614 779 916 1148 1176
Size: 179 435 165 137 232 28
Fragments arranged by size:
435 232 179 165 137 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 143 1176
Size: 143 1033
BtsI GCAGTG_nn'
Cuts at: 0 629 687 1176
Size: 629 58 489
Fragments arranged by size:
629 489 58
EaeI y'GGCC_r
Cuts at: 0 392 611 641 913 932 1176
Size: 392 219 30 272 19 244
Fragments arranged by size:
392 272 244 219 30 19
EagI C'GGCC_G
Cuts at: 0 611 641 913 932 1176
Size: 611 30 272 19 244
Fragments arranged by size:
611 272 244 30 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 1176
Size: 973 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1176
Size: 901 275
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1009 1176
Size: 1009 167
HaeII r_GCGC'y
Cuts at: 0 104 187 265 490 497 1108 1176
Size: 104 83 78 225 7 611 68
Fragments arranged by size:
611 225 104 83 78 68 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 407 439 449 481 838 870
Size: 78 32 297 32 10 32 357 32
Cuts at: 870 1176
Size: 306
Fragments arranged by size:
357 306 297 78 32 32 32 32
10
KasI G'GCGC_C
Cuts at: 0 100 183 261 486 1176
Size: 100 83 78 225 690
Fragments arranged by size:
690 225 100 83 78
MspA1I CmG'CkG
Cuts at: 0 107 116 122 181 286 294 616 918
Size: 107 9 6 59 105 8 322 302
Cuts at: 918 942 993 1176
Size: 24 51 183
Fragments arranged by size:
322 302 183 107 105 59 51 24
9 8 6
NaeI GCC'GGC
Cuts at: 0 564 1176
Size: 564 612
NarI GG'CG_CC
Cuts at: 0 101 184 262 487 1176
Size: 101 83 78 225 689
Fragments arranged by size:
689 225 101 83 78
NgoMIV G'CCGG_C
Cuts at: 0 562 1176
Size: 562 614
NotI GC'GGCC_GC
Cuts at: 0 611 641 913 1176
Size: 611 30 272 263
Fragments arranged by size:
611 272 263 30
PasI CC'CwG_GG
Cuts at: 0 1122 1176
Size: 1122 54
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 1176
Size: 474 702
PfoI T'CCnGG_A
Cuts at: 0 1154 1176
Size: 1154 22
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1007 1039 1176
Size: 1007 32 137
Fragments arranged by size:
1007 137 32
PspOMI G'GGCC_C
Cuts at: 0 775 1176
Size: 775 401
PstI C_TGCA'G
Cuts at: 0 947 1176
Size: 947 229
PvuII CAG'CTG
Cuts at: 0 294 942 1176
Size: 294 648 234
Fragments arranged by size:
648 294 234
RsrII CG'GwC_CG
Cuts at: 0 862 920 1176
Size: 862 58 256
Fragments arranged by size:
862 256 58
SacII CC_GC'GG
Cuts at: 0 182 617 919 1176
Size: 182 435 302 257
Fragments arranged by size:
435 302 257 182
SfcI C'TryA_G
Cuts at: 0 943 1176
Size: 943 233
SfoI GGC'GCC
Cuts at: 0 102 185 263 488 1176
Size: 102 83 78 225 688
Fragments arranged by size:
688 225 102 83 78
SgrAI Cr'CCGG_yG
Cuts at: 0 562 667 1176
Size: 562 105 509
Fragments arranged by size:
562 509 105
SmaI CCC'GGG
Cuts at: 0 484 1176
Size: 484 692
SmlI C'TyrA_G
Cuts at: 0 952 1176
Size: 952 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1176
Size: 813 363
TatI w'GTAC_w
Cuts at: 0 827 1130 1176
Size: 827 303 46
Fragments arranged by size:
827 303 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 1176
Size: 125 32 1019
Fragments arranged by size:
1019 125 32
Tth111I GACn'n_nGTC
Cuts at: 0 463 1176
Size: 463 713
XmaI C'CCGG_G
Cuts at: 0 482 1176
Size: 482 694
Enzymes that do cut:
AcuI AflIII AgeI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BglI BglII BlpI Bme1580I Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EciI Eco57MI
EcoNI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV
NotI PasI PflMI PfoI PpiI PspOMI PstI PvuII
RsrII SacII SfcI SfoI SgrAI SmaI SmlI TaqII
TatI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BplI BpmI BsaAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NruI NsiI NspI
PacI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI XbaI XcmI XhoI XmnI ZraI