Restriction map for
pFN21AB9659
(Linear) (Six-base) MAPSORT of: KIBB9659.seq Check: 3733 from: 1 to: 1464
KIBB9659.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1464
Size: 146 1318
AgeI A'CCGG_T
Cuts at: 0 757 1464
Size: 757 707
AleI CACnn'nnGTG
Cuts at: 0 506 590 926 1262 1464
Size: 506 84 336 336 202
Fragments arranged by size:
506 336 336 202 84
ApoI r'AATT_y
Cuts at: 0 161 245 329 413 581 749 833 917
Size: 161 84 84 84 168 168 84 84
Cuts at: 917 1085 1337 1464
Size: 168 252 127
Fragments arranged by size:
252 168 168 168 161 127 84 84
84 84 84
BanI G'GyrC_C
Cuts at: 0 1244 1464
Size: 1244 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1051 1464
Size: 134 917 413
Fragments arranged by size:
917 413 134
BciVI GTATCCnnnnn_n'
Cuts at: 0 157 1464
Size: 157 1307
BfrBI ATG'CAT
Cuts at: 0 1005 1464
Size: 1005 459
BglII A'GATC_T
Cuts at: 0 897 1464
Size: 897 567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1464
Size: 1031 433
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1299 1464
Size: 1299 165
BsaWI w'CCGG_w
Cuts at: 0 757 1464
Size: 757 707
BsmI GAATG_Cn'
Cuts at: 0 277 697 1007 1464
Size: 277 420 310 457
Fragments arranged by size:
457 420 310 277
BsrFI r'CCGG_y
Cuts at: 0 757 1464
Size: 757 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 313 1464
Size: 313 1151
BstYI r'GATC_y
Cuts at: 0 85 897 1464
Size: 85 812 567
Fragments arranged by size:
812 567 85
BtsI GCAGTG_nn'
Cuts at: 0 642 726 1464
Size: 642 84 738
Fragments arranged by size:
738 642 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 264 299 684 719 1464
Size: 264 35 385 35 745
Fragments arranged by size:
745 385 264 35 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 678 1464
Size: 678 786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1031 1464
Size: 146 885 433
Fragments arranged by size:
885 433 146
EcoRI G'AATT_C
Cuts at: 0 161 245 329 413 581 749 833 917
Size: 161 84 84 84 168 168 84 84
Cuts at: 917 1085 1337 1464
Size: 168 252 127
Fragments arranged by size:
252 168 168 168 161 127 84 84
84 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 479 511 1464
Size: 105 32 342 32 953
Fragments arranged by size:
953 342 105 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1182 1464
Size: 1182 282
MslI CAynn'nnrTG
Cuts at: 0 104 170 254 338 422 506 590 674
Size: 104 66 84 84 84 84 84 84
Cuts at: 674 758 774 842 926 1094 1178 1262 1346
Size: 84 16 68 84 168 84 84 84
Cuts at: 1346 1464
Size: 118
Fragments arranged by size:
168 118 104 84 84 84 84 84
84 84 84 84 84 84 68 66
16
NsiI A_TGCA'T
Cuts at: 0 1007 1464
Size: 1007 457
NspI r_CATG'y
Cuts at: 0 135 704 1464
Size: 135 569 760
Fragments arranged by size:
760 569 135
SmlI C'TyrA_G
Cuts at: 0 1314 1464
Size: 1314 150
StuI AGG'CCT
Cuts at: 0 292 376 479 796 880 1132 1464
Size: 292 84 103 317 84 252 332
Fragments arranged by size:
332 317 292 252 103 84 84
XmnI GAAnn'nnTTC
Cuts at: 0 975 1464
Size: 975 489
Enzymes that do cut:
AcuI AgeI AleI ApoI BanI BbsI BciVI BfrBI
BglII BpmI BpuEI BsaWI BsmI BsrFI BstAPI BstYI
BtsI CspCI EciI Eco57MI EcoRI Hin4I MmeI MslI
NsiI NspI SmlI StuI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI
DraIII DrdI EaeI EagI EarI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI