Restriction map for
pFN21AB9686
(Linear) (Six-base) MAPSORT of: KIBB9686.seq Check: 1376 from: 1 to: 597
KIBB9686.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AflIII A'CryG_T
Cuts at: 0 95 597
Size: 95 502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 597
Size: 94 32 471
Fragments arranged by size:
471 94 32
ApaI G_GGCC'C
Cuts at: 0 130 157 199 597
Size: 130 27 42 398
Fragments arranged by size:
398 130 42 27
AvaI C'yCGr_G
Cuts at: 0 150 157 597
Size: 150 7 440
Fragments arranged by size:
440 150 7
BanI G'GyrC_C
Cuts at: 0 445 597
Size: 445 152
BanII G_rGCy'C
Cuts at: 0 130 157 199 597
Size: 130 27 42 398
Fragments arranged by size:
398 130 42 27
BbeI G_GCGC'C
Cuts at: 0 449 597
Size: 449 148
BbvCI CC'TCA_GC
Cuts at: 0 505 597
Size: 505 92
BglI GCCn_nnn'nGGC
Cuts at: 0 161 277 597
Size: 161 116 320
Fragments arranged by size:
320 161 116
BlpI GC'TnA_GC
Cuts at: 0 61 597
Size: 61 536
Bme1580I G_kGCm'C
Cuts at: 0 130 157 199 597
Size: 130 27 42 398
Fragments arranged by size:
398 130 42 27
BmgBI CAC'GTC
Cuts at: 0 51 597
Size: 51 546
Bpu10I CC'TnA_GC
Cuts at: 0 505 597
Size: 505 92
BsaAI yAC'GTr
Cuts at: 0 36 597
Size: 36 561
BsaHI Gr'CG_yC
Cuts at: 0 349 446 597
Size: 349 97 151
Fragments arranged by size:
349 151 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 597
Size: 88 509
BseYI C'CCAG_C
Cuts at: 0 387 597
Size: 387 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 597
Size: 477 120
BsiEI CG_ry'CG
Cuts at: 0 401 474 597
Size: 401 73 123
Fragments arranged by size:
401 123 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 328 597
Size: 328 269
Bsp1286I G_dGCh'C
Cuts at: 0 130 157 199 597
Size: 130 27 42 398
Fragments arranged by size:
398 130 42 27
BsrBI CCG'CTC
Cuts at: 0 243 597
Size: 243 354
BsrFI r'CCGG_y
Cuts at: 0 306 597
Size: 306 291
BssHII G'CGCG_C
Cuts at: 0 29 597
Size: 29 568
BstAPI GCAn_nnn'nTGC
Cuts at: 0 500 597
Size: 500 97
BtgI C'CryG_G
Cuts at: 0 200 428 589 597
Size: 200 228 161 8
Fragments arranged by size:
228 200 161 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 345 597
Size: 345 252
BtsI GCAGTG_nn'
Cuts at: 0 441 597
Size: 441 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 507 542 597
Size: 507 35 55
Fragments arranged by size:
507 55 35
EaeI y'GGCC_r
Cuts at: 0 227 371 398 471 528 597
Size: 227 144 27 73 57 69
Fragments arranged by size:
227 144 73 69 57 27
EagI C'GGCC_G
Cuts at: 0 398 471 597
Size: 398 73 126
Fragments arranged by size:
398 126 73
EcoO109I rG'GnC_Cy
Cuts at: 0 126 154 196 597
Size: 126 28 42 401
Fragments arranged by size:
401 126 42 28
FspI TGC'GCA
Cuts at: 0 286 597
Size: 286 311
HaeII r_GCGC'y
Cuts at: 0 283 449 597
Size: 283 166 148
Fragments arranged by size:
283 166 148
KasI G'GCGC_C
Cuts at: 0 445 597
Size: 445 152
MluI A'CGCG_T
Cuts at: 0 95 597
Size: 95 502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 597
Size: 80 517
MscI TGG'CCA
Cuts at: 0 373 597
Size: 373 224
MspA1I CmG'CkG
Cuts at: 0 219 430 551 597
Size: 219 211 121 46
Fragments arranged by size:
219 211 121 46
NarI GG'CG_CC
Cuts at: 0 446 597
Size: 446 151
NotI GC'GGCC_GC
Cuts at: 0 471 597
Size: 471 126
PmlI CAC'GTG
Cuts at: 0 36 597
Size: 36 561
PspOMI G'GGCC_C
Cuts at: 0 126 153 195 597
Size: 126 27 42 402
Fragments arranged by size:
402 126 42 27
PvuII CAG'CTG
Cuts at: 0 551 597
Size: 551 46
SacII CC_GC'GG
Cuts at: 0 431 597
Size: 431 166
SfoI GGC'GCC
Cuts at: 0 447 597
Size: 447 150
SmaI CCC'GGG
Cuts at: 0 152 159 597
Size: 152 7 438
Fragments arranged by size:
438 152 7
SrfI GCCC'GGGC
Cuts at: 0 152 597
Size: 152 445
StyI C'CwwG_G
Cuts at: 0 440 597
Size: 440 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 597
Size: 306 291
XmaI C'CCGG_G
Cuts at: 0 150 157 597
Size: 150 7 440
Fragments arranged by size:
440 150 7
Enzymes that do cut:
AflIII AloI ApaI AvaI BanI BanII BbeI BbvCI
BglI BlpI Bme1580I BmgBI Bpu10I BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsmBI Bsp1286I BsrBI BsrFI BssHII
BstAPI BtgI BtgZI BtsI CspCI EaeI EagI EcoO109I
FspI HaeII KasI MluI MmeI MscI MspA1I NarI
NotI PmlI PspOMI PvuII SacII SfoI SmaI SrfI
StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BmrI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI DraI DraIII DrdI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MslI
NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI
ZraI