Restriction map for
pFN21AB9604
(Linear) (Six-base) MAPSORT of: KIBB9604.seq Check: 8282 from: 1 to: 1128
KIBB9604.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AflIII A'CryG_T
Cuts at: 0 407 777 1128
Size: 407 370 351
Fragments arranged by size:
407 370 351
AgeI A'CCGG_T
Cuts at: 0 58 1128
Size: 58 1070
AleI CACnn'nnGTG
Cuts at: 0 778 1128
Size: 778 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 540 811 1128
Size: 540 271 317
Fragments arranged by size:
540 317 271
AlwNI CAG_nnn'CTG
Cuts at: 0 676 685 1128
Size: 676 9 443
Fragments arranged by size:
676 443 9
ApaI G_GGCC'C
Cuts at: 0 42 706 751 1128
Size: 42 664 45 377
Fragments arranged by size:
664 377 45 42
AvaI C'yCGr_G
Cuts at: 0 16 22 1051 1128
Size: 16 6 1029 77
Fragments arranged by size:
1029 77 16 6
BanI G'GyrC_C
Cuts at: 0 342 431 511 538 567 999 1128
Size: 342 89 80 27 29 432 129
Fragments arranged by size:
432 342 129 89 80 29 27
BanII G_rGCy'C
Cuts at: 0 23 42 100 497 706 751 1058 1128
Size: 23 19 58 397 209 45 307 70
Fragments arranged by size:
397 307 209 70 58 45 23 19
BbeI G_GCGC'C
Cuts at: 0 435 515 542 571 1003 1128
Size: 435 80 27 29 432 125
Fragments arranged by size:
435 432 125 80 29 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 1128
Size: 410 718
BbvCI CC'TCA_GC
Cuts at: 0 316 1128
Size: 316 812
BfrBI ATG'CAT
Cuts at: 0 1068 1128
Size: 1068 60
BglI GCCn_nnn'nGGC
Cuts at: 0 46 84 459 673 833 1128
Size: 46 38 375 214 160 295
Fragments arranged by size:
375 295 214 160 46 38
BlpI GC'TnA_GC
Cuts at: 0 964 1128
Size: 964 164
Bme1580I G_kGCm'C
Cuts at: 0 42 706 751 1044 1128
Size: 42 664 45 293 84
Fragments arranged by size:
664 293 84 45 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 1089 1128
Size: 1089 39
Bpu10I CC'TnA_GC
Cuts at: 0 316 1128
Size: 316 812
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 1128
Size: 49 1079
BsaAI yAC'GTr
Cuts at: 0 177 1128
Size: 177 951
BsaHI Gr'CG_yC
Cuts at: 0 432 512 539 568 1000 1128
Size: 432 80 27 29 432 128
Fragments arranged by size:
432 432 128 80 29 27
BsaWI w'CCGG_w
Cuts at: 0 58 1128
Size: 58 1070
BseYI C'CCAG_C
Cuts at: 0 978 1128
Size: 978 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 880 1128
Size: 802 78 248
Fragments arranged by size:
802 248 78
BsiEI CG_ry'CG
Cuts at: 0 63 463 725 755 845 905 1128
Size: 63 400 262 30 90 60 223
Fragments arranged by size:
400 262 223 90 63 60 30
BsiHKAI G_wGCw'C
Cuts at: 0 1058 1128
Size: 1058 70
BsiWI C'GTAC_G
Cuts at: 0 173 761 1128
Size: 173 588 367
Fragments arranged by size:
588 367 173
BsmI GAATG_Cn'
Cuts at: 0 1110 1128
Size: 1110 18
Bsp1286I G_dGCh'C
Cuts at: 0 23 42 100 497 706 751 1044 1058
Size: 23 19 58 397 209 45 293 14
Cuts at: 1058 1128
Size: 70
Fragments arranged by size:
397 293 209 70 58 45 23 19
14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 590 1128
Size: 590 538
BsrDI GCAATG_nn'
Cuts at: 0 1068 1128
Size: 1068 60
BsrFI r'CCGG_y
Cuts at: 0 58 428 840 1099 1128
Size: 58 370 412 259 29
Fragments arranged by size:
412 370 259 58 29
BssHII G'CGCG_C
Cuts at: 0 1103 1128
Size: 1103 25
BssSI C'ACGA_G
Cuts at: 0 606 1128
Size: 606 522
BstAPI GCAn_nnn'nTGC
Cuts at: 0 676 685 1128
Size: 676 9 443
Fragments arranged by size:
676 443 9
BtgI C'CryG_G
Cuts at: 0 191 698 933 1128
Size: 191 507 235 195
Fragments arranged by size:
507 235 195 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 237 1128
Size: 171 66 891
Fragments arranged by size:
891 171 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 93 128 1128
Size: 93 35 1000
Fragments arranged by size:
1000 93 35
EaeI y'GGCC_r
Cuts at: 0 5 460 610 722 752 842 902 1128
Size: 5 455 150 112 30 90 60 226
Fragments arranged by size:
455 226 150 112 90 60 30 5
EagI C'GGCC_G
Cuts at: 0 460 722 752 842 902 1128
Size: 460 262 30 90 60 226
Fragments arranged by size:
460 262 226 90 60 30
EarI CTCTTCn'nnn_
Cuts at: 0 211 442 502 1128
Size: 211 231 60 626
Fragments arranged by size:
626 231 211 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 295 848 1128
Size: 295 553 280
Fragments arranged by size:
553 295 280
EcoICRI GAG'CTC
Cuts at: 0 1056 1128
Size: 1056 72
EcoO109I rG'GnC_Cy
Cuts at: 0 38 39 452 703 1128
Size: 38 1 413 251 425
Fragments arranged by size:
425 413 251 38 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 513 1128
Size: 471 42 615
Fragments arranged by size:
615 471 42
HaeII r_GCGC'y
Cuts at: 0 184 435 515 542 571 796 1003 1128
Size: 184 251 80 27 29 225 207 125
Fragments arranged by size:
251 225 207 184 125 80 29 27
KasI G'GCGC_C
Cuts at: 0 431 511 538 567 999 1128
Size: 431 80 27 29 432 129
Fragments arranged by size:
432 431 129 80 29 27
MluI A'CGCG_T
Cuts at: 0 777 1128
Size: 777 351
MscI TGG'CCA
Cuts at: 0 7 1128
Size: 7 1121
MslI CAynn'nnrTG
Cuts at: 0 190 340 778 1128
Size: 190 150 438 350
Fragments arranged by size:
438 350 190 150
MspA1I CmG'CkG
Cuts at: 0 673 685 700 727 1128
Size: 673 12 15 27 401
Fragments arranged by size:
673 401 27 15 12
NaeI GCC'GGC
Cuts at: 0 842 1128
Size: 842 286
NarI GG'CG_CC
Cuts at: 0 432 512 539 568 1000 1128
Size: 432 80 27 29 432 128
Fragments arranged by size:
432 432 128 80 29 27
NcoI C'CATG_G
Cuts at: 0 191 1128
Size: 191 937
NgoMIV G'CCGG_C
Cuts at: 0 840 1128
Size: 840 288
NotI GC'GGCC_GC
Cuts at: 0 722 902 1128
Size: 722 180 226
Fragments arranged by size:
722 226 180
NruI TCG'CGA
Cuts at: 0 256 1128
Size: 256 872
NsiI A_TGCA'T
Cuts at: 0 1070 1128
Size: 1070 58
NspI r_CATG'y
Cuts at: 0 411 1128
Size: 411 717
PciI A'CATG_T
Cuts at: 0 407 1128
Size: 407 721
PspOMI G'GGCC_C
Cuts at: 0 38 702 747 1128
Size: 38 664 45 381
Fragments arranged by size:
664 381 45 38
PstI C_TGCA'G
Cuts at: 0 587 681 696 1031 1128
Size: 587 94 15 335 97
Fragments arranged by size:
587 335 97 94 15
PvuII CAG'CTG
Cuts at: 0 685 1128
Size: 685 443
SacI G_AGCT'C
Cuts at: 0 1058 1128
Size: 1058 70
SacII CC_GC'GG
Cuts at: 0 701 1128
Size: 701 427
SapI GCTCTTCn'nnn_
Cuts at: 0 502 1128
Size: 502 626
SfcI C'TryA_G
Cuts at: 0 583 677 692 1027 1128
Size: 583 94 15 335 101
Fragments arranged by size:
583 335 101 94 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 46 459 833 1128
Size: 46 413 374 295
Fragments arranged by size:
413 374 295 46
SfoI GGC'GCC
Cuts at: 0 433 513 540 569 1001 1128
Size: 433 80 27 29 432 127
Fragments arranged by size:
433 432 127 80 29 27
StyI C'CwwG_G
Cuts at: 0 191 1128
Size: 191 937
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 1128
Size: 648 480
TatI w'GTAC_w
Cuts at: 0 245 1128
Size: 245 883
Enzymes that do cut:
AflIII AgeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmrI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrDI BsrFI
BssHII BssSI BstAPI BtgI BtgZI CspCI EaeI EagI
EarI EciI EcoICRI EcoO109I FalI HaeII KasI MluI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI PciI PspOMI PstI PvuII SacI
SacII SapI SfcI SfiI SfoI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BglII BmgBI BmtI
BplI BpmI BpuEI BsaBI BsaXI BseRI BsmBI BspEI
BspHI BsrBI BsrGI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI Eco57MI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MmeI NdeI NheI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI