Restriction map for
pFN21AB9597
(Linear) (Six-base) MAPSORT of: KIBB9597.seq Check: 6395 from: 1 to: 1491
KIBB9597.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
Acc65I G'GTAC_C
Cuts at: 0 933 1491
Size: 933 558
AfeI AGC'GCT
Cuts at: 0 664 1491
Size: 664 827
AflIII A'CryG_T
Cuts at: 0 626 1491
Size: 626 865
AhdI GACnn_n'nnGTC
Cuts at: 0 615 1491
Size: 615 876
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 1491
Size: 664 827
AlwNI CAG_nnn'CTG
Cuts at: 0 17 681 1015 1491
Size: 17 664 334 476
Fragments arranged by size:
664 476 334 17
ApaI G_GGCC'C
Cuts at: 0 134 329 1039 1045 1051 1491
Size: 134 195 710 6 6 440
Fragments arranged by size:
710 440 195 134 6 6
AvaI C'yCGr_G
Cuts at: 0 143 260 288 328 582 1032 1038 1044
Size: 143 117 28 40 254 450 6 6
Cuts at: 1044 1287 1420 1491
Size: 243 133 71
Fragments arranged by size:
450 254 243 143 133 117 71 40
28 6 6
BamHI G'GATC_C
Cuts at: 0 753 1491
Size: 753 738
BanI G'GyrC_C
Cuts at: 0 298 790 933 1135 1213 1291 1491
Size: 298 492 143 202 78 78 200
Fragments arranged by size:
492 298 202 200 143 78 78
BanII G_rGCy'C
Cuts at: 0 46 116 134 245 289 329 370 430
Size: 46 70 18 111 44 40 41 60
Cuts at: 430 1039 1045 1051 1306 1491
Size: 609 6 6 255 185
Fragments arranged by size:
609 255 185 111 70 60 46 44
41 40 18 6 6
BbeI G_GCGC'C
Cuts at: 0 302 794 1217 1295 1491
Size: 302 492 423 78 196
Fragments arranged by size:
492 423 302 196 78
BglI GCCn_nnn'nGGC
Cuts at: 0 307 745 1055 1268 1478 1491
Size: 307 438 310 213 210 13
Fragments arranged by size:
438 310 307 213 210 13
BglII A'GATC_T
Cuts at: 0 455 1491
Size: 455 1036
Bme1580I G_kGCm'C
Cuts at: 0 134 329 1039 1045 1051 1491
Size: 134 195 710 6 6 440
Fragments arranged by size:
710 440 195 134 6 6
BmgBI CAC'GTC
Cuts at: 0 84 150 1491
Size: 84 66 1341
Fragments arranged by size:
1341 84 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1491
Size: 1162 329
BsaHI Gr'CG_yC
Cuts at: 0 256 299 654 791 1111 1214 1292 1491
Size: 256 43 355 137 320 103 78 199
Fragments arranged by size:
355 320 256 199 137 103 78 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 229 1491
Size: 226 3 1262
Fragments arranged by size:
1262 226 3
BseYI C'CCAG_C
Cuts at: 0 117 1008 1083 1491
Size: 117 891 75 408
Fragments arranged by size:
891 408 117 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1491
Size: 1220 271
BsiEI CG_ry'CG
Cuts at: 0 53 275 477 623 1491
Size: 53 222 202 146 868
Fragments arranged by size:
868 222 202 146 53
BsiHKAI G_wGCw'C
Cuts at: 0 46 116 155 1491
Size: 46 70 39 1336
Fragments arranged by size:
1336 70 46 39
Bsp1286I G_dGCh'C
Cuts at: 0 46 116 134 155 245 289 329 370
Size: 46 70 18 21 90 44 40 41
Cuts at: 370 430 1039 1045 1051 1306 1491
Size: 60 609 6 6 255 185
Fragments arranged by size:
609 255 185 90 70 60 46 44
41 40 21 18 6 6
BsrBI CCG'CTC
Cuts at: 0 916 1491
Size: 916 575
BsrFI r'CCGG_y
Cuts at: 0 849 1132 1294 1468 1491
Size: 849 283 162 174 23
Fragments arranged by size:
849 283 174 162 23
BssSI C'ACGA_G
Cuts at: 0 166 1491
Size: 166 1325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 17 681 1491
Size: 17 664 810
Fragments arranged by size:
810 664 17
BstYI r'GATC_y
Cuts at: 0 455 560 753 1491
Size: 455 105 193 738
Fragments arranged by size:
738 455 193 105
Bsu36I CC'TnA_GG
Cuts at: 0 1026 1440 1491
Size: 1026 414 51
Fragments arranged by size:
1026 414 51
BtgI C'CryG_G
Cuts at: 0 159 176 302 312 410 740 899 1139
Size: 159 17 126 10 98 330 159 240
Cuts at: 1139 1263 1331 1491
Size: 124 68 160
Fragments arranged by size:
330 240 160 159 159 126 124 98
68 17 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 381 1491
Size: 381 1110
DrdI GACnn_nn'nnGTC
Cuts at: 0 553 1491
Size: 553 938
EaeI y'GGCC_r
Cuts at: 0 50 162 272 413 474 620 1361 1398
Size: 50 112 110 141 61 146 741 37
Cuts at: 1398 1491
Size: 93
Fragments arranged by size:
741 146 141 112 110 93 61 50
37
EagI C'GGCC_G
Cuts at: 0 50 272 474 620 1491
Size: 50 222 202 146 871
Fragments arranged by size:
871 222 202 146 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 1491
Size: 514 977
EcoICRI GAG'CTC
Cuts at: 0 44 114 1491
Size: 44 70 1377
Fragments arranged by size:
1377 70 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1025 1491
Size: 1025 466
EcoO109I rG'GnC_Cy
Cuts at: 0 131 325 1048 1173 1336 1445 1491
Size: 131 194 723 125 163 109 46
Fragments arranged by size:
723 194 163 131 125 109 46
FseI GG_CCGG'CC
Cuts at: 0 853 1491
Size: 853 638
HaeII r_GCGC'y
Cuts at: 0 302 666 764 794 1217 1295 1491
Size: 302 364 98 30 423 78 196
Fragments arranged by size:
423 364 302 196 98 78 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 202 234 601 633 1491
Size: 16 32 154 32 367 32 858
Fragments arranged by size:
858 367 154 32 32 32 16
KasI G'GCGC_C
Cuts at: 0 298 790 1213 1291 1491
Size: 298 492 423 78 200
Fragments arranged by size:
492 423 298 200 78
KpnI G_GTAC'C
Cuts at: 0 937 1491
Size: 937 554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1491
Size: 997 494
MscI TGG'CCA
Cuts at: 0 415 1363 1400 1491
Size: 415 948 37 91
Fragments arranged by size:
948 415 91 37
MslI CAynn'nnrTG
Cuts at: 0 82 409 1491
Size: 82 327 1082
Fragments arranged by size:
1082 327 82
MspA1I CmG'CkG
Cuts at: 0 14 101 178 280 304 314 322 372
Size: 14 87 77 102 24 10 8 50
Cuts at: 372 473 742 901 927 1141 1333 1491
Size: 101 269 159 26 214 192 158
Fragments arranged by size:
269 214 192 159 158 102 101 87
77 50 26 24 14 10 8
NaeI GCC'GGC
Cuts at: 0 851 1134 1296 1470 1491
Size: 851 283 162 174 21
Fragments arranged by size:
851 283 174 162 21
NarI GG'CG_CC
Cuts at: 0 299 791 1214 1292 1491
Size: 299 492 423 78 199
Fragments arranged by size:
492 423 299 199 78
NcoI C'CATG_G
Cuts at: 0 410 1491
Size: 410 1081
NgoMIV G'CCGG_C
Cuts at: 0 849 1132 1294 1468 1491
Size: 849 283 162 174 23
Fragments arranged by size:
849 283 174 162 23
NotI GC'GGCC_GC
Cuts at: 0 272 474 1491
Size: 272 202 1017
Fragments arranged by size:
1017 272 202
NspI r_CATG'y
Cuts at: 0 630 1491
Size: 630 861
PasI CC'CwG_GG
Cuts at: 0 6 1491
Size: 6 1485
PciI A'CATG_T
Cuts at: 0 626 1491
Size: 626 865
PflMI CCAn_nnn'nTGG
Cuts at: 0 1257 1359 1491
Size: 1257 102 132
Fragments arranged by size:
1257 132 102
PfoI T'CCnGG_A
Cuts at: 0 1308 1491
Size: 1308 183
PpuMI rG'GwC_Cy
Cuts at: 0 1336 1445 1491
Size: 1336 109 46
Fragments arranged by size:
1336 109 46
PshAI GACnn'nnGTC
Cuts at: 0 342 1491
Size: 342 1149
PspOMI G'GGCC_C
Cuts at: 0 130 325 1035 1041 1047 1491
Size: 130 195 710 6 6 444
Fragments arranged by size:
710 444 195 130 6 6
PstI C_TGCA'G
Cuts at: 0 425 1491
Size: 425 1066
PvuII CAG'CTG
Cuts at: 0 14 927 1491
Size: 14 913 564
Fragments arranged by size:
913 564 14
SacI G_AGCT'C
Cuts at: 0 46 116 1491
Size: 46 70 1375
Fragments arranged by size:
1375 70 46
SacII CC_GC'GG
Cuts at: 0 179 305 315 743 902 1142 1334 1491
Size: 179 126 10 428 159 240 192 157
Fragments arranged by size:
428 240 192 179 159 157 126 10
SanDI GG'GwC_CC
Cuts at: 0 1445 1491
Size: 1445 46
SfcI C'TryA_G
Cuts at: 0 421 1244 1491
Size: 421 823 247
Fragments arranged by size:
823 421 247
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1055 1491
Size: 1055 436
SfoI GGC'GCC
Cuts at: 0 300 792 1215 1293 1491
Size: 300 492 423 78 198
Fragments arranged by size:
492 423 300 198 78
SgrAI Cr'CCGG_yG
Cuts at: 0 1294 1491
Size: 1294 197
SmaI CCC'GGG
Cuts at: 0 145 262 290 330 584 1040 1046 1289
Size: 145 117 28 40 254 456 6 243
Cuts at: 1289 1422 1491
Size: 133 69
Fragments arranged by size:
456 254 243 145 133 117 69 40
28 6
SmlI C'TyrA_G
Cuts at: 0 1177 1491
Size: 1177 314
SrfI GCCC'GGGC
Cuts at: 0 330 1040 1046 1491
Size: 330 710 6 445
Fragments arranged by size:
710 445 330 6
StuI AGG'CCT
Cuts at: 0 1058 1233 1491
Size: 1058 175 258
Fragments arranged by size:
1058 258 175
StyI C'CwwG_G
Cuts at: 0 410 1491
Size: 410 1081
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 710 1491
Size: 710 781
XmaI C'CCGG_G
Cuts at: 0 143 260 288 328 582 1038 1044 1287
Size: 143 117 28 40 254 456 6 243
Cuts at: 1287 1420 1491
Size: 133 71
Fragments arranged by size:
456 254 243 143 133 117 71 40
28 6
Enzymes that do cut:
Acc65I AfeI AflIII AhdI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BglI BglII Bme1580I BmgBI
BpuEI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrBI BsrFI BssSI BstAPI BstYI Bsu36I BtgI BtgZI
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I FseI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SfcI SfiI SfoI SgrAI SmaI SmlI
SrfI StuI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII EarI Eco57MI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI MfeI MluI NdeI
NheI NruI NsiI PacI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SexAI SgfI SnaBI SpeI SphI SspI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI