Restriction map for
pFN21AB9711
(Linear) (Six-base) MAPSORT of: KIBB9711.seq Check: 7549 from: 1 to: 1101
KIBB9711.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1091 1101
Size: 1091 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 822 1101
Size: 70 752 279
Fragments arranged by size:
752 279 70
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 787 883 1003 1101
Size: 787 96 120 98
Fragments arranged by size:
787 120 98 96
AlwNI CAG_nnn'CTG
Cuts at: 0 234 522 591 1101
Size: 234 288 69 510
Fragments arranged by size:
510 288 234 69
ApaI G_GGCC'C
Cuts at: 0 346 1101
Size: 346 755
ApaLI G'TGCA_C
Cuts at: 0 497 1101
Size: 497 604
AvaI C'yCGr_G
Cuts at: 0 133 646 1101
Size: 133 513 455
Fragments arranged by size:
513 455 133
BamHI G'GATC_C
Cuts at: 0 632 1101
Size: 632 469
BanI G'GyrC_C
Cuts at: 0 54 541 965 1101
Size: 54 487 424 136
Fragments arranged by size:
487 424 136 54
BanII G_rGCy'C
Cuts at: 0 10 280 346 1101
Size: 10 270 66 755
Fragments arranged by size:
755 270 66 10
BbeI G_GCGC'C
Cuts at: 0 58 1101
Size: 58 1043
BbvCI CC'TCA_GC
Cuts at: 0 23 1101
Size: 23 1078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 1101
Size: 114 34 953
Fragments arranged by size:
953 114 34
BclI T'GATC_A
Cuts at: 0 263 1101
Size: 263 838
BglI GCCn_nnn'nGGC
Cuts at: 0 719 749 1101
Size: 719 30 352
Fragments arranged by size:
719 352 30
Bme1580I G_kGCm'C
Cuts at: 0 346 501 647 1101
Size: 346 155 146 454
Fragments arranged by size:
454 346 155 146
BmgBI CAC'GTC
Cuts at: 0 317 1101
Size: 317 784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1101
Size: 544 557
Bpu10I CC'TnA_GC
Cuts at: 0 23 1101
Size: 23 1078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1101
Size: 22 1079
BsaAI yAC'GTr
Cuts at: 0 378 502 867 1101
Size: 378 124 365 234
Fragments arranged by size:
378 365 234 124
BsaHI Gr'CG_yC
Cuts at: 0 55 1101
Size: 55 1046
BsaWI w'CCGG_w
Cuts at: 0 470 1101
Size: 470 631
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 1101
Size: 670 431
BseYI C'CCAG_C
Cuts at: 0 976 1101
Size: 976 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 247 859 1101
Size: 82 165 612 242
Fragments arranged by size:
612 242 165 82
BsiEI CG_ry'CG
Cuts at: 0 117 357 973 1101
Size: 117 240 616 128
Fragments arranged by size:
616 240 128 117
BsiHKAI G_wGCw'C
Cuts at: 0 17 38 134 280 377 501 1101
Size: 17 21 96 146 97 124 600
Fragments arranged by size:
600 146 124 97 96 21 17
Bsp1286I G_dGCh'C
Cuts at: 0 10 17 38 134 280 346 377 501
Size: 10 7 21 96 146 66 31 124
Cuts at: 501 647 1101
Size: 146 454
Fragments arranged by size:
454 146 146 124 96 66 31 21
10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1091 1101
Size: 779 312 10
Fragments arranged by size:
779 312 10
BsrFI r'CCGG_y
Cuts at: 0 352 934 968 1086 1101
Size: 352 582 34 118 15
Fragments arranged by size:
582 352 118 34 15
BssHII G'CGCG_C
Cuts at: 0 942 959 1101
Size: 942 17 142
Fragments arranged by size:
942 142 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 731 1083 1101
Size: 731 352 18
Fragments arranged by size:
731 352 18
BstYI r'GATC_y
Cuts at: 0 632 1101
Size: 632 469
BtgI C'CryG_G
Cuts at: 0 716 747 1091 1101
Size: 716 31 344 10
Fragments arranged by size:
716 344 31 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 252 744 1101
Size: 252 492 357
Fragments arranged by size:
492 357 252
ClaI AT'CG_AT
Cuts at: 0 449 1101
Size: 449 652
EaeI y'GGCC_r
Cuts at: 0 71 114 354 932 970 1088 1101
Size: 71 43 240 578 38 118 13
Fragments arranged by size:
578 240 118 71 43 38 13
EagI C'GGCC_G
Cuts at: 0 114 354 970 1101
Size: 114 240 616 131
Fragments arranged by size:
616 240 131 114
EarI CTCTTCn'nnn_
Cuts at: 0 151 949 1101
Size: 151 798 152
Fragments arranged by size:
798 152 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 712 1101
Size: 712 389
EcoICRI GAG'CTC
Cuts at: 0 278 1101
Size: 278 823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 544 822 1101
Size: 70 474 278 279
Fragments arranged by size:
474 279 278 70
EcoO109I rG'GnC_Cy
Cuts at: 0 27 294 343 1101
Size: 27 267 49 758
Fragments arranged by size:
758 267 49 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 1101
Size: 201 900
FseI GG_CCGG'CC
Cuts at: 0 938 1101
Size: 938 163
FspI TGC'GCA
Cuts at: 0 787 859 1101
Size: 787 72 242
Fragments arranged by size:
787 242 72
FspAI rTGC'GCAy
Cuts at: 0 787 1101
Size: 787 314
HaeII r_GCGC'y
Cuts at: 0 58 70 891 920 1101
Size: 58 12 821 29 181
Fragments arranged by size:
821 181 58 29 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 722 754 1101
Size: 255 32 435 32 347
Fragments arranged by size:
435 347 255 32 32
KasI G'GCGC_C
Cuts at: 0 54 1101
Size: 54 1047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1101
Size: 113 988
MslI CAynn'nnrTG
Cuts at: 0 568 1101
Size: 568 533
MspA1I CmG'CkG
Cuts at: 0 119 718 749 930 1101
Size: 119 599 31 181 171
Fragments arranged by size:
599 181 171 119 31
NaeI GCC'GGC
Cuts at: 0 936 1088 1101
Size: 936 152 13
Fragments arranged by size:
936 152 13
NarI GG'CG_CC
Cuts at: 0 55 1101
Size: 55 1046
NcoI C'CATG_G
Cuts at: 0 1091 1101
Size: 1091 10
NgoMIV G'CCGG_C
Cuts at: 0 934 1086 1101
Size: 934 152 15
Fragments arranged by size:
934 152 15
NspI r_CATG'y
Cuts at: 0 729 786 1101
Size: 729 57 315
Fragments arranged by size:
729 315 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 317 798 1101
Size: 317 481 303
Fragments arranged by size:
481 317 303
PmlI CAC'GTG
Cuts at: 0 378 867 1101
Size: 378 489 234
Fragments arranged by size:
489 378 234
PspOMI G'GGCC_C
Cuts at: 0 342 1101
Size: 342 759
PstI C_TGCA'G
Cuts at: 0 992 1101
Size: 992 109
PvuII CAG'CTG
Cuts at: 0 930 1101
Size: 930 171
SacI G_AGCT'C
Cuts at: 0 280 1101
Size: 280 821
SacII CC_GC'GG
Cuts at: 0 719 750 1101
Size: 719 31 351
Fragments arranged by size:
719 351 31
SapI GCTCTTCn'nnn_
Cuts at: 0 151 949 1101
Size: 151 798 152
Fragments arranged by size:
798 152 151
SfcI C'TryA_G
Cuts at: 0 988 1101
Size: 988 113
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 719 1101
Size: 719 382
SfoI GGC'GCC
Cuts at: 0 56 1101
Size: 56 1045
SmaI CCC'GGG
Cuts at: 0 648 1101
Size: 648 453
SmlI C'TyrA_G
Cuts at: 0 37 1101
Size: 37 1064
SphI G_CATG'C
Cuts at: 0 729 1101
Size: 729 372
SrfI GCCC'GGGC
Cuts at: 0 648 1101
Size: 648 453
StyI C'CwwG_G
Cuts at: 0 1091 1101
Size: 1091 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 624 656 1101
Size: 624 32 445
Fragments arranged by size:
624 445 32
XmaI C'CCGG_G
Cuts at: 0 646 1101
Size: 646 455
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BclI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstAPI BstYI BtgI BtgZI ClaI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FseI FspI FspAI
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PmlI PspOMI PstI PvuII
SacI SacII SapI SfcI SfiI SfoI SmaI SmlI
SphI SrfI StyI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BciVI BfrBI BglII BlpI BmrI BmtI
BplI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI EcoNI
EcoRI EcoRV HincII HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmnI ZraI