Restriction map for
pFN21AB9650
(Linear) (Six-base) MAPSORT of: KIBB9650.seq Check: 9197 from: 1 to: 945
KIBB9650.orf
With 182 enzymes: SgfI *
March 22, 2012 15:46 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 240 709 945
Size: 240 469 236
Fragments arranged by size:
469 240 236
AvaI C'yCGr_G
Cuts at: 0 51 58 105 412 484 543 587 837
Size: 51 7 47 307 72 59 44 250
Cuts at: 837 877 945
Size: 40 68
Fragments arranged by size:
307 250 72 68 59 51 47 44
40 7
BanI G'GyrC_C
Cuts at: 0 351 480 908 945
Size: 351 129 428 37
Fragments arranged by size:
428 351 129 37
BanII G_rGCy'C
Cuts at: 0 89 945
Size: 89 856
BbeI G_GCGC'C
Cuts at: 0 355 484 912 945
Size: 355 129 428 33
Fragments arranged by size:
428 355 129 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 945
Size: 506 439
BbvCI CC'TCA_GC
Cuts at: 0 710 945
Size: 710 235
BglI GCCn_nnn'nGGC
Cuts at: 0 437 788 945
Size: 437 351 157
Fragments arranged by size:
437 351 157
BglII A'GATC_T
Cuts at: 0 206 945
Size: 206 739
Bme1580I G_kGCm'C
Cuts at: 0 844 945
Size: 844 101
BmgBI CAC'GTC
Cuts at: 0 845 945
Size: 845 100
Bpu10I CC'TnA_GC
Cuts at: 0 710 945
Size: 710 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 391 945
Size: 277 114 554
Fragments arranged by size:
554 277 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 945
Size: 532 413
BsaHI Gr'CG_yC
Cuts at: 0 352 481 736 909 945
Size: 352 129 255 173 36
Fragments arranged by size:
352 255 173 129 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 526 688 703 706 709 739 760
Size: 85 441 162 15 3 3 30 21
Cuts at: 760 945
Size: 185
Fragments arranged by size:
441 185 162 85 30 21 15 3
3
BseYI C'CCAG_C
Cuts at: 0 753 945
Size: 753 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 855 945
Size: 25 830 90
Fragments arranged by size:
830 90 25
BsiEI CG_ry'CG
Cuts at: 0 68 331 635 788 945
Size: 68 263 304 153 157
Fragments arranged by size:
304 263 157 153 68
BsmI GAATG_Cn'
Cuts at: 0 136 945
Size: 136 809
BsmBI CGTCTCn'nnnn_
Cuts at: 0 721 945
Size: 721 224
Bsp1286I G_dGCh'C
Cuts at: 0 89 844 945
Size: 89 755 101
Fragments arranged by size:
755 101 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 945
Size: 833 112
BsrBI CCG'CTC
Cuts at: 0 110 282 586 945
Size: 110 172 304 359
Fragments arranged by size:
359 304 172 110
BsrFI r'CCGG_y
Cuts at: 0 63 326 401 787 945
Size: 63 263 75 386 158
Fragments arranged by size:
386 263 158 75 63
BssHII G'CGCG_C
Cuts at: 0 79 603 945
Size: 79 524 342
Fragments arranged by size:
524 342 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 240 945
Size: 240 705
BstEII G'GTnAC_C
Cuts at: 0 882 945
Size: 882 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 945
Size: 219 726
BstYI r'GATC_y
Cuts at: 0 206 257 945
Size: 206 51 688
Fragments arranged by size:
688 206 51
BtgI C'CryG_G
Cuts at: 0 432 945
Size: 432 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 906 945
Size: 906 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 735 945
Size: 735 210
EaeI y'GGCC_r
Cuts at: 0 65 111 328 429 632 785 945
Size: 65 46 217 101 203 153 160
Fragments arranged by size:
217 203 160 153 101 65 46
EagI C'GGCC_G
Cuts at: 0 65 328 632 785 945
Size: 65 263 304 153 160
Fragments arranged by size:
304 263 160 153 65
EarI CTCTTCn'nnn_
Cuts at: 0 680 716 945
Size: 680 36 229
Fragments arranged by size:
680 229 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 180 292 319 945
Size: 180 112 27 626
Fragments arranged by size:
626 180 112 27
EcoO109I rG'GnC_Cy
Cuts at: 0 55 457 945
Size: 55 402 488
Fragments arranged by size:
488 402 55
FseI GG_CCGG'CC
Cuts at: 0 67 791 945
Size: 67 724 154
Fragments arranged by size:
724 154 67
HaeII r_GCGC'y
Cuts at: 0 219 355 484 806 912 945
Size: 219 136 129 322 106 33
Fragments arranged by size:
322 219 136 129 106 33
KasI G'GCGC_C
Cuts at: 0 351 480 908 945
Size: 351 129 428 37
Fragments arranged by size:
428 351 129 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 945
Size: 644 301
MspA1I CmG'CkG
Cuts at: 0 12 230 237 423 434 451 706 945
Size: 12 218 7 186 11 17 255 239
Fragments arranged by size:
255 239 218 186 17 12 11 7
NaeI GCC'GGC
Cuts at: 0 65 403 789 945
Size: 65 338 386 156
Fragments arranged by size:
386 338 156 65
NarI GG'CG_CC
Cuts at: 0 352 481 909 945
Size: 352 129 428 36
Fragments arranged by size:
428 352 129 36
NgoMIV G'CCGG_C
Cuts at: 0 63 401 787 945
Size: 63 338 386 158
Fragments arranged by size:
386 338 158 63
NotI GC'GGCC_GC
Cuts at: 0 632 945
Size: 632 313
NruI TCG'CGA
Cuts at: 0 815 945
Size: 815 130
NspI r_CATG'y
Cuts at: 0 195 945
Size: 195 750
PfoI T'CCnGG_A
Cuts at: 0 860 945
Size: 860 85
PpuMI rG'GwC_Cy
Cuts at: 0 55 945
Size: 55 890
PstI C_TGCA'G
Cuts at: 0 245 802 830 945
Size: 245 557 28 115
Fragments arranged by size:
557 245 115 28
PvuII CAG'CTG
Cuts at: 0 237 423 945
Size: 237 186 522
Fragments arranged by size:
522 237 186
SacII CC_GC'GG
Cuts at: 0 435 945
Size: 435 510
SanDI GG'GwC_CC
Cuts at: 0 55 945
Size: 55 890
SexAI A'CCwGG_T
Cuts at: 0 929 945
Size: 929 16
SfcI C'TryA_G
Cuts at: 0 241 798 826 945
Size: 241 557 28 119
Fragments arranged by size:
557 241 119 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 437 788 945
Size: 437 351 157
Fragments arranged by size:
437 351 157
SfoI GGC'GCC
Cuts at: 0 353 482 910 945
Size: 353 129 428 35
Fragments arranged by size:
428 353 129 35
SmaI CCC'GGG
Cuts at: 0 53 60 414 545 945
Size: 53 7 354 131 400
Fragments arranged by size:
400 354 131 53 7
SmlI C'TyrA_G
Cuts at: 0 292 406 945
Size: 292 114 539
Fragments arranged by size:
539 292 114
SrfI GCCC'GGGC
Cuts at: 0 414 945
Size: 414 531
StyI C'CwwG_G
Cuts at: 0 359 609 945
Size: 359 250 336
Fragments arranged by size:
359 336 250
Tth111I GACn'n_nGTC
Cuts at: 0 660 945
Size: 660 285
XmaI C'CCGG_G
Cuts at: 0 51 58 412 543 945
Size: 51 7 354 131 402
Fragments arranged by size:
402 354 131 51 7
XmnI GAAnn'nnTTC
Cuts at: 0 136 945
Size: 136 809
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI
DrdI EaeI EagI EarI EciI EcoO109I FseI HaeII
KasI MmeI MspA1I NaeI NarI NgoMIV NotI NruI
NspI PfoI PpuMI PstI PvuII SacII SanDI SexAI
SfcI SfiI SfoI SmaI SmlI SrfI StyI Tth111I
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BlpI BmrI BmtI BplI BpmI
BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI TaqII TatI TstI XbaI XcmI
XhoI ZraI